Restriction map for
pFN21AE9560
(Linear) (Six-base) MAPSORT of: KIEE9560.seq Check: 2441 from: 1 to: 1155
KIEE9560.orf
With 182 enzymes: SgfI *
March 22, 2012 18:31 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 416 745 1155
Size: 416 329 410
Fragments arranged by size:
416 410 329
AccI GT'mk_AC
Cuts at: 0 74 1155
Size: 74 1081
AfeI AGC'GCT
Cuts at: 0 421 1155
Size: 421 734
AflIII A'CryG_T
Cuts at: 0 476 1025 1155
Size: 476 549 130
Fragments arranged by size:
549 476 130
AleI CACnn'nnGTG
Cuts at: 0 410 1155
Size: 410 745
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 919 1155
Size: 919 236
AlwNI CAG_nnn'CTG
Cuts at: 0 941 1155
Size: 941 214
ApaI G_GGCC'C
Cuts at: 0 552 1155
Size: 552 603
ApaLI G'TGCA_C
Cuts at: 0 535 1155
Size: 535 620
AvaI C'yCGr_G
Cuts at: 0 927 1155
Size: 927 228
BamHI G'GATC_C
Cuts at: 0 1005 1155
Size: 1005 150
BanI G'GyrC_C
Cuts at: 0 55 222 237 315 569 958 1132 1155
Size: 55 167 15 78 254 389 174 23
Fragments arranged by size:
389 254 174 167 78 55 23 15
BanII G_rGCy'C
Cuts at: 0 188 552 934 1064 1155
Size: 188 364 382 130 91
Fragments arranged by size:
382 364 188 130 91
BbeI G_GCGC'C
Cuts at: 0 573 1155
Size: 573 582
BbvCI CC'TCA_GC
Cuts at: 0 844 1155
Size: 844 311
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 301 335 1155
Size: 301 34 820
Fragments arranged by size:
820 301 34
BglI GCCn_nnn'nGGC
Cuts at: 0 50 231 313 619 1099 1155
Size: 50 181 82 306 480 56
Fragments arranged by size:
480 306 181 82 56 50
BlpI GC'TnA_GC
Cuts at: 0 982 1155
Size: 982 173
Bme1580I G_kGCm'C
Cuts at: 0 207 227 242 539 552 893 971 1135
Size: 207 20 15 297 13 341 78 164
Cuts at: 1135 1155
Size: 20
Fragments arranged by size:
341 297 207 164 78 20 20 15
13
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 202 1135 1155
Size: 202 933 20
Fragments arranged by size:
933 202 20
Bpu10I CC'TnA_GC
Cuts at: 0 844 1155
Size: 844 311
BsaAI yAC'GTr
Cuts at: 0 966 1026 1155
Size: 966 60 129
Fragments arranged by size:
966 129 60
BsaHI Gr'CG_yC
Cuts at: 0 570 1155
Size: 570 585
BsaWI w'CCGG_w
Cuts at: 0 94 1155
Size: 94 1061
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1131 1155
Size: 1131 24
BseYI C'CCAG_C
Cuts at: 0 33 67 218 892 934 970 1155
Size: 33 34 151 674 42 36 185
Fragments arranged by size:
674 185 151 42 36 34 33
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 787 1155
Size: 787 368
BsiEI CG_ry'CG
Cuts at: 0 461 614 672 825 1034 1075 1155
Size: 461 153 58 153 209 41 80
Fragments arranged by size:
461 209 153 153 80 58 41
BsiHKAI G_wGCw'C
Cuts at: 0 94 188 539 680 988 1155
Size: 94 94 351 141 308 167
Fragments arranged by size:
351 308 167 141 94 94
Bsp1286I G_dGCh'C
Cuts at: 0 94 188 207 227 242 539 552 680
Size: 94 94 19 20 15 297 13 128
Cuts at: 680 893 934 971 988 1064 1135 1155
Size: 213 41 37 17 76 71 20
Fragments arranged by size:
297 213 128 94 94 76 71 41
37 20 20 19 17 15 13
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 416 745 908 1155
Size: 416 329 163 247
Fragments arranged by size:
416 329 247 163
BsrBI CCG'CTC
Cuts at: 0 86 1155
Size: 86 1069
BsrFI r'CCGG_y
Cuts at: 0 276 566 726 824 1155
Size: 276 290 160 98 331
Fragments arranged by size:
331 290 276 160 98
BssHII G'CGCG_C
Cuts at: 0 661 1155
Size: 661 494
BstAPI GCAn_nnn'nTGC
Cuts at: 0 543 1155
Size: 543 612
BstXI CCAn_nnnn'nTGG
Cuts at: 0 234 288 412 1155
Size: 234 54 124 743
Fragments arranged by size:
743 234 124 54
BstYI r'GATC_y
Cuts at: 0 1005 1155
Size: 1005 150
BtgI C'CryG_G
Cuts at: 0 138 391 455 875 1088 1155
Size: 138 253 64 420 213 67
Fragments arranged by size:
420 253 213 138 67 64
BtsI GCAGTG_nn'
Cuts at: 0 205 622 634 863 1155
Size: 205 417 12 229 292
Fragments arranged by size:
417 292 229 205 12
EaeI y'GGCC_r
Cuts at: 0 278 305 414 452 458 500 564 611
Size: 278 27 109 38 6 42 64 47
Cuts at: 611 773 815 822 834 1031 1155
Size: 162 42 7 12 197 124
Fragments arranged by size:
278 197 162 124 109 64 47 42
42 38 27 12 7 6
EagI C'GGCC_G
Cuts at: 0 458 611 822 1031 1155
Size: 458 153 211 209 124
Fragments arranged by size:
458 211 209 153 124
EarI CTCTTCn'nnn_
Cuts at: 0 1069 1155
Size: 1069 86
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 861 1155
Size: 861 294
EcoICRI GAG'CTC
Cuts at: 0 186 1155
Size: 186 969
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 202 1135 1155
Size: 202 933 20
Fragments arranged by size:
933 202 20
HaeII r_GCGC'y
Cuts at: 0 295 385 423 573 748 831 941 982
Size: 295 90 38 150 175 83 110 41
Cuts at: 982 1155
Size: 173
Fragments arranged by size:
295 175 173 150 110 90 83 41
38
KasI G'GCGC_C
Cuts at: 0 569 1155
Size: 569 586
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1092 1155
Size: 1092 63
MscI TGG'CCA
Cuts at: 0 307 416 454 775 1155
Size: 307 109 38 321 380
Fragments arranged by size:
380 321 307 109 38
MslI CAynn'nnrTG
Cuts at: 0 410 479 1155
Size: 410 69 676
Fragments arranged by size:
676 410 69
MspA1I CmG'CkG
Cuts at: 0 140 393 543 642 821 998 1155
Size: 140 253 150 99 179 177 157
Fragments arranged by size:
253 179 177 157 150 140 99
NaeI GCC'GGC
Cuts at: 0 278 568 826 1155
Size: 278 290 258 329
Fragments arranged by size:
329 290 278 258
NarI GG'CG_CC
Cuts at: 0 570 1155
Size: 570 585
NgoMIV G'CCGG_C
Cuts at: 0 276 566 824 1155
Size: 276 290 258 331
Fragments arranged by size:
331 290 276 258
NotI GC'GGCC_GC
Cuts at: 0 611 1155
Size: 611 544
NruI TCG'CGA
Cuts at: 0 355 1155
Size: 355 800
NspI r_CATG'y
Cuts at: 0 480 1155
Size: 480 675
PciI A'CATG_T
Cuts at: 0 476 1155
Size: 476 679
PflMI CCAn_nnn'nTGG
Cuts at: 0 941 1155
Size: 941 214
PfoI T'CCnGG_A
Cuts at: 0 188 1155
Size: 188 967
PspOMI G'GGCC_C
Cuts at: 0 548 1155
Size: 548 607
PstI C_TGCA'G
Cuts at: 0 641 1155
Size: 641 514
PvuI CG_AT'CG
Cuts at: 0 672 1155
Size: 672 483
PvuII CAG'CTG
Cuts at: 0 998 1155
Size: 998 157
SacI G_AGCT'C
Cuts at: 0 188 1155
Size: 188 967
SacII CC_GC'GG
Cuts at: 0 141 394 1155
Size: 141 253 761
Fragments arranged by size:
761 253 141
SapI GCTCTTCn'nnn_
Cuts at: 0 1069 1155
Size: 1069 86
SfcI C'TryA_G
Cuts at: 0 637 1155
Size: 637 518
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 1099 1155
Size: 1099 56
SfoI GGC'GCC
Cuts at: 0 571 1155
Size: 571 584
StuI AGG'CCT
Cuts at: 0 712 1102 1155
Size: 712 390 53
Fragments arranged by size:
712 390 53
StyI C'CwwG_G
Cuts at: 0 579 1097 1155
Size: 579 518 58
Fragments arranged by size:
579 518 58
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 73 210 288 1150 1155
Size: 73 137 78 862 5
Fragments arranged by size:
862 137 78 73 5
Enzymes that do cut:
AarI AccI AfeI AflIII AleI AlfI AlwNI ApaI
ApaLI AvaI BamHI BanI BanII BbeI BbvCI BcgI
BglI BlpI Bme1580I BpmI Bpu10I BsaAI BsaHI BsaWI
BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspMI BsrBI
BsrFI BssHII BstAPI BstXI BstYI BtgI BtsI EaeI
EagI EarI EciI EcoICRI Eco57MI HaeII KasI MmeI
MscI MslI MspA1I NaeI NarI NgoMIV NotI NruI
NspI PciI PflMI PfoI PspOMI PstI PvuI PvuII
SacI SacII SapI SfcI SfiI SfoI StuI StyI
TaqII
Enzymes that do not cut:
AatII Acc65I AclI AcuI AflII AgeI AhdI AloI
ApoI AscI AseI AsiSI AvrII BaeI BbsI BciVI
BclI BfrBI BglII BmgBI BmrI BmtI BplI BpuEI
BsaI BsaBI BsaXI BsiWI BsmI BsmBI BspEI BspHI
BsrDI BsrGI BssSI BstBI BstEII BstZ17I Bsu36I BtgZI
ClaI CspCI DraI DraIII DrdI EcoNI EcoO109I EcoRI
EcoRV FalI FseI FspI FspAI Hin4I HincII HindIII
HpaI KpnI MfeI MluI NcoI NdeI NheI NsiI
PacI PasI PmeI PmlI PpiI PpuMI PshAI PsiI
PspXI PsrI RsrII SalI SanDI SbfI ScaI SexAI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI
SspI SwaI TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI