Restriction map for
pFN21AE9510
(Linear) (Six-base) MAPSORT of: KIEE9510.seq Check: 2021 from: 1 to: 1437
KIEE9510.orf
With 182 enzymes: SgfI *
December 27, 2011 16:41 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1406 1437
Size: 1406 31
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 198 1437
Size: 198 1239
AfeI AGC'GCT
Cuts at: 0 836 1437
Size: 836 601
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1168 1437
Size: 1168 269
AlwNI CAG_nnn'CTG
Cuts at: 0 567 1437
Size: 567 870
ApaI G_GGCC'C
Cuts at: 0 700 996 1437
Size: 700 296 441
Fragments arranged by size:
700 441 296
AvaI C'yCGr_G
Cuts at: 0 30 691 775 788 1178 1437
Size: 30 661 84 13 390 259
Fragments arranged by size:
661 390 259 84 30 13
BanI G'GyrC_C
Cuts at: 0 244 301 492 537 1394 1437
Size: 244 57 191 45 857 43
Fragments arranged by size:
857 244 191 57 45 43
BanII G_rGCy'C
Cuts at: 0 700 742 996 1437
Size: 700 42 254 441
Fragments arranged by size:
700 441 254 42
BbeI G_GCGC'C
Cuts at: 0 248 305 496 1437
Size: 248 57 191 941
Fragments arranged by size:
941 248 191 57
BbsI GAAGACnn'nnnn_
Cuts at: 0 90 1437
Size: 90 1347
BglI GCCn_nnn'nGGC
Cuts at: 0 1090 1117 1437
Size: 1090 27 320
Fragments arranged by size:
1090 320 27
Bme1580I G_kGCm'C
Cuts at: 0 542 700 996 1437
Size: 542 158 296 441
Fragments arranged by size:
542 441 296 158
BmgBI CAC'GTC
Cuts at: 0 59 1437
Size: 59 1378
BmtI G_CTAG'C
Cuts at: 0 1222 1437
Size: 1222 215
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 906 1437
Size: 906 531
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 835 993 1437
Size: 835 158 444
Fragments arranged by size:
835 444 158
Bpu10I CC'TnA_GC
Cuts at: 0 124 891 1437
Size: 124 767 546
Fragments arranged by size:
767 546 124
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 718 1437
Size: 718 719
BsaAI yAC'GTr
Cuts at: 0 339 1437
Size: 339 1098
BsaHI Gr'CG_yC
Cuts at: 0 245 302 493 1240 1437
Size: 245 57 191 747 197
Fragments arranged by size:
747 245 197 191 57
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 991 1021 1437
Size: 991 30 416
Fragments arranged by size:
991 416 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 808 832 1437
Size: 808 24 605
Fragments arranged by size:
808 605 24
BseYI C'CCAG_C
Cuts at: 0 576 924 1002 1437
Size: 576 348 78 435
Fragments arranged by size:
576 435 348 78
BsiHKAI G_wGCw'C
Cuts at: 0 488 1437
Size: 488 949
BsmBI CGTCTCn'nnnn_
Cuts at: 0 76 1437
Size: 76 1361
Bsp1286I G_dGCh'C
Cuts at: 0 488 542 700 742 996 1437
Size: 488 54 158 42 254 441
Fragments arranged by size:
488 441 254 158 54 42
BspHI T'CATG_A
Cuts at: 0 359 452 629 1437
Size: 359 93 177 808
Fragments arranged by size:
808 359 177 93
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 623 1406 1437
Size: 623 783 31
Fragments arranged by size:
783 623 31
BsrDI GCAATG_nn'
Cuts at: 0 101 599 810 1056 1437
Size: 101 498 211 246 381
Fragments arranged by size:
498 381 246 211 101
BsrFI r'CCGG_y
Cuts at: 0 686 1089 1437
Size: 686 403 348
Fragments arranged by size:
686 403 348
BsrGI T'GTAC_A
Cuts at: 0 272 641 1437
Size: 272 369 796
Fragments arranged by size:
796 369 272
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1401 1437
Size: 1401 36
BstXI CCAn_nnnn'nTGG
Cuts at: 0 441 723 1437
Size: 441 282 714
Fragments arranged by size:
714 441 282
BstYI r'GATC_y
Cuts at: 0 1022 1437
Size: 1022 415
BtgI C'CryG_G
Cuts at: 0 305 857 988 1437
Size: 305 552 131 449
Fragments arranged by size:
552 449 305 131
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1122 1437
Size: 1122 315
BtsI GCAGTG_nn'
Cuts at: 0 587 903 1427 1437
Size: 587 316 524 10
Fragments arranged by size:
587 524 316 10
DraI TTT'AAA
Cuts at: 0 1380 1437
Size: 1380 57
DraIII CAC_nnn'GTG
Cuts at: 0 1301 1437
Size: 1301 136
EaeI y'GGCC_r
Cuts at: 0 2 659 1109 1437
Size: 2 657 450 328
Fragments arranged by size:
657 450 328 2
EarI CTCTTCn'nnn_
Cuts at: 0 217 1437
Size: 217 1220
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 198 835 993 1437
Size: 198 637 158 444
Fragments arranged by size:
637 444 198 158
EcoO109I rG'GnC_Cy
Cuts at: 0 696 822 993 1437
Size: 696 126 171 444
Fragments arranged by size:
696 444 171 126
FspI TGC'GCA
Cuts at: 0 802 1156 1437
Size: 802 354 281
Fragments arranged by size:
802 354 281
HaeII r_GCGC'y
Cuts at: 0 248 305 496 838 1437
Size: 248 57 191 342 599
Fragments arranged by size:
599 342 248 191 57
HincII GTy'rAC
Cuts at: 0 177 198 1332 1437
Size: 177 21 1134 105
Fragments arranged by size:
1134 177 105 21
KasI G'GCGC_C
Cuts at: 0 244 301 492 1437
Size: 244 57 191 945
Fragments arranged by size:
945 244 191 57
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 596 1437
Size: 596 841
MscI TGG'CCA
Cuts at: 0 4 661 1437
Size: 4 657 776
Fragments arranged by size:
776 657 4
MslI CAynn'nnrTG
Cuts at: 0 154 439 634 1437
Size: 154 285 195 803
Fragments arranged by size:
803 285 195 154
MspA1I CmG'CkG
Cuts at: 0 186 990 1437
Size: 186 804 447
Fragments arranged by size:
804 447 186
NaeI GCC'GGC
Cuts at: 0 1091 1437
Size: 1091 346
NarI GG'CG_CC
Cuts at: 0 245 302 493 1437
Size: 245 57 191 944
Fragments arranged by size:
944 245 191 57
NgoMIV G'CCGG_C
Cuts at: 0 1089 1437
Size: 1089 348
NheI G'CTAG_C
Cuts at: 0 1218 1437
Size: 1218 219
NspI r_CATG'y
Cuts at: 0 1101 1437
Size: 1101 336
PasI CC'CwG_GG
Cuts at: 0 297 1437
Size: 297 1140
PspOMI G'GGCC_C
Cuts at: 0 696 992 1437
Size: 696 296 445
Fragments arranged by size:
696 445 296
PstI C_TGCA'G
Cuts at: 0 191 986 1437
Size: 191 795 451
Fragments arranged by size:
795 451 191
PvuII CAG'CTG
Cuts at: 0 186 1437
Size: 186 1251
SacII CC_GC'GG
Cuts at: 0 991 1437
Size: 991 446
SexAI A'CCwGG_T
Cuts at: 0 1148 1437
Size: 1148 289
SfcI C'TryA_G
Cuts at: 0 187 982 1437
Size: 187 795 455
Fragments arranged by size:
795 455 187
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 1117 1437
Size: 1117 320
SfoI GGC'GCC
Cuts at: 0 246 303 494 1437
Size: 246 57 191 943
Fragments arranged by size:
943 246 191 57
SmaI CCC'GGG
Cuts at: 0 777 790 1180 1437
Size: 777 13 390 257
Fragments arranged by size:
777 390 257 13
SmlI C'TyrA_G
Cuts at: 0 733 1437
Size: 733 704
SphI G_CATG'C
Cuts at: 0 1101 1437
Size: 1101 336
StuI AGG'CCT
Cuts at: 0 449 1437
Size: 449 988
StyI C'CwwG_G
Cuts at: 0 914 1437
Size: 914 523
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 919 1437
Size: 919 518
TatI w'GTAC_w
Cuts at: 0 272 641 1437
Size: 272 369 796
Fragments arranged by size:
796 369 272
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1010 1437
Size: 1010 427
XmaI C'CCGG_G
Cuts at: 0 775 788 1178 1437
Size: 775 13 390 259
Fragments arranged by size:
775 390 259 13
XmnI GAAnn'nnTTC
Cuts at: 0 1270 1437
Size: 1270 167
Enzymes that do cut:
AarI AcuI AfeI AlfI AlwNI ApaI AvaI BanI
BanII BbeI BbsI BglI Bme1580I BmgBI BmtI BplI
BpmI Bpu10I BpuEI BsaAI BsaHI BsaXI BseRI BseYI
BsiHKAI BsmBI Bsp1286I BspHI BspMI BsrDI BsrFI BsrGI
BstAPI BstXI BstYI BtgI BtgZI BtsI DraI DraIII
EaeI EarI Eco57MI EcoO109I FspI HaeII HincII KasI
MmeI MscI MslI MspA1I NaeI NarI NgoMIV NheI
NspI PasI PspOMI PstI PvuII SacII SexAI SfcI
SfiI SfoI SmaI SmlI SphI StuI StyI TaqII
TatI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AflII AflIII AgeI AhdI
AleI AloI ApaLI ApoI AscI AseI AsiSI AvrII
BaeI BamHI BbvCI BcgI BciVI BclI BfrBI BglII
BlpI BmrI BsaI BsaBI BsaWI BsgI BsiEI BsiWI
BsmI BspEI BsrBI BssHII BssSI BstBI BstEII BstZ17I
Bsu36I ClaI CspCI DrdI EagI EciI EcoICRI EcoNI
EcoRI EcoRV FalI FseI FspAI Hin4I HindIII HpaI
KpnI MfeI MluI NcoI NdeI NotI NruI NsiI
PacI PciI PflMI PfoI PmeI PmlI PpiI PpuMI
PshAI PsiI PspXI PsrI PvuI RsrII SacI SalI
SanDI SapI SbfI ScaI SgfI SgrAI SnaBI SpeI
SrfI SspI SwaI TstI Tth111I XbaI XhoI ZraI