Restriction map for
pFN21AE9451
(Linear) (Six-base) MAPSORT of: KIEE9451.seq Check: 4726 from: 1 to: 2463
KIEE9451.orf
With 182 enzymes: SgfI *
March 22, 2012 18:29 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 742 2260 2463
Size: 742 1518 203
Fragments arranged by size:
1518 742 203
AatII G_ACGT'C
Cuts at: 0 1195 2463
Size: 1195 1268
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 921 1053 1138 1546 1896 1915 2463
Size: 921 132 85 408 350 19 548
Fragments arranged by size:
921 548 408 350 132 85 19
AfeI AGC'GCT
Cuts at: 0 1477 2463
Size: 1477 986
AleI CACnn'nnGTG
Cuts at: 0 1429 1666 1943 2463
Size: 1429 237 277 520
Fragments arranged by size:
1429 520 277 237
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1961 2463
Size: 1961 502
AlwNI CAG_nnn'CTG
Cuts at: 0 422 2463
Size: 422 2041
ApaLI G'TGCA_C
Cuts at: 0 1049 2463
Size: 1049 1414
ApoI r'AATT_y
Cuts at: 0 771 1174 1533 2463
Size: 771 403 359 930
Fragments arranged by size:
930 771 403 359
AvaI C'yCGr_G
Cuts at: 0 1538 2463
Size: 1538 925
BamHI G'GATC_C
Cuts at: 0 216 2463
Size: 216 2247
BanII G_rGCy'C
Cuts at: 0 871 1787 2463
Size: 871 916 676
Fragments arranged by size:
916 871 676
BbsI GAAGACnn'nnnn_
Cuts at: 0 518 1203 2395 2463
Size: 518 685 1192 68
Fragments arranged by size:
1192 685 518 68
BbvCI CC'TCA_GC
Cuts at: 0 1260 2463
Size: 1260 1203
BciVI GTATCCnnnnn_n'
Cuts at: 0 2120 2463
Size: 2120 343
BclI T'GATC_A
Cuts at: 0 2093 2463
Size: 2093 370
BfrBI ATG'CAT
Cuts at: 0 2019 2463
Size: 2019 444
BglI GCCn_nnn'nGGC
Cuts at: 0 743 2463
Size: 743 1720
Bme1580I G_kGCm'C
Cuts at: 0 62 124 688 1053 2463
Size: 62 62 564 365 1410
Fragments arranged by size:
1410 564 365 62 62
BmgBI CAC'GTC
Cuts at: 0 516 2463
Size: 516 1947
BmrI ACTGGGnnnn_n'
Cuts at: 0 366 2393 2400 2463
Size: 366 2027 7 63
Fragments arranged by size:
2027 366 63 7
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1212 2463
Size: 1212 1251
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1237 1583 1627 1677 2079 2463
Size: 1237 346 44 50 402 384
Fragments arranged by size:
1237 402 384 346 50 44
Bpu10I CC'TnA_GC
Cuts at: 0 428 1260 2463
Size: 428 832 1203
Fragments arranged by size:
1203 832 428
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 648 2403 2463
Size: 648 1755 60
Fragments arranged by size:
1755 648 60
BsaI GGTCTCn'nnnn_
Cuts at: 0 646 1414 2463
Size: 646 768 1049
Fragments arranged by size:
1049 768 646
BsaAI yAC'GTr
Cuts at: 0 1166 2463
Size: 1166 1297
BsaBI GATnn'nnATC
Cuts at: 0 609 1381 1686 2463
Size: 609 772 305 777
Fragments arranged by size:
777 772 609 305
BsaHI Gr'CG_yC
Cuts at: 0 1192 1774 2463
Size: 1192 582 689
Fragments arranged by size:
1192 689 582
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 696 726 2463
Size: 696 30 1737
Fragments arranged by size:
1737 696 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 10 2463
Size: 10 2453
BseYI C'CCAG_C
Cuts at: 0 50 123 263 2210 2241 2398 2463
Size: 50 73 140 1947 31 157 65
Fragments arranged by size:
1947 157 140 73 65 50 31
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 336 548 837 1804 1921 2017 2463
Size: 336 212 289 967 117 96 446
Fragments arranged by size:
967 446 336 289 212 117 96
BsiEI CG_ry'CG
Cuts at: 0 1106 2463
Size: 1106 1357
BsiHKAI G_wGCw'C
Cuts at: 0 1053 1663 1787 2463
Size: 1053 610 124 676
Fragments arranged by size:
1053 676 610 124
BsmI GAATG_Cn'
Cuts at: 0 432 676 1333 2021 2463
Size: 432 244 657 688 442
Fragments arranged by size:
688 657 442 432 244
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1183 2463
Size: 1183 1280
Bsp1286I G_dGCh'C
Cuts at: 0 62 124 688 871 1053 1663 1787 2463
Size: 62 62 564 183 182 610 124 676
Fragments arranged by size:
676 610 564 183 182 124 62 62
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 23 742 1038 2260 2463
Size: 23 719 296 1222 203
Fragments arranged by size:
1222 719 296 203 23
BsrDI GCAATG_nn'
Cuts at: 0 201 765 887 1009 2463
Size: 201 564 122 122 1454
Fragments arranged by size:
1454 564 201 122 122
BstAPI GCAn_nnn'nTGC
Cuts at: 0 422 1759 2463
Size: 422 1337 704
Fragments arranged by size:
1337 704 422
BstXI CCAn_nnnn'nTGG
Cuts at: 0 150 271 864 928 1431 1770 2463
Size: 150 121 593 64 503 339 693
Fragments arranged by size:
693 593 503 339 150 121 64
BstYI r'GATC_y
Cuts at: 0 216 531 822 995 1587 2423 2463
Size: 216 315 291 173 592 836 40
Fragments arranged by size:
836 592 315 291 216 173 40
BtgI C'CryG_G
Cuts at: 0 452 2463
Size: 452 2011
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 243 1791 2463
Size: 243 1548 672
Fragments arranged by size:
1548 672 243
BtsI GCAGTG_nn'
Cuts at: 0 624 1028 1338 2463
Size: 624 404 310 1125
Fragments arranged by size:
1125 624 404 310
DraI TTT'AAA
Cuts at: 0 1469 2463
Size: 1469 994
EaeI y'GGCC_r
Cuts at: 0 621 1202 2349 2463
Size: 621 581 1147 114
Fragments arranged by size:
1147 621 581 114
EarI CTCTTCn'nnn_
Cuts at: 0 28 342 961 1181 1331 2221 2463
Size: 28 314 619 220 150 890 242
Fragments arranged by size:
890 619 314 242 220 150 28
EcoICRI GAG'CTC
Cuts at: 0 1785 2463
Size: 1785 678
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 921 1053 1138 1237 1546 1583 1627 1677
Size: 921 132 85 99 309 37 44 50
Cuts at: 1677 1896 1915 2079 2463
Size: 219 19 164 384
Fragments arranged by size:
921 384 309 219 164 132 99 85
50 44 37 19
EcoNI CCTnn'n_nnAGG
Cuts at: 0 2267 2463
Size: 2267 196
EcoO109I rG'GnC_Cy
Cuts at: 0 360 2315 2463
Size: 360 1955 148
Fragments arranged by size:
1955 360 148
EcoRI G'AATT_C
Cuts at: 0 1533 2463
Size: 1533 930
EcoRV GAT'ATC
Cuts at: 0 396 2463
Size: 396 2067
HaeII r_GCGC'y
Cuts at: 0 1479 2463
Size: 1479 984
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 319 351 1521 1553 1567 1599 1740 1772
Size: 319 32 1170 32 14 32 141 32
Cuts at: 1772 2463
Size: 691
Fragments arranged by size:
1170 691 319 141 32 32 32 32
14
HincII GTy'rAC
Cuts at: 0 447 2463
Size: 447 2016
HindIII A'AGCT_T
Cuts at: 0 1712 2463
Size: 1712 751
MfeI C'AATT_G
Cuts at: 0 1397 2463
Size: 1397 1066
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 462 555 1331 2463
Size: 462 93 776 1132
Fragments arranged by size:
1132 776 462 93
MscI TGG'CCA
Cuts at: 0 623 1204 2463
Size: 623 581 1259
Fragments arranged by size:
1259 623 581
MslI CAynn'nnrTG
Cuts at: 0 679 1429 1666 1768 1930 1943 2463
Size: 679 750 237 102 162 13 520
Fragments arranged by size:
750 679 520 237 162 102 13
MspA1I CmG'CkG
Cuts at: 0 50 320 473 878 941 1739 2463
Size: 50 270 153 405 63 798 724
Fragments arranged by size:
798 724 405 270 153 63 50
NcoI C'CATG_G
Cuts at: 0 452 2463
Size: 452 2011
NdeI CA'TA_TG
Cuts at: 0 1035 2463
Size: 1035 1428
NsiI A_TGCA'T
Cuts at: 0 2021 2463
Size: 2021 442
NspI r_CATG'y
Cuts at: 0 556 704 2463
Size: 556 148 1759
Fragments arranged by size:
1759 556 148
PasI CC'CwG_GG
Cuts at: 0 864 2463
Size: 864 1599
PflMI CCAn_nnn'nTGG
Cuts at: 0 760 789 2463
Size: 760 29 1674
Fragments arranged by size:
1674 760 29
PfoI T'CCnGG_A
Cuts at: 0 219 1214 1654 1706 2463
Size: 219 995 440 52 757
Fragments arranged by size:
995 757 440 219 52
PmlI CAC'GTG
Cuts at: 0 1166 2463
Size: 1166 1297
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2035 2067 2463
Size: 2035 32 396
Fragments arranged by size:
2035 396 32
PpuMI rG'GwC_Cy
Cuts at: 0 360 2463
Size: 360 2103
PstI C_TGCA'G
Cuts at: 0 1738 2312 2463
Size: 1738 574 151
Fragments arranged by size:
1738 574 151
PvuII CAG'CTG
Cuts at: 0 50 320 473 878 941 1739 2463
Size: 50 270 153 405 63 798 724
Fragments arranged by size:
798 724 405 270 153 63 50
SacI G_AGCT'C
Cuts at: 0 1787 2463
Size: 1787 676
SanDI GG'GwC_CC
Cuts at: 0 360 2463
Size: 360 2103
SapI GCTCTTCn'nnn_
Cuts at: 0 2221 2463
Size: 2221 242
SbfI CC_TGCA'GG
Cuts at: 0 2312 2463
Size: 2312 151
SexAI A'CCwGG_T
Cuts at: 0 2182 2463
Size: 2182 281
SfcI C'TryA_G
Cuts at: 0 102 462 873 1734 2308 2463
Size: 102 360 411 861 574 155
Fragments arranged by size:
861 574 411 360 155 102
SmlI C'TyrA_G
Cuts at: 0 627 1538 2418 2463
Size: 627 911 880 45
Fragments arranged by size:
911 880 627 45
SphI G_CATG'C
Cuts at: 0 556 2463
Size: 556 1907
StuI AGG'CCT
Cuts at: 0 2203 2463
Size: 2203 260
StyI C'CwwG_G
Cuts at: 0 452 783 1181 1364 2463
Size: 452 331 398 183 1099
Fragments arranged by size:
1099 452 398 331 183
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 2226 2463
Size: 2226 237
TatI w'GTAC_w
Cuts at: 0 388 2463
Size: 388 2075
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 696 728 1007 1039 1072 1104 2463
Size: 696 32 279 32 33 32 1359
Fragments arranged by size:
1359 696 279 33 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 358 442 2463
Size: 358 84 2021
Fragments arranged by size:
2021 358 84
XbaI T'CTAG_A
Cuts at: 0 493 2463
Size: 493 1970
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1212 2463
Size: 1212 1251
XhoI C'TCGA_G
Cuts at: 0 1538 2463
Size: 1538 925
XmnI GAAnn'nnTTC
Cuts at: 0 1909 2463
Size: 1909 554
ZraI GAC'GTC
Cuts at: 0 1193 2463
Size: 1193 1270
Enzymes that do cut:
AarI AatII AcuI AfeI AleI AlfI AlwNI ApaLI
ApoI AvaI BamHI BanII BbsI BbvCI BciVI BclI
BfrBI BglI Bme1580I BmgBI BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspMI BsrDI
BstAPI BstXI BstYI BtgI BtgZI BtsI DraI EaeI
EarI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV HaeII
Hin4I HincII HindIII MfeI MmeI MscI MslI MspA1I
NcoI NdeI NsiI NspI PasI PflMI PfoI PmlI
PpiI PpuMI PstI PvuII SacI SanDI SapI SbfI
SexAI SfcI SmlI SphI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmnI ZraI
Enzymes that do not cut:
AccI Acc65I AclI AflII AflIII AgeI AhdI AloI
ApaI AscI AseI AsiSI AvrII BaeI BanI BbeI
BcgI BglII BlpI BmtI BsaWI BsiWI BspEI BspHI
BsrBI BsrFI BsrGI BssHII BssSI BstBI BstEII BstZ17I
Bsu36I ClaI CspCI DraIII DrdI EagI EciI FalI
FseI FspI FspAI HpaI KasI KpnI MluI NaeI
NarI NgoMIV NheI NotI NruI PacI PciI PmeI
PshAI PsiI PspOMI PspXI PsrI PvuI RsrII SacII
SalI ScaI SfiI SfoI SgfI SgrAI SmaI SnaBI
SpeI SrfI SspI SwaI XmaI