Restriction map for
pFN21AE9513
(Linear) (Six-base) MAPSORT of: KIEE9513.seq Check: 8387 from: 1 to: 1095
KIEE9513.orf
With 182 enzymes: SgfI *
December 27, 2011 16:41 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 388 1028 1095
Size: 388 640 67
Fragments arranged by size:
640 388 67
AatII G_ACGT'C
Cuts at: 0 945 1095
Size: 945 150
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 582 593 1095
Size: 582 11 502
Fragments arranged by size:
582 502 11
AflIII A'CryG_T
Cuts at: 0 218 1095
Size: 218 877
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 406 1095
Size: 406 689
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 85 117 1095
Size: 85 32 978
Fragments arranged by size:
978 85 32
AlwNI CAG_nnn'CTG
Cuts at: 0 52 700 1067 1095
Size: 52 648 367 28
Fragments arranged by size:
648 367 52 28
ApaLI G'TGCA_C
Cuts at: 0 391 592 1095
Size: 391 201 503
Fragments arranged by size:
503 391 201
AvrII C'CTAG_G
Cuts at: 0 33 1078 1095
Size: 33 1045 17
Fragments arranged by size:
1045 33 17
BamHI G'GATC_C
Cuts at: 0 903 1095
Size: 903 192
BbsI GAAGACnn'nnnn_
Cuts at: 0 704 1095
Size: 704 391
BbvCI CC'TCA_GC
Cuts at: 0 40 1095
Size: 40 1055
BglI GCCn_nnn'nGGC
Cuts at: 0 455 1095
Size: 455 640
BglII A'GATC_T
Cuts at: 0 317 1095
Size: 317 778
Bme1580I G_kGCm'C
Cuts at: 0 395 429 506 596 635 1095
Size: 395 34 77 90 39 460
Fragments arranged by size:
460 395 90 77 39 34
BmrI ACTGGGnnnn_n'
Cuts at: 0 935 1095
Size: 935 160
Bpu10I CC'TnA_GC
Cuts at: 0 40 1095
Size: 40 1055
BsaHI Gr'CG_yC
Cuts at: 0 942 1095
Size: 942 153
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 932 962 1095
Size: 932 30 133
Fragments arranged by size:
932 133 30
BseYI C'CCAG_C
Cuts at: 0 141 434 977 1095
Size: 141 293 543 118
Fragments arranged by size:
543 293 141 118
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 686 1095
Size: 686 409
BsiHKAI G_wGCw'C
Cuts at: 0 395 596 914 1095
Size: 395 201 318 181
Fragments arranged by size:
395 318 201 181
Bsp1286I G_dGCh'C
Cuts at: 0 395 429 506 596 635 914 1095
Size: 395 34 77 90 39 279 181
Fragments arranged by size:
395 279 181 90 77 39 34
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 388 1028 1095
Size: 388 640 67
Fragments arranged by size:
640 388 67
BsrBI CCG'CTC
Cuts at: 0 725 1095
Size: 725 370
BsrFI r'CCGG_y
Cuts at: 0 490 1095
Size: 490 605
BssSI C'ACGA_G
Cuts at: 0 499 1095
Size: 499 596
BstXI CCAn_nnnn'nTGG
Cuts at: 0 170 744 819 1095
Size: 170 574 75 276
Fragments arranged by size:
574 276 170 75
BstYI r'GATC_y
Cuts at: 0 28 317 903 1095
Size: 28 289 586 192
Fragments arranged by size:
586 289 192 28
Bsu36I CC'TnA_GG
Cuts at: 0 1022 1095
Size: 1022 73
BtsI GCAGTG_nn'
Cuts at: 0 66 369 1095
Size: 66 303 726
Fragments arranged by size:
726 303 66
DraIII CAC_nnn'GTG
Cuts at: 0 591 1095
Size: 591 504
EaeI y'GGCC_r
Cuts at: 0 2 209 809 881 1046 1095
Size: 2 207 600 72 165 49
Fragments arranged by size:
600 207 165 72 49 2
EarI CTCTTCn'nnn_
Cuts at: 0 181 569 1002 1095
Size: 181 388 433 93
Fragments arranged by size:
433 388 181 93
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 175 1095
Size: 175 920
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 582 593 1095
Size: 582 11 502
Fragments arranged by size:
582 502 11
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1021 1095
Size: 1021 74
EcoO109I rG'GnC_Cy
Cuts at: 0 49 1095
Size: 49 1046
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 932 964 1095
Size: 932 32 131
Fragments arranged by size:
932 131 32
HincII GTy'rAC
Cuts at: 0 852 1095
Size: 852 243
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 216 800 1095
Size: 216 584 295
Fragments arranged by size:
584 295 216
MscI TGG'CCA
Cuts at: 0 4 811 883 1095
Size: 4 807 72 212
Fragments arranged by size:
807 212 72 4
MslI CAynn'nnrTG
Cuts at: 0 217 388 742 1095
Size: 217 171 354 353
Fragments arranged by size:
354 353 217 171
MspA1I CmG'CkG
Cuts at: 0 896 1034 1095
Size: 896 138 61
Fragments arranged by size:
896 138 61
NaeI GCC'GGC
Cuts at: 0 492 1095
Size: 492 603
NgoMIV G'CCGG_C
Cuts at: 0 490 1095
Size: 490 605
NspI r_CATG'y
Cuts at: 0 222 1095
Size: 222 873
PasI CC'CwG_GG
Cuts at: 0 197 1095
Size: 197 898
PciI A'CATG_T
Cuts at: 0 218 1095
Size: 218 877
PflMI CCAn_nnn'nTGG
Cuts at: 0 52 1095
Size: 52 1043
PpuMI rG'GwC_Cy
Cuts at: 0 49 1095
Size: 49 1046
PshAI GACnn'nnGTC
Cuts at: 0 544 1095
Size: 544 551
PstI C_TGCA'G
Cuts at: 0 364 1039 1095
Size: 364 675 56
Fragments arranged by size:
675 364 56
PvuII CAG'CTG
Cuts at: 0 896 1034 1095
Size: 896 138 61
Fragments arranged by size:
896 138 61
SapI GCTCTTCn'nnn_
Cuts at: 0 181 1095
Size: 181 914
ScaI AGT'ACT
Cuts at: 0 839 1095
Size: 839 256
SfcI C'TryA_G
Cuts at: 0 360 1035 1095
Size: 360 675 60
Fragments arranged by size:
675 360 60
SpeI A'CTAG_T
Cuts at: 0 1008 1095
Size: 1008 87
StuI AGG'CCT
Cuts at: 0 38 458 1095
Size: 38 420 637
Fragments arranged by size:
637 420 38
StyI C'CwwG_G
Cuts at: 0 33 1078 1095
Size: 33 1045 17
Fragments arranged by size:
1045 33 17
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 431 1095
Size: 431 664
TatI w'GTAC_w
Cuts at: 0 7 837 1095
Size: 7 830 258
Fragments arranged by size:
830 258 7
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 304 804 897 1095
Size: 304 500 93 198
Fragments arranged by size:
500 304 198 93
ZraI GAC'GTC
Cuts at: 0 943 1095
Size: 943 152
Enzymes that do cut:
AarI AatII AcuI AflIII AlfI AloI AlwNI ApaLI
AvrII BamHI BbsI BbvCI BglI BglII Bme1580I BmrI
Bpu10I BsaHI BsaXI BseYI BsgI BsiHKAI Bsp1286I BspMI
BsrBI BsrFI BssSI BstXI BstYI Bsu36I BtsI DraIII
EaeI EarI EciI Eco57MI EcoNI EcoO109I Hin4I HincII
MmeI MscI MslI MspA1I NaeI NgoMIV NspI PasI
PciI PflMI PpuMI PshAI PstI PvuII SapI ScaI
SfcI SpeI StuI StyI TaqII TatI XcmI ZraI
Enzymes that do not cut:
AccI Acc65I AclI AfeI AflII AgeI AhdI AleI
ApaI ApoI AscI AseI AsiSI AvaI BaeI BanI
BanII BbeI BcgI BciVI BclI BfrBI BlpI BmgBI
BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaWI
BseRI BsiEI BsiWI BsmI BsmBI BspEI BspHI BsrDI
BsrGI BssHII BstAPI BstBI BstEII BstZ17I BtgI BtgZI
ClaI CspCI DraI DrdI EagI EcoICRI EcoRI EcoRV
FalI FseI FspI FspAI HaeII HindIII HpaI KasI
KpnI MfeI MluI NarI NcoI NdeI NheI NotI
NruI NsiI PacI PfoI PmeI PmlI PpiI PsiI
PspOMI PspXI PsrI PvuI RsrII SacI SacII SalI
SanDI SbfI SexAI SfiI SfoI SgfI SgrAI SmaI
SmlI SnaBI SphI SrfI SspI SwaI TstI Tth111I
XbaI XhoI XmaI XmnI