(Linear) (Six-base) MAPSORT of: KIEE9506.seq  Check: 2356  from: 1  to: 996



KIEE9506.orf

 With 182 enzymes: SgfI * 

                        December 27, 2011 16:40  ..

AflIII A'CryG_T

Cuts at:      0     641     996 
   Size:        641     355
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0      92     426     996 
   Size:         92     334     570

  Fragments arranged by size: 

                 570     334      92
 
ApaI G_GGCC'C

Cuts at:      0     777     996 
   Size:        777     219
 
BanI G'GyrC_C

Cuts at:      0     697     712     996 
   Size:        697      15     284

  Fragments arranged by size: 

                 697     284      15
 
BanII G_rGCy'C

Cuts at:      0     777     996 
   Size:        777     219
 
BclI T'GATC_A

Cuts at:      0     869     996 
   Size:        869     127
 
Bme1580I G_kGCm'C

Cuts at:      0     777     996 
   Size:        777     219
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     448     996 
   Size:        448     548
 
BsaHI Gr'CG_yC

Cuts at:      0     755     996 
   Size:        755     241
 
BsaWI w'CCGG_w

Cuts at:      0     911     996 
   Size:        911      85
 
BseYI C'CCAG_C

Cuts at:      0     770     996 
   Size:        770     226
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      22      52     996 
   Size:         22      30     944

  Fragments arranged by size: 

                 944      30      22
 
BsmI GAATG_Cn'

Cuts at:      0     225     996 
   Size:        225     771
 
Bsp1286I G_dGCh'C

Cuts at:      0     777     996 
   Size:        777     219
 
BspEI T'CCGG_A

Cuts at:      0     911     996 
   Size:        911      85
 
BsrDI GCAATG_nn'

Cuts at:      0      81     996 
   Size:         81     915
 
Bsu36I CC'TnA_GG

Cuts at:      0     143     996 
   Size:        143     853
 
BtgI C'CryG_G

Cuts at:      0      18     996 
   Size:         18     978
 
BtsI GCAGTG_nn'

Cuts at:      0     437     996 
   Size:        437     559
 
ClaI AT'CG_AT

Cuts at:      0     890     996 
   Size:        890     106
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     413     448     996 
   Size:        413      35     548

  Fragments arranged by size: 

                 548     413      35
 
EaeI y'GGCC_r

Cuts at:      0     653     996 
   Size:        653     343
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     694     996 
   Size:        694     302
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     448     996 
   Size:        448     548
 
EcoO109I rG'GnC_Cy

Cuts at:      0     774     996 
   Size:        774     222
 
MfeI C'AATT_G

Cuts at:      0     425     996 
   Size:        425     571
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     158     996 
   Size:        158     838
 
MscI TGG'CCA

Cuts at:      0     655     996 
   Size:        655     341
 
MslI CAynn'nnrTG

Cuts at:      0      74     595     996 
   Size:         74     521     401

  Fragments arranged by size: 

                 521     401      74
 
NspI r_CATG'y

Cuts at:      0     645     996 
   Size:        645     351
 
PasI CC'CwG_GG

Cuts at:      0     963     996 
   Size:        963      33
 
PciI A'CATG_T

Cuts at:      0     641     996 
   Size:        641     355
 
PspOMI G'GGCC_C

Cuts at:      0     773     996 
   Size:        773     223
 
PstI C_TGCA'G

Cuts at:      0     253     996 
   Size:        253     743
 
SfcI C'TryA_G

Cuts at:      0     249     387     996 
   Size:        249     138     609

  Fragments arranged by size: 

                 609     249     138
 
SspI AAT'ATT

Cuts at:      0     360     797     996 
   Size:        360     437     199

  Fragments arranged by size: 

                 437     360     199
 
StuI AGG'CCT

Cuts at:      0     971     996 
   Size:        971      25
 
StyI C'CwwG_G

Cuts at:      0      90     708     996 
   Size:         90     618     288

  Fragments arranged by size: 

                 618     288      90
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0      28     151     246     996 
   Size:         28     123      95     750

  Fragments arranged by size: 

                 750     123      95      28
 
TatI w'GTAC_w

Cuts at:      0     236     325     996 
   Size:        236      89     671

  Fragments arranged by size: 

                 671     236      89
 
XmnI GAAnn'nnTTC

Cuts at:      0     934     996 
   Size:        934      62
 

 Enzymes that do cut:

   AflIII     AlfI     ApaI     BanI    BanII     BclI Bme1580I     BpmI
    BsaHI    BsaWI    BseYI     BsgI     BsmI Bsp1286I    BspEI    BsrDI
   Bsu36I     BtgI     BtsI     ClaI    CspCI     EaeI     EciI  Eco57MI
 EcoO109I     MfeI     MmeI     MscI     MslI     NspI     PasI     PciI
   PspOMI     PstI     SfcI     SspI     StuI     StyI    TaqII     TatI
     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
     AgeI     AhdI     AleI     AloI    AlwNI    ApaLI     ApoI     AscI
     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BbeI     BbsI
    BbvCI     BcgI    BciVI    BfrBI     BglI    BglII     BlpI    BmgBI
     BmrI     BmtI     BplI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI
    BsaXI    BseRI    BsiEI  BsiHKAI    BsiWI    BsmBI    BspHI    BspMI
    BsrBI    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII
    BstXI    BstYI  BstZ17I    BtgZI     DraI   DraIII     DrdI     EagI
     EarI  EcoICRI    EcoNI    EcoRI    EcoRV     FalI     FseI     FspI
    FspAI    HaeII    Hin4I   HincII  HindIII     HpaI     KasI     KpnI
     MluI   MspA1I     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI
     NotI     NruI     NsiI     PacI    PflMI     PfoI     PmeI     PmlI
     PpiI    PpuMI    PshAI     PsiI    PspXI     PsrI     PvuI    PvuII
    RsrII     SacI    SacII     SalI    SanDI     SapI     SbfI     ScaI
    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI
     SpeI     SphI     SrfI     SwaI     TstI  Tth111I     XbaI     XcmI
     XhoI     XmaI     ZraI