Restriction map for
pFN21AE9554
(Linear) (Six-base) MAPSORT of: KIEE9554.seq Check: 8373 from: 1 to: 1047
KIEE9554.orf
With 182 enzymes: SgfI *
March 22, 2012 18:31 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 734 834 1047
Size: 734 100 213
Fragments arranged by size:
734 213 100
AflIII A'CryG_T
Cuts at: 0 698 1047
Size: 698 349
AleI CACnn'nnGTG
Cuts at: 0 109 301 983 1047
Size: 109 192 682 64
Fragments arranged by size:
682 192 109 64
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 472 1047
Size: 472 575
ApaLI G'TGCA_C
Cuts at: 0 412 606 1047
Size: 412 194 441
Fragments arranged by size:
441 412 194
ApoI r'AATT_y
Cuts at: 0 924 1047
Size: 924 123
AvaI C'yCGr_G
Cuts at: 0 67 1047
Size: 67 980
BanI G'GyrC_C
Cuts at: 0 310 1047
Size: 310 737
BanII G_rGCy'C
Cuts at: 0 53 68 1047
Size: 53 15 979
Fragments arranged by size:
979 53 15
BbeI G_GCGC'C
Cuts at: 0 314 1047
Size: 314 733
BbsI GAAGACnn'nnnn_
Cuts at: 0 609 1047
Size: 609 438
BbvCI CC'TCA_GC
Cuts at: 0 22 1047
Size: 22 1025
BclI T'GATC_A
Cuts at: 0 161 1047
Size: 161 886
BfrBI ATG'CAT
Cuts at: 0 910 1047
Size: 910 137
BglI GCCn_nnn'nGGC
Cuts at: 0 244 1047
Size: 244 803
Bme1580I G_kGCm'C
Cuts at: 0 416 610 1047
Size: 416 194 437
Fragments arranged by size:
437 416 194
Bpu10I CC'TnA_GC
Cuts at: 0 22 229 1047
Size: 22 207 818
Fragments arranged by size:
818 207 22
BsaAI yAC'GTr
Cuts at: 0 402 600 1047
Size: 402 198 447
Fragments arranged by size:
447 402 198
BsaHI Gr'CG_yC
Cuts at: 0 311 832 1047
Size: 311 521 215
Fragments arranged by size:
521 311 215
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 417 420 1047
Size: 417 3 627
Fragments arranged by size:
627 417 3
BseYI C'CCAG_C
Cuts at: 0 135 144 306 459 560 1047
Size: 135 9 162 153 101 487
Fragments arranged by size:
487 162 153 135 101 9
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 719 1047
Size: 719 328
BsiEI CG_ry'CG
Cuts at: 0 14 1047
Size: 14 1033
BsiHKAI G_wGCw'C
Cuts at: 0 205 416 610 1047
Size: 205 211 194 437
Fragments arranged by size:
437 211 205 194
Bsp1286I G_dGCh'C
Cuts at: 0 53 68 205 416 610 1047
Size: 53 15 137 211 194 437
Fragments arranged by size:
437 211 194 137 53 15
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 260 635 1047
Size: 260 375 412
Fragments arranged by size:
412 375 260
BsrBI CCG'CTC
Cuts at: 0 636 1047
Size: 636 411
BsrDI GCAATG_nn'
Cuts at: 0 488 1047
Size: 488 559
BsrFI r'CCGG_y
Cuts at: 0 834 1047
Size: 834 213
BssHII G'CGCG_C
Cuts at: 0 175 537 1047
Size: 175 362 510
Fragments arranged by size:
510 362 175
BstXI CCAn_nnnn'nTGG
Cuts at: 0 495 1047
Size: 495 552
Bsu36I CC'TnA_GG
Cuts at: 0 436 1047
Size: 436 611
BtgI C'CryG_G
Cuts at: 0 196 389 494 506 1047
Size: 196 193 105 12 541
Fragments arranged by size:
541 196 193 105 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 219 397 1047
Size: 219 178 650
Fragments arranged by size:
650 219 178
BtsI GCAGTG_nn'
Cuts at: 0 574 864 1047
Size: 574 290 183
Fragments arranged by size:
574 290 183
EaeI y'GGCC_r
Cuts at: 0 43 377 404 1047
Size: 43 334 27 643
Fragments arranged by size:
643 334 43 27
EarI CTCTTCn'nnn_
Cuts at: 0 262 532 853 1047
Size: 262 270 321 194
Fragments arranged by size:
321 270 262 194
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 734 834 1047
Size: 734 100 213
Fragments arranged by size:
734 213 100
EcoNI CCTnn'n_nnAGG
Cuts at: 0 25 435 1047
Size: 25 410 612
Fragments arranged by size:
612 410 25
EcoO109I rG'GnC_Cy
Cuts at: 0 522 666 1047
Size: 522 144 381
Fragments arranged by size:
522 381 144
HaeII r_GCGC'y
Cuts at: 0 314 426 484 1047
Size: 314 112 58 563
Fragments arranged by size:
563 314 112 58
KasI G'GCGC_C
Cuts at: 0 310 1047
Size: 310 737
MfeI C'AATT_G
Cuts at: 0 1032 1047
Size: 1032 15
MscI TGG'CCA
Cuts at: 0 406 1047
Size: 406 641
MslI CAynn'nnrTG
Cuts at: 0 88 109 301 358 493 983 1047
Size: 88 21 192 57 135 490 64
Fragments arranged by size:
490 192 135 88 64 57 21
MspA1I CmG'CkG
Cuts at: 0 75 198 828 1047
Size: 75 123 630 219
Fragments arranged by size:
630 219 123 75
NaeI GCC'GGC
Cuts at: 0 836 1047
Size: 836 211
NarI GG'CG_CC
Cuts at: 0 311 1047
Size: 311 736
NcoI C'CATG_G
Cuts at: 0 494 1047
Size: 494 553
NgoMIV G'CCGG_C
Cuts at: 0 834 1047
Size: 834 213
NsiI A_TGCA'T
Cuts at: 0 912 1047
Size: 912 135
NspI r_CATG'y
Cuts at: 0 702 1047
Size: 702 345
PciI A'CATG_T
Cuts at: 0 698 1047
Size: 698 349
PflMI CCAn_nnn'nTGG
Cuts at: 0 870 1047
Size: 870 177
PpuMI rG'GwC_Cy
Cuts at: 0 666 1047
Size: 666 381
SacII CC_GC'GG
Cuts at: 0 199 1047
Size: 199 848
SapI GCTCTTCn'nnn_
Cuts at: 0 262 1047
Size: 262 785
SfoI GGC'GCC
Cuts at: 0 312 1047
Size: 312 735
SmaI CCC'GGG
Cuts at: 0 69 1047
Size: 69 978
SrfI GCCC'GGGC
Cuts at: 0 69 1047
Size: 69 978
StuI AGG'CCT
Cuts at: 0 595 937 1047
Size: 595 342 110
Fragments arranged by size:
595 342 110
StyI C'CwwG_G
Cuts at: 0 494 662 1047
Size: 494 168 385
Fragments arranged by size:
494 385 168
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 291 1047
Size: 291 756
TatI w'GTAC_w
Cuts at: 0 1036 1047
Size: 1036 11
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 303 1047
Size: 303 744
XmaI C'CCGG_G
Cuts at: 0 67 1047
Size: 67 980
Enzymes that do cut:
AcuI AflIII AleI AlfI ApaLI ApoI AvaI BanI
BanII BbeI BbsI BbvCI BclI BfrBI BglI Bme1580I
Bpu10I BsaAI BsaHI BseRI BseYI BsgI BsiEI BsiHKAI
Bsp1286I BspMI BsrBI BsrDI BsrFI BssHII BstXI Bsu36I
BtgI BtgZI BtsI EaeI EarI Eco57MI EcoNI EcoO109I
HaeII KasI MfeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NsiI NspI PciI PflMI PpuMI SacII
SapI SfoI SmaI SrfI StuI StyI TaqII TatI
XcmI XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AgeI
AhdI AloI AlwNI ApaI AscI AseI AsiSI AvrII
BaeI BamHI BcgI BciVI BglII BlpI BmgBI BmrI
BmtI BplI BpmI BpuEI BsaI BsaBI BsaWI BsaXI
BsiWI BsmI BsmBI BspEI BspHI BsrGI BssSI BstAPI
BstBI BstEII BstYI BstZ17I ClaI CspCI DraI DraIII
DrdI EagI EciI EcoICRI EcoRI EcoRV FalI FseI
FspI FspAI Hin4I HincII HindIII HpaI KpnI MluI
MmeI NdeI NheI NotI NruI PacI PasI PfoI
PmeI PmlI PpiI PshAI PsiI PspOMI PspXI PsrI
PstI PvuI PvuII RsrII SacI SalI SanDI SbfI
ScaI SexAI SfcI SfiI SgfI SgrAI SmlI SnaBI
SpeI SphI SspI SwaI TstI Tth111I XbaI XhoI
XmnI ZraI