Restriction map for
pFN21AE9517
(Linear) (Six-base) MAPSORT of: KIEE9517.seq Check: 8422 from: 1 to: 1722
KIEE9517.orf
With 182 enzymes: SgfI *
December 27, 2011 16:41 ..
AccI GT'mk_AC
Cuts at: 0 1205 1292 1722
Size: 1205 87 430
Fragments arranged by size:
1205 430 87
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 828 1404 1722
Size: 828 576 318
Fragments arranged by size:
828 576 318
AfeI AGC'GCT
Cuts at: 0 952 1722
Size: 952 770
AflIII A'CryG_T
Cuts at: 0 491 1722
Size: 491 1231
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 142 1675 1722
Size: 142 1533 47
Fragments arranged by size:
1533 142 47
ApaI G_GGCC'C
Cuts at: 0 514 1722
Size: 514 1208
ApaLI G'TGCA_C
Cuts at: 0 168 247 327 457 1722
Size: 168 79 80 130 1265
Fragments arranged by size:
1265 168 130 80 79
AvaI C'yCGr_G
Cuts at: 0 241 427 582 1722
Size: 241 186 155 1140
Fragments arranged by size:
1140 241 186 155
BanI G'GyrC_C
Cuts at: 0 85 645 691 988 1278 1372 1670 1722
Size: 85 560 46 297 290 94 298 52
Fragments arranged by size:
560 298 297 290 94 85 52 46
BanII G_rGCy'C
Cuts at: 0 514 1573 1722
Size: 514 1059 149
Fragments arranged by size:
1059 514 149
BbeI G_GCGC'C
Cuts at: 0 89 695 1282 1674 1722
Size: 89 606 587 392 48
Fragments arranged by size:
606 587 392 89 48
BbsI GAAGACnn'nnnn_
Cuts at: 0 1410 1578 1722
Size: 1410 168 144
Fragments arranged by size:
1410 168 144
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 159 193 318 352 1722
Size: 159 34 125 34 1370
Fragments arranged by size:
1370 159 125 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 1357 1722
Size: 1357 365
BclI T'GATC_A
Cuts at: 0 1592 1722
Size: 1592 130
BglI GCCn_nnn'nGGC
Cuts at: 0 432 518 1123 1156 1550 1722
Size: 432 86 605 33 394 172
Fragments arranged by size:
605 432 394 172 86 33
BglII A'GATC_T
Cuts at: 0 473 1722
Size: 473 1249
BlpI GC'TnA_GC
Cuts at: 0 1219 1722
Size: 1219 503
Bme1580I G_kGCm'C
Cuts at: 0 51 172 251 331 461 514 650 991
Size: 51 121 79 80 130 53 136 341
Cuts at: 991 1166 1442 1541 1722
Size: 175 276 99 181
Fragments arranged by size:
341 276 181 175 136 130 121 99
80 79 53 51
BmgBI CAC'GTC
Cuts at: 0 494 1310 1520 1722
Size: 494 816 210 202
Fragments arranged by size:
816 494 210 202
BmrI ACTGGGnnnn_n'
Cuts at: 0 1503 1722
Size: 1503 219
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 972 1146 1722
Size: 972 174 576
Fragments arranged by size:
972 576 174
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1230 1411 1722
Size: 1230 181 311
Fragments arranged by size:
1230 311 181
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 625 1722
Size: 625 1097
BsaAI yAC'GTr
Cuts at: 0 909 1722
Size: 909 813
BsaHI Gr'CG_yC
Cuts at: 0 86 606 692 1279 1415 1671 1722
Size: 86 520 86 587 136 256 51
Fragments arranged by size:
587 520 256 136 86 86 51
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 754 757 1722
Size: 754 3 965
Fragments arranged by size:
965 754 3
BseYI C'CCAG_C
Cuts at: 0 66 210 1722
Size: 66 144 1512
Fragments arranged by size:
1512 144 66
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 295 990 1722
Size: 295 695 732
Fragments arranged by size:
732 695 295
BsiEI CG_ry'CG
Cuts at: 0 20 1094 1722
Size: 20 1074 628
Fragments arranged by size:
1074 628 20
BsiHKAI G_wGCw'C
Cuts at: 0 172 251 331 338 461 1028 1430 1493
Size: 172 79 80 7 123 567 402 63
Cuts at: 1493 1722
Size: 229
Fragments arranged by size:
567 402 229 172 123 80 79 63
7
Bsp1286I G_dGCh'C
Cuts at: 0 51 172 251 331 338 461 514 650
Size: 51 121 79 80 7 123 53 136
Cuts at: 650 991 1028 1166 1430 1442 1493 1541 1573
Size: 341 37 138 264 12 51 48 32
Cuts at: 1573 1722
Size: 149
Fragments arranged by size:
341 264 149 138 136 123 121 80
79 53 51 51 48 37 32 12
7
BspHI T'CATG_A
Cuts at: 0 401 1358 1589 1722
Size: 401 957 231 133
Fragments arranged by size:
957 401 231 133
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 215 560 569 1223 1722
Size: 215 345 9 654 499
Fragments arranged by size:
654 499 345 215 9
BsrBI CCG'CTC
Cuts at: 0 22 432 858 1223 1722
Size: 22 410 426 365 499
Fragments arranged by size:
499 426 410 365 22
BsrFI r'CCGG_y
Cuts at: 0 913 1093 1324 1722
Size: 913 180 231 398
Fragments arranged by size:
913 398 231 180
BssHII G'CGCG_C
Cuts at: 0 375 1722
Size: 375 1347
BssSI C'ACGA_G
Cuts at: 0 1111 1722
Size: 1111 611
BstXI CCAn_nnnn'nTGG
Cuts at: 0 210 1455 1722
Size: 210 1245 267
Fragments arranged by size:
1245 267 210
BstYI r'GATC_y
Cuts at: 0 473 1640 1722
Size: 473 1167 82
Fragments arranged by size:
1167 473 82
BtgI C'CryG_G
Cuts at: 0 80 105 135 899 1193 1722
Size: 80 25 30 764 294 529
Fragments arranged by size:
764 529 294 80 30 25
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 162 1722
Size: 162 1560
BtsI GCAGTG_nn'
Cuts at: 0 283 527 1722
Size: 283 244 1195
Fragments arranged by size:
1195 283 244
DrdI GACnn_nn'nnGTC
Cuts at: 0 1582 1662 1722
Size: 1582 80 60
Fragments arranged by size:
1582 80 60
EaeI y'GGCC_r
Cuts at: 0 17 896 911 1043 1091 1148 1190 1322
Size: 17 879 15 132 48 57 42 132
Cuts at: 1322 1551 1722
Size: 229 171
Fragments arranged by size:
879 229 171 132 132 57 48 42
17 15
EagI C'GGCC_G
Cuts at: 0 17 1091 1722
Size: 17 1074 631
Fragments arranged by size:
1074 631 17
EarI CTCTTCn'nnn_
Cuts at: 0 1021 1303 1345 1722
Size: 1021 282 42 377
Fragments arranged by size:
1021 377 282 42
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 828 1230 1404 1411 1722
Size: 828 402 174 7 311
Fragments arranged by size:
828 402 311 174 7
EcoNI CCTnn'n_nnAGG
Cuts at: 0 925 1722
Size: 925 797
EcoO109I rG'GnC_Cy
Cuts at: 0 6 510 587 1212 1722
Size: 6 504 77 625 510
Fragments arranged by size:
625 510 504 77 6
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 432 1722
Size: 432 1290
FspI TGC'GCA
Cuts at: 0 1408 1722
Size: 1408 314
FspAI rTGC'GCAy
Cuts at: 0 1408 1722
Size: 1408 314
HaeII r_GCGC'y
Cuts at: 0 64 89 400 695 753 843 954 1282
Size: 64 25 311 295 58 90 111 328
Cuts at: 1282 1674 1722
Size: 392 48
Fragments arranged by size:
392 328 311 295 111 90 64 58
48 25
KasI G'GCGC_C
Cuts at: 0 85 691 1278 1670 1722
Size: 85 606 587 392 52
Fragments arranged by size:
606 587 392 85 52
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 476 1722
Size: 476 1246
MscI TGG'CCA
Cuts at: 0 1045 1192 1722
Size: 1045 147 530
Fragments arranged by size:
1045 530 147
MslI CAynn'nnrTG
Cuts at: 0 124 1357 1722
Size: 124 1233 365
Fragments arranged by size:
1233 365 124
MspA1I CmG'CkG
Cuts at: 0 82 165 264 1722
Size: 82 83 99 1458
Fragments arranged by size:
1458 99 83 82
NaeI GCC'GGC
Cuts at: 0 915 1095 1326 1722
Size: 915 180 231 396
Fragments arranged by size:
915 396 231 180
NarI GG'CG_CC
Cuts at: 0 86 692 1279 1671 1722
Size: 86 606 587 392 51
Fragments arranged by size:
606 587 392 86 51
NcoI C'CATG_G
Cuts at: 0 1193 1722
Size: 1193 529
NgoMIV G'CCGG_C
Cuts at: 0 913 1093 1324 1722
Size: 913 180 231 398
Fragments arranged by size:
913 398 231 180
NotI GC'GGCC_GC
Cuts at: 0 17 1722
Size: 17 1705
NspI r_CATG'y
Cuts at: 0 837 1722
Size: 837 885
PasI CC'CwG_GG
Cuts at: 0 36 1722
Size: 36 1686
PflMI CCAn_nnn'nTGG
Cuts at: 0 1058 1722
Size: 1058 664
PmlI CAC'GTG
Cuts at: 0 909 1722
Size: 909 813
PpuMI rG'GwC_Cy
Cuts at: 0 6 1212 1722
Size: 6 1206 510
Fragments arranged by size:
1206 510 6
PspOMI G'GGCC_C
Cuts at: 0 510 1722
Size: 510 1212
PspXI vC'TCGA_Gb
Cuts at: 0 241 1722
Size: 241 1481
PstI C_TGCA'G
Cuts at: 0 1517 1664 1722
Size: 1517 147 58
Fragments arranged by size:
1517 147 58
SacII CC_GC'GG
Cuts at: 0 83 1722
Size: 83 1639
SanDI GG'GwC_CC
Cuts at: 0 6 1722
Size: 6 1716
ScaI AGT'ACT
Cuts at: 0 1138 1722
Size: 1138 584
SexAI A'CCwGG_T
Cuts at: 0 824 1722
Size: 824 898
SfcI C'TryA_G
Cuts at: 0 1513 1660 1722
Size: 1513 147 62
Fragments arranged by size:
1513 147 62
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 1123 1156 1722
Size: 1123 33 566
Fragments arranged by size:
1123 566 33
SfoI GGC'GCC
Cuts at: 0 87 693 1280 1672 1722
Size: 87 606 587 392 50
Fragments arranged by size:
606 587 392 87 50
SmaI CCC'GGG
Cuts at: 0 584 1722
Size: 584 1138
SmlI C'TyrA_G
Cuts at: 0 241 640 1722
Size: 241 399 1082
Fragments arranged by size:
1082 399 241
StuI AGG'CCT
Cuts at: 0 1480 1722
Size: 1480 242
StyI C'CwwG_G
Cuts at: 0 1193 1722
Size: 1193 529
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 664 756 1396 1722
Size: 664 92 640 326
Fragments arranged by size:
664 640 326 92
TatI w'GTAC_w
Cuts at: 0 728 1136 1722
Size: 728 408 586
Fragments arranged by size:
728 586 408
XbaI T'CTAG_A
Cuts at: 0 924 1722
Size: 924 798
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1017 1185 1452 1722
Size: 1017 168 267 270
Fragments arranged by size:
1017 270 267 168
XhoI C'TCGA_G
Cuts at: 0 241 1722
Size: 241 1481
XmaI C'CCGG_G
Cuts at: 0 582 1722
Size: 582 1140
XmnI GAAnn'nnTTC
Cuts at: 0 409 1722
Size: 409 1313
Enzymes that do cut:
AccI AcuI AfeI AflIII AlfI ApaI ApaLI AvaI
BanI BanII BbeI BbsI BcgI BciVI BclI BglI
BglII BlpI Bme1580I BmgBI BmrI BplI BpmI BpuEI
BsaAI BsaHI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I
BspHI BspMI BsrBI BsrFI BssHII BssSI BstXI BstYI
BtgI BtgZI BtsI DrdI EaeI EagI EarI Eco57MI
EcoNI EcoO109I FalI FspI FspAI HaeII KasI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NotI
NspI PasI PflMI PmlI PpuMI PspOMI PspXI PstI
SacII SanDI ScaI SexAI SfcI SfiI SfoI SmaI
SmlI StuI StyI TaqII TatI XbaI XcmI XhoI
XmaI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AflII AgeI AhdI AleI
AloI AlwNI ApoI AscI AseI AsiSI AvrII BaeI
BamHI BbvCI BfrBI BmtI Bpu10I BsaI BsaBI BsaWI
BsaXI BsiWI BsmI BsmBI BspEI BsrDI BsrGI BstAPI
BstBI BstEII BstZ17I Bsu36I ClaI CspCI DraI DraIII
EciI EcoICRI EcoRI EcoRV FseI Hin4I HincII HindIII
HpaI KpnI MfeI MluI NdeI NheI NruI NsiI
PacI PciI PfoI PmeI PpiI PshAI PsiI PsrI
PvuI PvuII RsrII SacI SalI SapI SbfI SgfI
SgrAI SnaBI SpeI SphI SrfI SspI SwaI TstI
Tth111I ZraI