Restriction map for
pFN21AE9521
(Linear) (Six-base) MAPSORT of: KIEE9521.seq Check: 1507 from: 1 to: 951
KIEE9521.orf
With 182 enzymes: SgfI *
January 29, 2014 12:07 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 379 951
Size: 379 572
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 507 951
Size: 507 444
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 397 951
Size: 397 554
AlwNI CAG_nnn'CTG
Cuts at: 0 345 395 951
Size: 345 50 556
Fragments arranged by size:
556 345 50
ApaI G_GGCC'C
Cuts at: 0 766 951
Size: 766 185
ApaLI G'TGCA_C
Cuts at: 0 311 951
Size: 311 640
AvaI C'yCGr_G
Cuts at: 0 323 635 802 951
Size: 323 312 167 149
Fragments arranged by size:
323 312 167 149
BamHI G'GATC_C
Cuts at: 0 13 951
Size: 13 938
BanI G'GyrC_C
Cuts at: 0 101 406 951
Size: 101 305 545
Fragments arranged by size:
545 305 101
BanII G_rGCy'C
Cuts at: 0 37 99 131 766 914 951
Size: 37 62 32 635 148 37
Fragments arranged by size:
635 148 62 37 37 32
BbeI G_GCGC'C
Cuts at: 0 410 951
Size: 410 541
BbsI GAAGACnn'nnnn_
Cuts at: 0 30 513 573 951
Size: 30 483 60 378
Fragments arranged by size:
483 378 60 30
BbvCI CC'TCA_GC
Cuts at: 0 136 951
Size: 136 815
BclI T'GATC_A
Cuts at: 0 365 951
Size: 365 586
BglI GCCn_nnn'nGGC
Cuts at: 0 496 713 951
Size: 496 217 238
Fragments arranged by size:
496 238 217
Bme1580I G_kGCm'C
Cuts at: 0 315 766 951
Size: 315 451 185
Fragments arranged by size:
451 315 185
BmgBI CAC'GTC
Cuts at: 0 363 951
Size: 363 588
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 127 298 322 951
Size: 127 171 24 629
Fragments arranged by size:
629 171 127 24
Bpu10I CC'TnA_GC
Cuts at: 0 136 951
Size: 136 815
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 910 951
Size: 910 41
BsaAI yAC'GTr
Cuts at: 0 555 815 951
Size: 555 260 136
Fragments arranged by size:
555 260 136
BsaHI Gr'CG_yC
Cuts at: 0 407 951
Size: 407 544
BsaWI w'CCGG_w
Cuts at: 0 197 951
Size: 197 754
BseYI C'CCAG_C
Cuts at: 0 65 69 738 758 951
Size: 65 4 669 20 193
Fragments arranged by size:
669 193 65 20 4
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 28 190 951
Size: 28 162 761
Fragments arranged by size:
761 162 28
BsiEI CG_ry'CG
Cuts at: 0 287 951
Size: 287 664
BsiHKAI G_wGCw'C
Cuts at: 0 315 605 809 914 951
Size: 315 290 204 105 37
Fragments arranged by size:
315 290 204 105 37
BsmBI CGTCTCn'nnnn_
Cuts at: 0 274 951
Size: 274 677
Bsp1286I G_dGCh'C
Cuts at: 0 37 99 131 315 605 766 809 914
Size: 37 62 32 184 290 161 43 105
Cuts at: 914 951
Size: 37
Fragments arranged by size:
290 184 161 105 62 43 37 37
32
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 212 260 379 951
Size: 212 48 119 572
Fragments arranged by size:
572 212 119 48
BsrBI CCG'CTC
Cuts at: 0 263 951
Size: 263 688
BsrDI GCAATG_nn'
Cuts at: 0 873 951
Size: 873 78
BsrFI r'CCGG_y
Cuts at: 0 307 951
Size: 307 644
BssSI C'ACGA_G
Cuts at: 0 574 951
Size: 574 377
BstXI CCAn_nnnn'nTGG
Cuts at: 0 417 507 703 738 951
Size: 417 90 196 35 213
Fragments arranged by size:
417 213 196 90 35
BstYI r'GATC_y
Cuts at: 0 13 951
Size: 13 938
BtgI C'CryG_G
Cuts at: 0 416 475 951
Size: 416 59 476
Fragments arranged by size:
476 416 59
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 171 396 522 645 951
Size: 171 225 126 123 306
Fragments arranged by size:
306 225 171 126 123
BtsI GCAGTG_nn'
Cuts at: 0 445 951
Size: 445 506
EaeI y'GGCC_r
Cuts at: 0 179 284 497 509 557 608 951
Size: 179 105 213 12 48 51 343
Fragments arranged by size:
343 213 179 105 51 48 12
EagI C'GGCC_G
Cuts at: 0 284 951
Size: 284 667
EarI CTCTTCn'nnn_
Cuts at: 0 136 538 951
Size: 136 402 413
Fragments arranged by size:
413 402 136
EcoICRI GAG'CTC
Cuts at: 0 912 951
Size: 912 39
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 127 298 322 507 951
Size: 127 171 24 185 444
Fragments arranged by size:
444 185 171 127 24
EcoNI CCTnn'n_nnAGG
Cuts at: 0 301 951
Size: 301 650
EcoO109I rG'GnC_Cy
Cuts at: 0 762 763 951
Size: 762 1 188
Fragments arranged by size:
762 188 1
HaeII r_GCGC'y
Cuts at: 0 410 687 719 951
Size: 410 277 32 232
Fragments arranged by size:
410 277 232 32
KasI G'GCGC_C
Cuts at: 0 406 951
Size: 406 545
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 227 951
Size: 227 724
MscI TGG'CCA
Cuts at: 0 181 511 951
Size: 181 330 440
Fragments arranged by size:
440 330 181
MslI CAynn'nnrTG
Cuts at: 0 361 415 505 610 951
Size: 361 54 90 105 341
Fragments arranged by size:
361 341 105 90 54
MspA1I CmG'CkG
Cuts at: 0 69 345 477 951
Size: 69 276 132 474
Fragments arranged by size:
474 276 132 69
NarI GG'CG_CC
Cuts at: 0 407 951
Size: 407 544
NspI r_CATG'y
Cuts at: 0 630 944 951
Size: 630 314 7
Fragments arranged by size:
630 314 7
PasI CC'CwG_GG
Cuts at: 0 654 951
Size: 654 297
PflMI CCAn_nnn'nTGG
Cuts at: 0 630 951
Size: 630 321
PmlI CAC'GTG
Cuts at: 0 555 815 951
Size: 555 260 136
Fragments arranged by size:
555 260 136
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 193 225 951
Size: 193 32 726
Fragments arranged by size:
726 193 32
PshAI GACnn'nnGTC
Cuts at: 0 286 951
Size: 286 665
PspOMI G'GGCC_C
Cuts at: 0 762 951
Size: 762 189
PstI C_TGCA'G
Cuts at: 0 341 376 951
Size: 341 35 575
Fragments arranged by size:
575 341 35
PvuII CAG'CTG
Cuts at: 0 69 345 951
Size: 69 276 606
Fragments arranged by size:
606 276 69
SacI G_AGCT'C
Cuts at: 0 914 951
Size: 914 37
SacII CC_GC'GG
Cuts at: 0 478 951
Size: 478 473
SapI GCTCTTCn'nnn_
Cuts at: 0 136 538 951
Size: 136 402 413
Fragments arranged by size:
413 402 136
SfcI C'TryA_G
Cuts at: 0 337 372 951
Size: 337 35 579
Fragments arranged by size:
579 337 35
SfoI GGC'GCC
Cuts at: 0 408 951
Size: 408 543
SmaI CCC'GGG
Cuts at: 0 325 637 951
Size: 325 312 314
Fragments arranged by size:
325 314 312
SmlI C'TyrA_G
Cuts at: 0 925 951
Size: 925 26
SrfI GCCC'GGGC
Cuts at: 0 325 637 951
Size: 325 312 314
Fragments arranged by size:
325 314 312
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 228 495 951
Size: 228 267 456
Fragments arranged by size:
456 267 228
TatI w'GTAC_w
Cuts at: 0 218 951
Size: 218 733
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 776 808 951
Size: 776 32 143
Fragments arranged by size:
776 143 32
Tth111I GACn'n_nGTC
Cuts at: 0 352 951
Size: 352 599
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 66 700 951
Size: 66 634 251
Fragments arranged by size:
634 251 66
XmaI C'CCGG_G
Cuts at: 0 323 635 951
Size: 323 312 316
Fragments arranged by size:
323 316 312
Enzymes that do cut:
AarI AcuI AlfI AlwNI ApaI ApaLI AvaI BamHI
BanI BanII BbeI BbsI BbvCI BclI BglI Bme1580I
BmgBI BpmI Bpu10I BpuEI BsaAI BsaHI BsaWI BseYI
BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspMI BsrBI BsrDI
BsrFI BssSI BstXI BstYI BtgI BtgZI BtsI EaeI
EagI EarI EcoICRI Eco57MI EcoNI EcoO109I HaeII KasI
MmeI MscI MslI MspA1I NarI NspI PasI PflMI
PmlI PpiI PshAI PspOMI PstI PvuII SacI SacII
SapI SfcI SfoI SmaI SmlI SrfI TaqII TatI
TstI Tth111I XcmI XmaI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AflIII AgeI
AhdI AleI AloI ApoI AscI AseI AsiSI AvrII
BaeI BcgI BciVI BfrBI BglII BlpI BmrI BmtI
BplI BsaI BsaBI BsaXI BseRI BsiWI BsmI BspEI
BspHI BsrGI BssHII BstAPI BstBI BstEII BstZ17I Bsu36I
ClaI CspCI DraI DraIII DrdI EciI EcoRI EcoRV
FalI FseI FspI FspAI Hin4I HincII HindIII HpaI
KpnI MfeI MluI NaeI NcoI NdeI NgoMIV NheI
NotI NruI NsiI PacI PciI PfoI PmeI PpuMI
PsiI PspXI PsrI PvuI RsrII SalI SanDI SbfI
ScaI SexAI SfiI SgfI SgrAI SnaBI SpeI SphI
SspI StuI StyI SwaI XbaI XhoI XmnI ZraI