Restriction map for
pFN21AE9533
(Linear) (Six-base) MAPSORT of: KIEE9533.seq Check: 1802 from: 1 to: 1230
KIEE9533.orf
With 182 enzymes: SgfI *
December 27, 2011 16:41 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 704 1230
Size: 704 526
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 193 1077 1181 1230
Size: 193 884 104 49
Fragments arranged by size:
884 193 104 49
AfeI AGC'GCT
Cuts at: 0 397 1230
Size: 397 833
AflIII A'CryG_T
Cuts at: 0 977 1230
Size: 977 253
AlwNI CAG_nnn'CTG
Cuts at: 0 420 1230
Size: 420 810
ApaLI G'TGCA_C
Cuts at: 0 160 340 579 1230
Size: 160 180 239 651
Fragments arranged by size:
651 239 180 160
AvaI C'yCGr_G
Cuts at: 0 42 538 735 1230
Size: 42 496 197 495
Fragments arranged by size:
496 495 197 42
BamHI G'GATC_C
Cuts at: 0 863 1209 1230
Size: 863 346 21
Fragments arranged by size:
863 346 21
BanI G'GyrC_C
Cuts at: 0 197 1230
Size: 197 1033
BanII G_rGCy'C
Cuts at: 0 272 566 1230
Size: 272 294 664
Fragments arranged by size:
664 294 272
BbeI G_GCGC'C
Cuts at: 0 201 1230
Size: 201 1029
BbsI GAAGACnn'nnnn_
Cuts at: 0 297 814 1104 1230
Size: 297 517 290 126
Fragments arranged by size:
517 297 290 126
BbvCI CC'TCA_GC
Cuts at: 0 787 1230
Size: 787 443
BciVI GTATCCnnnnn_n'
Cuts at: 0 1182 1230
Size: 1182 48
BglI GCCn_nnn'nGGC
Cuts at: 0 27 1230
Size: 27 1203
BlpI GC'TnA_GC
Cuts at: 0 49 217 370 1050 1230
Size: 49 168 153 680 180
Fragments arranged by size:
680 180 168 153 49
Bme1580I G_kGCm'C
Cuts at: 0 164 344 429 583 1230
Size: 164 180 85 154 647
Fragments arranged by size:
647 180 164 154 85
BmgBI CAC'GTC
Cuts at: 0 855 1230
Size: 855 375
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 585 1230
Size: 585 645
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 781 859 1230
Size: 781 78 371
Fragments arranged by size:
781 371 78
Bpu10I CC'TnA_GC
Cuts at: 0 385 787 1230
Size: 385 402 443
Fragments arranged by size:
443 402 385
BsaI GGTCTCn'nnnn_
Cuts at: 0 787 1230
Size: 787 443
BsaAI yAC'GTr
Cuts at: 0 165 213 966 1230
Size: 165 48 753 264
Fragments arranged by size:
753 264 165 48
BsaHI Gr'CG_yC
Cuts at: 0 59 198 442 1230
Size: 59 139 244 788
Fragments arranged by size:
788 244 139 59
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 815 845 1230
Size: 815 30 385
Fragments arranged by size:
815 385 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 775 790 1052 1077 1230
Size: 775 15 262 25 153
Fragments arranged by size:
775 262 153 25 15
BseYI C'CCAG_C
Cuts at: 0 31 66 132 751 1230
Size: 31 35 66 619 479
Fragments arranged by size:
619 479 66 35 31
BsiHKAI G_wGCw'C
Cuts at: 0 164 218 272 344 583 650 1139 1230
Size: 164 54 54 72 239 67 489 91
Fragments arranged by size:
489 239 164 91 72 67 54 54
BsmBI CGTCTCn'nnnn_
Cuts at: 0 535 1230
Size: 535 695
Bsp1286I G_dGCh'C
Cuts at: 0 164 218 272 344 429 566 583 650
Size: 164 54 54 72 85 137 17 67
Cuts at: 650 1139 1230
Size: 489 91
Fragments arranged by size:
489 164 137 91 85 72 67 54
54 17
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 704 1230
Size: 704 526
BsrBI CCG'CTC
Cuts at: 0 193 1230
Size: 193 1037
BsrDI GCAATG_nn'
Cuts at: 0 153 1230
Size: 153 1077
BsrFI r'CCGG_y
Cuts at: 0 261 1230
Size: 261 969
BsrGI T'GTAC_A
Cuts at: 0 995 1230
Size: 995 235
BssSI C'ACGA_G
Cuts at: 0 343 474 1230
Size: 343 131 756
Fragments arranged by size:
756 343 131
BstAPI GCAn_nnn'nTGC
Cuts at: 0 168 664 1230
Size: 168 496 566
Fragments arranged by size:
566 496 168
BstEII G'GTnAC_C
Cuts at: 0 1023 1154 1230
Size: 1023 131 76
Fragments arranged by size:
1023 131 76
BstXI CCAn_nnnn'nTGG
Cuts at: 0 507 759 1185 1230
Size: 507 252 426 45
Fragments arranged by size:
507 426 252 45
BstYI r'GATC_y
Cuts at: 0 863 1209 1230
Size: 863 346 21
Fragments arranged by size:
863 346 21
BtgI C'CryG_G
Cuts at: 0 16 25 324 362 506 1230
Size: 16 9 299 38 144 724
Fragments arranged by size:
724 299 144 38 16 9
DraIII CAC_nnn'GTG
Cuts at: 0 168 213 1230
Size: 168 45 1017
Fragments arranged by size:
1017 168 45
EaeI y'GGCC_r
Cuts at: 0 509 1230
Size: 509 721
EarI CTCTTCn'nnn_
Cuts at: 0 1070 1108 1230
Size: 1070 38 122
Fragments arranged by size:
1070 122 38
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 562 1230
Size: 562 668
EcoICRI GAG'CTC
Cuts at: 0 270 1230
Size: 270 960
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 193 781 859 1077 1181 1230
Size: 193 588 78 218 104 49
Fragments arranged by size:
588 218 193 104 78 49
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 828 1230
Size: 828 402
HaeII r_GCGC'y
Cuts at: 0 133 201 229 392 399 472 1230
Size: 133 68 28 163 7 73 758
Fragments arranged by size:
758 163 133 73 68 28 7
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 815 847 1230
Size: 815 32 383
Fragments arranged by size:
815 383 32
KasI G'GCGC_C
Cuts at: 0 197 1230
Size: 197 1033
MluI A'CGCG_T
Cuts at: 0 977 1230
Size: 977 253
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 62 1230
Size: 62 1168
MslI CAynn'nnrTG
Cuts at: 0 505 1183 1230
Size: 505 678 47
Fragments arranged by size:
678 505 47
MspA1I CmG'CkG
Cuts at: 0 18 27 326 1054 1230
Size: 18 9 299 728 176
Fragments arranged by size:
728 299 176 18 9
NarI GG'CG_CC
Cuts at: 0 198 1230
Size: 198 1032
NcoI C'CATG_G
Cuts at: 0 506 1230
Size: 506 724
NruI TCG'CGA
Cuts at: 0 573 1230
Size: 573 657
PasI CC'CwG_GG
Cuts at: 0 299 1230
Size: 299 931
PflMI CCAn_nnn'nTGG
Cuts at: 0 699 1230
Size: 699 531
PfoI T'CCnGG_A
Cuts at: 0 1106 1230
Size: 1106 124
PmlI CAC'GTG
Cuts at: 0 165 213 1230
Size: 165 48 1017
Fragments arranged by size:
1017 165 48
PvuII CAG'CTG
Cuts at: 0 1054 1230
Size: 1054 176
SacI G_AGCT'C
Cuts at: 0 272 1230
Size: 272 958
SacII CC_GC'GG
Cuts at: 0 19 28 327 1230
Size: 19 9 299 903
Fragments arranged by size:
903 299 19 9
SfcI C'TryA_G
Cuts at: 0 273 1230
Size: 273 957
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 27 1230
Size: 27 1203
SfoI GGC'GCC
Cuts at: 0 199 1230
Size: 199 1031
SmaI CCC'GGG
Cuts at: 0 44 737 1230
Size: 44 693 493
Fragments arranged by size:
693 493 44
SmlI C'TyrA_G
Cuts at: 0 538 1230
Size: 538 692
SnaBI TAC'GTA
Cuts at: 0 966 1230
Size: 966 264
StuI AGG'CCT
Cuts at: 0 383 1230
Size: 383 847
StyI C'CwwG_G
Cuts at: 0 92 506 653 1230
Size: 92 414 147 577
Fragments arranged by size:
577 414 147 92
TatI w'GTAC_w
Cuts at: 0 956 995 1230
Size: 956 39 235
Fragments arranged by size:
956 235 39
XhoI C'TCGA_G
Cuts at: 0 538 1230
Size: 538 692
XmaI C'CCGG_G
Cuts at: 0 42 735 1230
Size: 42 693 495
Fragments arranged by size:
693 495 42
Enzymes that do cut:
AarI AcuI AfeI AflIII AlwNI ApaLI AvaI BamHI
BanI BanII BbeI BbsI BbvCI BciVI BglI BlpI
Bme1580I BmgBI BplI BpmI Bpu10I BsaI BsaAI BsaHI
BsaXI BseRI BseYI BsiHKAI BsmBI Bsp1286I BspMI BsrBI
BsrDI BsrFI BsrGI BssSI BstAPI BstEII BstXI BstYI
BtgI DraIII EaeI EarI EciI EcoICRI Eco57MI FalI
HaeII Hin4I KasI MluI MmeI MslI MspA1I NarI
NcoI NruI PasI PflMI PfoI PmlI PvuII SacI
SacII SfcI SfiI SfoI SmaI SmlI SnaBI StuI
StyI TatI XhoI XmaI
Enzymes that do not cut:
AatII AccI Acc65I AclI AflII AgeI AhdI AleI
AlfI AloI ApaI ApoI AscI AseI AsiSI AvrII
BaeI BcgI BclI BfrBI BglII BmrI BmtI BpuEI
BsaBI BsaWI BsgI BsiEI BsiWI BsmI BspEI BspHI
BssHII BstBI BstZ17I Bsu36I BtgZI BtsI ClaI CspCI
DraI DrdI EagI EcoNI EcoO109I EcoRI EcoRV FseI
FspI FspAI HincII HindIII HpaI KpnI MfeI MscI
NaeI NdeI NgoMIV NheI NotI NsiI NspI PacI
PciI PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI
PsrI PstI PvuI RsrII SalI SanDI SapI SbfI
ScaI SexAI SgfI SgrAI SpeI SphI SrfI SspI
SwaI TaqII TstI Tth111I XbaI XcmI XmnI ZraI