(Linear) (Six-base) MAPSORT of: KIEE9523.seq  Check: 8726  from: 1  to: 1383



KIEE9523.orf

 With 182 enzymes: SgfI * 

                        December 27, 2011 16:41  ..

AccI GT'mk_AC

Cuts at:      0     804    1383 
   Size:        804     579
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1088    1383 
   Size:       1088     295
 
AflIII A'CryG_T

Cuts at:      0     939    1383 
   Size:        939     444
 
AleI CACnn'nnGTG

Cuts at:      0     589    1383 
   Size:        589     794
 
AlwNI CAG_nnn'CTG

Cuts at:      0     203     244    1383 
   Size:        203      41    1139

  Fragments arranged by size: 

                1139     203      41
 
ApoI r'AATT_y

Cuts at:      0     417    1042    1371    1383 
   Size:        417     625     329      12

  Fragments arranged by size: 

                 625     417     329      12
 
BanI G'GyrC_C

Cuts at:      0    1023    1383 
   Size:       1023     360
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     412    1383 
   Size:        412     971
 
BglII A'GATC_T

Cuts at:      0     308    1383 
   Size:        308    1075
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     369     639    1383 
   Size:        369     270     744

  Fragments arranged by size: 

                 744     369     270
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1028    1383 
   Size:       1028     355
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    1143    1383 
   Size:       1143     240
 
BsaBI GATnn'nnATC

Cuts at:      0     863    1383 
   Size:        863     520
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0      25    1383 
   Size:         25    1358
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     859    1099    1383 
   Size:        859     240     284

  Fragments arranged by size: 

                 859     284     240
 
BseYI C'CCAG_C

Cuts at:      0     776    1383 
   Size:        776     607
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     395    1383 
   Size:        395     988
 
BspHI T'CATG_A

Cuts at:      0     122    1299    1383 
   Size:        122    1177      84

  Fragments arranged by size: 

                1177     122      84
 
BsrDI GCAATG_nn'

Cuts at:      0     812    1027    1383 
   Size:        812     215     356

  Fragments arranged by size: 

                 812     356     215
 
BsrGI T'GTAC_A

Cuts at:      0     989    1383 
   Size:        989     394
 
BssSI C'ACGA_G

Cuts at:      0     394    1383 
   Size:        394     989
 
BstYI r'GATC_y

Cuts at:      0     308    1383 
   Size:        308    1075
 
BstZ17I GTA'TAC

Cuts at:      0     805    1383 
   Size:        805     578
 
Bsu36I CC'TnA_GG

Cuts at:      0      83    1383 
   Size:         83    1300
 
BtgI C'CryG_G

Cuts at:      0     882    1057    1383 
   Size:        882     175     326

  Fragments arranged by size: 

                 882     326     175
 
BtsI GCAGTG_nn'

Cuts at:      0     463     584     596    1383 
   Size:        463     121      12     787

  Fragments arranged by size: 

                 787     463     121      12
 
EaeI y'GGCC_r

Cuts at:      0     593     728    1383 
   Size:        593     135     655

  Fragments arranged by size: 

                 655     593     135
 
EarI CTCTTCn'nnn_

Cuts at:      0      68    1250    1383 
   Size:         68    1182     133

  Fragments arranged by size: 

                1182     133      68
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1088    1383 
   Size:       1088     295
 
HincII GTy'rAC

Cuts at:      0     387    1271    1383 
   Size:        387     884     112

  Fragments arranged by size: 

                 884     387     112
 
HindIII A'AGCT_T

Cuts at:      0     822    1383 
   Size:        822     561
 
HpaI GTT'AAC

Cuts at:      0     387    1383 
   Size:        387     996
 
MfeI C'AATT_G

Cuts at:      0     840    1383 
   Size:        840     543
 
MluI A'CGCG_T

Cuts at:      0     939    1383 
   Size:        939     444
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    1256    1383 
   Size:       1256     127
 
MscI TGG'CCA

Cuts at:      0     595    1383 
   Size:        595     788
 
MslI CAynn'nnrTG

Cuts at:      0     589     724    1114    1322    1383 
   Size:        589     135     390     208      61

  Fragments arranged by size: 

                 589     390     208     135      61
 
NcoI C'CATG_G

Cuts at:      0     882    1057    1383 
   Size:        882     175     326

  Fragments arranged by size: 

                 882     326     175
 
NspI r_CATG'y

Cuts at:      0     811    1383 
   Size:        811     572
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    1033    1383 
   Size:       1033     350
 
PstI C_TGCA'G

Cuts at:      0     591    1383 
   Size:        591     792
 
SapI GCTCTTCn'nnn_

Cuts at:      0    1250    1383 
   Size:       1250     133
 
SfcI C'TryA_G

Cuts at:      0     587    1383 
   Size:        587     796
 
SspI AAT'ATT

Cuts at:      0     933    1383 
   Size:        933     450
 
StuI AGG'CCT

Cuts at:      0     607    1383 
   Size:        607     776
 
StyI C'CwwG_G

Cuts at:      0     882    1057    1383 
   Size:        882     175     326

  Fragments arranged by size: 

                 882     326     175
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     406    1383 
   Size:        406     977
 
TatI w'GTAC_w

Cuts at:      0     989    1383 
   Size:        989     394
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0      27     678     710    1383 
   Size:         27     651      32     673

  Fragments arranged by size: 

                 673     651      32      27
 
XmnI GAAnn'nnTTC

Cuts at:      0    1244    1352    1383 
   Size:       1244     108      31

  Fragments arranged by size: 

                1244     108      31
 

 Enzymes that do cut:

     AccI     AcuI   AflIII     AleI    AlwNI     ApoI     BanI     BbsI
    BglII     BmrI     BplI     BsaI    BsaBI    BsaXI    BseRI    BseYI
     BsgI    BspHI    BsrDI    BsrGI    BssSI    BstYI  BstZ17I   Bsu36I
     BtgI     BtsI     EaeI     EarI  Eco57MI   HincII  HindIII     HpaI
     MfeI     MluI     MmeI     MscI     MslI     NcoI     NspI    PflMI
     PstI     SapI     SfcI     SspI     StuI     StyI    TaqII     TatI
     TstI     XmnI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AfeI    AflII     AgeI     AhdI
     AlfI     AloI     ApaI    ApaLI     AscI     AseI    AsiSI     AvaI
    AvrII     BaeI    BamHI    BanII     BbeI    BbvCI     BcgI    BciVI
     BclI    BfrBI     BglI     BlpI Bme1580I    BmgBI     BmtI     BpmI
   Bpu10I    BpuEI    BsaAI    BsaHI    BsaWI    BsiEI  BsiHKAI    BsiWI
     BsmI    BsmBI Bsp1286I    BspEI    BspMI    BsrBI    BsrFI   BssHII
   BstAPI    BstBI   BstEII    BstXI    BtgZI     ClaI    CspCI     DraI
   DraIII     DrdI     EagI     EciI  EcoICRI    EcoNI EcoO109I    EcoRI
    EcoRV     FalI     FseI     FspI    FspAI    HaeII    Hin4I     KasI
     KpnI   MspA1I     NaeI     NarI     NdeI   NgoMIV     NheI     NotI
     NruI     NsiI     PacI     PasI     PciI     PfoI     PmeI     PmlI
     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI     PvuI
    PvuII    RsrII     SacI    SacII     SalI    SanDI     SbfI     ScaI
    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI
     SpeI     SphI     SrfI     SwaI  Tth111I     XbaI     XcmI     XhoI
     XmaI     ZraI