Restriction map for
pFN21AE9520
(Linear) (Six-base) MAPSORT of: KIEE9520.seq Check: 6051 from: 1 to: 1335
KIEE9520.orf
With 182 enzymes: SgfI *
March 22, 2012 18:29 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 47 243 422 1335
Size: 47 196 179 913
Fragments arranged by size:
913 196 179 47
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 602 1335
Size: 602 733
AflIII A'CryG_T
Cuts at: 0 1292 1335
Size: 1292 43
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 295 387 1335
Size: 295 92 948
Fragments arranged by size:
948 295 92
AlwNI CAG_nnn'CTG
Cuts at: 0 258 625 830 1335
Size: 258 367 205 505
Fragments arranged by size:
505 367 258 205
AvaI C'yCGr_G
Cuts at: 0 508 1335
Size: 508 827
BanI G'GyrC_C
Cuts at: 0 296 535 775 1099 1335
Size: 296 239 240 324 236
Fragments arranged by size:
324 296 240 239 236
BanII G_rGCy'C
Cuts at: 0 67 711 1081 1246 1335
Size: 67 644 370 165 89
Fragments arranged by size:
644 370 165 89 67
BbsI GAAGACnn'nnnn_
Cuts at: 0 631 1335
Size: 631 704
BglI GCCn_nnn'nGGC
Cuts at: 0 283 313 839 1228 1335
Size: 283 30 526 389 107
Fragments arranged by size:
526 389 283 107 30
Bme1580I G_kGCm'C
Cuts at: 0 274 465 517 597 1102 1335
Size: 274 191 52 80 505 233
Fragments arranged by size:
505 274 233 191 80 52
BmrI ACTGGGnnnn_n'
Cuts at: 0 304 1335
Size: 304 1031
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 222 682 1335
Size: 222 460 653
Fragments arranged by size:
653 460 222
Bpu10I CC'TnA_GC
Cuts at: 0 1075 1335
Size: 1075 260
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 426 583 1335
Size: 426 157 752
Fragments arranged by size:
752 426 157
BsaAI yAC'GTr
Cuts at: 0 1164 1293 1335
Size: 1164 129 42
Fragments arranged by size:
1164 129 42
BsaHI Gr'CG_yC
Cuts at: 0 918 1335
Size: 918 417
BsaWI w'CCGG_w
Cuts at: 0 24 1335
Size: 24 1311
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 219 1335
Size: 219 1116
BseYI C'CCAG_C
Cuts at: 0 258 837 945 1014 1032 1335
Size: 258 579 108 69 18 303
Fragments arranged by size:
579 303 258 108 69 18
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 271 511 910 1017 1335
Size: 271 240 399 107 318
Fragments arranged by size:
399 318 271 240 107
BsiEI CG_ry'CG
Cuts at: 0 1207 1335
Size: 1207 128
BsmI GAATG_Cn'
Cuts at: 0 327 1335
Size: 327 1008
BsmBI CGTCTCn'nnnn_
Cuts at: 0 156 1335
Size: 156 1179
Bsp1286I G_dGCh'C
Cuts at: 0 67 274 465 517 597 711 1081 1102
Size: 67 207 191 52 80 114 370 21
Cuts at: 1102 1246 1335
Size: 144 89
Fragments arranged by size:
370 207 191 144 114 89 80 67
52 21
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 47 222 243 422 989 1335
Size: 47 175 21 179 567 346
Fragments arranged by size:
567 346 179 175 47 21
BsrBI CCG'CTC
Cuts at: 0 552 1335
Size: 552 783
BsrDI GCAATG_nn'
Cuts at: 0 1210 1335
Size: 1210 125
BsrFI r'CCGG_y
Cuts at: 0 1202 1206 1222 1335
Size: 1202 4 16 113
Fragments arranged by size:
1202 113 16 4
BssSI C'ACGA_G
Cuts at: 0 1120 1335
Size: 1120 215
BstAPI GCAn_nnn'nTGC
Cuts at: 0 376 601 830 1335
Size: 376 225 229 505
Fragments arranged by size:
505 376 229 225
BstEII G'GTnAC_C
Cuts at: 0 192 1335
Size: 192 1143
BstXI CCAn_nnnn'nTGG
Cuts at: 0 131 246 966 1239 1335
Size: 131 115 720 273 96
Fragments arranged by size:
720 273 131 115 96
BstYI r'GATC_y
Cuts at: 0 86 1335
Size: 86 1249
Bsu36I CC'TnA_GG
Cuts at: 0 797 1335
Size: 797 538
BtgI C'CryG_G
Cuts at: 0 983 1335
Size: 983 352
BtsI GCAGTG_nn'
Cuts at: 0 745 831 957 1335
Size: 745 86 126 378
Fragments arranged by size:
745 378 126 86
DraIII CAC_nnn'GTG
Cuts at: 0 303 1335
Size: 303 1032
DrdI GACnn_nn'nnGTC
Cuts at: 0 232 1335
Size: 232 1103
EaeI y'GGCC_r
Cuts at: 0 848 980 1204 1229 1335
Size: 848 132 224 25 106
Fragments arranged by size:
848 224 132 106 25
EagI C'GGCC_G
Cuts at: 0 1204 1335
Size: 1204 131
EarI CTCTTCn'nnn_
Cuts at: 0 58 719 728 1335
Size: 58 661 9 607
Fragments arranged by size:
661 607 58 9
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 222 602 682 1335
Size: 222 380 80 653
Fragments arranged by size:
653 380 222 80
EcoNI CCTnn'n_nnAGG
Cuts at: 0 490 1335
Size: 490 845
EcoO109I rG'GnC_Cy
Cuts at: 0 234 1001 1335
Size: 234 767 334
Fragments arranged by size:
767 334 234
FseI GG_CCGG'CC
Cuts at: 0 1206 1335
Size: 1206 129
HaeII r_GCGC'y
Cuts at: 0 1066 1259 1335
Size: 1066 193 76
Fragments arranged by size:
1066 193 76
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 673 680 705 712 1335
Size: 673 7 25 7 623
Fragments arranged by size:
673 623 25 7 7
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 112 1335
Size: 112 1223
MscI TGG'CCA
Cuts at: 0 982 1231 1335
Size: 982 249 104
Fragments arranged by size:
982 249 104
MslI CAynn'nnrTG
Cuts at: 0 244 369 988 1237 1335
Size: 244 125 619 249 98
Fragments arranged by size:
619 249 244 125 98
MspA1I CmG'CkG
Cuts at: 0 622 1335
Size: 622 713
NaeI GCC'GGC
Cuts at: 0 1204 1208 1224 1335
Size: 1204 4 16 111
Fragments arranged by size:
1204 111 16 4
NcoI C'CATG_G
Cuts at: 0 983 1335
Size: 983 352
NgoMIV G'CCGG_C
Cuts at: 0 1202 1206 1222 1335
Size: 1202 4 16 113
Fragments arranged by size:
1202 113 16 4
NspI r_CATG'y
Cuts at: 0 374 1335
Size: 374 961
PflMI CCAn_nnn'nTGG
Cuts at: 0 42 1335
Size: 42 1293
PmlI CAC'GTG
Cuts at: 0 1293 1335
Size: 1293 42
PpuMI rG'GwC_Cy
Cuts at: 0 234 1001 1335
Size: 234 767 334
Fragments arranged by size:
767 334 234
PspXI vC'TCGA_Gb
Cuts at: 0 508 1335
Size: 508 827
PvuII CAG'CTG
Cuts at: 0 622 1335
Size: 622 713
SmlI C'TyrA_G
Cuts at: 0 405 508 562 1335
Size: 405 103 54 773
Fragments arranged by size:
773 405 103 54
SphI G_CATG'C
Cuts at: 0 374 1335
Size: 374 961
StuI AGG'CCT
Cuts at: 0 455 695 802 1335
Size: 455 240 107 533
Fragments arranged by size:
533 455 240 107
StyI C'CwwG_G
Cuts at: 0 983 1004 1335
Size: 983 21 331
Fragments arranged by size:
983 331 21
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 24 81 976 1125 1335
Size: 24 57 895 149 210
Fragments arranged by size:
895 210 149 57 24
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 771 803 1335
Size: 771 32 532
Fragments arranged by size:
771 532 32
XhoI C'TCGA_G
Cuts at: 0 508 1335
Size: 508 827
Enzymes that do cut:
AarI AcuI AflIII AlfI AlwNI AvaI BanI BanII
BbsI BglI Bme1580I BmrI BpmI Bpu10I BpuEI BsaAI
BsaHI BsaWI BseRI BseYI BsgI BsiEI BsmI BsmBI
Bsp1286I BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstEII
BstXI BstYI Bsu36I BtgI BtsI DraIII DrdI EaeI
EagI EarI Eco57MI EcoNI EcoO109I FseI HaeII Hin4I
MmeI MscI MslI MspA1I NaeI NcoI NgoMIV NspI
PflMI PmlI PpuMI PspXI PvuII SmlI SphI StuI
StyI TaqII TstI XhoI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AgeI AhdI
AleI AloI ApaI ApaLI ApoI AscI AseI AsiSI
AvrII BaeI BamHI BbeI BbvCI BcgI BciVI BclI
BfrBI BglII BlpI BmgBI BmtI BplI BsaI BsaBI
BsaXI BsiHKAI BsiWI BspEI BspHI BsrGI BssHII BstBI
BstZ17I BtgZI ClaI CspCI DraI EciI EcoICRI EcoRI
EcoRV FalI FspI FspAI HincII HindIII HpaI KasI
KpnI MfeI MluI NarI NdeI NheI NotI NruI
NsiI PacI PasI PciI PfoI PmeI PpiI PshAI
PsiI PspOMI PsrI PstI PvuI RsrII SacI SacII
SalI SanDI SapI SbfI ScaI SexAI SfcI SfiI
SfoI SgfI SgrAI SmaI SnaBI SpeI SrfI SspI
SwaI TatI Tth111I XbaI XcmI XmaI XmnI ZraI