Restriction map for
pFN21AE9555
(Linear) (Six-base) MAPSORT of: KIEE9555.seq Check: 9940 from: 1 to: 3540
KIEE9555.orf
With 182 enzymes: SgfI *
December 27, 2011 16:42 ..
AccI GT'mk_AC
Cuts at: 0 2186 3540
Size: 2186 1354
AclI AA'CG_TT
Cuts at: 0 1448 3540
Size: 1448 2092
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 341 563 802 1045 1921 3393 3540
Size: 341 222 239 243 876 1472 147
Fragments arranged by size:
1472 876 341 243 239 222 147
AfeI AGC'GCT
Cuts at: 0 2814 3540
Size: 2814 726
AflII C'TTAA_G
Cuts at: 0 2625 3540
Size: 2625 915
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2470 3540
Size: 2470 1070
AlwNI CAG_nnn'CTG
Cuts at: 0 2688 2700 3255 3540
Size: 2688 12 555 285
Fragments arranged by size:
2688 555 285 12
ApaI G_GGCC'C
Cuts at: 0 2658 2873 3540
Size: 2658 215 667
Fragments arranged by size:
2658 667 215
ApaLI G'TGCA_C
Cuts at: 0 56 649 3540
Size: 56 593 2891
Fragments arranged by size:
2891 593 56
ApoI r'AATT_y
Cuts at: 0 477 892 1069 1407 3540
Size: 477 415 177 338 2133
Fragments arranged by size:
2133 477 415 338 177
AvaI C'yCGr_G
Cuts at: 0 812 2872 3323 3346 3426 3540
Size: 812 2060 451 23 80 114
Fragments arranged by size:
2060 812 451 114 80 23
AvrII C'CTAG_G
Cuts at: 0 2211 2439 3540
Size: 2211 228 1101
Fragments arranged by size:
2211 1101 228
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 623 656 1808 1841 1875 1908 3540
Size: 623 33 1152 33 34 33 1632
Fragments arranged by size:
1632 1152 623 34 33 33 33
BamHI G'GATC_C
Cuts at: 0 2012 3540
Size: 2012 1528
BanI G'GyrC_C
Cuts at: 0 872 2854 2937 3172 3540
Size: 872 1982 83 235 368
Fragments arranged by size:
1982 872 368 235 83
BanII G_rGCy'C
Cuts at: 0 117 1232 2611 2658 2873 2995 3070 3128
Size: 117 1115 1379 47 215 122 75 58
Cuts at: 3128 3334 3529 3540
Size: 206 195 11
Fragments arranged by size:
1379 1115 215 206 195 122 117 75
58 47 11
BbeI G_GCGC'C
Cuts at: 0 876 2858 2941 3176 3540
Size: 876 1982 83 235 364
Fragments arranged by size:
1982 876 364 235 83
BbsI GAAGACnn'nnnn_
Cuts at: 0 1791 3540
Size: 1791 1749
BbvCI CC'TCA_GC
Cuts at: 0 2921 3540
Size: 2921 619
BciVI GTATCCnnnnn_n'
Cuts at: 0 2227 3484 3540
Size: 2227 1257 56
Fragments arranged by size:
2227 1257 56
BclI T'GATC_A
Cuts at: 0 1199 3540
Size: 1199 2341
BfrBI ATG'CAT
Cuts at: 0 2105 2160 2535 3540
Size: 2105 55 375 1005
Fragments arranged by size:
2105 1005 375 55
BglI GCCn_nnn'nGGC
Cuts at: 0 2434 2946 3540
Size: 2434 512 594
Fragments arranged by size:
2434 594 512
BglII A'GATC_T
Cuts at: 0 1522 2036 3540
Size: 1522 514 1504
Fragments arranged by size:
1522 1504 514
BlpI GC'TnA_GC
Cuts at: 0 587 2977 3540
Size: 587 2390 563
Fragments arranged by size:
2390 587 563
Bme1580I G_kGCm'C
Cuts at: 0 60 653 2076 2658 2873 3540
Size: 60 593 1423 582 215 667
Fragments arranged by size:
1423 667 593 582 215 60
BmrI ACTGGGnnnn_n'
Cuts at: 0 280 1349 1669 1699 3540
Size: 280 1069 320 30 1841
Fragments arranged by size:
1841 1069 320 280 30
BmtI G_CTAG'C
Cuts at: 0 2359 3540
Size: 2359 1181
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 364 3147 3540
Size: 364 2783 393
Fragments arranged by size:
2783 393 364
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 435 469 1370 3394 3540
Size: 435 34 901 2024 146
Fragments arranged by size:
2024 901 435 146 34
Bpu10I CC'TnA_GC
Cuts at: 0 2921 3540
Size: 2921 619
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1821 3540
Size: 1821 1719
BsaI GGTCTCn'nnnn_
Cuts at: 0 1960 3540
Size: 1960 1580
BsaAI yAC'GTr
Cuts at: 0 2651 3540
Size: 2651 889
BsaBI GATnn'nnATC
Cuts at: 0 721 947 1019 3540
Size: 721 226 72 2521
Fragments arranged by size:
2521 721 226 72
BsaHI Gr'CG_yC
Cuts at: 0 873 2855 2885 2938 3167 3173 3540
Size: 873 1982 30 53 229 6 367
Fragments arranged by size:
1982 873 367 229 53 30 6
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1606 1636 3540
Size: 1606 30 1904
Fragments arranged by size:
1904 1606 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 87 387 390 2601 2938 3048 3391 3519
Size: 87 300 3 2211 337 110 343 128
Cuts at: 3519 3540
Size: 21
Fragments arranged by size:
2211 343 337 300 128 110 87 21
3
BseYI C'CCAG_C
Cuts at: 0 917 1962 2075 2815 3157 3310 3540
Size: 917 1045 113 740 342 153 230
Fragments arranged by size:
1045 917 740 342 230 153 113
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1551 2244 3013 3540
Size: 1551 693 769 527
Fragments arranged by size:
1551 769 693 527
BsiEI CG_ry'CG
Cuts at: 0 2744 2950 3341 3359 3540
Size: 2744 206 391 18 181
Fragments arranged by size:
2744 391 206 181 18
BsiHKAI G_wGCw'C
Cuts at: 0 60 117 653 2770 2930 3128 3436 3529
Size: 60 57 536 2117 160 198 308 93
Cuts at: 3529 3540
Size: 11
Fragments arranged by size:
2117 536 308 198 160 93 60 57
11
BsmI GAATG_Cn'
Cuts at: 0 194 300 2174 3540
Size: 194 106 1874 1366
Fragments arranged by size:
1874 1366 194 106
BsmBI CGTCTCn'nnnn_
Cuts at: 0 3488 3540
Size: 3488 52
Bsp1286I G_dGCh'C
Cuts at: 0 60 117 653 1232 2076 2611 2658 2770
Size: 60 57 536 579 844 535 47 112
Cuts at: 2770 2873 2930 2995 3070 3128 3334 3436 3529
Size: 103 57 65 75 58 206 102 93
Cuts at: 3529 3540
Size: 11
Fragments arranged by size:
844 579 536 535 206 112 103 102
93 75 65 60 58 57 57 47
11
BspHI T'CATG_A
Cuts at: 0 156 563 2060 3540
Size: 156 407 1497 1480
Fragments arranged by size:
1497 1480 407 156
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 2350 3540
Size: 2350 1190
BsrBI CCG'CTC
Cuts at: 0 413 3180 3540
Size: 413 2767 360
Fragments arranged by size:
2767 413 360
BsrDI GCAATG_nn'
Cuts at: 0 1739 1904 3540
Size: 1739 165 1636
Fragments arranged by size:
1739 1636 165
BsrFI r'CCGG_y
Cuts at: 0 2857 2887 3258 3340 3540
Size: 2857 30 371 82 200
Fragments arranged by size:
2857 371 200 82 30
BsrGI T'GTAC_A
Cuts at: 0 1600 2333 2465 3540
Size: 1600 733 132 1075
Fragments arranged by size:
1600 1075 733 132
BssHII G'CGCG_C
Cuts at: 0 3046 3281 3540
Size: 3046 235 259
Fragments arranged by size:
3046 259 235
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1652 3540
Size: 1652 1888
BstEII G'GTnAC_C
Cuts at: 0 1726 3096 3540
Size: 1726 1370 444
Fragments arranged by size:
1726 1370 444
BstYI r'GATC_y
Cuts at: 0 531 1522 2012 2036 2572 3209 3540
Size: 531 991 490 24 536 637 331
Fragments arranged by size:
991 637 536 531 490 331 24
BtgI C'CryG_G
Cuts at: 0 48 602 2522 2778 2831 3149 3290 3335
Size: 48 554 1920 256 53 318 141 45
Cuts at: 3335 3540
Size: 205
Fragments arranged by size:
1920 554 318 256 205 141 53 48
45
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 708 3540
Size: 708 2832
BtsI GCAGTG_nn'
Cuts at: 0 915 1539 3096 3540
Size: 915 624 1557 444
Fragments arranged by size:
1557 915 624 444
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1767 1802 3540
Size: 1767 35 1738
Fragments arranged by size:
1767 1738 35
DraIII CAC_nnn'GTG
Cuts at: 0 2405 3540
Size: 2405 1135
EaeI y'GGCC_r
Cuts at: 0 863 1033 1775 2477 2741 2912 2947 2996
Size: 863 170 742 702 264 171 35 49
Cuts at: 2996 3110 3260 3338 3356 3540
Size: 114 150 78 18 184
Fragments arranged by size:
863 742 702 264 184 171 170 150
114 78 49 35 18
EagI C'GGCC_G
Cuts at: 0 2741 2947 3338 3356 3540
Size: 2741 206 391 18 184
Fragments arranged by size:
2741 391 206 184 18
EarI CTCTTCn'nnn_
Cuts at: 0 356 408 613 2593 2662 3540
Size: 356 52 205 1980 69 878
Fragments arranged by size:
1980 878 356 205 69 52
EcoICRI GAG'CTC
Cuts at: 0 115 3126 3527 3540
Size: 115 3011 401 13
Fragments arranged by size:
3011 401 115 13
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 341 435 469 563 802 1045 1370 1921
Size: 341 94 34 94 239 243 325 551
Cuts at: 1921 3393 3394 3540
Size: 1472 1 146
Fragments arranged by size:
1472 551 341 325 243 239 146 94
94 34 1
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1839 3540
Size: 1839 1701
EcoRV GAT'ATC
Cuts at: 0 949 3540
Size: 949 2591
FspI TGC'GCA
Cuts at: 0 2500 2530 2850 3540
Size: 2500 30 320 690
Fragments arranged by size:
2500 690 320 30
FspAI rTGC'GCAy
Cuts at: 0 2530 3540
Size: 2530 1010
HaeII r_GCGC'y
Cuts at: 0 876 2507 2700 2792 2798 2816 2858 2902
Size: 876 1631 193 92 6 18 42 44
Cuts at: 2902 2941 3176 3540
Size: 39 235 364
Fragments arranged by size:
1631 876 364 235 193 92 44 42
39 18 6
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 382 414 1184 1216 1343 1375 2536 2568
Size: 382 32 770 32 127 32 1161 32
Cuts at: 2568 3007 3039 3540
Size: 439 32 501
Fragments arranged by size:
1161 770 501 439 382 127 32 32
32 32 32
HincII GTy'rAC
Cuts at: 0 2589 3540
Size: 2589 951
KasI G'GCGC_C
Cuts at: 0 872 2854 2937 3172 3540
Size: 872 1982 83 235 368
Fragments arranged by size:
1982 872 368 235 83
MfeI C'AATT_G
Cuts at: 0 1292 3540
Size: 1292 2248
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1431 1573 3540
Size: 1431 142 1967
Fragments arranged by size:
1967 1431 142
MscI TGG'CCA
Cuts at: 0 865 1777 2479 3540
Size: 865 912 702 1061
Fragments arranged by size:
1061 912 865 702
MslI CAynn'nnrTG
Cuts at: 0 844 1243 2401 2452 3226 3540
Size: 844 399 1158 51 774 314
Fragments arranged by size:
1158 844 774 399 314 51
MspA1I CmG'CkG
Cuts at: 0 1586 1747 3151 3292 3337 3540
Size: 1586 161 1404 141 45 203
Fragments arranged by size:
1586 1404 203 161 141 45
NaeI GCC'GGC
Cuts at: 0 2859 2889 3260 3540
Size: 2859 30 371 280
Fragments arranged by size:
2859 371 280 30
NarI GG'CG_CC
Cuts at: 0 873 2855 2938 3173 3540
Size: 873 1982 83 235 367
Fragments arranged by size:
1982 873 367 235 83
NcoI C'CATG_G
Cuts at: 0 602 3540
Size: 602 2938
NdeI CA'TA_TG
Cuts at: 0 2101 3540
Size: 2101 1439
NgoMIV G'CCGG_C
Cuts at: 0 2857 2887 3258 3540
Size: 2857 30 371 282
Fragments arranged by size:
2857 371 282 30
NheI G'CTAG_C
Cuts at: 0 2355 3540
Size: 2355 1185
NotI GC'GGCC_GC
Cuts at: 0 2947 3356 3540
Size: 2947 409 184
Fragments arranged by size:
2947 409 184
NsiI A_TGCA'T
Cuts at: 0 2107 2162 2537 3540
Size: 2107 55 375 1003
Fragments arranged by size:
2107 1003 375 55
NspI r_CATG'y
Cuts at: 0 93 1242 1646 1655 2451 2535 2539 3540
Size: 93 1149 404 9 796 84 4 1001
Fragments arranged by size:
1149 1001 796 404 93 84 9 4
PflMI CCAn_nnn'nTGG
Cuts at: 0 1297 3540
Size: 1297 2243
PmlI CAC'GTG
Cuts at: 0 2651 3540
Size: 2651 889
PsiI TTA'TAA
Cuts at: 0 2137 3540
Size: 2137 1403
PspOMI G'GGCC_C
Cuts at: 0 2654 2869 3540
Size: 2654 215 671
Fragments arranged by size:
2654 671 215
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 1111 1143 3540
Size: 1111 32 2397
Fragments arranged by size:
2397 1111 32
PvuII CAG'CTG
Cuts at: 0 1586 1747 3540
Size: 1586 161 1793
Fragments arranged by size:
1793 1586 161
RsrII CG'GwC_CG
Cuts at: 0 177 3540
Size: 177 3363
SacI G_AGCT'C
Cuts at: 0 117 3128 3529 3540
Size: 117 3011 401 11
Fragments arranged by size:
3011 401 117 11
SacII CC_GC'GG
Cuts at: 0 3152 3293 3338 3540
Size: 3152 141 45 202
Fragments arranged by size:
3152 202 141 45
SapI GCTCTTCn'nnn_
Cuts at: 0 2662 3540
Size: 2662 878
SfcI C'TryA_G
Cuts at: 0 458 744 1793 3540
Size: 458 286 1049 1747
Fragments arranged by size:
1747 1049 458 286
SfoI GGC'GCC
Cuts at: 0 874 2856 2939 3174 3540
Size: 874 1982 83 235 366
Fragments arranged by size:
1982 874 366 235 83
SmaI CCC'GGG
Cuts at: 0 814 3325 3540
Size: 814 2511 215
Fragments arranged by size:
2511 814 215
SmlI C'TyrA_G
Cuts at: 0 1836 2625 3540
Size: 1836 789 915
Fragments arranged by size:
1836 915 789
SphI G_CATG'C
Cuts at: 0 1655 2535 3540
Size: 1655 880 1005
Fragments arranged by size:
1655 1005 880
SspI AAT'ATT
Cuts at: 0 559 3540
Size: 559 2981
StyI C'CwwG_G
Cuts at: 0 602 806 1057 1764 2211 2439 2894 3540
Size: 602 204 251 707 447 228 455 646
Fragments arranged by size:
707 646 602 455 447 251 228 204
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 2403 3540
Size: 2403 1137
TatI w'GTAC_w
Cuts at: 0 1600 1637 1707 2333 2465 3540
Size: 1600 37 70 626 132 1075
Fragments arranged by size:
1600 1075 626 132 70 37
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 267 813 873 1215 1746 2520 3369 3540
Size: 267 546 60 342 531 774 849 171
Fragments arranged by size:
849 774 546 531 342 267 171 60
XmaI C'CCGG_G
Cuts at: 0 812 3323 3540
Size: 812 2511 217
Fragments arranged by size:
2511 812 217
XmnI GAAnn'nnTTC
Cuts at: 0 1106 3540
Size: 1106 2434
Enzymes that do cut:
AccI AclI AcuI AfeI AflII AlfI AlwNI ApaI
ApaLI ApoI AvaI AvrII BaeI BamHI BanI BanII
BbeI BbsI BbvCI BciVI BclI BfrBI BglI BglII
BlpI Bme1580I BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BstAPI BstEII BstYI BtgI
BtgZI BtsI CspCI DraIII EaeI EagI EarI EcoICRI
Eco57MI EcoNI EcoRV FspI FspAI HaeII Hin4I HincII
KasI MfeI MmeI MscI MslI MspA1I NaeI NarI
NcoI NdeI NgoMIV NheI NotI NsiI NspI PflMI
PmlI PsiI PspOMI PsrI PvuII RsrII SacI SacII
SapI SfcI SfoI SmaI SmlI SphI SspI StyI
TaqII TatI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AflIII AgeI AhdI AleI AloI
AscI AseI AsiSI BcgI BmgBI BsaWI BsiWI BspEI
BssSI BstBI BstXI BstZ17I Bsu36I ClaI DraI DrdI
EciI EcoO109I EcoRI FalI FseI HindIII HpaI KpnI
MluI NruI PacI PasI PciI PfoI PmeI PpiI
PpuMI PshAI PspXI PstI PvuI SalI SanDI SbfI
ScaI SexAI SfiI SgfI SgrAI SnaBI SpeI SrfI
StuI SwaI TstI Tth111I XbaI XhoI ZraI