Restriction map for
pFN21AE9519
(Linear) (Six-base) MAPSORT of: KIEE9519.seq Check: 819 from: 1 to: 1074
KIEE9519.orf
With 182 enzymes: SgfI *
July 3, 2012 19:02 ..
AccI GT'mk_AC
Cuts at: 0 473 1074
Size: 473 601
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 819 1074
Size: 819 255
AfeI AGC'GCT
Cuts at: 0 355 752 1074
Size: 355 397 322
Fragments arranged by size:
397 355 322
AflIII A'CryG_T
Cuts at: 0 296 1074
Size: 296 778
AleI CACnn'nnGTG
Cuts at: 0 178 1074
Size: 178 896
ApaI G_GGCC'C
Cuts at: 0 714 1074
Size: 714 360
AvaI C'yCGr_G
Cuts at: 0 890 941 1032 1074
Size: 890 51 91 42
Fragments arranged by size:
890 91 51 42
BanI G'GyrC_C
Cuts at: 0 384 628 1074
Size: 384 244 446
Fragments arranged by size:
446 384 244
BanII G_rGCy'C
Cuts at: 0 643 714 980 1074
Size: 643 71 266 94
Fragments arranged by size:
643 266 94 71
BbeI G_GCGC'C
Cuts at: 0 388 632 1074
Size: 388 244 442
Fragments arranged by size:
442 388 244
BbsI GAAGACnn'nnnn_
Cuts at: 0 55 858 1074
Size: 55 803 216
Fragments arranged by size:
803 216 55
BbvCI CC'TCA_GC
Cuts at: 0 992 1074
Size: 992 82
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 99 133 1074
Size: 99 34 941
Fragments arranged by size:
941 99 34
BglI GCCn_nnn'nGGC
Cuts at: 0 275 463 799 1074
Size: 275 188 336 275
Fragments arranged by size:
336 275 275 188
BglII A'GATC_T
Cuts at: 0 879 1074
Size: 879 195
BlpI GC'TnA_GC
Cuts at: 0 40 307 343 1074
Size: 40 267 36 731
Fragments arranged by size:
731 267 40 36
Bme1580I G_kGCm'C
Cuts at: 0 714 1074
Size: 714 360
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 571 896 1074
Size: 571 325 178
Fragments arranged by size:
571 325 178
Bpu10I CC'TnA_GC
Cuts at: 0 544 992 1074
Size: 544 448 82
Fragments arranged by size:
544 448 82
BsaI GGTCTCn'nnnn_
Cuts at: 0 1022 1074
Size: 1022 52
BsaAI yAC'GTr
Cuts at: 0 297 1074
Size: 297 777
BsaHI Gr'CG_yC
Cuts at: 0 385 629 1074
Size: 385 244 445
Fragments arranged by size:
445 385 244
BsaWI w'CCGG_w
Cuts at: 0 762 1059 1074
Size: 762 297 15
Fragments arranged by size:
762 297 15
BseYI C'CCAG_C
Cuts at: 0 96 264 635 783 1074
Size: 96 168 371 148 291
Fragments arranged by size:
371 291 168 148 96
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 134 169 1074
Size: 134 35 905
Fragments arranged by size:
905 134 35
BsiEI CG_ry'CG
Cuts at: 0 163 467 746 954 1074
Size: 163 304 279 208 120
Fragments arranged by size:
304 279 208 163 120
BsiHKAI G_wGCw'C
Cuts at: 0 860 980 1074
Size: 860 120 94
Fragments arranged by size:
860 120 94
Bsp1286I G_dGCh'C
Cuts at: 0 643 714 860 980 1074
Size: 643 71 146 120 94
Fragments arranged by size:
643 146 120 94 71
BspEI T'CCGG_A
Cuts at: 0 1059 1074
Size: 1059 15
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 886 1074
Size: 886 188
BsrBI CCG'CTC
Cuts at: 0 228 811 956 1074
Size: 228 583 145 118
Fragments arranged by size:
583 228 145 118
BsrDI GCAATG_nn'
Cuts at: 0 1029 1074
Size: 1029 45
BsrFI r'CCGG_y
Cuts at: 0 334 522 668 793 1074
Size: 334 188 146 125 281
Fragments arranged by size:
334 281 188 146 125
BssHII G'CGCG_C
Cuts at: 0 302 1074
Size: 302 772
BstBI TT'CG_AA
Cuts at: 0 923 1074
Size: 923 151
BstYI r'GATC_y
Cuts at: 0 879 1074
Size: 879 195
BtgI C'CryG_G
Cuts at: 0 971 1017 1074
Size: 971 46 57
Fragments arranged by size:
971 57 46
BtsI GCAGTG_nn'
Cuts at: 0 34 1074
Size: 34 1040
EaeI y'GGCC_r
Cuts at: 0 170 197 259 440 464 795 951 1074
Size: 170 27 62 181 24 331 156 123
Fragments arranged by size:
331 181 170 156 123 62 27 24
EagI C'GGCC_G
Cuts at: 0 464 951 1074
Size: 464 487 123
Fragments arranged by size:
487 464 123
EarI CTCTTCn'nnn_
Cuts at: 0 925 1074
Size: 925 149
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 803 1074
Size: 803 271
EcoICRI GAG'CTC
Cuts at: 0 978 1074
Size: 978 96
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 571 819 896 1074
Size: 571 248 77 178
Fragments arranged by size:
571 248 178 77
EcoNI CCTnn'n_nnAGG
Cuts at: 0 736 1074
Size: 736 338
EcoO109I rG'GnC_Cy
Cuts at: 0 711 770 898 1068 1074
Size: 711 59 128 170 6
Fragments arranged by size:
711 170 128 59 6
FspI TGC'GCA
Cuts at: 0 397 1074
Size: 397 677
HaeII r_GCGC'y
Cuts at: 0 94 196 357 388 632 683 754 1074
Size: 94 102 161 31 244 51 71 320
Fragments arranged by size:
320 244 161 102 94 71 51 31
HincII GTy'rAC
Cuts at: 0 729 849 1074
Size: 729 120 225
Fragments arranged by size:
729 225 120
KasI G'GCGC_C
Cuts at: 0 384 628 1074
Size: 384 244 446
Fragments arranged by size:
446 384 244
MslI CAynn'nnrTG
Cuts at: 0 178 1074
Size: 178 896
MspA1I CmG'CkG
Cuts at: 0 165 264 507 635 874 996 1019 1074
Size: 165 99 243 128 239 122 23 55
Fragments arranged by size:
243 239 165 128 122 99 55 23
NaeI GCC'GGC
Cuts at: 0 670 795 1074
Size: 670 125 279
Fragments arranged by size:
670 279 125
NarI GG'CG_CC
Cuts at: 0 385 629 1074
Size: 385 244 445
Fragments arranged by size:
445 385 244
NcoI C'CATG_G
Cuts at: 0 971 1074
Size: 971 103
NgoMIV G'CCGG_C
Cuts at: 0 668 793 1074
Size: 668 125 281
Fragments arranged by size:
668 281 125
NotI GC'GGCC_GC
Cuts at: 0 951 1074
Size: 951 123
NspI r_CATG'y
Cuts at: 0 315 1074
Size: 315 759
PasI CC'CwG_GG
Cuts at: 0 22 1074
Size: 22 1052
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 975 1007 1074
Size: 975 32 67
Fragments arranged by size:
975 67 32
PpuMI rG'GwC_Cy
Cuts at: 0 898 1068 1074
Size: 898 170 6
Fragments arranged by size:
898 170 6
PspOMI G'GGCC_C
Cuts at: 0 710 1074
Size: 710 364
PstI C_TGCA'G
Cuts at: 0 353 740 1074
Size: 353 387 334
Fragments arranged by size:
387 353 334
PvuII CAG'CTG
Cuts at: 0 507 996 1074
Size: 507 489 78
Fragments arranged by size:
507 489 78
RsrII CG'GwC_CG
Cuts at: 0 1057 1074
Size: 1057 17
SacI G_AGCT'C
Cuts at: 0 980 1074
Size: 980 94
SacII CC_GC'GG
Cuts at: 0 1020 1074
Size: 1020 54
SapI GCTCTTCn'nnn_
Cuts at: 0 925 1074
Size: 925 149
SfcI C'TryA_G
Cuts at: 0 349 600 736 1074
Size: 349 251 136 338
Fragments arranged by size:
349 338 251 136
SfoI GGC'GCC
Cuts at: 0 386 630 1074
Size: 386 244 444
Fragments arranged by size:
444 386 244
SmaI CCC'GGG
Cuts at: 0 892 1034 1074
Size: 892 142 40
Fragments arranged by size:
892 142 40
StuI AGG'CCT
Cuts at: 0 256 1074
Size: 256 818
StyI C'CwwG_G
Cuts at: 0 773 824 971 1074
Size: 773 51 147 103
Fragments arranged by size:
773 147 103 51
TatI w'GTAC_w
Cuts at: 0 290 1074
Size: 290 784
XmaI C'CCGG_G
Cuts at: 0 890 1032 1074
Size: 890 142 42
Fragments arranged by size:
890 142 42
Enzymes that do cut:
AccI AcuI AfeI AflIII AleI ApaI AvaI BanI
BanII BbeI BbsI BbvCI BcgI BglI BglII BlpI
Bme1580I BpmI Bpu10I BsaI BsaAI BsaHI BsaWI BseYI
BsgI BsiEI BsiHKAI Bsp1286I BspEI BspMI BsrBI BsrDI
BsrFI BssHII BstBI BstYI BtgI BtsI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I FspI HaeII
HincII KasI MslI MspA1I NaeI NarI NcoI NgoMIV
NotI NspI PasI PpiI PpuMI PspOMI PstI PvuII
RsrII SacI SacII SapI SfcI SfoI SmaI StuI
StyI TatI XmaI
Enzymes that do not cut:
AarI AatII Acc65I AclI AflII AgeI AhdI AlfI
AloI AlwNI ApaLI ApoI AscI AseI AsiSI AvrII
BaeI BamHI BciVI BclI BfrBI BmgBI BmrI BmtI
BplI BpuEI BsaBI BsaXI BseRI BsiWI BsmI BsmBI
BspHI BsrGI BssSI BstAPI BstEII BstXI BstZ17I Bsu36I
BtgZI ClaI CspCI DraI DraIII DrdI EcoRI EcoRV
FalI FseI FspAI Hin4I HindIII HpaI KpnI MfeI
MluI MmeI MscI NdeI NheI NruI NsiI PacI
PciI PflMI PfoI PmeI PmlI PshAI PsiI PspXI
PsrI PvuI SalI SanDI SbfI ScaI SexAI SfiI
SgfI SgrAI SmlI SnaBI SpeI SphI SrfI SspI
SwaI TaqII TstI Tth111I XbaI XcmI XhoI XmnI
ZraI