(Linear) (Six-base) MAPSORT of: KIEE9463.seq  Check: 9585  from: 1  to: 1002



KIEE9463.orf

 With 182 enzymes: SgfI * 

                        December 27, 2011 16:40  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     175     888    1002 
   Size:        175     713     114

  Fragments arranged by size: 

                 713     175     114
 
AlwNI CAG_nnn'CTG

Cuts at:      0      55    1002 
   Size:         55     947
 
ApaLI G'TGCA_C

Cuts at:      0     219     801    1002 
   Size:        219     582     201

  Fragments arranged by size: 

                 582     219     201
 
BanI G'GyrC_C

Cuts at:      0     505    1002 
   Size:        505     497
 
BanII G_rGCy'C

Cuts at:      0     972    1002 
   Size:        972      30
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     870    1002 
   Size:        870     132
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     792     826    1002 
   Size:        792      34     176

  Fragments arranged by size: 

                 792     176      34
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     386    1002 
   Size:        386     616
 
Bme1580I G_kGCm'C

Cuts at:      0     223     805    1002 
   Size:        223     582     197

  Fragments arranged by size: 

                 582     223     197
 
BmgBI CAC'GTC

Cuts at:      0     276     747    1002 
   Size:        276     471     255

  Fragments arranged by size: 

                 471     276     255
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     295    1002 
   Size:        295     707
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     554    1002 
   Size:        554     448
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     530    1002 
   Size:        530     472
 
BseYI C'CCAG_C

Cuts at:      0       3    1002 
   Size:          3     999
 
BsiHKAI G_wGCw'C

Cuts at:      0     223     281     805    1002 
   Size:        223      58     524     197

  Fragments arranged by size: 

                 524     223     197      58
 
BsmI GAATG_Cn'

Cuts at:      0      27     326    1002 
   Size:         27     299     676

  Fragments arranged by size: 

                 676     299      27
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     266    1002 
   Size:        266     736
 
Bsp1286I G_dGCh'C

Cuts at:      0     223     281     805     972    1002 
   Size:        223      58     524     167      30

  Fragments arranged by size: 

                 524     223     167      58      30
 
BspHI T'CATG_A

Cuts at:      0     972    1002 
   Size:        972      30
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     648    1002 
   Size:        648     354
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0      22      55    1002 
   Size:         22      33     947

  Fragments arranged by size: 

                 947      33      22
 
BtsI GCAGTG_nn'

Cuts at:      0     716    1002 
   Size:        716     286
 
ClaI AT'CG_AT

Cuts at:      0     357    1002 
   Size:        357     645
 
EaeI y'GGCC_r

Cuts at:      0     431    1002 
   Size:        431     571
 
EarI CTCTTCn'nnn_

Cuts at:      0     640    1002 
   Size:        640     362
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     175     554     888    1002 
   Size:        175     379     334     114

  Fragments arranged by size: 

                 379     334     175     114
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     766    1002 
   Size:        766     236
 
MscI TGG'CCA

Cuts at:      0     433    1002 
   Size:        433     569
 
MslI CAynn'nnrTG

Cuts at:      0     709     916    1002 
   Size:        709     207      86

  Fragments arranged by size: 

                 709     207      86
 
NspI r_CATG'y

Cuts at:      0     567     915    1002 
   Size:        567     348      87

  Fragments arranged by size: 

                 567     348      87
 
PsiI TTA'TAA

Cuts at:      0     481     493    1002 
   Size:        481      12     509

  Fragments arranged by size: 

                 509     481      12
 
ScaI AGT'ACT

Cuts at:      0      70    1002 
   Size:         70     932
 
SspI AAT'ATT

Cuts at:      0     177    1002 
   Size:        177     825
 
StyI C'CwwG_G

Cuts at:      0     114     428    1002 
   Size:        114     314     574

  Fragments arranged by size: 

                 574     314     114
 
TatI w'GTAC_w

Cuts at:      0      68    1002 
   Size:         68     934
 
XmnI GAAnn'nnTTC

Cuts at:      0     392    1002 
   Size:        392     610
 

 Enzymes that do cut:

     AcuI    AlwNI    ApaLI     BanI    BanII     BbsI     BcgI    BciVI
 Bme1580I    BmgBI     BplI     BpmI     BsaI    BseYI  BsiHKAI     BsmI
    BsmBI Bsp1286I    BspHI    BspMI   BstAPI     BtsI     ClaI     EaeI
     EarI  Eco57MI     MmeI     MscI     MslI     NspI     PsiI     ScaI
     SspI     StyI     TatI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI     ApaI     ApoI     AscI
     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BbeI    BbvCI
     BclI    BfrBI     BglI    BglII     BlpI     BmrI     BmtI   Bpu10I
    BpuEI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI     BsgI
    BsiEI    BsiWI    BspEI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII
    BssSI    BstBI   BstEII    BstXI    BstYI  BstZ17I   Bsu36I     BtgI
    BtgZI    CspCI     DraI   DraIII     DrdI     EagI     EciI  EcoICRI
    EcoNI EcoO109I    EcoRI    EcoRV     FalI     FseI     FspI    FspAI
    HaeII    Hin4I   HincII  HindIII     HpaI     KasI     KpnI     MfeI
     MluI   MspA1I     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI
     NotI     NruI     NsiI     PacI     PasI     PciI    PflMI     PfoI
     PmeI     PmlI     PpiI    PpuMI    PshAI   PspOMI    PspXI     PsrI
     PstI     PvuI    PvuII    RsrII     SacI    SacII     SalI    SanDI
     SapI     SbfI    SexAI     SfcI     SfiI     SfoI     SgfI    SgrAI
     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI     StuI     SwaI
    TaqII     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI     ZraI