Restriction map for
pFN21AE9462
(Linear) (Six-base) MAPSORT of: KIEE9462.seq Check: 2313 from: 1 to: 1458
KIEE9462.orf
With 182 enzymes: SgfI *
December 27, 2011 16:40 ..
AccI GT'mk_AC
Cuts at: 0 922 1458
Size: 922 536
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1385 1458
Size: 1385 73
AfeI AGC'GCT
Cuts at: 0 844 1458
Size: 844 614
AflIII A'CryG_T
Cuts at: 0 1079 1370 1448 1458
Size: 1079 291 78 10
Fragments arranged by size:
1079 291 78 10
AleI CACnn'nnGTG
Cuts at: 0 328 1458
Size: 328 1130
AlwNI CAG_nnn'CTG
Cuts at: 0 118 1230 1458
Size: 118 1112 228
Fragments arranged by size:
1112 228 118
ApaI G_GGCC'C
Cuts at: 0 204 587 1458
Size: 204 383 871
Fragments arranged by size:
871 383 204
AvaI C'yCGr_G
Cuts at: 0 587 1027 1091 1458
Size: 587 440 64 367
Fragments arranged by size:
587 440 367 64
BanI G'GyrC_C
Cuts at: 0 799 1458
Size: 799 659
BanII G_rGCy'C
Cuts at: 0 204 587 1458
Size: 204 383 871
Fragments arranged by size:
871 383 204
BbsI GAAGACnn'nnnn_
Cuts at: 0 1373 1458
Size: 1373 85
BclI T'GATC_A
Cuts at: 0 659 1458
Size: 659 799
BglI GCCn_nnn'nGGC
Cuts at: 0 44 136 701 1458
Size: 44 92 565 757
Fragments arranged by size:
757 565 92 44
BlpI GC'TnA_GC
Cuts at: 0 92 1458
Size: 92 1366
Bme1580I G_kGCm'C
Cuts at: 0 204 587 1458
Size: 204 383 871
Fragments arranged by size:
871 383 204
BmgBI CAC'GTC
Cuts at: 0 546 1458
Size: 546 912
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 119 313 1458
Size: 119 194 1145
Fragments arranged by size:
1145 194 119
BsaI GGTCTCn'nnnn_
Cuts at: 0 1038 1102 1458
Size: 1038 64 356
Fragments arranged by size:
1038 356 64
BsaAI yAC'GTr
Cuts at: 0 1449 1458
Size: 1449 9
BsaBI GATnn'nnATC
Cuts at: 0 139 1458
Size: 139 1319
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 862 1458
Size: 862 596
BseYI C'CCAG_C
Cuts at: 0 304 393 593 719 795 1458
Size: 304 89 200 126 76 663
Fragments arranged by size:
663 304 200 126 89 76
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 388 1458
Size: 388 1070
BsiHKAI G_wGCw'C
Cuts at: 0 335 872 1458
Size: 335 537 586
Fragments arranged by size:
586 537 335
Bsp1286I G_dGCh'C
Cuts at: 0 204 335 587 872 1458
Size: 204 131 252 285 586
Fragments arranged by size:
586 285 252 204 131
BsrBI CCG'CTC
Cuts at: 0 269 1458
Size: 269 1189
BsrFI r'CCGG_y
Cuts at: 0 38 1176 1194 1458
Size: 38 1138 18 264
Fragments arranged by size:
1138 264 38 18
BssSI C'ACGA_G
Cuts at: 0 442 1458
Size: 442 1016
BstEII G'GTnAC_C
Cuts at: 0 1041 1458
Size: 1041 417
BstXI CCAn_nnnn'nTGG
Cuts at: 0 197 345 903 1257 1458
Size: 197 148 558 354 201
Fragments arranged by size:
558 354 201 197 148
BstZ17I GTA'TAC
Cuts at: 0 923 1458
Size: 923 535
Bsu36I CC'TnA_GG
Cuts at: 0 698 1458
Size: 698 760
BtgI C'CryG_G
Cuts at: 0 299 579 725 1166 1309 1458
Size: 299 280 146 441 143 149
Fragments arranged by size:
441 299 280 149 146 143
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 729 963 1458
Size: 729 234 495
Fragments arranged by size:
729 495 234
BtsI GCAGTG_nn'
Cuts at: 0 687 1347 1458
Size: 687 660 111
Fragments arranged by size:
687 660 111
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 873 908 1458
Size: 873 35 550
Fragments arranged by size:
873 550 35
EaeI y'GGCC_r
Cuts at: 0 137 350 831 974 1397 1458
Size: 137 213 481 143 423 61
Fragments arranged by size:
481 423 213 143 137 61
EarI CTCTTCn'nnn_
Cuts at: 0 184 439 1018 1458
Size: 184 255 579 440
Fragments arranged by size:
579 440 255 184
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 119 313 1385 1458
Size: 119 194 1072 73
Fragments arranged by size:
1072 194 119 73
EcoNI CCTnn'n_nnAGG
Cuts at: 0 631 1458
Size: 631 827
EcoO109I rG'GnC_Cy
Cuts at: 0 104 194 398 422 584 812 1458
Size: 104 90 204 24 162 228 646
Fragments arranged by size:
646 228 204 162 104 90 24
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 486 1458
Size: 486 972
HaeII r_GCGC'y
Cuts at: 0 495 846 1458
Size: 495 351 612
Fragments arranged by size:
612 495 351
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 161 193 1360 1392 1458
Size: 161 32 1167 32 66
Fragments arranged by size:
1167 161 66 32 32
HincII GTy'rAC
Cuts at: 0 19 1458
Size: 19 1439
MscI TGG'CCA
Cuts at: 0 139 352 976 1458
Size: 139 213 624 482
Fragments arranged by size:
624 482 213 139
MslI CAynn'nnrTG
Cuts at: 0 328 346 901 1144 1255 1458
Size: 328 18 555 243 111 203
Fragments arranged by size:
555 328 243 203 111 18
MspA1I CmG'CkG
Cuts at: 0 1211 1227 1458
Size: 1211 16 231
Fragments arranged by size:
1211 231 16
NaeI GCC'GGC
Cuts at: 0 40 1458
Size: 40 1418
NcoI C'CATG_G
Cuts at: 0 1166 1458
Size: 1166 292
NdeI CA'TA_TG
Cuts at: 0 898 1458
Size: 898 560
NgoMIV G'CCGG_C
Cuts at: 0 38 1458
Size: 38 1420
NspI r_CATG'y
Cuts at: 0 1083 1143 1374 1458
Size: 1083 60 231 84
Fragments arranged by size:
1083 231 84 60
PasI CC'CwG_GG
Cuts at: 0 190 204 426 1458
Size: 190 14 222 1032
Fragments arranged by size:
1032 222 190 14
PciI A'CATG_T
Cuts at: 0 1079 1370 1458
Size: 1079 291 88
Fragments arranged by size:
1079 291 88
PflMI CCAn_nnn'nTGG
Cuts at: 0 118 998 1335 1458
Size: 118 880 337 123
Fragments arranged by size:
880 337 123 118
PfoI T'CCnGG_A
Cuts at: 0 290 1458
Size: 290 1168
PpuMI rG'GwC_Cy
Cuts at: 0 104 398 422 1458
Size: 104 294 24 1036
Fragments arranged by size:
1036 294 104 24
PspOMI G'GGCC_C
Cuts at: 0 200 583 1458
Size: 200 383 875
Fragments arranged by size:
875 383 200
PstI C_TGCA'G
Cuts at: 0 229 1458
Size: 229 1229
PvuII CAG'CTG
Cuts at: 0 1227 1458
Size: 1227 231
SanDI GG'GwC_CC
Cuts at: 0 104 398 422 1458
Size: 104 294 24 1036
Fragments arranged by size:
1036 294 104 24
SfcI C'TryA_G
Cuts at: 0 225 1458
Size: 225 1233
SmaI CCC'GGG
Cuts at: 0 589 1093 1458
Size: 589 504 365
Fragments arranged by size:
589 504 365
StuI AGG'CCT
Cuts at: 0 236 1159 1458
Size: 236 923 299
Fragments arranged by size:
923 299 236
StyI C'CwwG_G
Cuts at: 0 152 231 1166 1458
Size: 152 79 935 292
Fragments arranged by size:
935 292 152 79
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 585 787 891 1458
Size: 585 202 104 567
Fragments arranged by size:
585 567 202 104
TatI w'GTAC_w
Cuts at: 0 248 1373 1458
Size: 248 1125 85
Fragments arranged by size:
1125 248 85
Tth111I GACn'n_nGTC
Cuts at: 0 760 1047 1458
Size: 760 287 411
Fragments arranged by size:
760 411 287
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 115 345 750 1095 1458
Size: 115 230 405 345 363
Fragments arranged by size:
405 363 345 230 115
XmaI C'CCGG_G
Cuts at: 0 587 1091 1458
Size: 587 504 367
Fragments arranged by size:
587 504 367
Enzymes that do cut:
AccI AcuI AfeI AflIII AleI AlwNI ApaI AvaI
BanI BanII BbsI BclI BglI BlpI Bme1580I BmgBI
BpmI BsaI BsaAI BsaBI BseRI BseYI BsgI BsiHKAI
Bsp1286I BsrBI BsrFI BssSI BstEII BstXI BstZ17I Bsu36I
BtgI BtgZI BtsI CspCI EaeI EarI Eco57MI EcoNI
EcoO109I FalI HaeII Hin4I HincII MscI MslI MspA1I
NaeI NcoI NdeI NgoMIV NspI PasI PciI PflMI
PfoI PpuMI PspOMI PstI PvuII SanDI SfcI SmaI
StuI StyI TaqII TatI Tth111I XcmI XmaI
Enzymes that do not cut:
AarI AatII Acc65I AclI AflII AgeI AhdI AlfI
AloI ApaLI ApoI AscI AseI AsiSI AvrII BaeI
BamHI BbeI BbvCI BcgI BciVI BfrBI BglII BmrI
BmtI BplI Bpu10I BpuEI BsaHI BsaWI BsaXI BsiEI
BsiWI BsmI BsmBI BspEI BspHI BspMI BsrDI BsrGI
BssHII BstAPI BstBI BstYI ClaI DraI DraIII DrdI
EagI EciI EcoICRI EcoRI EcoRV FseI FspI FspAI
HindIII HpaI KasI KpnI MfeI MluI MmeI NarI
NheI NotI NruI NsiI PacI PmeI PmlI PpiI
PshAI PsiI PspXI PsrI PvuI RsrII SacI SacII
SalI SapI SbfI ScaI SexAI SfiI SfoI SgfI
SgrAI SmlI SnaBI SpeI SphI SrfI SspI SwaI
TstI XbaI XhoI XmnI ZraI