Restriction map for
pFN21AE9565
(Linear) (Six-base) MAPSORT of: KIEE9565.seq Check: 4511 from: 1 to: 1179
KIEE9565.orf
With 182 enzymes: SgfI *
July 3, 2012 19:02 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 93 1179
Size: 93 1086
AleI CACnn'nnGTG
Cuts at: 0 913 1179
Size: 913 266
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 841 1179
Size: 841 338
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 763 795 1179
Size: 763 32 384
Fragments arranged by size:
763 384 32
AlwNI CAG_nnn'CTG
Cuts at: 0 538 1179
Size: 538 641
ApaI G_GGCC'C
Cuts at: 0 730 1179
Size: 730 449
ApaLI G'TGCA_C
Cuts at: 0 408 1179
Size: 408 771
ApoI r'AATT_y
Cuts at: 0 718 1179
Size: 718 461
AvaI C'yCGr_G
Cuts at: 0 767 1179
Size: 767 412
BanI G'GyrC_C
Cuts at: 0 891 1179
Size: 891 288
BanII G_rGCy'C
Cuts at: 0 84 730 776 1179
Size: 84 646 46 403
Fragments arranged by size:
646 403 84 46
BbeI G_GCGC'C
Cuts at: 0 895 1179
Size: 895 284
BbsI GAAGACnn'nnnn_
Cuts at: 0 847 1179
Size: 847 332
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 702 736 1179
Size: 702 34 443
Fragments arranged by size:
702 443 34
BclI T'GATC_A
Cuts at: 0 1007 1179
Size: 1007 172
BglI GCCn_nnn'nGGC
Cuts at: 0 310 466 1179
Size: 310 156 713
Fragments arranged by size:
713 310 156
BglII A'GATC_T
Cuts at: 0 641 656 1179
Size: 641 15 523
Fragments arranged by size:
641 523 15
Bme1580I G_kGCm'C
Cuts at: 0 412 730 1179
Size: 412 318 449
Fragments arranged by size:
449 412 318
BmgBI CAC'GTC
Cuts at: 0 402 1179
Size: 402 777
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1111 1179
Size: 1111 68
BsaI GGTCTCn'nnnn_
Cuts at: 0 504 1179
Size: 504 675
BsaAI yAC'GTr
Cuts at: 0 879 1179
Size: 879 300
BsaBI GATnn'nnATC
Cuts at: 0 580 1179
Size: 580 599
BsaHI Gr'CG_yC
Cuts at: 0 892 1179
Size: 892 287
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 763 793 1179
Size: 763 30 386
Fragments arranged by size:
763 386 30
BseYI C'CCAG_C
Cuts at: 0 887 1179
Size: 887 292
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1133 1179
Size: 1133 46
BsiHKAI G_wGCw'C
Cuts at: 0 68 395 412 776 857 1179
Size: 68 327 17 364 81 322
Fragments arranged by size:
364 327 322 81 68 17
BsmBI CGTCTCn'nnnn_
Cuts at: 0 601 1179
Size: 601 578
Bsp1286I G_dGCh'C
Cuts at: 0 68 84 395 412 730 776 857 1179
Size: 68 16 311 17 318 46 81 322
Fragments arranged by size:
322 318 311 81 68 46 17 16
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 302 434 1179
Size: 302 132 745
Fragments arranged by size:
745 302 132
BstEII G'GTnAC_C
Cuts at: 0 735 1179
Size: 735 444
BstYI r'GATC_y
Cuts at: 0 641 656 761 812 1179
Size: 641 15 105 51 367
Fragments arranged by size:
641 367 105 51 15
BtgI C'CryG_G
Cuts at: 0 11 514 731 1179
Size: 11 503 217 448
Fragments arranged by size:
503 448 217 11
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 285 456 972 1179
Size: 285 171 516 207
Fragments arranged by size:
516 285 207 171
BtsI GCAGTG_nn'
Cuts at: 0 962 1179
Size: 962 217
DraIII CAC_nnn'GTG
Cuts at: 0 1019 1179
Size: 1019 160
EaeI y'GGCC_r
Cuts at: 0 311 332 396 467 651 1179
Size: 311 21 64 71 184 528
Fragments arranged by size:
528 311 184 71 64 21
EarI CTCTTCn'nnn_
Cuts at: 0 1148 1179
Size: 1148 31
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 579 1179
Size: 579 600
EcoICRI GAG'CTC
Cuts at: 0 774 1179
Size: 774 405
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 93 1179
Size: 93 1086
EcoO109I rG'GnC_Cy
Cuts at: 0 727 1179
Size: 727 452
EcoRI G'AATT_C
Cuts at: 0 718 1179
Size: 718 461
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 711 1179
Size: 711 468
FspI TGC'GCA
Cuts at: 0 280 430 1179
Size: 280 150 749
Fragments arranged by size:
749 280 150
HaeII r_GCGC'y
Cuts at: 0 895 1179
Size: 895 284
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 999 1031 1179
Size: 999 32 148
Fragments arranged by size:
999 148 32
HincII GTy'rAC
Cuts at: 0 420 1179
Size: 420 759
KasI G'GCGC_C
Cuts at: 0 891 1179
Size: 891 288
MfeI C'AATT_G
Cuts at: 0 1133 1179
Size: 1133 46
MscI TGG'CCA
Cuts at: 0 313 334 469 1179
Size: 313 21 135 710
Fragments arranged by size:
710 313 135 21
MslI CAynn'nnrTG
Cuts at: 0 196 913 991 1179
Size: 196 717 78 188
Fragments arranged by size:
717 196 188 78
MspA1I CmG'CkG
Cuts at: 0 507 1179
Size: 507 672
NarI GG'CG_CC
Cuts at: 0 892 1179
Size: 892 287
NcoI C'CATG_G
Cuts at: 0 11 1179
Size: 11 1168
PfoI T'CCnGG_A
Cuts at: 0 163 1179
Size: 163 1016
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 763 795 1179
Size: 763 32 384
Fragments arranged by size:
763 384 32
PspOMI G'GGCC_C
Cuts at: 0 726 1179
Size: 726 453
SacI G_AGCT'C
Cuts at: 0 776 1179
Size: 776 403
ScaI AGT'ACT
Cuts at: 0 1054 1179
Size: 1054 125
SfcI C'TryA_G
Cuts at: 0 114 1179
Size: 114 1065
SfoI GGC'GCC
Cuts at: 0 893 1179
Size: 893 286
SmaI CCC'GGG
Cuts at: 0 769 1179
Size: 769 410
SmlI C'TyrA_G
Cuts at: 0 1126 1179
Size: 1126 53
StyI C'CwwG_G
Cuts at: 0 11 551 1179
Size: 11 540 628
Fragments arranged by size:
628 540 11
TatI w'GTAC_w
Cuts at: 0 1052 1179
Size: 1052 127
Tth111I GACn'n_nGTC
Cuts at: 0 564 1045 1179
Size: 564 481 134
Fragments arranged by size:
564 481 134
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 18 462 535 1116 1155 1179
Size: 18 444 73 581 39 24
Fragments arranged by size:
581 444 73 39 24 18
XmaI C'CCGG_G
Cuts at: 0 767 1179
Size: 767 412
Enzymes that do cut:
AcuI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AvaI BanI BanII BbeI BbsI BcgI BclI BglI
BglII Bme1580I BmgBI BpuEI BsaI BsaAI BsaBI BsaHI
BsaXI BseYI BsgI BsiHKAI BsmBI Bsp1286I BspMI BstEII
BstYI BtgI BtgZI BtsI DraIII EaeI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII KasI MfeI MscI MslI MspA1I NarI NcoI
PfoI PpiI PspOMI SacI ScaI SfcI SfoI SmaI
SmlI StyI TatI Tth111I XcmI XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AscI AseI AsiSI AvrII BaeI BamHI
BbvCI BciVI BfrBI BlpI BmrI BmtI BplI BpmI
Bpu10I BsaWI BseRI BsiEI BsiWI BsmI BspEI BspHI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstXI BstZ17I Bsu36I ClaI CspCI DraI DrdI EagI
EcoNI EcoRV FseI FspAI HindIII HpaI KpnI MluI
MmeI NaeI NdeI NgoMIV NheI NotI NruI NsiI
NspI PacI PasI PciI PflMI PmeI PmlI PpuMI
PshAI PsiI PspXI PsrI PstI PvuI PvuII RsrII
SacII SalI SanDI SapI SbfI SexAI SfiI SgfI
SgrAI SnaBI SpeI SphI SrfI SspI StuI SwaI
TaqII TstI XbaI XhoI XmnI ZraI