Restriction map for
pFN21AE9558
(Linear) (Six-base) MAPSORT of: KIEE9558.seq Check: 6407 from: 1 to: 1431
KIEE9558.orf
With 182 enzymes: SgfI *
July 3, 2012 19:02 ..
Acc65I G'GTAC_C
Cuts at: 0 248 1431
Size: 248 1183
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 774 1431
Size: 774 657
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1357 1431
Size: 1357 74
AlwNI CAG_nnn'CTG
Cuts at: 0 641 1179 1431
Size: 641 538 252
Fragments arranged by size:
641 538 252
ApaI G_GGCC'C
Cuts at: 0 305 465 1431
Size: 305 160 966
Fragments arranged by size:
966 305 160
AvaI C'yCGr_G
Cuts at: 0 329 775 1431
Size: 329 446 656
Fragments arranged by size:
656 446 329
BamHI G'GATC_C
Cuts at: 0 914 1431
Size: 914 517
BanI G'GyrC_C
Cuts at: 0 248 260 817 1123 1244 1253 1431
Size: 248 12 557 306 121 9 178
Fragments arranged by size:
557 306 248 178 121 12 9
BanII G_rGCy'C
Cuts at: 0 305 465 1426 1431
Size: 305 160 961 5
Fragments arranged by size:
961 305 160 5
BbeI G_GCGC'C
Cuts at: 0 1257 1431
Size: 1257 174
BbsI GAAGACnn'nnnn_
Cuts at: 0 924 1431
Size: 924 507
BbvCI CC'TCA_GC
Cuts at: 0 496 1162 1431
Size: 496 666 269
Fragments arranged by size:
666 496 269
BclI T'GATC_A
Cuts at: 0 947 1431
Size: 947 484
Bme1580I G_kGCm'C
Cuts at: 0 305 465 822 1005 1126 1431
Size: 305 160 357 183 121 305
Fragments arranged by size:
357 305 305 183 160 121
BmrI ACTGGGnnnn_n'
Cuts at: 0 351 1431
Size: 351 1080
Bpu10I CC'TnA_GC
Cuts at: 0 77 139 496 592 1162 1431
Size: 77 62 357 96 570 269
Fragments arranged by size:
570 357 269 96 77 62
BsaI GGTCTCn'nnnn_
Cuts at: 0 1378 1431
Size: 1378 53
BsaHI Gr'CG_yC
Cuts at: 0 1076 1254 1431
Size: 1076 178 177
Fragments arranged by size:
1076 178 177
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 154 184 724 754 1207 1237 1344 1374
Size: 154 30 540 30 453 30 107 30
Cuts at: 1374 1431
Size: 57
Fragments arranged by size:
540 453 154 107 57 30 30 30
30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 391 460 795 1291 1431
Size: 391 69 335 496 140
Fragments arranged by size:
496 391 335 140 69
BseYI C'CCAG_C
Cuts at: 0 756 812 1071 1330 1431
Size: 756 56 259 259 101
Fragments arranged by size:
756 259 259 101 56
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 490 495 730 1240 1431
Size: 490 5 235 510 191
Fragments arranged by size:
510 490 235 191 5
BsiHKAI G_wGCw'C
Cuts at: 0 1115 1431
Size: 1115 316
Bsp1286I G_dGCh'C
Cuts at: 0 305 465 822 1005 1115 1126 1426 1431
Size: 305 160 357 183 110 11 300 5
Fragments arranged by size:
357 305 300 183 160 110 11 5
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 260 752 761 1431
Size: 260 492 9 670
Fragments arranged by size:
670 492 260 9
BsrBI CCG'CTC
Cuts at: 0 76 658 1281 1431
Size: 76 582 623 150
Fragments arranged by size:
623 582 150 76
BsrDI GCAATG_nn'
Cuts at: 0 525 897 1431
Size: 525 372 534
Fragments arranged by size:
534 525 372
BsrGI T'GTAC_A
Cuts at: 0 410 431 1431
Size: 410 21 1000
Fragments arranged by size:
1000 410 21
BssSI C'ACGA_G
Cuts at: 0 985 1431
Size: 985 446
BstAPI GCAn_nnn'nTGC
Cuts at: 0 753 1431
Size: 753 678
BstEII G'GTnAC_C
Cuts at: 0 118 1431
Size: 118 1313
BstXI CCAn_nnnn'nTGG
Cuts at: 0 165 370 616 1431
Size: 165 205 246 815
Fragments arranged by size:
815 246 205 165
BstYI r'GATC_y
Cuts at: 0 914 1431
Size: 914 517
BtgI C'CryG_G
Cuts at: 0 158 224 680 1320 1431
Size: 158 66 456 640 111
Fragments arranged by size:
640 456 158 111 66
BtsI GCAGTG_nn'
Cuts at: 0 507 854 1431
Size: 507 347 577
Fragments arranged by size:
577 507 347
DraIII CAC_nnn'GTG
Cuts at: 0 270 1086 1431
Size: 270 816 345
Fragments arranged by size:
816 345 270
DrdI GACnn_nn'nnGTC
Cuts at: 0 631 1431
Size: 631 800
EaeI y'GGCC_r
Cuts at: 0 202 227 401 479 677 707 764 938
Size: 202 25 174 78 198 30 57 174
Cuts at: 938 989 1040 1317 1431
Size: 51 51 277 114
Fragments arranged by size:
277 202 198 174 174 114 78 57
51 51 30 25
EarI CTCTTCn'nnn_
Cuts at: 0 1150 1162 1431
Size: 1150 12 269
Fragments arranged by size:
1150 269 12
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1125 1431
Size: 1125 306
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 774 1431
Size: 774 657
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1210 1431
Size: 1210 221
EcoO109I rG'GnC_Cy
Cuts at: 0 66 461 462 492 596 735 1176 1301
Size: 66 395 1 30 104 139 441 125
Cuts at: 1301 1431
Size: 130
Fragments arranged by size:
441 395 139 130 125 104 66 30
1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1164 1431
Size: 1164 267
HaeII r_GCGC'y
Cuts at: 0 1257 1431
Size: 1257 174
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1207 1239 1431
Size: 1207 32 192
Fragments arranged by size:
1207 192 32
KasI G'GCGC_C
Cuts at: 0 1253 1431
Size: 1253 178
KpnI G_GTAC'C
Cuts at: 0 252 1431
Size: 252 1179
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1205 1431
Size: 1205 226
MscI TGG'CCA
Cuts at: 0 403 481 709 766 940 991 1042 1431
Size: 403 78 228 57 174 51 51 389
Fragments arranged by size:
403 389 228 174 78 57 51 51
MspA1I CmG'CkG
Cuts at: 0 682 981 1250 1431
Size: 682 299 269 181
Fragments arranged by size:
682 299 269 181
NarI GG'CG_CC
Cuts at: 0 1254 1431
Size: 1254 177
PasI CC'CwG_GG
Cuts at: 0 456 842 1119 1431
Size: 456 386 277 312
Fragments arranged by size:
456 386 312 277
PflMI CCAn_nnn'nTGG
Cuts at: 0 1086 1431
Size: 1086 345
PpuMI rG'GwC_Cy
Cuts at: 0 66 596 1431
Size: 66 530 835
Fragments arranged by size:
835 530 66
PshAI GACnn'nnGTC
Cuts at: 0 125 1431
Size: 125 1306
PspOMI G'GGCC_C
Cuts at: 0 301 461 1431
Size: 301 160 970
Fragments arranged by size:
970 301 160
PstI C_TGCA'G
Cuts at: 0 1346 1431
Size: 1346 85
PvuII CAG'CTG
Cuts at: 0 981 1431
Size: 981 450
SacII CC_GC'GG
Cuts at: 0 683 1431
Size: 683 748
SapI GCTCTTCn'nnn_
Cuts at: 0 1150 1431
Size: 1150 281
SfcI C'TryA_G
Cuts at: 0 444 603 1342 1431
Size: 444 159 739 89
Fragments arranged by size:
739 444 159 89
SfoI GGC'GCC
Cuts at: 0 1255 1431
Size: 1255 176
SmaI CCC'GGG
Cuts at: 0 331 1431
Size: 331 1100
StyI C'CwwG_G
Cuts at: 0 256 340 476 1391 1431
Size: 256 84 136 915 40
Fragments arranged by size:
915 256 136 84 40
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 306 1431
Size: 306 1125
TatI w'GTAC_w
Cuts at: 0 410 431 1431
Size: 410 21 1000
Fragments arranged by size:
1000 410 21
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 154 186 1344 1376 1431
Size: 154 32 1158 32 55
Fragments arranged by size:
1158 154 55 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 570 1431
Size: 570 861
XmaI C'CCGG_G
Cuts at: 0 329 1431
Size: 329 1102
Enzymes that do cut:
Acc65I AcuI AlfI AlwNI ApaI AvaI BamHI BanI
BanII BbeI BbsI BbvCI BclI Bme1580I BmrI Bpu10I
BsaI BsaHI BsaXI BseRI BseYI BsgI BsiHKAI Bsp1286I
BspMI BsrBI BsrDI BsrGI BssSI BstAPI BstEII BstXI
BstYI BtgI BtsI DraIII DrdI EaeI EarI EciI
Eco57MI EcoNI EcoO109I FalI HaeII Hin4I KasI KpnI
MmeI MscI MspA1I NarI PasI PflMI PpuMI PshAI
PspOMI PstI PvuII SacII SapI SfcI SfoI SmaI
StyI TaqII TatI TstI XcmI XmaI
Enzymes that do not cut:
AarI AatII AccI AclI AfeI AflII AflIII AgeI
AhdI AleI AloI ApaLI ApoI AscI AseI AsiSI
AvrII BaeI BcgI BciVI BfrBI BglI BglII BlpI
BmgBI BmtI BplI BpmI BpuEI BsaAI BsaBI BsaWI
BsiEI BsiWI BsmI BsmBI BspEI BspHI BsrFI BssHII
BstBI BstZ17I Bsu36I BtgZI ClaI CspCI DraI EagI
EcoICRI EcoRI EcoRV FseI FspI FspAI HincII HindIII
HpaI MfeI MluI MslI NaeI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspI PacI PciI PfoI
PmeI PmlI PpiI PsiI PspXI PsrI PvuI RsrII
SacI SalI SanDI SbfI ScaI SexAI SfiI SgfI
SgrAI SmlI SnaBI SpeI SphI SrfI SspI StuI
SwaI Tth111I XbaI XhoI XmnI ZraI