Restriction map for
pFN21AE9458
(Linear) (Six-base) MAPSORT of: KIEE9458.seq Check: 3047 from: 1 to: 2193
KIEE9458.orf
With 182 enzymes: SgfI *
March 22, 2012 18:29 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 875 1544 2193
Size: 875 669 649
Fragments arranged by size:
875 669 649
AfeI AGC'GCT
Cuts at: 0 928 947 2193
Size: 928 19 1246
Fragments arranged by size:
1246 928 19
AflIII A'CryG_T
Cuts at: 0 118 2193
Size: 118 2075
AhdI GACnn_n'nnGTC
Cuts at: 0 1899 2193
Size: 1899 294
AleI CACnn'nnGTG
Cuts at: 0 407 1828 1840 2193
Size: 407 1421 12 353
Fragments arranged by size:
1421 407 353 12
ApaLI G'TGCA_C
Cuts at: 0 312 2193
Size: 312 1881
ApoI r'AATT_y
Cuts at: 0 692 1192 1266 1868 2193
Size: 692 500 74 602 325
Fragments arranged by size:
692 602 500 325 74
AseI AT'TA_AT
Cuts at: 0 2054 2193
Size: 2054 139
AvaI C'yCGr_G
Cuts at: 0 774 2193
Size: 774 1419
AvrII C'CTAG_G
Cuts at: 0 1995 2193
Size: 1995 198
BanI G'GyrC_C
Cuts at: 0 517 892 2193
Size: 517 375 1301
Fragments arranged by size:
1301 517 375
BanII G_rGCy'C
Cuts at: 0 811 2193
Size: 811 1382
BbeI G_GCGC'C
Cuts at: 0 521 2193
Size: 521 1672
BbsI GAAGACnn'nnnn_
Cuts at: 0 1545 2193
Size: 1545 648
BglI GCCn_nnn'nGGC
Cuts at: 0 850 2193
Size: 850 1343
BglII A'GATC_T
Cuts at: 0 1569 1942 2193
Size: 1569 373 251
Fragments arranged by size:
1569 373 251
Bme1580I G_kGCm'C
Cuts at: 0 316 2193
Size: 316 1877
BmrI ACTGGGnnnn_n'
Cuts at: 0 1069 1590 2193
Size: 1069 521 603
Fragments arranged by size:
1069 603 521
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 414 543 752 1733 1947 2193
Size: 414 129 209 981 214 246
Fragments arranged by size:
981 414 246 214 209 129
BsaAI yAC'GTr
Cuts at: 0 570 2193
Size: 570 1623
BsaBI GATnn'nnATC
Cuts at: 0 1947 2193
Size: 1947 246
BsaHI Gr'CG_yC
Cuts at: 0 518 1156 2193
Size: 518 638 1037
Fragments arranged by size:
1037 638 518
BsaWI w'CCGG_w
Cuts at: 0 1627 2193
Size: 1627 566
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 601 673 2193
Size: 601 72 1520
Fragments arranged by size:
1520 601 72
BseYI C'CCAG_C
Cuts at: 0 512 847 2193
Size: 512 335 1346
Fragments arranged by size:
1346 512 335
BsiHKAI G_wGCw'C
Cuts at: 0 316 452 811 2193
Size: 316 136 359 1382
Fragments arranged by size:
1382 359 316 136
BsmI GAATG_Cn'
Cuts at: 0 1657 2193
Size: 1657 536
Bsp1286I G_dGCh'C
Cuts at: 0 316 452 811 2193
Size: 316 136 359 1382
Fragments arranged by size:
1382 359 316 136
BspHI T'CATG_A
Cuts at: 0 125 236 1548 2193
Size: 125 111 1312 645
Fragments arranged by size:
1312 645 125 111
BssSI C'ACGA_G
Cuts at: 0 613 2193
Size: 613 1580
BstEII G'GTnAC_C
Cuts at: 0 252 2193
Size: 252 1941
BstXI CCAn_nnnn'nTGG
Cuts at: 0 210 1830 2193
Size: 210 1620 363
Fragments arranged by size:
1620 363 210
BstYI r'GATC_y
Cuts at: 0 1569 1942 2193
Size: 1569 373 251
Fragments arranged by size:
1569 373 251
BtgI C'CryG_G
Cuts at: 0 699 2193
Size: 699 1494
BtsI GCAGTG_nn'
Cuts at: 0 280 1157 2193
Size: 280 877 1036
Fragments arranged by size:
1036 877 280
ClaI AT'CG_AT
Cuts at: 0 1776 2193
Size: 1776 417
DraI TTT'AAA
Cuts at: 0 77 2193
Size: 77 2116
DraIII CAC_nnn'GTG
Cuts at: 0 990 2193
Size: 990 1203
EaeI y'GGCC_r
Cuts at: 0 277 1058 1791 2193
Size: 277 781 733 402
Fragments arranged by size:
781 733 402 277
EarI CTCTTCn'nnn_
Cuts at: 0 1547 1877 2193
Size: 1547 330 316
Fragments arranged by size:
1547 330 316
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 71 2193
Size: 71 2122
EcoICRI GAG'CTC
Cuts at: 0 809 2193
Size: 809 1384
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 414 543 752 875 1544 1733 1947 2193
Size: 414 129 209 123 669 189 214 246
Fragments arranged by size:
669 414 246 214 209 189 129 123
EcoO109I rG'GnC_Cy
Cuts at: 0 288 345 719 2193
Size: 288 57 374 1474
Fragments arranged by size:
1474 374 288 57
EcoRI G'AATT_C
Cuts at: 0 1192 2193
Size: 1192 1001
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 979 2193
Size: 979 1214
FspI TGC'GCA
Cuts at: 0 529 2193
Size: 529 1664
FspAI rTGC'GCAy
Cuts at: 0 529 2193
Size: 529 1664
HaeII r_GCGC'y
Cuts at: 0 499 521 789 930 949 2193
Size: 499 22 268 141 19 1244
Fragments arranged by size:
1244 499 268 141 22 19
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1659 1691 1804 1836 1885 1917 2193
Size: 1659 32 113 32 49 32 276
Fragments arranged by size:
1659 276 113 49 32 32 32
HindIII A'AGCT_T
Cuts at: 0 2156 2193
Size: 2156 37
KasI G'GCGC_C
Cuts at: 0 517 2193
Size: 517 1676
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 704 836 1478 2193
Size: 704 132 642 715
Fragments arranged by size:
715 704 642 132
MscI TGG'CCA
Cuts at: 0 279 1060 1793 2193
Size: 279 781 733 400
Fragments arranged by size:
781 733 400 279
MslI CAynn'nnrTG
Cuts at: 0 208 262 407 979 1828 1840 2193
Size: 208 54 145 572 849 12 353
Fragments arranged by size:
849 572 353 208 145 54 12
MspA1I CmG'CkG
Cuts at: 0 504 841 847 2193
Size: 504 337 6 1346
Fragments arranged by size:
1346 504 337 6
NarI GG'CG_CC
Cuts at: 0 518 2193
Size: 518 1675
NdeI CA'TA_TG
Cuts at: 0 1146 2193
Size: 1146 1047
NspI r_CATG'y
Cuts at: 0 122 2193
Size: 122 2071
PasI CC'CwG_GG
Cuts at: 0 536 2193
Size: 536 1657
PciI A'CATG_T
Cuts at: 0 118 2193
Size: 118 2075
PflMI CCAn_nnn'nTGG
Cuts at: 0 1097 2193
Size: 1097 1096
PfoI T'CCnGG_A
Cuts at: 0 1963 2193
Size: 1963 230
PpuMI rG'GwC_Cy
Cuts at: 0 288 719 2193
Size: 288 431 1474
Fragments arranged by size:
1474 431 288
PsiI TTA'TAA
Cuts at: 0 1229 2193
Size: 1229 964
PstI C_TGCA'G
Cuts at: 0 865 1135 2193
Size: 865 270 1058
Fragments arranged by size:
1058 865 270
RsrII CG'GwC_CG
Cuts at: 0 826 2193
Size: 826 1367
SacI G_AGCT'C
Cuts at: 0 811 2193
Size: 811 1382
SanDI GG'GwC_CC
Cuts at: 0 719 2193
Size: 719 1474
SbfI CC_TGCA'GG
Cuts at: 0 865 2193
Size: 865 1328
SfcI C'TryA_G
Cuts at: 0 861 1131 1688 2193
Size: 861 270 557 505
Fragments arranged by size:
861 557 505 270
SfoI GGC'GCC
Cuts at: 0 519 2193
Size: 519 1674
SnaBI TAC'GTA
Cuts at: 0 570 2193
Size: 570 1623
StuI AGG'CCT
Cuts at: 0 1888 2193
Size: 1888 305
StyI C'CwwG_G
Cuts at: 0 140 1025 1889 1995 2193
Size: 140 885 864 106 198
Fragments arranged by size:
885 864 198 140 106
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 599 631 2022 2054 2193
Size: 599 32 1391 32 139
Fragments arranged by size:
1391 599 139 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 1671 2193
Size: 1671 522
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1111 1216 1380 2193
Size: 1111 105 164 813
Fragments arranged by size:
1111 813 164 105
XmnI GAAnn'nnTTC
Cuts at: 0 928 2193
Size: 928 1265
Enzymes that do cut:
AcuI AfeI AflIII AhdI AleI ApaLI ApoI AseI
AvaI AvrII BanI BanII BbeI BbsI BglI BglII
Bme1580I BmrI BpmI BsaAI BsaBI BsaHI BsaWI BseRI
BseYI BsiHKAI BsmI Bsp1286I BspHI BssSI BstEII BstXI
BstYI BtgI BtsI ClaI DraI DraIII EaeI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI FspAI
HaeII Hin4I HindIII KasI MmeI MscI MslI MspA1I
NarI NdeI NspI PasI PciI PflMI PfoI PpuMI
PsiI PstI RsrII SacI SanDI SbfI SfcI SfoI
SnaBI StuI StyI TstI Tth111I XcmI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AflII AgeI AlfI
AloI AlwNI ApaI AscI AsiSI BaeI BamHI BbvCI
BcgI BciVI BclI BfrBI BlpI BmgBI BmtI BplI
Bpu10I BpuEI BsaI BsaXI BsgI BsiEI BsiWI BsmBI
BspEI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BstAPI
BstBI BstZ17I Bsu36I BtgZI CspCI DrdI EagI EcoNI
EcoRV FseI HincII HpaI KpnI MfeI MluI NaeI
NcoI NgoMIV NheI NotI NruI NsiI PacI PmeI
PmlI PpiI PshAI PspOMI PspXI PsrI PvuI PvuII
SacII SalI SapI ScaI SexAI SfiI SgfI SgrAI
SmaI SmlI SpeI SphI SrfI SspI SwaI TaqII
TatI XbaI XhoI XmaI ZraI