Restriction map for
pFN21AE9512
(Linear) (Six-base) MAPSORT of: KIEE9512.seq Check: 1014 from: 1 to: 1596
KIEE9512.orf
With 182 enzymes: SgfI *
December 27, 2011 16:41 ..
AccI GT'mk_AC
Cuts at: 0 971 1596
Size: 971 625
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1037 1488 1596
Size: 1037 451 108
Fragments arranged by size:
1037 451 108
AleI CACnn'nnGTG
Cuts at: 0 133 1596
Size: 133 1463
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 516 1596
Size: 516 1080
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 790 822 1596
Size: 790 32 774
Fragments arranged by size:
790 774 32
AlwNI CAG_nnn'CTG
Cuts at: 0 755 1099 1329 1596
Size: 755 344 230 267
Fragments arranged by size:
755 344 267 230
ApaI G_GGCC'C
Cuts at: 0 489 1596
Size: 489 1107
ApoI r'AATT_y
Cuts at: 0 1006 1596
Size: 1006 590
AvaI C'yCGr_G
Cuts at: 0 292 1596
Size: 292 1304
BanI G'GyrC_C
Cuts at: 0 40 1596
Size: 40 1556
BanII G_rGCy'C
Cuts at: 0 291 489 572 744 1596
Size: 291 198 83 172 852
Fragments arranged by size:
852 291 198 172 83
BbsI GAAGACnn'nnnn_
Cuts at: 0 1516 1596
Size: 1516 80
BclI T'GATC_A
Cuts at: 0 125 368 1596
Size: 125 243 1228
Fragments arranged by size:
1228 243 125
BglI GCCn_nnn'nGGC
Cuts at: 0 880 1596
Size: 880 716
BglII A'GATC_T
Cuts at: 0 225 1596
Size: 225 1371
BlpI GC'TnA_GC
Cuts at: 0 208 213 890 1596
Size: 208 5 677 706
Fragments arranged by size:
706 677 208 5
Bme1580I G_kGCm'C
Cuts at: 0 489 1596
Size: 489 1107
BmrI ACTGGGnnnn_n'
Cuts at: 0 40 851 1216 1411 1596
Size: 40 811 365 195 185
Fragments arranged by size:
811 365 195 185 40
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 673 1076 1596
Size: 673 403 520
Fragments arranged by size:
673 520 403
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 166 1596
Size: 166 1430
BsaI GGTCTCn'nnnn_
Cuts at: 0 1168 1596
Size: 1168 428
BsaAI yAC'GTr
Cuts at: 0 333 1596
Size: 333 1263
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 790 820 1596
Size: 790 30 776
Fragments arranged by size:
790 776 30
BseYI C'CCAG_C
Cuts at: 0 284 489 785 1264 1596
Size: 284 205 296 479 332
Fragments arranged by size:
479 332 296 284 205
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 242 1596
Size: 242 1354
BsiHKAI G_wGCw'C
Cuts at: 0 832 1596
Size: 832 764
Bsp1286I G_dGCh'C
Cuts at: 0 291 489 572 744 832 1596
Size: 291 198 83 172 88 764
Fragments arranged by size:
764 291 198 172 88 83
BspHI T'CATG_A
Cuts at: 0 152 353 446 623 1596
Size: 152 201 93 177 973
Fragments arranged by size:
973 201 177 152 93
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 212 1596
Size: 212 1384
BsrDI GCAATG_nn'
Cuts at: 0 95 147 593 1596
Size: 95 52 446 1003
Fragments arranged by size:
1003 446 95 52
BstXI CCAn_nnnn'nTGG
Cuts at: 0 678 862 1344 1596
Size: 678 184 482 252
Fragments arranged by size:
678 482 252 184
BstYI r'GATC_y
Cuts at: 0 225 551 751 1596
Size: 225 326 200 845
Fragments arranged by size:
845 326 225 200
BtsI GCAGTG_nn'
Cuts at: 0 587 1596
Size: 587 1009
DraIII CAC_nnn'GTG
Cuts at: 0 1197 1333 1427 1596
Size: 1197 136 94 169
Fragments arranged by size:
1197 169 136 94
DrdI GACnn_nn'nnGTC
Cuts at: 0 1115 1411 1596
Size: 1115 296 185
Fragments arranged by size:
1115 296 185
EaeI y'GGCC_r
Cuts at: 0 335 1226 1596
Size: 335 891 370
Fragments arranged by size:
891 370 335
EarI CTCTTCn'nnn_
Cuts at: 0 715 1013 1553 1596
Size: 715 298 540 43
Fragments arranged by size:
715 540 298 43
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 673 1037 1076 1488 1596
Size: 673 364 39 412 108
Fragments arranged by size:
673 412 364 108 39
EcoNI CCTnn'n_nnAGG
Cuts at: 0 822 1596
Size: 822 774
EcoO109I rG'GnC_Cy
Cuts at: 0 486 675 1596
Size: 486 189 921
Fragments arranged by size:
921 486 189
EcoRI G'AATT_C
Cuts at: 0 1006 1596
Size: 1006 590
HaeII r_GCGC'y
Cuts at: 0 875 1596
Size: 875 721
HincII GTy'rAC
Cuts at: 0 171 192 1470 1596
Size: 171 21 1278 126
Fragments arranged by size:
1278 171 126 21
HpaI GTT'AAC
Cuts at: 0 192 1596
Size: 192 1404
MfeI C'AATT_G
Cuts at: 0 876 1596
Size: 876 720
MscI TGG'CCA
Cuts at: 0 337 1228 1596
Size: 337 891 368
Fragments arranged by size:
891 368 337
MslI CAynn'nnrTG
Cuts at: 0 133 274 1596
Size: 133 141 1322
Fragments arranged by size:
1322 141 133
MspA1I CmG'CkG
Cuts at: 0 103 789 1099 1596
Size: 103 686 310 497
Fragments arranged by size:
686 497 310 103
PasI CC'CwG_GG
Cuts at: 0 997 1596
Size: 997 599
PflMI CCAn_nnn'nTGG
Cuts at: 0 63 1596
Size: 63 1533
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 790 822 1596
Size: 790 32 774
Fragments arranged by size:
790 774 32
PpuMI rG'GwC_Cy
Cuts at: 0 675 1596
Size: 675 921
PspOMI G'GGCC_C
Cuts at: 0 485 1596
Size: 485 1111
PvuII CAG'CTG
Cuts at: 0 103 1099 1596
Size: 103 996 497
Fragments arranged by size:
996 497 103
ScaI AGT'ACT
Cuts at: 0 511 1596
Size: 511 1085
SfcI C'TryA_G
Cuts at: 0 906 1596
Size: 906 690
SmlI C'TyrA_G
Cuts at: 0 181 1596
Size: 181 1415
StuI AGG'CCT
Cuts at: 0 805 1250 1596
Size: 805 445 346
Fragments arranged by size:
805 445 346
StyI C'CwwG_G
Cuts at: 0 671 1229 1596
Size: 671 558 367
Fragments arranged by size:
671 558 367
TatI w'GTAC_w
Cuts at: 0 509 1568 1596
Size: 509 1059 28
Fragments arranged by size:
1059 509 28
Tth111I GACn'n_nGTC
Cuts at: 0 955 1596
Size: 955 641
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 875 1424 1596
Size: 875 549 172
Fragments arranged by size:
875 549 172
Enzymes that do cut:
AccI AcuI AleI AlfI AloI AlwNI ApaI ApoI
AvaI BanI BanII BbsI BclI BglI BglII BlpI
Bme1580I BmrI BpmI BpuEI BsaI BsaAI BsaXI BseYI
BsgI BsiHKAI Bsp1286I BspHI BspMI BsrDI BstXI BstYI
BtsI DraIII DrdI EaeI EarI Eco57MI EcoNI EcoO109I
EcoRI HaeII HincII HpaI MfeI MscI MslI MspA1I
PasI PflMI PpiI PpuMI PspOMI PvuII ScaI SfcI
SmlI StuI StyI TatI Tth111I XcmI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AflIII AgeI
AhdI ApaLI AscI AseI AsiSI AvrII BaeI BamHI
BbeI BbvCI BcgI BciVI BfrBI BmgBI BmtI BplI
Bpu10I BsaBI BsaHI BsaWI BseRI BsiEI BsiWI BsmI
BsmBI BspEI BsrBI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstZ17I Bsu36I BtgI BtgZI ClaI CspCI
DraI EagI EciI EcoICRI EcoRV FalI FseI FspI
FspAI Hin4I HindIII KasI KpnI MluI MmeI NaeI
NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI
NspI PacI PciI PfoI PmeI PmlI PshAI PsiI
PspXI PsrI PstI PvuI RsrII SacI SacII SalI
SanDI SapI SbfI SexAI SfiI SfoI SgfI SgrAI
SmaI SnaBI SpeI SphI SrfI SspI SwaI TaqII
TstI XbaI XhoI XmaI XmnI ZraI