Restriction map for
pFN21AE9460
(Linear) (Six-base) MAPSORT of: KIEE9460.seq Check: 5897 from: 1 to: 957
KIEE9460.orf
With 182 enzymes: SgfI *
December 27, 2011 16:40 ..
AccI GT'mk_AC
Cuts at: 0 736 957
Size: 736 221
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 265 325 957
Size: 265 60 632
Fragments arranged by size:
632 265 60
AleI CACnn'nnGTG
Cuts at: 0 140 957
Size: 140 817
ApoI r'AATT_y
Cuts at: 0 511 540 567 957
Size: 511 29 27 390
Fragments arranged by size:
511 390 29 27
AseI AT'TA_AT
Cuts at: 0 161 957
Size: 161 796
BanI G'GyrC_C
Cuts at: 0 231 438 957
Size: 231 207 519
Fragments arranged by size:
519 231 207
BbsI GAAGACnn'nnnn_
Cuts at: 0 331 957
Size: 331 626
BbvCI CC'TCA_GC
Cuts at: 0 742 957
Size: 742 215
BglI GCCn_nnn'nGGC
Cuts at: 0 802 957
Size: 802 155
BglII A'GATC_T
Cuts at: 0 36 362 957
Size: 36 326 595
Fragments arranged by size:
595 326 36
BmrI ACTGGGnnnn_n'
Cuts at: 0 19 957
Size: 19 938
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 61 957
Size: 61 896
Bpu10I CC'TnA_GC
Cuts at: 0 742 957
Size: 742 215
BsaI GGTCTCn'nnnn_
Cuts at: 0 907 957
Size: 907 50
BsaAI yAC'GTr
Cuts at: 0 53 957
Size: 53 904
BsaBI GATnn'nnATC
Cuts at: 0 457 957
Size: 457 500
BsmI GAATG_Cn'
Cuts at: 0 871 957
Size: 871 86
BsrBI CCG'CTC
Cuts at: 0 878 957
Size: 878 79
BstYI r'GATC_y
Cuts at: 0 36 362 957
Size: 36 326 595
Fragments arranged by size:
595 326 36
BstZ17I GTA'TAC
Cuts at: 0 737 957
Size: 737 220
BtgI C'CryG_G
Cuts at: 0 686 957
Size: 686 271
BtsI GCAGTG_nn'
Cuts at: 0 255 808 957
Size: 255 553 149
Fragments arranged by size:
553 255 149
DraI TTT'AAA
Cuts at: 0 504 566 957
Size: 504 62 391
Fragments arranged by size:
504 391 62
EarI CTCTTCn'nnn_
Cuts at: 0 793 957
Size: 793 164
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 61 265 325 957
Size: 61 204 60 632
Fragments arranged by size:
632 204 61 60
EcoRV GAT'ATC
Cuts at: 0 401 957
Size: 401 556
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 906 938 957
Size: 906 32 19
Fragments arranged by size:
906 32 19
HincII GTy'rAC
Cuts at: 0 219 957
Size: 219 738
MfeI C'AATT_G
Cuts at: 0 594 957
Size: 594 363
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 60 957
Size: 60 897
MslI CAynn'nnrTG
Cuts at: 0 140 957
Size: 140 817
MspA1I CmG'CkG
Cuts at: 0 335 345 356 957
Size: 335 10 11 601
Fragments arranged by size:
601 335 11 10
NdeI CA'TA_TG
Cuts at: 0 547 700 957
Size: 547 153 257
Fragments arranged by size:
547 257 153
PacI TTA_AT'TAA
Cuts at: 0 161 957
Size: 161 796
PshAI GACnn'nnGTC
Cuts at: 0 335 754 957
Size: 335 419 203
Fragments arranged by size:
419 335 203
PsiI TTA'TAA
Cuts at: 0 32 433 957
Size: 32 401 524
Fragments arranged by size:
524 401 32
PvuII CAG'CTG
Cuts at: 0 345 356 957
Size: 345 11 601
Fragments arranged by size:
601 345 11
SfcI C'TryA_G
Cuts at: 0 618 957
Size: 618 339
SspI AAT'ATT
Cuts at: 0 560 957
Size: 560 397
StyI C'CwwG_G
Cuts at: 0 235 917 957
Size: 235 682 40
Fragments arranged by size:
682 235 40
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 214 957
Size: 214 743
TatI w'GTAC_w
Cuts at: 0 851 957
Size: 851 106
XmnI GAAnn'nnTTC
Cuts at: 0 250 957
Size: 250 707
Enzymes that do cut:
AccI AcuI AleI ApoI AseI BanI BbsI BbvCI
BglI BglII BmrI BpmI Bpu10I BsaI BsaAI BsaBI
BsmI BsrBI BstYI BstZ17I BtgI BtsI DraI EarI
Eco57MI EcoRV Hin4I HincII MfeI MmeI MslI MspA1I
NdeI PacI PshAI PsiI PvuII SfcI SspI StyI
TaqII TatI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AflIII AgeI
AhdI AlfI AloI AlwNI ApaI ApaLI AscI AsiSI
AvaI AvrII BaeI BamHI BanII BbeI BcgI BciVI
BclI BfrBI BlpI Bme1580I BmgBI BmtI BplI BpuEI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsiWI BsmBI Bsp1286I BspEI BspHI BspMI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI Bsu36I
BtgZI ClaI CspCI DraIII DrdI EaeI EagI EciI
EcoICRI EcoNI EcoO109I EcoRI FalI FseI FspI FspAI
HaeII HindIII HpaI KasI KpnI MluI MscI NaeI
NarI NcoI NgoMIV NheI NotI NruI NsiI NspI
PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI
PspOMI PspXI PsrI PstI PvuI RsrII SacI SacII
SalI SanDI SapI SbfI ScaI SexAI SfiI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI
StuI SwaI TstI Tth111I XbaI XcmI XhoI XmaI
ZraI