Restriction map for
pFN21AE9547
(Linear) (Six-base) MAPSORT of: KIEE9547.seq Check: 2375 from: 1 to: 1659
KIEE9547.orf
With 182 enzymes: SgfI *
December 27, 2011 16:42 ..
AatII G_ACGT'C
Cuts at: 0 698 1659
Size: 698 961
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1110 1659
Size: 1110 549
AflIII A'CryG_T
Cuts at: 0 308 1659
Size: 308 1351
AlwNI CAG_nnn'CTG
Cuts at: 0 28 407 1391 1659
Size: 28 379 984 268
Fragments arranged by size:
984 379 268 28
ApaI G_GGCC'C
Cuts at: 0 141 1659
Size: 141 1518
ApaLI G'TGCA_C
Cuts at: 0 965 1659
Size: 965 694
ApoI r'AATT_y
Cuts at: 0 1079 1659
Size: 1079 580
AvaI C'yCGr_G
Cuts at: 0 1574 1659
Size: 1574 85
AvrII C'CTAG_G
Cuts at: 0 1419 1659
Size: 1419 240
BamHI G'GATC_C
Cuts at: 0 196 1659
Size: 196 1463
BanI G'GyrC_C
Cuts at: 0 814 1540 1659
Size: 814 726 119
Fragments arranged by size:
814 726 119
BanII G_rGCy'C
Cuts at: 0 141 231 388 872 1276 1659
Size: 141 90 157 484 404 383
Fragments arranged by size:
484 404 383 157 141 90
BbeI G_GCGC'C
Cuts at: 0 1544 1659
Size: 1544 115
BbsI GAAGACnn'nnnn_
Cuts at: 0 693 1659
Size: 693 966
BciVI GTATCCnnnnn_n'
Cuts at: 0 547 1659
Size: 547 1112
BclI T'GATC_A
Cuts at: 0 300 1218 1659
Size: 300 918 441
Fragments arranged by size:
918 441 300
BglI GCCn_nnn'nGGC
Cuts at: 0 1379 1659
Size: 1379 280
BlpI GC'TnA_GC
Cuts at: 0 1334 1659
Size: 1334 325
Bme1580I G_kGCm'C
Cuts at: 0 141 969 1659
Size: 141 828 690
Fragments arranged by size:
828 690 141
BmrI ACTGGGnnnn_n'
Cuts at: 0 1359 1659
Size: 1359 300
BmtI G_CTAG'C
Cuts at: 0 1369 1659
Size: 1369 290
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 277 1659
Size: 277 1382
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 886 994 1659
Size: 886 108 665
Fragments arranged by size:
886 665 108
Bpu10I CC'TnA_GC
Cuts at: 0 387 416 1659
Size: 387 29 1243
Fragments arranged by size:
1243 387 29
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 226 247 1069 1659
Size: 226 21 822 590
Fragments arranged by size:
822 590 226 21
BsaAI yAC'GTr
Cuts at: 0 309 1659
Size: 309 1350
BsaHI Gr'CG_yC
Cuts at: 0 59 695 1541 1659
Size: 59 636 846 118
Fragments arranged by size:
846 636 118 59
BsaWI w'CCGG_w
Cuts at: 0 1412 1659
Size: 1412 247
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 700 730 1659
Size: 700 30 929
Fragments arranged by size:
929 700 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 586 1648 1659
Size: 586 1062 11
Fragments arranged by size:
1062 586 11
BseYI C'CCAG_C
Cuts at: 0 6 1536 1544 1659
Size: 6 1530 8 115
Fragments arranged by size:
1530 115 8 6
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 606 1617 1659
Size: 606 1011 42
Fragments arranged by size:
1011 606 42
BsiHKAI G_wGCw'C
Cuts at: 0 130 388 969 1276 1659
Size: 130 258 581 307 383
Fragments arranged by size:
581 383 307 258 130
BsmI GAATG_Cn'
Cuts at: 0 478 1659
Size: 478 1181
Bsp1286I G_dGCh'C
Cuts at: 0 130 141 231 388 872 969 1276 1659
Size: 130 11 90 157 484 97 307 383
Fragments arranged by size:
484 383 307 157 130 97 90 11
BspHI T'CATG_A
Cuts at: 0 1188 1659
Size: 1188 471
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 917 1659
Size: 917 742
BsrBI CCG'CTC
Cuts at: 0 782 1659
Size: 782 877
BsrGI T'GTAC_A
Cuts at: 0 542 1659
Size: 542 1117
BssSI C'ACGA_G
Cuts at: 0 64 1659
Size: 64 1595
BstBI TT'CG_AA
Cuts at: 0 609 1659
Size: 609 1050
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1629 1659
Size: 1629 30
BstYI r'GATC_y
Cuts at: 0 196 1193 1659
Size: 196 997 466
Fragments arranged by size:
997 466 196
BtgI C'CryG_G
Cuts at: 0 74 446 548 1616 1628 1659
Size: 74 372 102 1068 12 31
Fragments arranged by size:
1068 372 102 74 31 12
BtsI GCAGTG_nn'
Cuts at: 0 405 618 1605 1659
Size: 405 213 987 54
Fragments arranged by size:
987 405 213 54
DraIII CAC_nnn'GTG
Cuts at: 0 986 1659
Size: 986 673
EaeI y'GGCC_r
Cuts at: 0 1619 1659
Size: 1619 40
EarI CTCTTCn'nnn_
Cuts at: 0 374 583 1204 1640 1659
Size: 374 209 621 436 19
Fragments arranged by size:
621 436 374 209 19
EcoICRI GAG'CTC
Cuts at: 0 386 1274 1659
Size: 386 888 385
Fragments arranged by size:
888 386 385
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 886 994 1110 1659
Size: 886 108 116 549
Fragments arranged by size:
886 549 116 108
EcoNI CCTnn'n_nnAGG
Cuts at: 0 32 117 205 1659
Size: 32 85 88 1454
Fragments arranged by size:
1454 88 85 32
EcoO109I rG'GnC_Cy
Cuts at: 0 25 138 149 395 1033 1659
Size: 25 113 11 246 638 626
Fragments arranged by size:
638 626 246 113 25 11
HaeII r_GCGC'y
Cuts at: 0 1460 1544 1659
Size: 1460 84 115
Fragments arranged by size:
1460 115 84
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 90 122 285 317 1379 1411 1659
Size: 90 32 163 32 1062 32 248
Fragments arranged by size:
1062 248 163 90 32 32 32
HincII GTy'rAC
Cuts at: 0 527 1265 1659
Size: 527 738 394
Fragments arranged by size:
738 527 394
HindIII A'AGCT_T
Cuts at: 0 1087 1465 1659
Size: 1087 378 194
Fragments arranged by size:
1087 378 194
KasI G'GCGC_C
Cuts at: 0 1540 1659
Size: 1540 119
MscI TGG'CCA
Cuts at: 0 1621 1659
Size: 1621 38
MslI CAynn'nnrTG
Cuts at: 0 640 1659
Size: 640 1019
MspA1I CmG'CkG
Cuts at: 0 537 1659
Size: 537 1122
NarI GG'CG_CC
Cuts at: 0 1541 1659
Size: 1541 118
NcoI C'CATG_G
Cuts at: 0 74 1616 1628 1659
Size: 74 1542 12 31
Fragments arranged by size:
1542 74 31 12
NheI G'CTAG_C
Cuts at: 0 1365 1659
Size: 1365 294
NspI r_CATG'y
Cuts at: 0 248 1659
Size: 248 1411
PasI CC'CwG_GG
Cuts at: 0 92 142 950 1659
Size: 92 50 808 709
Fragments arranged by size:
808 709 92 50
PfoI T'CCnGG_A
Cuts at: 0 330 1659
Size: 330 1329
PpuMI rG'GwC_Cy
Cuts at: 0 1033 1659
Size: 1033 626
PsiI TTA'TAA
Cuts at: 0 1136 1659
Size: 1136 523
PspOMI G'GGCC_C
Cuts at: 0 137 1659
Size: 137 1522
PstI C_TGCA'G
Cuts at: 0 1387 1481 1659
Size: 1387 94 178
Fragments arranged by size:
1387 178 94
PvuII CAG'CTG
Cuts at: 0 537 1659
Size: 537 1122
SacI G_AGCT'C
Cuts at: 0 388 1276 1659
Size: 388 888 383
Fragments arranged by size:
888 388 383
SfcI C'TryA_G
Cuts at: 0 399 1383 1477 1659
Size: 399 984 94 182
Fragments arranged by size:
984 399 182 94
SfoI GGC'GCC
Cuts at: 0 1542 1659
Size: 1542 117
SmaI CCC'GGG
Cuts at: 0 1576 1659
Size: 1576 83
SmlI C'TyrA_G
Cuts at: 0 205 262 1084 1659
Size: 205 57 822 575
Fragments arranged by size:
822 575 205 57
StuI AGG'CCT
Cuts at: 0 726 845 1659
Size: 726 119 814
Fragments arranged by size:
814 726 119
StyI C'CwwG_G
Cuts at: 0 74 367 1419 1616 1628 1659
Size: 74 293 1052 197 12 31
Fragments arranged by size:
1052 293 197 74 31 12
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 731 802 1659
Size: 731 71 857
Fragments arranged by size:
857 731 71
TatI w'GTAC_w
Cuts at: 0 233 542 681 1659
Size: 233 309 139 978
Fragments arranged by size:
978 309 233 139
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 864 896 1659
Size: 864 32 763
Fragments arranged by size:
864 763 32
Tth111I GACn'n_nGTC
Cuts at: 0 697 1659
Size: 697 962
XmaI C'CCGG_G
Cuts at: 0 1574 1659
Size: 1574 85
XmnI GAAnn'nnTTC
Cuts at: 0 1408 1659
Size: 1408 251
ZraI GAC'GTC
Cuts at: 0 696 1659
Size: 696 963
Enzymes that do cut:
AatII AcuI AflIII AlwNI ApaI ApaLI ApoI AvaI
AvrII BamHI BanI BanII BbeI BbsI BciVI BclI
BglI BlpI Bme1580I BmrI BmtI BplI BpmI Bpu10I
BpuEI BsaAI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiHKAI BsmI Bsp1286I BspHI BspMI BsrBI BsrGI BssSI
BstBI BstXI BstYI BtgI BtsI DraIII EaeI EarI
EcoICRI Eco57MI EcoNI EcoO109I HaeII Hin4I HincII HindIII
KasI MscI MslI MspA1I NarI NcoI NheI NspI
PasI PfoI PpuMI PsiI PspOMI PstI PvuII SacI
SfcI SfoI SmaI SmlI StuI StyI TaqII TatI
TstI Tth111I XmaI XmnI ZraI
Enzymes that do not cut:
AarI AccI Acc65I AclI AfeI AflII AgeI AhdI
AleI AlfI AloI AscI AseI AsiSI BaeI BbvCI
BcgI BfrBI BglII BmgBI BsaI BsaBI BsiEI BsiWI
BsmBI BspEI BsrDI BsrFI BssHII BstAPI BstEII BstZ17I
Bsu36I BtgZI ClaI CspCI DraI DrdI EagI EciI
EcoRI EcoRV FalI FseI FspI FspAI HpaI KpnI
MfeI MluI MmeI NaeI NdeI NgoMIV NotI NruI
NsiI PacI PciI PflMI PmeI PmlI PpiI PshAI
PspXI PsrI PvuI RsrII SacII SalI SanDI SapI
SbfI ScaI SexAI SfiI SgfI SgrAI SnaBI SpeI
SphI SrfI SspI SwaI XbaI XcmI XhoI