Restriction map for
pFN21AE9525
(Linear) (Six-base) MAPSORT of: KIEE9525.seq Check: 3017 from: 1 to: 1092
KIEE9525.orf
With 182 enzymes: SgfI *
December 27, 2011 16:41 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 685 1092
Size: 685 407
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 616 1092
Size: 616 476
AflIII A'CryG_T
Cuts at: 0 617 1092
Size: 617 475
AlwNI CAG_nnn'CTG
Cuts at: 0 173 581 1092
Size: 173 408 511
Fragments arranged by size:
511 408 173
AvaI C'yCGr_G
Cuts at: 0 640 1092
Size: 640 452
BamHI G'GATC_C
Cuts at: 0 269 1092
Size: 269 823
BbsI GAAGACnn'nnnn_
Cuts at: 0 195 604 1092
Size: 195 409 488
Fragments arranged by size:
488 409 195
BglI GCCn_nnn'nGGC
Cuts at: 0 502 919 1092
Size: 502 417 173
Fragments arranged by size:
502 417 173
BmgBI CAC'GTC
Cuts at: 0 618 1092
Size: 618 474
BmrI ACTGGGnnnn_n'
Cuts at: 0 63 1092
Size: 63 1029
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 403 1026 1092
Size: 403 623 66
Fragments arranged by size:
623 403 66
BsaI GGTCTCn'nnnn_
Cuts at: 0 298 348 424 431 1068 1092
Size: 298 50 76 7 637 24
Fragments arranged by size:
637 298 76 50 24 7
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 85 115 382 412 1092
Size: 85 30 267 30 680
Fragments arranged by size:
680 267 85 30 30
BseYI C'CCAG_C
Cuts at: 0 5 360 839 1092
Size: 5 355 479 253
Fragments arranged by size:
479 355 253 5
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 667 1092
Size: 667 425
BsiEI CG_ry'CG
Cuts at: 0 290 1092
Size: 290 802
BsmBI CGTCTCn'nnnn_
Cuts at: 0 402 1092
Size: 402 690
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 566 685 1092
Size: 566 119 407
Fragments arranged by size:
566 407 119
BsrBI CCG'CTC
Cuts at: 0 742 1092
Size: 742 350
BsrDI GCAATG_nn'
Cuts at: 0 486 515 1092
Size: 486 29 577
Fragments arranged by size:
577 486 29
BsrFI r'CCGG_y
Cuts at: 0 918 977 1092
Size: 918 59 115
Fragments arranged by size:
918 115 59
BstAPI GCAn_nnn'nTGC
Cuts at: 0 868 1092
Size: 868 224
BstBI TT'CG_AA
Cuts at: 0 119 987 1092
Size: 119 868 105
Fragments arranged by size:
868 119 105
BstEII G'GTnAC_C
Cuts at: 0 672 1092
Size: 672 420
BstXI CCAn_nnnn'nTGG
Cuts at: 0 346 1092
Size: 346 746
BstYI r'GATC_y
Cuts at: 0 269 530 1092
Size: 269 261 562
Fragments arranged by size:
562 269 261
BtgI C'CryG_G
Cuts at: 0 588 1092
Size: 588 504
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 123 297 1092
Size: 123 174 795
Fragments arranged by size:
795 174 123
BtsI GCAGTG_nn'
Cuts at: 0 16 191 1092
Size: 16 175 901
Fragments arranged by size:
901 175 16
EaeI y'GGCC_r
Cuts at: 0 227 431 503 731 1092
Size: 227 204 72 228 361
Fragments arranged by size:
361 228 227 204 72
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 403 616 1026 1092
Size: 403 213 410 66
Fragments arranged by size:
410 403 213 66
EcoNI CCTnn'n_nnAGG
Cuts at: 0 447 1092
Size: 447 645
EcoO109I rG'GnC_Cy
Cuts at: 0 112 844 1092
Size: 112 732 248
Fragments arranged by size:
732 248 112
HaeII r_GCGC'y
Cuts at: 0 1049 1092
Size: 1049 43
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 402 434 754 786 1015 1047 1051 1083
Size: 402 32 320 32 229 32 4 32
Cuts at: 1083 1092
Size: 9
Fragments arranged by size:
402 320 229 32 32 32 32 9
4
HincII GTy'rAC
Cuts at: 0 1023 1092
Size: 1023 69
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 550 1092
Size: 550 542
MscI TGG'CCA
Cuts at: 0 433 505 733 1092
Size: 433 72 228 359
Fragments arranged by size:
433 359 228 72
MslI CAynn'nnrTG
Cuts at: 0 808 1092
Size: 808 284
MspA1I CmG'CkG
Cuts at: 0 261 626 1092
Size: 261 365 466
Fragments arranged by size:
466 365 261
NaeI GCC'GGC
Cuts at: 0 920 1092
Size: 920 172
NgoMIV G'CCGG_C
Cuts at: 0 918 1092
Size: 918 174
NspI r_CATG'y
Cuts at: 0 866 1092
Size: 866 226
PflMI CCAn_nnn'nTGG
Cuts at: 0 173 1092
Size: 173 919
PfoI T'CCnGG_A
Cuts at: 0 380 526 1092
Size: 380 146 566
Fragments arranged by size:
566 380 146
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 328 360 1092
Size: 328 32 732
Fragments arranged by size:
732 328 32
PpuMI rG'GwC_Cy
Cuts at: 0 844 1092
Size: 844 248
SanDI GG'GwC_CC
Cuts at: 0 844 1092
Size: 844 248
SexAI A'CCwGG_T
Cuts at: 0 338 1092
Size: 338 754
SmaI CCC'GGG
Cuts at: 0 642 1092
Size: 642 450
SphI G_CATG'C
Cuts at: 0 866 1092
Size: 866 226
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 82 141 790 1092
Size: 82 59 649 302
Fragments arranged by size:
649 302 82 59
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 741 1092
Size: 741 351
XmaI C'CCGG_G
Cuts at: 0 640 1092
Size: 640 452
Enzymes that do cut:
AarI AcuI AflIII AlwNI AvaI BamHI BbsI BglI
BmgBI BmrI BpmI BsaI BsaXI BseYI BsgI BsiEI
BsmBI BspMI BsrBI BsrDI BsrFI BstAPI BstBI BstEII
BstXI BstYI BtgI BtgZI BtsI EaeI Eco57MI EcoNI
EcoO109I HaeII Hin4I HincII MmeI MscI MslI MspA1I
NaeI NgoMIV NspI PflMI PfoI PpiI PpuMI SanDI
SexAI SmaI SphI TaqII XcmI XmaI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AgeI AhdI
AleI AlfI AloI ApaI ApaLI ApoI AscI AseI
AsiSI AvrII BaeI BanI BanII BbeI BbvCI BcgI
BciVI BclI BfrBI BglII BlpI Bme1580I BmtI BplI
Bpu10I BpuEI BsaAI BsaBI BsaHI BsaWI BseRI BsiHKAI
BsiWI BsmI Bsp1286I BspEI BspHI BsrGI BssHII BssSI
BstZ17I Bsu36I ClaI CspCI DraI DraIII DrdI EagI
EarI EciI EcoICRI EcoRI EcoRV FalI FseI FspI
FspAI HindIII HpaI KasI KpnI MfeI MluI NarI
NcoI NdeI NheI NotI NruI NsiI PacI PasI
PciI PmeI PmlI PshAI PsiI PspOMI PspXI PsrI
PstI PvuI PvuII RsrII SacI SacII SalI SapI
SbfI ScaI SfcI SfiI SfoI SgfI SgrAI SmlI
SnaBI SpeI SrfI SspI StuI StyI SwaI TatI
TstI Tth111I XbaI XhoI XmnI ZraI