Restriction map for
pFN21AE9548
(Linear) (Six-base) MAPSORT of: KIEE9548.seq Check: 8660 from: 1 to: 1194
KIEE9548.orf
With 182 enzymes: SgfI *
December 27, 2011 16:42 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 731 1194
Size: 731 463
AccI GT'mk_AC
Cuts at: 0 863 1194
Size: 863 331
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 44 1194
Size: 44 1150
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1054 1086 1194
Size: 1054 32 108
Fragments arranged by size:
1054 108 32
AvaI C'yCGr_G
Cuts at: 0 1006 1194
Size: 1006 188
BanI G'GyrC_C
Cuts at: 0 64 529 706 1194
Size: 64 465 177 488
Fragments arranged by size:
488 465 177 64
BanII G_rGCy'C
Cuts at: 0 895 1194
Size: 895 299
BbsI GAAGACnn'nnnn_
Cuts at: 0 566 754 1194
Size: 566 188 440
Fragments arranged by size:
566 440 188
BglI GCCn_nnn'nGGC
Cuts at: 0 90 121 1194
Size: 90 31 1073
Fragments arranged by size:
1073 90 31
Bme1580I G_kGCm'C
Cuts at: 0 67 689 1194
Size: 67 622 505
Fragments arranged by size:
622 505 67
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 693 1194
Size: 693 501
BsaHI Gr'CG_yC
Cuts at: 0 1122 1194
Size: 1122 72
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1054 1084 1194
Size: 1054 30 110
Fragments arranged by size:
1054 110 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 571 1194
Size: 571 623
BseYI C'CCAG_C
Cuts at: 0 85 436 1078 1194
Size: 85 351 642 116
Fragments arranged by size:
642 351 116 85
BsiHKAI G_wGCw'C
Cuts at: 0 895 1194
Size: 895 299
Bsp1286I G_dGCh'C
Cuts at: 0 67 689 895 1194
Size: 67 622 206 299
Fragments arranged by size:
622 299 206 67
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 722 731 1194
Size: 722 9 463
Fragments arranged by size:
722 463 9
BsrBI CCG'CTC
Cuts at: 0 627 1194
Size: 627 567
BsrDI GCAATG_nn'
Cuts at: 0 377 1194
Size: 377 817
BsrGI T'GTAC_A
Cuts at: 0 902 1194
Size: 902 292
BssHII G'CGCG_C
Cuts at: 0 678 1194
Size: 678 516
BssSI C'ACGA_G
Cuts at: 0 595 1035 1194
Size: 595 440 159
Fragments arranged by size:
595 440 159
BstXI CCAn_nnnn'nTGG
Cuts at: 0 543 759 1194
Size: 543 216 435
Fragments arranged by size:
543 435 216
BtgI C'CryG_G
Cuts at: 0 59 518 752 976 1194
Size: 59 459 234 224 218
Fragments arranged by size:
459 234 224 218 59
BtsI GCAGTG_nn'
Cuts at: 0 689 1194
Size: 689 505
DraIII CAC_nnn'GTG
Cuts at: 0 539 597 1194
Size: 539 58 597
Fragments arranged by size:
597 539 58
EaeI y'GGCC_r
Cuts at: 0 137 303 398 884 1194
Size: 137 166 95 486 310
Fragments arranged by size:
486 310 166 137 95
EarI CTCTTCn'nnn_
Cuts at: 0 337 880 1194
Size: 337 543 314
Fragments arranged by size:
543 337 314
EcoICRI GAG'CTC
Cuts at: 0 893 1194
Size: 893 301
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 44 693 1194
Size: 44 649 501
Fragments arranged by size:
649 501 44
EcoO109I rG'GnC_Cy
Cuts at: 0 5 1194
Size: 5 1189
FspI TGC'GCA
Cuts at: 0 193 1194
Size: 193 1001
FspAI rTGC'GCAy
Cuts at: 0 193 1194
Size: 193 1001
HaeII r_GCGC'y
Cuts at: 0 229 1194
Size: 229 965
HincII GTy'rAC
Cuts at: 0 1044 1194
Size: 1044 150
MscI TGG'CCA
Cuts at: 0 400 886 1194
Size: 400 486 308
Fragments arranged by size:
486 400 308
MslI CAynn'nnrTG
Cuts at: 0 346 1194
Size: 346 848
MspA1I CmG'CkG
Cuts at: 0 89 566 968 1194
Size: 89 477 402 226
Fragments arranged by size:
477 402 226 89
NcoI C'CATG_G
Cuts at: 0 518 752 976 1194
Size: 518 234 224 218
Fragments arranged by size:
518 234 224 218
PflMI CCAn_nnn'nTGG
Cuts at: 0 597 785 1194
Size: 597 188 409
Fragments arranged by size:
597 409 188
PfoI T'CCnGG_A
Cuts at: 0 824 1194
Size: 824 370
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1054 1086 1194
Size: 1054 32 108
Fragments arranged by size:
1054 108 32
PpuMI rG'GwC_Cy
Cuts at: 0 5 1194
Size: 5 1189
PspXI vC'TCGA_Gb
Cuts at: 0 1006 1194
Size: 1006 188
PstI C_TGCA'G
Cuts at: 0 728 1194
Size: 728 466
PvuII CAG'CTG
Cuts at: 0 89 1194
Size: 89 1105
SacI G_AGCT'C
Cuts at: 0 895 1194
Size: 895 299
SbfI CC_TGCA'GG
Cuts at: 0 728 1194
Size: 728 466
SfcI C'TryA_G
Cuts at: 0 648 724 1194
Size: 648 76 470
Fragments arranged by size:
648 470 76
SmlI C'TyrA_G
Cuts at: 0 1006 1194
Size: 1006 188
StyI C'CwwG_G
Cuts at: 0 443 518 752 976 992 1194
Size: 443 75 234 224 16 202
Fragments arranged by size:
443 234 224 202 75 16
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1002 1194
Size: 1002 192
TatI w'GTAC_w
Cuts at: 0 902 1194
Size: 902 292
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 93 117 516 540 543 759 1194
Size: 93 24 399 24 3 216 435
Fragments arranged by size:
435 399 216 93 24 24 3
XhoI C'TCGA_G
Cuts at: 0 1006 1194
Size: 1006 188
XmnI GAAnn'nnTTC
Cuts at: 0 331 1194
Size: 331 863
Enzymes that do cut:
AarI AccI AcuI AloI AvaI BanI BanII BbsI
BglI Bme1580I BpmI BsaHI BsaXI BseRI BseYI BsiHKAI
Bsp1286I BspMI BsrBI BsrDI BsrGI BssHII BssSI BstXI
BtgI BtsI DraIII EaeI EarI EcoICRI Eco57MI EcoO109I
FspI FspAI HaeII HincII MscI MslI MspA1I NcoI
PflMI PfoI PpiI PpuMI PspXI PstI PvuII SacI
SbfI SfcI SmlI StyI TaqII TatI XcmI XhoI
XmnI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII AflIII AgeI AhdI
AleI AlfI AlwNI ApaI ApaLI ApoI AscI AseI
AsiSI AvrII BaeI BamHI BbeI BbvCI BcgI BciVI
BclI BfrBI BglII BlpI BmgBI BmrI BmtI BplI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaWI BsgI BsiEI
BsiWI BsmI BsmBI BspEI BspHI BsrFI BstAPI BstBI
BstEII BstYI BstZ17I Bsu36I BtgZI ClaI CspCI DraI
DrdI EagI EciI EcoNI EcoRI EcoRV FalI FseI
Hin4I HindIII HpaI KasI KpnI MfeI MluI MmeI
NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI
NspI PacI PasI PciI PmeI PmlI PshAI PsiI
PspOMI PsrI PvuI RsrII SacII SalI SanDI SapI
ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI
SpeI SphI SrfI SspI StuI SwaI TstI Tth111I
XbaI XmaI ZraI