Restriction map for
pFN21AE9570
(Linear) (Six-base) MAPSORT of: KIEE9570.seq Check: 2393 from: 1 to: 1140
KIEE9570.orf
With 182 enzymes: SgfI *
December 27, 2011 16:42 ..
AatII G_ACGT'C
Cuts at: 0 1004 1140
Size: 1004 136
AccI GT'mk_AC
Cuts at: 0 236 1140
Size: 236 904
AfeI AGC'GCT
Cuts at: 0 699 1140
Size: 699 441
AflII C'TTAA_G
Cuts at: 0 693 1140
Size: 693 447
AflIII A'CryG_T
Cuts at: 0 104 1140
Size: 104 1036
AleI CACnn'nnGTG
Cuts at: 0 46 1140
Size: 46 1094
AlwNI CAG_nnn'CTG
Cuts at: 0 213 868 1140
Size: 213 655 272
Fragments arranged by size:
655 272 213
ApaLI G'TGCA_C
Cuts at: 0 156 1087 1140
Size: 156 931 53
Fragments arranged by size:
931 156 53
AvrII C'CTAG_G
Cuts at: 0 327 1140
Size: 327 813
BanI G'GyrC_C
Cuts at: 0 168 1140
Size: 168 972
BbsI GAAGACnn'nnnn_
Cuts at: 0 548 1000 1140
Size: 548 452 140
Fragments arranged by size:
548 452 140
BglI GCCn_nnn'nGGC
Cuts at: 0 67 709 1140
Size: 67 642 431
Fragments arranged by size:
642 431 67
Bme1580I G_kGCm'C
Cuts at: 0 7 160 1091 1140
Size: 7 153 931 49
Fragments arranged by size:
931 153 49 7
BmrI ACTGGGnnnn_n'
Cuts at: 0 40 1140
Size: 40 1100
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 463 1062 1140
Size: 463 599 78
Fragments arranged by size:
599 463 78
BsaI GGTCTCn'nnnn_
Cuts at: 0 544 1140
Size: 544 596
BsaAI yAC'GTr
Cuts at: 0 924 1140
Size: 924 216
BsaHI Gr'CG_yC
Cuts at: 0 1001 1140
Size: 1001 139
BsaWI w'CCGG_w
Cuts at: 0 659 1140
Size: 659 481
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1027 1057 1060 1090 1140
Size: 1027 30 3 30 50
Fragments arranged by size:
1027 50 30 30 3
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 775 833 1048 1140
Size: 775 58 215 92
Fragments arranged by size:
775 215 92 58
BseYI C'CCAG_C
Cuts at: 0 162 1140
Size: 162 978
BsiEI CG_ry'CG
Cuts at: 0 417 1140
Size: 417 723
BsiHKAI G_wGCw'C
Cuts at: 0 160 1091 1140
Size: 160 931 49
Fragments arranged by size:
931 160 49
Bsp1286I G_dGCh'C
Cuts at: 0 7 160 1091 1140
Size: 7 153 931 49
Fragments arranged by size:
931 153 49 7
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 103 198 248 562 1140
Size: 103 95 50 314 578
Fragments arranged by size:
578 314 103 95 50
BsrFI r'CCGG_y
Cuts at: 0 171 1140
Size: 171 969
BsrGI T'GTAC_A
Cuts at: 0 143 269 857 1140
Size: 143 126 588 283
Fragments arranged by size:
588 283 143 126
BstAPI GCAn_nnn'nTGC
Cuts at: 0 213 410 1140
Size: 213 197 730
Fragments arranged by size:
730 213 197
BstBI TT'CG_AA
Cuts at: 0 113 1140
Size: 113 1027
BstEII G'GTnAC_C
Cuts at: 0 348 1100 1140
Size: 348 752 40
Fragments arranged by size:
752 348 40
BstXI CCAn_nnnn'nTGG
Cuts at: 0 738 1140
Size: 738 402
BtgI C'CryG_G
Cuts at: 0 64 428 890 932 1049 1140
Size: 64 364 462 42 117 91
Fragments arranged by size:
462 364 117 91 64 42
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 627 717 1140
Size: 627 90 423
Fragments arranged by size:
627 423 90
BtsI GCAGTG_nn'
Cuts at: 0 56 1140
Size: 56 1084
EaeI y'GGCC_r
Cuts at: 0 173 230 425 728 929 1140
Size: 173 57 195 303 201 211
Fragments arranged by size:
303 211 201 195 173 57
EarI CTCTTCn'nnn_
Cuts at: 0 110 397 1140
Size: 110 287 743
Fragments arranged by size:
743 287 110
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 75 477 1140
Size: 75 402 663
Fragments arranged by size:
663 402 75
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 463 1062 1140
Size: 463 599 78
Fragments arranged by size:
599 463 78
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1077 1140
Size: 1077 63
HaeII r_GCGC'y
Cuts at: 0 202 701 1140
Size: 202 499 439
Fragments arranged by size:
499 439 202
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1027 1059 1140
Size: 1027 32 81
Fragments arranged by size:
1027 81 32
MscI TGG'CCA
Cuts at: 0 931 1140
Size: 931 209
MslI CAynn'nnrTG
Cuts at: 0 46 736 1140
Size: 46 690 404
Fragments arranged by size:
690 404 46
MspA1I CmG'CkG
Cuts at: 0 320 799 1140
Size: 320 479 341
Fragments arranged by size:
479 341 320
NaeI GCC'GGC
Cuts at: 0 173 1140
Size: 173 967
NgoMIV G'CCGG_C
Cuts at: 0 171 1140
Size: 171 969
NspI r_CATG'y
Cuts at: 0 567 1140
Size: 567 573
PasI CC'CwG_GG
Cuts at: 0 75 1140
Size: 75 1065
PshAI GACnn'nnGTC
Cuts at: 0 1000 1140
Size: 1000 140
PstI C_TGCA'G
Cuts at: 0 209 218 572 1043 1140
Size: 209 9 354 471 97
Fragments arranged by size:
471 354 209 97 9
SexAI A'CCwGG_T
Cuts at: 0 776 1140
Size: 776 364
SfcI C'TryA_G
Cuts at: 0 205 214 568 813 1039 1140
Size: 205 9 354 245 226 101
Fragments arranged by size:
354 245 226 205 101 9
SmlI C'TyrA_G
Cuts at: 0 693 1140
Size: 693 447
StuI AGG'CCT
Cuts at: 0 332 1140
Size: 332 808
StyI C'CwwG_G
Cuts at: 0 327 713 1140
Size: 327 386 427
Fragments arranged by size:
427 386 327
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1030 1106 1140
Size: 1030 76 34
Fragments arranged by size:
1030 76 34
TatI w'GTAC_w
Cuts at: 0 143 269 857 1140
Size: 143 126 588 283
Fragments arranged by size:
588 283 143 126
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 840 1140
Size: 840 300
ZraI GAC'GTC
Cuts at: 0 1002 1140
Size: 1002 138
Enzymes that do cut:
AatII AccI AfeI AflII AflIII AleI AlwNI ApaLI
AvrII BanI BbsI BglI Bme1580I BmrI BpmI BsaI
BsaAI BsaHI BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI
Bsp1286I BspMI BsrFI BsrGI BstAPI BstBI BstEII BstXI
BtgI BtgZI BtsI EaeI EarI EciI Eco57MI EcoNI
HaeII Hin4I MscI MslI MspA1I NaeI NgoMIV NspI
PasI PshAI PstI SexAI SfcI SmlI StuI StyI
TaqII TatI XcmI ZraI
Enzymes that do not cut:
AarI Acc65I AclI AcuI AgeI AhdI AlfI AloI
ApaI ApoI AscI AseI AsiSI AvaI BaeI BamHI
BanII BbeI BbvCI BcgI BciVI BclI BfrBI BglII
BlpI BmgBI BmtI BplI Bpu10I BpuEI BsaBI BsgI
BsiWI BsmI BsmBI BspEI BspHI BsrBI BsrDI BssHII
BssSI BstYI BstZ17I Bsu36I ClaI CspCI DraI DraIII
DrdI EagI EcoICRI EcoO109I EcoRI EcoRV FalI FseI
FspI FspAI HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI NarI NcoI NdeI NheI NotI NruI
NsiI PacI PciI PflMI PfoI PmeI PmlI PpiI
PpuMI PsiI PspOMI PspXI PsrI PvuI PvuII RsrII
SacI SacII SalI SanDI SapI SbfI ScaI SfiI
SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI
SspI SwaI TstI Tth111I XbaI XhoI XmaI XmnI