Restriction map for
pFN21AE9535
(Linear) (Six-base) MAPSORT of: KIEE9535.seq Check: 49 from: 1 to: 2097
KIEE9535.orf
With 182 enzymes: SgfI *
December 27, 2011 16:41 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1747 2097
Size: 1747 350
AccI GT'mk_AC
Cuts at: 0 1361 2097
Size: 1361 736
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 52 2097
Size: 52 2045
AfeI AGC'GCT
Cuts at: 0 71 2097
Size: 71 2026
AleI CACnn'nnGTG
Cuts at: 0 836 2097
Size: 836 1261
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 124 1233 2097
Size: 124 1109 864
Fragments arranged by size:
1109 864 124
AlwNI CAG_nnn'CTG
Cuts at: 0 30 708 833 2097
Size: 30 678 125 1264
Fragments arranged by size:
1264 678 125 30
ApoI r'AATT_y
Cuts at: 0 447 461 870 1643 1918 2097
Size: 447 14 409 773 275 179
Fragments arranged by size:
773 447 409 275 179 14
AseI AT'TA_AT
Cuts at: 0 1106 1672 2097
Size: 1106 566 425
Fragments arranged by size:
1106 566 425
BanI G'GyrC_C
Cuts at: 0 118 1746 2097
Size: 118 1628 351
Fragments arranged by size:
1628 351 118
BanII G_rGCy'C
Cuts at: 0 98 347 1229 2097
Size: 98 249 882 868
Fragments arranged by size:
882 868 249 98
BbeI G_GCGC'C
Cuts at: 0 122 2097
Size: 122 1975
BbsI GAAGACnn'nnnn_
Cuts at: 0 423 2097
Size: 423 1674
BfrBI ATG'CAT
Cuts at: 0 577 2097
Size: 577 1520
BlpI GC'TnA_GC
Cuts at: 0 1912 2097
Size: 1912 185
Bme1580I G_kGCm'C
Cuts at: 0 113 2097
Size: 113 1984
BmgBI CAC'GTC
Cuts at: 0 752 2097
Size: 752 1345
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1489 2097
Size: 1489 608
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 625 649 826 2097
Size: 625 24 177 1271
Fragments arranged by size:
1271 625 177 24
Bpu10I CC'TnA_GC
Cuts at: 0 92 2097
Size: 92 2005
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 182 402 2097
Size: 182 220 1695
Fragments arranged by size:
1695 220 182
BsaI GGTCTCn'nnnn_
Cuts at: 0 154 2097
Size: 154 1943
BsaHI Gr'CG_yC
Cuts at: 0 119 2097
Size: 119 1978
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 799 829 2097
Size: 799 30 1268
Fragments arranged by size:
1268 799 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 280 426 2097
Size: 280 146 1671
Fragments arranged by size:
1671 280 146
BseYI C'CCAG_C
Cuts at: 0 826 983 2097
Size: 826 157 1114
Fragments arranged by size:
1114 826 157
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1335 2097
Size: 1335 762
BsiEI CG_ry'CG
Cuts at: 0 146 2097
Size: 146 1951
BsiHKAI G_wGCw'C
Cuts at: 0 1032 2097
Size: 1032 1065
BsmI GAATG_Cn'
Cuts at: 0 577 2097
Size: 577 1520
Bsp1286I G_dGCh'C
Cuts at: 0 98 113 347 1032 1229 2097
Size: 98 15 234 685 197 868
Fragments arranged by size:
868 685 234 197 98 15
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1747 2097
Size: 1747 350
BsrDI GCAATG_nn'
Cuts at: 0 2044 2097
Size: 2044 53
BsrFI r'CCGG_y
Cuts at: 0 145 2097
Size: 145 1952
BssSI C'ACGA_G
Cuts at: 0 65 2097
Size: 65 2032
BstAPI GCAn_nnn'nTGC
Cuts at: 0 708 2097
Size: 708 1389
BstBI TT'CG_AA
Cuts at: 0 1659 2097
Size: 1659 438
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1554 2097
Size: 1554 543
Bsu36I CC'TnA_GG
Cuts at: 0 1084 2097
Size: 1084 1013
BtgI C'CryG_G
Cuts at: 0 140 654 1547 2097
Size: 140 514 893 550
Fragments arranged by size:
893 550 514 140
BtsI GCAGTG_nn'
Cuts at: 0 831 1319 2097
Size: 831 488 778
Fragments arranged by size:
831 778 488
DraIII CAC_nnn'GTG
Cuts at: 0 2093 2097
Size: 2093 4
EaeI y'GGCC_r
Cuts at: 0 143 2097
Size: 143 1954
EagI C'GGCC_G
Cuts at: 0 143 2097
Size: 143 1954
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 52 625 649 826 2097
Size: 52 573 24 177 1271
Fragments arranged by size:
1271 573 177 52 24
EcoO109I rG'GnC_Cy
Cuts at: 0 705 1089 2097
Size: 705 384 1008
Fragments arranged by size:
1008 705 384
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1807 2097
Size: 1807 290
HaeII r_GCGC'y
Cuts at: 0 22 73 85 122 2097
Size: 22 51 12 37 1975
Fragments arranged by size:
1975 51 37 22 12
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 180 212 2097
Size: 180 32 1885
Fragments arranged by size:
1885 180 32
HincII GTy'rAC
Cuts at: 0 818 2097
Size: 818 1279
HindIII A'AGCT_T
Cuts at: 0 200 2097
Size: 200 1897
KasI G'GCGC_C
Cuts at: 0 118 2097
Size: 118 1979
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 2006 2097
Size: 2006 91
MslI CAynn'nnrTG
Cuts at: 0 836 1023 1552 1741 2097
Size: 836 187 529 189 356
Fragments arranged by size:
836 529 356 189 187
MspA1I CmG'CkG
Cuts at: 0 9 27 60 1774 2097
Size: 9 18 33 1714 323
Fragments arranged by size:
1714 323 33 18 9
NarI GG'CG_CC
Cuts at: 0 119 2097
Size: 119 1978
NcoI C'CATG_G
Cuts at: 0 1547 2097
Size: 1547 550
NsiI A_TGCA'T
Cuts at: 0 579 2097
Size: 579 1518
NspI r_CATG'y
Cuts at: 0 1447 2097
Size: 1447 650
PflMI CCAn_nnn'nTGG
Cuts at: 0 1295 2097
Size: 1295 802
PfoI T'CCnGG_A
Cuts at: 0 803 2097
Size: 803 1294
PpuMI rG'GwC_Cy
Cuts at: 0 1089 2097
Size: 1089 1008
PsiI TTA'TAA
Cuts at: 0 358 2097
Size: 358 1739
PstI C_TGCA'G
Cuts at: 0 26 53 2097
Size: 26 27 2044
Fragments arranged by size:
2044 27 26
PvuII CAG'CTG
Cuts at: 0 27 1774 2097
Size: 27 1747 323
Fragments arranged by size:
1747 323 27
ScaI AGT'ACT
Cuts at: 0 1276 2097
Size: 1276 821
SfcI C'TryA_G
Cuts at: 0 22 49 2097
Size: 22 27 2048
Fragments arranged by size:
2048 27 22
SfoI GGC'GCC
Cuts at: 0 120 2097
Size: 120 1977
SmlI C'TyrA_G
Cuts at: 0 197 381 2097
Size: 197 184 1716
Fragments arranged by size:
1716 197 184
SspI AAT'ATT
Cuts at: 0 1110 1874 2097
Size: 1110 764 223
Fragments arranged by size:
1110 764 223
StyI C'CwwG_G
Cuts at: 0 677 1265 1547 2097
Size: 677 588 282 550
Fragments arranged by size:
677 588 550 282
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1301 2097
Size: 1301 796
TatI w'GTAC_w
Cuts at: 0 564 1274 2097
Size: 564 710 823
Fragments arranged by size:
823 710 564
XbaI T'CTAG_A
Cuts at: 0 1383 2097
Size: 1383 714
XmnI GAAnn'nnTTC
Cuts at: 0 430 2097
Size: 430 1667
Enzymes that do cut:
AarI AccI AcuI AfeI AleI AlfI AlwNI ApoI
AseI BanI BanII BbeI BbsI BfrBI BlpI Bme1580I
BmgBI BplI BpmI Bpu10I BpuEI BsaI BsaHI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspMI
BsrDI BsrFI BssSI BstAPI BstBI BstXI Bsu36I BtgI
BtsI DraIII EaeI EagI Eco57MI EcoO109I FalI HaeII
Hin4I HincII HindIII KasI MmeI MslI MspA1I NarI
NcoI NsiI NspI PflMI PfoI PpuMI PsiI PstI
PvuII ScaI SfcI SfoI SmlI SspI StyI TaqII
TatI XbaI XmnI
Enzymes that do not cut:
AatII Acc65I AclI AflII AflIII AgeI AhdI AloI
ApaI ApaLI AscI AsiSI AvaI AvrII BaeI BamHI
BbvCI BcgI BciVI BclI BglI BglII BmrI BmtI
BsaAI BsaBI BsaWI BsiWI BsmBI BspEI BspHI BsrBI
BsrGI BssHII BstEII BstYI BstZ17I BtgZI ClaI CspCI
DraI DrdI EarI EciI EcoICRI EcoNI EcoRI EcoRV
FseI FspI FspAI HpaI KpnI MfeI MluI MscI
NaeI NdeI NgoMIV NheI NotI NruI PacI PasI
PciI PmeI PmlI PpiI PshAI PspOMI PspXI PsrI
PvuI RsrII SacI SacII SalI SanDI SapI SbfI
SexAI SfiI SgfI SgrAI SmaI SnaBI SpeI SphI
SrfI StuI SwaI TstI Tth111I XcmI XhoI XmaI
ZraI