Restriction map for
pFN21AE9518
(Linear) (Six-base) MAPSORT of: KIEE9518.seq Check: 9779 from: 1 to: 966
KIEE9518.orf
With 182 enzymes: SgfI *
December 27, 2011 16:41 ..
Acc65I G'GTAC_C
Cuts at: 0 386 966
Size: 386 580
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 880 966
Size: 880 86
AfeI AGC'GCT
Cuts at: 0 358 966
Size: 358 608
AflIII A'CryG_T
Cuts at: 0 890 966
Size: 890 76
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 166 198 966
Size: 166 32 768
Fragments arranged by size:
768 166 32
AlwNI CAG_nnn'CTG
Cuts at: 0 858 966
Size: 858 108
ApaI G_GGCC'C
Cuts at: 0 444 966
Size: 444 522
BamHI G'GATC_C
Cuts at: 0 3 613 722 966
Size: 3 610 109 244
Fragments arranged by size:
610 244 109 3
BanI G'GyrC_C
Cuts at: 0 386 966
Size: 386 580
BanII G_rGCy'C
Cuts at: 0 444 829 882 966
Size: 444 385 53 84
Fragments arranged by size:
444 385 84 53
BbsI GAAGACnn'nnnn_
Cuts at: 0 663 966
Size: 663 303
BbvCI CC'TCA_GC
Cuts at: 0 226 966
Size: 226 740
BciVI GTATCCnnnnn_n'
Cuts at: 0 277 966
Size: 277 689
BclI T'GATC_A
Cuts at: 0 596 966
Size: 596 370
Bme1580I G_kGCm'C
Cuts at: 0 444 966
Size: 444 522
BmgBI CAC'GTC
Cuts at: 0 101 966
Size: 101 865
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 487 856 958 966
Size: 487 369 102 8
Fragments arranged by size:
487 369 102 8
Bpu10I CC'TnA_GC
Cuts at: 0 226 328 966
Size: 226 102 638
Fragments arranged by size:
638 226 102
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 102 966
Size: 102 864
BsaAI yAC'GTr
Cuts at: 0 645 729 966
Size: 645 84 237
Fragments arranged by size:
645 237 84
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 166 196 460 490 966
Size: 166 30 264 30 476
Fragments arranged by size:
476 264 166 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 70 667 966
Size: 70 597 299
Fragments arranged by size:
597 299 70
BsiEI CG_ry'CG
Cuts at: 0 234 966
Size: 234 732
BsiHKAI G_wGCw'C
Cuts at: 0 146 352 966
Size: 146 206 614
Fragments arranged by size:
614 206 146
BsmI GAATG_Cn'
Cuts at: 0 698 966
Size: 698 268
Bsp1286I G_dGCh'C
Cuts at: 0 146 352 444 829 882 966
Size: 146 206 92 385 53 84
Fragments arranged by size:
385 206 146 92 84 53
BsrBI CCG'CTC
Cuts at: 0 784 966
Size: 784 182
BstYI r'GATC_y
Cuts at: 0 3 613 722 966
Size: 3 610 109 244
Fragments arranged by size:
610 244 109 3
Bsu36I CC'TnA_GG
Cuts at: 0 926 966
Size: 926 40
BtsI GCAGTG_nn'
Cuts at: 0 801 966
Size: 801 165
DrdI GACnn_nn'nnGTC
Cuts at: 0 739 966
Size: 739 227
EaeI y'GGCC_r
Cuts at: 0 203 231 966
Size: 203 28 735
Fragments arranged by size:
735 203 28
EagI C'GGCC_G
Cuts at: 0 231 966
Size: 231 735
EarI CTCTTCn'nnn_
Cuts at: 0 226 966
Size: 226 740
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 487 856 880 958 966
Size: 487 369 24 78 8
Fragments arranged by size:
487 369 78 24 8
EcoO109I rG'GnC_Cy
Cuts at: 0 441 966
Size: 441 525
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 96 966
Size: 96 870
FspI TGC'GCA
Cuts at: 0 169 966
Size: 169 797
HaeII r_GCGC'y
Cuts at: 0 360 966
Size: 360 606
HindIII A'AGCT_T
Cuts at: 0 255 966
Size: 255 711
KpnI G_GTAC'C
Cuts at: 0 390 966
Size: 390 576
MluI A'CGCG_T
Cuts at: 0 890 966
Size: 890 76
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 31 966
Size: 31 935
MspA1I CmG'CkG
Cuts at: 0 230 392 418 630 966
Size: 230 162 26 212 336
Fragments arranged by size:
336 230 212 162 26
NotI GC'GGCC_GC
Cuts at: 0 231 966
Size: 231 735
NspI r_CATG'y
Cuts at: 0 168 966
Size: 168 798
PfoI T'CCnGG_A
Cuts at: 0 464 616 935 966
Size: 464 152 319 31
Fragments arranged by size:
464 319 152 31
PmlI CAC'GTG
Cuts at: 0 729 966
Size: 729 237
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 166 198 966
Size: 166 32 768
Fragments arranged by size:
768 166 32
PspOMI G'GGCC_C
Cuts at: 0 440 966
Size: 440 526
PstI C_TGCA'G
Cuts at: 0 887 966
Size: 887 79
ScaI AGT'ACT
Cuts at: 0 665 966
Size: 665 301
SfcI C'TryA_G
Cuts at: 0 883 966
Size: 883 83
SmlI C'TyrA_G
Cuts at: 0 81 966
Size: 81 885
SphI G_CATG'C
Cuts at: 0 168 966
Size: 168 798
StuI AGG'CCT
Cuts at: 0 326 966
Size: 326 640
StyI C'CwwG_G
Cuts at: 0 20 966
Size: 20 946
TatI w'GTAC_w
Cuts at: 0 663 966
Size: 663 303
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 465 497 594 626 966
Size: 465 32 97 32 340
Fragments arranged by size:
465 340 97 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 18 966
Size: 18 948
XmnI GAAnn'nnTTC
Cuts at: 0 178 966
Size: 178 788
Enzymes that do cut:
Acc65I AcuI AfeI AflIII AloI AlwNI ApaI BamHI
BanI BanII BbsI BbvCI BciVI BclI Bme1580I BmgBI
BpmI Bpu10I BpuEI BsaAI BsaXI BseRI BsiEI BsiHKAI
BsmI Bsp1286I BsrBI BstYI Bsu36I BtsI DrdI EaeI
EagI EarI Eco57MI EcoO109I FalI FspI HaeII HindIII
KpnI MluI MmeI MspA1I NotI NspI PfoI PmlI
PpiI PspOMI PstI ScaI SfcI SmlI SphI StuI
StyI TatI TstI XcmI XmnI
Enzymes that do not cut:
AarI AatII AccI AclI AflII AgeI AhdI AleI
AlfI ApaLI ApoI AscI AseI AsiSI AvaI AvrII
BaeI BbeI BcgI BfrBI BglI BglII BlpI BmrI
BmtI BplI BsaI BsaBI BsaHI BsaWI BseYI BsgI
BsiWI BsmBI BspEI BspHI BspMI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstZ17I BtgI
BtgZI ClaI CspCI DraI DraIII EciI EcoICRI EcoNI
EcoRI EcoRV FseI FspAI Hin4I HincII HpaI KasI
MfeI MscI MslI NaeI NarI NcoI NdeI NgoMIV
NheI NruI NsiI PacI PasI PciI PflMI PmeI
PpuMI PshAI PsiI PspXI PsrI PvuI PvuII RsrII
SacI SacII SalI SanDI SapI SbfI SexAI SfiI
SfoI SgfI SgrAI SmaI SnaBI SpeI SrfI SspI
SwaI TaqII Tth111I XbaI XhoI XmaI ZraI