Restriction map for
pFN21AE9553
(Linear) (Six-base) MAPSORT of: KIEE9553.seq Check: 1840 from: 1 to: 2118
KIEE9553.orf
With 182 enzymes: SgfI *
December 27, 2011 16:42 ..
AccI GT'mk_AC
Cuts at: 0 1898 2118
Size: 1898 220
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1242 1672 2006 2118
Size: 1242 430 334 112
Fragments arranged by size:
1242 430 334 112
AflII C'TTAA_G
Cuts at: 0 493 2118
Size: 493 1625
AflIII A'CryG_T
Cuts at: 0 25 560 2118
Size: 25 535 1558
Fragments arranged by size:
1558 535 25
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2020 2118
Size: 2020 98
ApoI r'AATT_y
Cuts at: 0 263 424 1234 2118
Size: 263 161 810 884
Fragments arranged by size:
884 810 263 161
AseI AT'TA_AT
Cuts at: 0 325 1450 2118
Size: 325 1125 668
Fragments arranged by size:
1125 668 325
AvrII C'CTAG_G
Cuts at: 0 822 1740 2118
Size: 822 918 378
Fragments arranged by size:
918 822 378
BamHI G'GATC_C
Cuts at: 0 341 2118
Size: 341 1777
BanII G_rGCy'C
Cuts at: 0 1148 2118
Size: 1148 970
BbsI GAAGACnn'nnnn_
Cuts at: 0 1248 1464 2118
Size: 1248 216 654
Fragments arranged by size:
1248 654 216
BclI T'GATC_A
Cuts at: 0 1353 2118
Size: 1353 765
BglI GCCn_nnn'nGGC
Cuts at: 0 1916 2118
Size: 1916 202
Bme1580I G_kGCm'C
Cuts at: 0 56 2118
Size: 56 2062
BmgBI CAC'GTC
Cuts at: 0 26 2118
Size: 26 2092
BmrI ACTGGGnnnn_n'
Cuts at: 0 2009 2118
Size: 2009 109
BmtI G_CTAG'C
Cuts at: 0 1817 2118
Size: 1817 301
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1860 2118
Size: 1860 258
BsaI GGTCTCn'nnnn_
Cuts at: 0 687 1353 2118
Size: 687 666 765
Fragments arranged by size:
765 687 666
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 370 2118
Size: 370 1748
BseYI C'CCAG_C
Cuts at: 0 1510 2118
Size: 1510 608
Bsp1286I G_dGCh'C
Cuts at: 0 56 1148 2118
Size: 56 1092 970
Fragments arranged by size:
1092 970 56
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 259 1860 1998 2118
Size: 259 1601 138 120
Fragments arranged by size:
1601 259 138 120
BstAPI GCAn_nnn'nTGC
Cuts at: 0 776 2118
Size: 776 1342
BstBI TT'CG_AA
Cuts at: 0 1245 2118
Size: 1245 873
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1671 2118
Size: 1671 447
BstYI r'GATC_y
Cuts at: 0 341 1539 2118
Size: 341 1198 579
Fragments arranged by size:
1198 579 341
Bsu36I CC'TnA_GG
Cuts at: 0 353 2118
Size: 353 1765
BtgI C'CryG_G
Cuts at: 0 161 1670 2118
Size: 161 1509 448
Fragments arranged by size:
1509 448 161
BtsI GCAGTG_nn'
Cuts at: 0 977 1926 2118
Size: 977 949 192
Fragments arranged by size:
977 949 192
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1041 1076 2118
Size: 1041 35 1042
Fragments arranged by size:
1042 1041 35
DraI TTT'AAA
Cuts at: 0 570 1521 2118
Size: 570 951 597
Fragments arranged by size:
951 597 570
DraIII CAC_nnn'GTG
Cuts at: 0 893 2118
Size: 893 1225
DrdI GACnn_nn'nnGTC
Cuts at: 0 817 2118
Size: 817 1301
EaeI y'GGCC_r
Cuts at: 0 1209 2118
Size: 1209 909
EarI CTCTTCn'nnn_
Cuts at: 0 67 1157 1554 2118
Size: 67 1090 397 564
Fragments arranged by size:
1090 564 397 67
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1242 1672 1860 2006 2118
Size: 1242 430 188 146 112
Fragments arranged by size:
1242 430 188 146 112
EcoO109I rG'GnC_Cy
Cuts at: 0 2010 2118
Size: 2010 108
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1289 1321 1529 1561 2118
Size: 1289 32 208 32 557
Fragments arranged by size:
1289 557 208 32 32
HincII GTy'rAC
Cuts at: 0 23 1899 2118
Size: 23 1876 219
Fragments arranged by size:
1876 219 23
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1461 2118
Size: 1461 657
MspA1I CmG'CkG
Cuts at: 0 163 1262 2118
Size: 163 1099 856
Fragments arranged by size:
1099 856 163
NcoI C'CATG_G
Cuts at: 0 1670 2118
Size: 1670 448
NheI G'CTAG_C
Cuts at: 0 1813 2118
Size: 1813 305
NspI r_CATG'y
Cuts at: 0 564 1499 2118
Size: 564 935 619
Fragments arranged by size:
935 619 564
PciI A'CATG_T
Cuts at: 0 560 2118
Size: 560 1558
PflMI CCAn_nnn'nTGG
Cuts at: 0 917 2118
Size: 917 1201
PpuMI rG'GwC_Cy
Cuts at: 0 2010 2118
Size: 2010 108
PstI C_TGCA'G
Cuts at: 0 933 1716 2118
Size: 933 783 402
Fragments arranged by size:
933 783 402
SacII CC_GC'GG
Cuts at: 0 164 2118
Size: 164 1954
SalI G'TCGA_C
Cuts at: 0 1897 2118
Size: 1897 221
ScaI AGT'ACT
Cuts at: 0 1565 2118
Size: 1565 553
SexAI A'CCwGG_T
Cuts at: 0 1002 2118
Size: 1002 1116
SfcI C'TryA_G
Cuts at: 0 929 1567 1691 1712 2118
Size: 929 638 124 21 406
Fragments arranged by size:
929 638 406 124 21
SmlI C'TyrA_G
Cuts at: 0 493 2118
Size: 493 1625
SspI AAT'ATT
Cuts at: 0 620 2118
Size: 620 1498
StyI C'CwwG_G
Cuts at: 0 484 822 1670 1740 2118
Size: 484 338 848 70 378
Fragments arranged by size:
848 484 378 338 70
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 888 2118
Size: 888 1230
TatI w'GTAC_w
Cuts at: 0 740 1110 1563 2118
Size: 740 370 453 555
Fragments arranged by size:
740 555 453 370
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 992 1024 2118
Size: 992 32 1094
Fragments arranged by size:
1094 992 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 466 2118
Size: 466 1652
XmnI GAAnn'nnTTC
Cuts at: 0 263 612 2118
Size: 263 349 1506
Fragments arranged by size:
1506 349 263
Enzymes that do cut:
AccI AcuI AflII AflIII AlfI ApoI AseI AvrII
BamHI BanII BbsI BclI BglI Bme1580I BmgBI BmrI
BmtI BpmI BsaI BseRI BseYI Bsp1286I BspMI BstAPI
BstBI BstXI BstYI Bsu36I BtgI BtsI CspCI DraI
DraIII DrdI EaeI EarI Eco57MI EcoO109I Hin4I HincII
MmeI MspA1I NcoI NheI NspI PciI PflMI PpuMI
PstI SacII SalI ScaI SexAI SfcI SmlI SspI
StyI TaqII TatI TstI XcmI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AgeI AhdI AleI
AloI AlwNI ApaI ApaLI AscI AsiSI AvaI BaeI
BanI BbeI BbvCI BcgI BciVI BfrBI BglII BlpI
BplI Bpu10I BpuEI BsaAI BsaBI BsaHI BsaWI BsaXI
BsgI BsiEI BsiHKAI BsiWI BsmI BsmBI BspEI BspHI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstEII BstZ17I
BtgZI ClaI EagI EciI EcoICRI EcoNI EcoRI EcoRV
FalI FseI FspI FspAI HaeII HindIII HpaI KasI
KpnI MfeI MluI MscI MslI NaeI NarI NdeI
NgoMIV NotI NruI NsiI PacI PasI PfoI PmeI
PmlI PpiI PshAI PsiI PspOMI PspXI PsrI PvuI
PvuII RsrII SacI SanDI SapI SbfI SfiI SfoI
SgfI SgrAI SmaI SnaBI SpeI SphI SrfI StuI
SwaI Tth111I XbaI XhoI XmaI ZraI