Restriction map for
pFN21AE9562
(Linear) (Six-base) MAPSORT of: KIEE9562.seq Check: 3614 from: 1 to: 1086
KIEE9562.orf
With 182 enzymes: SgfI *
December 27, 2011 16:42 ..
AatII G_ACGT'C
Cuts at: 0 164 1086
Size: 164 922
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 667 745 1086
Size: 667 78 341
Fragments arranged by size:
667 341 78
AfeI AGC'GCT
Cuts at: 0 125 1086
Size: 125 961
AleI CACnn'nnGTG
Cuts at: 0 154 505 1086
Size: 154 351 581
Fragments arranged by size:
581 351 154
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 688 1086
Size: 688 398
ApaI G_GGCC'C
Cuts at: 0 321 1086
Size: 321 765
AvaI C'yCGr_G
Cuts at: 0 57 1086
Size: 57 1029
BamHI G'GATC_C
Cuts at: 0 962 1086
Size: 962 124
BanII G_rGCy'C
Cuts at: 0 321 795 929 1042 1086
Size: 321 474 134 113 44
Fragments arranged by size:
474 321 134 113 44
BbsI GAAGACnn'nnnn_
Cuts at: 0 375 1086
Size: 375 711
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 795 829 1086
Size: 795 34 257
Fragments arranged by size:
795 257 34
Bme1580I G_kGCm'C
Cuts at: 0 321 1086
Size: 321 765
BmrI ACTGGGnnnn_n'
Cuts at: 0 588 1086
Size: 588 498
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 30 1061 1086
Size: 30 1031 25
Fragments arranged by size:
1031 30 25
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 75 104 562 1086
Size: 75 29 458 524
Fragments arranged by size:
524 458 75 29
Bpu10I CC'TnA_GC
Cuts at: 0 196 364 1086
Size: 196 168 722
Fragments arranged by size:
722 196 168
BsaHI Gr'CG_yC
Cuts at: 0 161 1086
Size: 161 925
BsaWI w'CCGG_w
Cuts at: 0 212 1086
Size: 212 874
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 343 487 619 1086
Size: 343 144 132 467
Fragments arranged by size:
467 343 144 132
BseYI C'CCAG_C
Cuts at: 0 314 730 788 1041 1086
Size: 314 416 58 253 45
Fragments arranged by size:
416 314 253 58 45
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 460 552 1084 1086
Size: 460 92 532 2
Fragments arranged by size:
532 460 92 2
BsiHKAI G_wGCw'C
Cuts at: 0 140 197 335 1086
Size: 140 57 138 751
Fragments arranged by size:
751 140 138 57
BsmI GAATG_Cn'
Cuts at: 0 915 995 1086
Size: 915 80 91
Fragments arranged by size:
915 91 80
Bsp1286I G_dGCh'C
Cuts at: 0 140 197 321 335 795 929 1042 1086
Size: 140 57 124 14 460 134 113 44
Fragments arranged by size:
460 140 134 124 113 57 44 14
BspEI T'CCGG_A
Cuts at: 0 212 1086
Size: 212 874
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 211 668 1086
Size: 211 457 418
Fragments arranged by size:
457 418 211
BsrFI r'CCGG_y
Cuts at: 0 455 1086
Size: 455 631
BssHII G'CGCG_C
Cuts at: 0 80 1086
Size: 80 1006
BssSI C'ACGA_G
Cuts at: 0 292 1086
Size: 292 794
BstAPI GCAn_nnn'nTGC
Cuts at: 0 719 1086
Size: 719 367
BstXI CCAn_nnnn'nTGG
Cuts at: 0 156 762 1086
Size: 156 606 324
Fragments arranged by size:
606 324 156
BstYI r'GATC_y
Cuts at: 0 851 962 1086
Size: 851 111 124
Fragments arranged by size:
851 124 111
Bsu36I CC'TnA_GG
Cuts at: 0 857 1086
Size: 857 229
BtgI C'CryG_G
Cuts at: 0 131 155 503 985 1086
Size: 131 24 348 482 101
Fragments arranged by size:
482 348 131 101 24
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 384 1086
Size: 384 702
BtsI GCAGTG_nn'
Cuts at: 0 56 333 392 614 990 1086
Size: 56 277 59 222 376 96
Fragments arranged by size:
376 277 222 96 59 56
EaeI y'GGCC_r
Cuts at: 0 296 821 1086
Size: 296 525 265
Fragments arranged by size:
525 296 265
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 75 104 562 667 745 1086
Size: 75 29 458 105 78 341
Fragments arranged by size:
458 341 105 78 75 29
EcoNI CCTnn'n_nnAGG
Cuts at: 0 325 1086
Size: 325 761
EcoO109I rG'GnC_Cy
Cuts at: 0 95 318 536 1086
Size: 95 223 218 550
Fragments arranged by size:
550 223 218 95
FspI TGC'GCA
Cuts at: 0 664 1086
Size: 664 422
HaeII r_GCGC'y
Cuts at: 0 127 350 462 1086
Size: 127 223 112 624
Fragments arranged by size:
624 223 127 112
MscI TGG'CCA
Cuts at: 0 298 823 1086
Size: 298 525 263
Fragments arranged by size:
525 298 263
MslI CAynn'nnrTG
Cuts at: 0 154 505 670 760 1060 1086
Size: 154 351 165 90 300 26
Fragments arranged by size:
351 300 165 154 90 26
MspA1I CmG'CkG
Cuts at: 0 133 902 1086
Size: 133 769 184
Fragments arranged by size:
769 184 133
PasI CC'CwG_GG
Cuts at: 0 110 321 1086
Size: 110 211 765
Fragments arranged by size:
765 211 110
PflMI CCAn_nnn'nTGG
Cuts at: 0 435 788 840 1086
Size: 435 353 52 246
Fragments arranged by size:
435 353 246 52
PfoI T'CCnGG_A
Cuts at: 0 91 1086
Size: 91 995
PpuMI rG'GwC_Cy
Cuts at: 0 95 536 1086
Size: 95 441 550
Fragments arranged by size:
550 441 95
PspOMI G'GGCC_C
Cuts at: 0 317 1086
Size: 317 769
PstI C_TGCA'G
Cuts at: 0 1037 1086
Size: 1037 49
PvuII CAG'CTG
Cuts at: 0 902 1086
Size: 902 184
SacII CC_GC'GG
Cuts at: 0 134 1086
Size: 134 952
SanDI GG'GwC_CC
Cuts at: 0 536 1086
Size: 536 550
SexAI A'CCwGG_T
Cuts at: 0 257 1086
Size: 257 829
SfcI C'TryA_G
Cuts at: 0 1033 1086
Size: 1033 53
SmaI CCC'GGG
Cuts at: 0 59 1086
Size: 59 1027
SrfI GCCC'GGGC
Cuts at: 0 59 1086
Size: 59 1027
StyI C'CwwG_G
Cuts at: 0 1010 1086
Size: 1010 76
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 102 607 660 1065 1086
Size: 102 505 53 405 21
Fragments arranged by size:
505 405 102 53 21
TatI w'GTAC_w
Cuts at: 0 596 1086
Size: 596 490
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 528 1086
Size: 528 558
XmaI C'CCGG_G
Cuts at: 0 57 1086
Size: 57 1029
ZraI GAC'GTC
Cuts at: 0 162 1086
Size: 162 924
Enzymes that do cut:
AatII AcuI AfeI AleI AlfI ApaI AvaI BamHI
BanII BbsI BcgI Bme1580I BmrI BplI BpmI Bpu10I
BsaHI BsaWI BseRI BseYI BsgI BsiHKAI BsmI Bsp1286I
BspEI BspMI BsrFI BssHII BssSI BstAPI BstXI BstYI
Bsu36I BtgI BtgZI BtsI EaeI Eco57MI EcoNI EcoO109I
FspI HaeII MscI MslI MspA1I PasI PflMI PfoI
PpuMI PspOMI PstI PvuII SacII SanDI SexAI SfcI
SmaI SrfI StyI TaqII TatI XcmI XmaI ZraI
Enzymes that do not cut:
AarI AccI Acc65I AclI AflII AflIII AgeI AhdI
AloI AlwNI ApaLI ApoI AscI AseI AsiSI AvrII
BaeI BanI BbeI BbvCI BciVI BclI BfrBI BglI
BglII BlpI BmgBI BmtI BpuEI BsaI BsaAI BsaBI
BsaXI BsiEI BsiWI BsmBI BspHI BsrBI BsrDI BsrGI
BstBI BstEII BstZ17I ClaI CspCI DraI DraIII DrdI
EagI EarI EciI EcoICRI EcoRI EcoRV FalI FseI
FspAI Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI NaeI NarI NcoI NdeI NgoMIV NheI
NotI NruI NsiI NspI PacI PciI PmeI PmlI
PpiI PshAI PsiI PspXI PsrI PvuI RsrII SacI
SalI SapI SbfI ScaI SfiI SfoI SgfI SgrAI
SmlI SnaBI SpeI SphI SspI StuI SwaI TstI
Tth111I XbaI XhoI XmnI