(Linear) (Six-base) MAPSORT of: KIEE9551.seq  Check: 2505  from: 1  to: 1020



KIEE9551.orf

 With 182 enzymes: SgfI * 

                        December 27, 2011 16:42  ..

AccI GT'mk_AC

Cuts at:      0     509     589    1020 
   Size:        509      80     431

  Fragments arranged by size: 

                 509     431      80
 
AflIII A'CryG_T

Cuts at:      0     424    1020 
   Size:        424     596
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     117     149    1020 
   Size:        117      32     871

  Fragments arranged by size: 

                 871     117      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0     202     707    1020 
   Size:        202     505     313

  Fragments arranged by size: 

                 505     313     202
 
ApaI G_GGCC'C

Cuts at:      0     282    1020 
   Size:        282     738
 
ApoI r'AATT_y

Cuts at:      0     526     933    1020 
   Size:        526     407      87

  Fragments arranged by size: 

                 526     407      87
 
AvaI C'yCGr_G

Cuts at:      0     281    1020 
   Size:        281     739
 
BanII G_rGCy'C

Cuts at:      0     282    1020 
   Size:        282     738
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     330    1020 
   Size:        330     690
 
BfrBI ATG'CAT

Cuts at:      0      16    1020 
   Size:         16    1004
 
Bme1580I G_kGCm'C

Cuts at:      0     282    1020 
   Size:        282     738
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     710    1020 
   Size:        710     310
 
BsaWI w'CCGG_w

Cuts at:      0     452    1020 
   Size:        452     568
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     281     311    1020 
   Size:        281      30     709

  Fragments arranged by size: 

                 709     281      30
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     300    1020 
   Size:        300     720
 
BsmI GAATG_Cn'

Cuts at:      0     260    1020 
   Size:        260     760
 
Bsp1286I G_dGCh'C

Cuts at:      0     282    1020 
   Size:        282     738
 
BspEI T'CCGG_A

Cuts at:      0     452    1020 
   Size:        452     568
 
BspHI T'CATG_A

Cuts at:      0     368    1020 
   Size:        368     652
 
BsrDI GCAATG_nn'

Cuts at:      0     493    1020 
   Size:        493     527
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     250    1020 
   Size:        250     770
 
BstYI r'GATC_y

Cuts at:      0     181    1020 
   Size:        181     839
 
BstZ17I GTA'TAC

Cuts at:      0     590    1020 
   Size:        590     430
 
DraI TTT'AAA

Cuts at:      0      47     531    1020 
   Size:         47     484     489

  Fragments arranged by size: 

                 489     484      47
 
EarI CTCTTCn'nnn_

Cuts at:      0     960    1020 
   Size:        960      60
 
EcoO109I rG'GnC_Cy

Cuts at:      0     307    1020 
   Size:        307     713
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     550     582    1020 
   Size:        550      32     438

  Fragments arranged by size: 

                 550     438      32
 
HincII GTy'rAC

Cuts at:      0     746    1020 
   Size:        746     274
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0       4     231     409     432    1020 
   Size:          4     227     178      23     588

  Fragments arranged by size: 

                 588     227     178      23       4
 
NsiI A_TGCA'T

Cuts at:      0      18    1020 
   Size:         18    1002
 
PfoI T'CCnGG_A

Cuts at:      0      55     184    1020 
   Size:         55     129     836

  Fragments arranged by size: 

                 836     129      55
 
PspOMI G'GGCC_C

Cuts at:      0     278    1020 
   Size:        278     742
 
SapI GCTCTTCn'nnn_

Cuts at:      0     960    1020 
   Size:        960      60
 
SfcI C'TryA_G

Cuts at:      0     357    1020 
   Size:        357     663
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     285    1020 
   Size:        285     735
 
TatI w'GTAC_w

Cuts at:      0     579    1020 
   Size:        579     441
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     281     313    1020 
   Size:        281      32     707

  Fragments arranged by size: 

                 707     281      32
 

 Enzymes that do cut:

     AccI   AflIII     AloI    AlwNI     ApaI     ApoI     AvaI    BanII
    BciVI    BfrBI Bme1580I     BmrI    BsaWI    BsaXI     BsgI     BsmI
 Bsp1286I    BspEI    BspHI    BsrDI    BstXI    BstYI  BstZ17I     DraI
     EarI EcoO109I    Hin4I   HincII     MmeI     NsiI     PfoI   PspOMI
     SapI     SfcI    TaqII     TatI     TstI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AcuI     AfeI    AflII     AgeI
     AhdI     AleI     AlfI    ApaLI     AscI     AseI    AsiSI    AvrII
     BaeI    BamHI     BanI     BbeI     BbsI    BbvCI     BcgI     BclI
     BglI    BglII     BlpI    BmgBI     BmtI     BplI     BpmI   Bpu10I
    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BseRI    BseYI    BsiEI
  BsiHKAI    BsiWI    BsmBI    BspMI    BsrBI    BsrFI    BsrGI   BssHII
    BssSI   BstAPI    BstBI   BstEII   Bsu36I     BtgI    BtgZI     BtsI
     ClaI    CspCI   DraIII     DrdI     EaeI     EagI     EciI  EcoICRI
  Eco57MI    EcoNI    EcoRI    EcoRV     FalI     FseI     FspI    FspAI
    HaeII  HindIII     HpaI     KasI     KpnI     MfeI     MluI     MscI
     MslI   MspA1I     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI
     NotI     NruI     NspI     PacI     PasI     PciI    PflMI     PmeI
     PmlI     PpiI    PpuMI    PshAI     PsiI    PspXI     PsrI     PstI
     PvuI    PvuII    RsrII     SacI    SacII     SalI    SanDI     SbfI
     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI
    SnaBI     SpeI     SphI     SrfI     SspI     StuI     StyI     SwaI
  Tth111I     XbaI     XcmI     XhoI     XmaI     XmnI     ZraI