Restriction map for
pFN21AE9457
(Linear) (Six-base) MAPSORT of: KIEE9457.seq Check: 5239 from: 1 to: 3963
KIEE9457.orf
With 182 enzymes: SgfI *
December 27, 2011 16:40 ..
AccI GT'mk_AC
Cuts at: 0 1645 3963
Size: 1645 2318
Acc65I G'GTAC_C
Cuts at: 0 3907 3963
Size: 3907 56
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 307 1065 1537 3096 3963
Size: 307 758 472 1559 867
Fragments arranged by size:
1559 867 758 472 307
AfeI AGC'GCT
Cuts at: 0 68 3963
Size: 68 3895
AflII C'TTAA_G
Cuts at: 0 2854 3963
Size: 2854 1109
AflIII A'CryG_T
Cuts at: 0 1494 3963
Size: 1494 2469
AhdI GACnn_n'nnGTC
Cuts at: 0 2069 3553 3963
Size: 2069 1484 410
Fragments arranged by size:
2069 1484 410
AleI CACnn'nnGTG
Cuts at: 0 679 3137 3963
Size: 679 2458 826
Fragments arranged by size:
2458 826 679
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2739 3279 3377 3387 3963
Size: 2739 540 98 10 576
Fragments arranged by size:
2739 576 540 98 10
AlwNI CAG_nnn'CTG
Cuts at: 0 219 693 1332 1696 1948 2095 2471 3963
Size: 219 474 639 364 252 147 376 1492
Fragments arranged by size:
1492 639 474 376 364 252 219 147
ApaLI G'TGCA_C
Cuts at: 0 3496 3963
Size: 3496 467
ApoI r'AATT_y
Cuts at: 0 486 547 1405 1953 1962 3321 3753 3963
Size: 486 61 858 548 9 1359 432 210
Fragments arranged by size:
1359 858 548 486 432 210 61 9
AvaI C'yCGr_G
Cuts at: 0 146 3651 3963
Size: 146 3505 312
Fragments arranged by size:
3505 312 146
BamHI G'GATC_C
Cuts at: 0 1731 2586 3963
Size: 1731 855 1377
Fragments arranged by size:
1731 1377 855
BanI G'GyrC_C
Cuts at: 0 103 169 367 2156 3255 3465 3907 3963
Size: 103 66 198 1789 1099 210 442 56
Fragments arranged by size:
1789 1099 442 210 198 103 66 56
BanII G_rGCy'C
Cuts at: 0 1590 2864 3199 3963
Size: 1590 1274 335 764
Fragments arranged by size:
1590 1274 764 335
BbeI G_GCGC'C
Cuts at: 0 107 173 3469 3963
Size: 107 66 3296 494
Fragments arranged by size:
3296 494 107 66
BbsI GAAGACnn'nnnn_
Cuts at: 0 1543 3278 3963
Size: 1543 1735 685
Fragments arranged by size:
1735 1543 685
BbvCI CC'TCA_GC
Cuts at: 0 3407 3963
Size: 3407 556
BciVI GTATCCnnnnn_n'
Cuts at: 0 624 2659 3963
Size: 624 2035 1304
Fragments arranged by size:
2035 1304 624
BclI T'GATC_A
Cuts at: 0 807 3963
Size: 807 3156
BfrBI ATG'CAT
Cuts at: 0 594 3963
Size: 594 3369
BglI GCCn_nnn'nGGC
Cuts at: 0 23 3632 3963
Size: 23 3609 331
Fragments arranged by size:
3609 331 23
BlpI GC'TnA_GC
Cuts at: 0 1759 3963
Size: 1759 2204
Bme1580I G_kGCm'C
Cuts at: 0 924 3500 3963
Size: 924 2576 463
Fragments arranged by size:
2576 924 463
BmgBI CAC'GTC
Cuts at: 0 1452 3963
Size: 1452 2511
BmtI G_CTAG'C
Cuts at: 0 1579 3963
Size: 1579 2384
Bpu10I CC'TnA_GC
Cuts at: 0 2837 3407 3963
Size: 2837 570 556
Fragments arranged by size:
2837 570 556
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 491 2359 3486 3963
Size: 491 1868 1127 477
Fragments arranged by size:
1868 1127 491 477
BsaAI yAC'GTr
Cuts at: 0 3462 3963
Size: 3462 501
BsaBI GATnn'nnATC
Cuts at: 0 2365 3963
Size: 2365 1598
BsaHI Gr'CG_yC
Cuts at: 0 15 104 170 3466 3963
Size: 15 89 66 3296 497
Fragments arranged by size:
3296 497 89 66 15
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 889 919 1577 1607 3963
Size: 889 30 658 30 2356
Fragments arranged by size:
2356 889 658 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 2299 2884 3669 3963
Size: 2299 585 785 294
Fragments arranged by size:
2299 785 585 294
BseYI C'CCAG_C
Cuts at: 0 81 599 3630 3704 3963
Size: 81 518 3031 74 259
Fragments arranged by size:
3031 518 259 81 74
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 213 1019 1564 3625 3963
Size: 213 806 545 2061 338
Fragments arranged by size:
2061 806 545 338 213
BsiHKAI G_wGCw'C
Cuts at: 0 1590 2969 3199 3500 3963
Size: 1590 1379 230 301 463
Fragments arranged by size:
1590 1379 463 301 230
BsmI GAATG_Cn'
Cuts at: 0 438 1918 2466 3963
Size: 438 1480 548 1497
Fragments arranged by size:
1497 1480 548 438
Bsp1286I G_dGCh'C
Cuts at: 0 924 1590 2864 2969 3199 3500 3963
Size: 924 666 1274 105 230 301 463
Fragments arranged by size:
1274 924 666 463 301 230 105
BspHI T'CATG_A
Cuts at: 0 3173 3963
Size: 3173 790
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 204 3963
Size: 204 3759
BsrBI CCG'CTC
Cuts at: 0 54 3963
Size: 54 3909
BsrDI GCAATG_nn'
Cuts at: 0 1463 1559 1779 1913 1932 3888 3930 3963
Size: 1463 96 220 134 19 1956 42 33
Fragments arranged by size:
1956 1463 220 134 96 42 33 19
BsrFI r'CCGG_y
Cuts at: 0 17 121 1571 1579 3626 3963
Size: 17 104 1450 8 2047 337
Fragments arranged by size:
2047 1450 337 104 17 8
BssHII G'CGCG_C
Cuts at: 0 29 3963
Size: 29 3934
BstAPI GCAn_nnn'nTGC
Cuts at: 0 219 3963
Size: 219 3744
BstBI TT'CG_AA
Cuts at: 0 3486 3963
Size: 3486 477
BstEII G'GTnAC_C
Cuts at: 0 249 1995 3963
Size: 249 1746 1968
Fragments arranged by size:
1968 1746 249
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1098 1147 1311 2482 3963
Size: 1098 49 164 1171 1481
Fragments arranged by size:
1481 1171 1098 164 49
BstYI r'GATC_y
Cuts at: 0 360 402 949 1731 2242 2586 3963
Size: 360 42 547 782 511 344 1377
Fragments arranged by size:
1377 782 547 511 360 344 42
BtgI C'CryG_G
Cuts at: 0 107 1140 1310 2864 3963
Size: 107 1033 170 1554 1099
Fragments arranged by size:
1554 1099 1033 170 107
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 580 733 3734 3963
Size: 580 153 3001 229
Fragments arranged by size:
3001 580 229 153
BtsI GCAGTG_nn'
Cuts at: 0 655 1129 1152 1552 2065 3282 3963
Size: 655 474 23 400 513 1217 681
Fragments arranged by size:
1217 681 655 513 474 400 23
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 808 843 3572 3607 3963
Size: 808 35 2729 35 356
Fragments arranged by size:
2729 808 356 35 35
EaeI y'GGCC_r
Cuts at: 0 2264 2707 3854 3963
Size: 2264 443 1147 109
Fragments arranged by size:
2264 1147 443 109
EarI CTCTTCn'nnn_
Cuts at: 0 3657 3963
Size: 3657 306
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1037 3963
Size: 1037 2926
EcoICRI GAG'CTC
Cuts at: 0 1588 3197 3963
Size: 1588 1609 766
Fragments arranged by size:
1609 1588 766
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 307 1065 1537 3096 3963
Size: 307 758 472 1559 867
Fragments arranged by size:
1559 867 758 472 307
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1533 3963
Size: 1533 2430
EcoO109I rG'GnC_Cy
Cuts at: 0 216 3895 3963
Size: 216 3679 68
Fragments arranged by size:
3679 216 68
EcoRI G'AATT_C
Cuts at: 0 547 3963
Size: 547 3416
EcoRV GAT'ATC
Cuts at: 0 278 3963
Size: 278 3685
HaeII r_GCGC'y
Cuts at: 0 70 107 115 173 3469 3963
Size: 70 37 8 58 3296 494
Fragments arranged by size:
3296 494 70 58 37 8
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 317 349 399 431 546 578 729 761
Size: 317 32 50 32 115 32 151 32
Cuts at: 761 1417 1449 1716 1748 2055 2087 2606 2638
Size: 656 32 267 32 307 32 519 32
Cuts at: 2638 2911 2943 3539 3571 3887 3919 3963
Size: 273 32 596 32 316 32 44
Fragments arranged by size:
656 596 519 317 316 307 273 267
151 115 50 44 32 32 32 32
32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 0 698 1461 3216 3963
Size: 698 763 1755 747
Fragments arranged by size:
1755 763 747 698
HindIII A'AGCT_T
Cuts at: 0 1184 1628 1903 1933 3108 3312 3963
Size: 1184 444 275 30 1175 204 651
Fragments arranged by size:
1184 1175 651 444 275 204 30
KasI G'GCGC_C
Cuts at: 0 103 169 3465 3963
Size: 103 66 3296 498
Fragments arranged by size:
3296 498 103 66
KpnI G_GTAC'C
Cuts at: 0 3911 3963
Size: 3911 52
MscI TGG'CCA
Cuts at: 0 3856 3963
Size: 3856 107
MslI CAynn'nnrTG
Cuts at: 0 679 820 1145 1660 2392 3137 3261 3963
Size: 679 141 325 515 732 745 124 702
Fragments arranged by size:
745 732 702 679 515 325 141 124
MspA1I CmG'CkG
Cuts at: 0 42 109 1758 3808 3963
Size: 42 67 1649 2050 155
Fragments arranged by size:
2050 1649 155 67 42
NaeI GCC'GGC
Cuts at: 0 19 123 3628 3963
Size: 19 104 3505 335
Fragments arranged by size:
3505 335 104 19
NarI GG'CG_CC
Cuts at: 0 104 170 3466 3963
Size: 104 66 3296 497
Fragments arranged by size:
3296 497 104 66
NcoI C'CATG_G
Cuts at: 0 1140 3963
Size: 1140 2823
NgoMIV G'CCGG_C
Cuts at: 0 17 121 3626 3963
Size: 17 104 3505 337
Fragments arranged by size:
3505 337 104 17
NheI G'CTAG_C
Cuts at: 0 1575 3963
Size: 1575 2388
NsiI A_TGCA'T
Cuts at: 0 596 3963
Size: 596 3367
NspI r_CATG'y
Cuts at: 0 704 819 2391 2799 3963
Size: 704 115 1572 408 1164
Fragments arranged by size:
1572 1164 704 408 115
PasI CC'CwG_GG
Cuts at: 0 3330 3963
Size: 3330 633
PflMI CCAn_nnn'nTGG
Cuts at: 0 792 1686 1948 3963
Size: 792 894 262 2015
Fragments arranged by size:
2015 894 792 262
PfoI T'CCnGG_A
Cuts at: 0 1158 3963
Size: 1158 2805
PmlI CAC'GTG
Cuts at: 0 3462 3963
Size: 3462 501
PpuMI rG'GwC_Cy
Cuts at: 0 216 3895 3963
Size: 216 3679 68
Fragments arranged by size:
3679 216 68
PspXI vC'TCGA_Gb
Cuts at: 0 3651 3963
Size: 3651 312
PstI C_TGCA'G
Cuts at: 0 662 1124 1534 2679 3366 3963
Size: 662 462 410 1145 687 597
Fragments arranged by size:
1145 687 662 597 462 410
PvuII CAG'CTG
Cuts at: 0 1758 3808 3963
Size: 1758 2050 155
Fragments arranged by size:
2050 1758 155
SacI G_AGCT'C
Cuts at: 0 1590 3199 3963
Size: 1590 1609 764
Fragments arranged by size:
1609 1590 764
SacII CC_GC'GG
Cuts at: 0 110 3963
Size: 110 3853
SanDI GG'GwC_CC
Cuts at: 0 3895 3963
Size: 3895 68
SapI GCTCTTCn'nnn_
Cuts at: 0 3657 3963
Size: 3657 306
ScaI AGT'ACT
Cuts at: 0 1042 2519 3963
Size: 1042 1477 1444
Fragments arranged by size:
1477 1444 1042
SexAI A'CCwGG_T
Cuts at: 0 634 3963
Size: 634 3329
SfcI C'TryA_G
Cuts at: 0 658 1120 1530 2675 3362 3678 3963
Size: 658 462 410 1145 687 316 285
Fragments arranged by size:
1145 687 658 462 410 316 285
SfoI GGC'GCC
Cuts at: 0 105 171 3467 3963
Size: 105 66 3296 496
Fragments arranged by size:
3296 496 105 66
SgrAI Cr'CCGG_yG
Cuts at: 0 17 3963
Size: 17 3946
SmlI C'TyrA_G
Cuts at: 0 506 2374 2854 3501 3651 3963
Size: 506 1868 480 647 150 312
Fragments arranged by size:
1868 647 506 480 312 150
SpeI A'CTAG_T
Cuts at: 0 3778 3963
Size: 3778 185
SspI AAT'ATT
Cuts at: 0 939 1617 3963
Size: 939 678 2346
Fragments arranged by size:
2346 939 678
StyI C'CwwG_G
Cuts at: 0 1140 1421 2747 2954 3963
Size: 1140 281 1326 207 1009
Fragments arranged by size:
1326 1140 1009 281 207
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1153 1626 2144 2272 3273 3963
Size: 1153 473 518 128 1001 690
Fragments arranged by size:
1153 1001 690 518 473 128
TatI w'GTAC_w
Cuts at: 0 1040 2517 2798 3692 3963
Size: 1040 1477 281 894 271
Fragments arranged by size:
1477 1040 894 281 271
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 622 654 889 921 3963
Size: 622 32 235 32 3042
Fragments arranged by size:
3042 622 235 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 371 985 1620 3963
Size: 371 614 635 2343
Fragments arranged by size:
2343 635 614 371
XhoI C'TCGA_G
Cuts at: 0 3651 3963
Size: 3651 312
Enzymes that do cut:
AccI Acc65I AcuI AfeI AflII AflIII AhdI AleI
AlfI AlwNI ApaLI ApoI AvaI BamHI BanI BanII
BbeI BbsI BbvCI BciVI BclI BfrBI BglI BlpI
Bme1580I BmgBI BmtI Bpu10I BpuEI BsaAI BsaBI BsaHI
BsaXI BseRI BseYI BsgI BsiHKAI BsmI Bsp1286I BspHI
BspMI BsrBI BsrDI BsrFI BssHII BstAPI BstBI BstEII
BstXI BstYI BtgI BtgZI BtsI CspCI EaeI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV HaeII
Hin4I HincII HindIII KasI KpnI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NheI NsiI NspI PasI
PflMI PfoI PmlI PpuMI PspXI PstI PvuII SacI
SacII SanDI SapI ScaI SexAI SfcI SfoI SgrAI
SmlI SpeI SspI StyI TaqII TatI TstI XcmI
XhoI
Enzymes that do not cut:
AarI AatII AclI AgeI AloI ApaI AscI AseI
AsiSI AvrII BaeI BcgI BglII BmrI BplI BpmI
BsaI BsaWI BsiEI BsiWI BsmBI BspEI BsrGI BssSI
BstZ17I Bsu36I ClaI DraI DraIII DrdI EagI FalI
FseI FspI FspAI HpaI MfeI MluI MmeI NdeI
NotI NruI PacI PciI PmeI PpiI PshAI PsiI
PspOMI PsrI PvuI RsrII SalI SbfI SfiI SgfI
SmaI SnaBI SphI SrfI StuI SwaI Tth111I XbaI
XmaI XmnI ZraI