Restriction map for
pFN21AE9511
(Linear) (Six-base) MAPSORT of: KIEE9511.seq Check: 821 from: 1 to: 984
KIEE9511.orf
With 182 enzymes: SgfI *
December 27, 2011 16:41 ..
AccI GT'mk_AC
Cuts at: 0 79 128 984
Size: 79 49 856
Fragments arranged by size:
856 79 49
AflIII A'CryG_T
Cuts at: 0 74 179 984
Size: 74 105 805
Fragments arranged by size:
805 105 74
AleI CACnn'nnGTG
Cuts at: 0 262 622 814 984
Size: 262 360 192 170
Fragments arranged by size:
360 262 192 170
ApaI G_GGCC'C
Cuts at: 0 49 984
Size: 49 935
AvaI C'yCGr_G
Cuts at: 0 583 984
Size: 583 401
BanI G'GyrC_C
Cuts at: 0 87 99 192 554 984
Size: 87 12 93 362 430
Fragments arranged by size:
430 362 93 87 12
BanII G_rGCy'C
Cuts at: 0 26 49 254 320 984
Size: 26 23 205 66 664
Fragments arranged by size:
664 205 66 26 23
BbeI G_GCGC'C
Cuts at: 0 91 103 196 558 984
Size: 91 12 93 362 426
Fragments arranged by size:
426 362 93 91 12
BbsI GAAGACnn'nnnn_
Cuts at: 0 211 516 984
Size: 211 305 468
Fragments arranged by size:
468 305 211
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 315 349 984
Size: 315 34 635
Fragments arranged by size:
635 315 34
BlpI GC'TnA_GC
Cuts at: 0 61 984
Size: 61 923
Bme1580I G_kGCm'C
Cuts at: 0 49 984
Size: 49 935
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 348 745 984
Size: 348 397 239
Fragments arranged by size:
397 348 239
Bpu10I CC'TnA_GC
Cuts at: 0 805 883 984
Size: 805 78 101
Fragments arranged by size:
805 101 78
BsaAI yAC'GTr
Cuts at: 0 180 984
Size: 180 804
BsaHI Gr'CG_yC
Cuts at: 0 88 100 193 555 793 844 932 984
Size: 88 12 93 362 238 51 88 52
Fragments arranged by size:
362 238 93 88 88 52 51 12
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 307 337 346 376 984
Size: 307 30 9 30 608
Fragments arranged by size:
608 307 30 30 9
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 772 984
Size: 772 212
BseYI C'CCAG_C
Cuts at: 0 627 984
Size: 627 357
BsiEI CG_ry'CG
Cuts at: 0 399 447 984
Size: 399 48 537
Fragments arranged by size:
537 399 48
BsiHKAI G_wGCw'C
Cuts at: 0 254 320 811 984
Size: 254 66 491 173
Fragments arranged by size:
491 254 173 66
Bsp1286I G_dGCh'C
Cuts at: 0 26 49 254 320 811 984
Size: 26 23 205 66 491 173
Fragments arranged by size:
491 205 173 66 26 23
BspHI T'CATG_A
Cuts at: 0 386 984
Size: 386 598
BsrFI r'CCGG_y
Cuts at: 0 96 442 551 683 984
Size: 96 346 109 132 301
Fragments arranged by size:
346 301 132 109 96
BssHII G'CGCG_C
Cuts at: 0 463 465 600 729 984
Size: 463 2 135 129 255
Fragments arranged by size:
463 255 135 129 2
BssSI C'ACGA_G
Cuts at: 0 622 984
Size: 622 362
BstAPI GCAn_nnn'nTGC
Cuts at: 0 696 984
Size: 696 288
BstEII G'GTnAC_C
Cuts at: 0 744 984
Size: 744 240
BstXI CCAn_nnnn'nTGG
Cuts at: 0 702 723 984
Size: 702 21 261
Fragments arranged by size:
702 261 21
BtgI C'CryG_G
Cuts at: 0 146 812 984
Size: 146 666 172
Fragments arranged by size:
666 172 146
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 225 264 840 984
Size: 225 39 576 144
Fragments arranged by size:
576 225 144 39
BtsI GCAGTG_nn'
Cuts at: 0 307 419 984
Size: 307 112 565
Fragments arranged by size:
565 307 112
EaeI y'GGCC_r
Cuts at: 0 236 416 440 444 479 984
Size: 236 180 24 4 35 505
Fragments arranged by size:
505 236 180 35 24 4
EagI C'GGCC_G
Cuts at: 0 444 984
Size: 444 540
EarI CTCTTCn'nnn_
Cuts at: 0 259 984
Size: 259 725
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 156 943 984
Size: 156 787 41
Fragments arranged by size:
787 156 41
EcoICRI GAG'CTC
Cuts at: 0 252 318 984
Size: 252 66 666
Fragments arranged by size:
666 252 66
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 348 745 984
Size: 348 397 239
Fragments arranged by size:
397 348 239
EcoO109I rG'GnC_Cy
Cuts at: 0 720 984
Size: 720 264
FseI GG_CCGG'CC
Cuts at: 0 446 984
Size: 446 538
HaeII r_GCGC'y
Cuts at: 0 61 91 103 196 395 461 558 823
Size: 61 30 12 93 199 66 97 265
Cuts at: 823 984
Size: 161
Fragments arranged by size:
265 199 161 97 93 66 61 30
12
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 27 984
Size: 27 957
HincII GTy'rAC
Cuts at: 0 80 984
Size: 80 904
KasI G'GCGC_C
Cuts at: 0 87 99 192 554 984
Size: 87 12 93 362 430
Fragments arranged by size:
430 362 93 87 12
MluI A'CGCG_T
Cuts at: 0 74 984
Size: 74 910
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 95 984
Size: 95 889
MscI TGG'CCA
Cuts at: 0 238 418 984
Size: 238 180 566
Fragments arranged by size:
566 238 180
MslI CAynn'nnrTG
Cuts at: 0 262 385 622 643 814 984
Size: 262 123 237 21 171 170
Fragments arranged by size:
262 237 171 170 123 21
MspA1I CmG'CkG
Cuts at: 0 93 105 849 899 960 984
Size: 93 12 744 50 61 24
Fragments arranged by size:
744 93 61 50 24 12
NaeI GCC'GGC
Cuts at: 0 98 444 553 685 984
Size: 98 346 109 132 299
Fragments arranged by size:
346 299 132 109 98
NarI GG'CG_CC
Cuts at: 0 88 100 193 555 984
Size: 88 12 93 362 429
Fragments arranged by size:
429 362 93 88 12
NgoMIV G'CCGG_C
Cuts at: 0 96 442 551 683 984
Size: 96 346 109 132 301
Fragments arranged by size:
346 301 132 109 96
NspI r_CATG'y
Cuts at: 0 694 984
Size: 694 290
PfoI T'CCnGG_A
Cuts at: 0 926 984
Size: 926 58
PspOMI G'GGCC_C
Cuts at: 0 45 984
Size: 45 939
PvuII CAG'CTG
Cuts at: 0 899 960 984
Size: 899 61 24
Fragments arranged by size:
899 61 24
SacI G_AGCT'C
Cuts at: 0 254 320 984
Size: 254 66 664
Fragments arranged by size:
664 254 66
SalI G'TCGA_C
Cuts at: 0 78 984
Size: 78 906
SapI GCTCTTCn'nnn_
Cuts at: 0 259 984
Size: 259 725
SexAI A'CCwGG_T
Cuts at: 0 404 984
Size: 404 580
SfcI C'TryA_G
Cuts at: 0 453 984
Size: 453 531
SfoI GGC'GCC
Cuts at: 0 89 101 194 556 984
Size: 89 12 93 362 428
Fragments arranged by size:
428 362 93 89 12
SphI G_CATG'C
Cuts at: 0 694 984
Size: 694 290
StuI AGG'CCT
Cuts at: 0 589 984
Size: 589 395
StyI C'CwwG_G
Cuts at: 0 716 984
Size: 716 268
TatI w'GTAC_w
Cuts at: 0 350 984
Size: 350 634
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 307 339 984
Size: 307 32 645
Fragments arranged by size:
645 307 32
Enzymes that do cut:
AccI AflIII AleI ApaI AvaI BanI BanII BbeI
BbsI BcgI BlpI Bme1580I BpmI Bpu10I BsaAI BsaHI
BsaXI BseRI BseYI BsiEI BsiHKAI Bsp1286I BspHI BsrFI
BssHII BssSI BstAPI BstEII BstXI BtgI BtgZI BtsI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I FseI
HaeII Hin4I HincII KasI MluI MmeI MscI MslI
MspA1I NaeI NarI NgoMIV NspI PfoI PspOMI PvuII
SacI SalI SapI SexAI SfcI SfoI SphI StuI
StyI TatI TstI
Enzymes that do not cut:
AarI AatII Acc65I AclI AcuI AfeI AflII AgeI
AhdI AlfI AloI AlwNI ApaLI ApoI AscI AseI
AsiSI AvrII BaeI BamHI BbvCI BciVI BclI BfrBI
BglI BglII BmgBI BmrI BmtI BplI BpuEI BsaI
BsaBI BsaWI BsgI BsiWI BsmI BsmBI BspEI BspMI
BsrBI BsrDI BsrGI BstBI BstYI BstZ17I Bsu36I ClaI
CspCI DraI DraIII DrdI EcoNI EcoRI EcoRV FalI
FspI FspAI HindIII HpaI KpnI MfeI NcoI NdeI
NheI NotI NruI NsiI PacI PasI PciI PflMI
PmeI PmlI PpiI PpuMI PshAI PsiI PspXI PsrI
PstI PvuI RsrII SacII SanDI SbfI ScaI SfiI
SgfI SgrAI SmaI SmlI SnaBI SpeI SrfI SspI
SwaI TaqII Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI