Restriction map for
pFN21AE9450
(Linear) (Six-base) MAPSORT of: KIEE9450.seq Check: 3465 from: 1 to: 4752
KIEE9450.orf
With 182 enzymes: SgfI *
December 27, 2011 16:40 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 868 1114 1312 3869 4752
Size: 868 246 198 2557 883
Fragments arranged by size:
2557 883 868 246 198
AatII G_ACGT'C
Cuts at: 0 2252 4752
Size: 2252 2500
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 3403 3778 3811 4752
Size: 3403 375 33 941
Fragments arranged by size:
3403 941 375 33
AfeI AGC'GCT
Cuts at: 0 3154 4752
Size: 3154 1598
AflIII A'CryG_T
Cuts at: 0 581 1471 1609 2621 2906 3845 4481 4752
Size: 581 890 138 1012 285 939 636 271
Fragments arranged by size:
1012 939 890 636 581 285 271 138
AgeI A'CCGG_T
Cuts at: 0 1054 4752
Size: 1054 3698
AhdI GACnn_n'nnGTC
Cuts at: 0 2049 4752
Size: 2049 2703
AleI CACnn'nnGTG
Cuts at: 0 136 3037 4627 4752
Size: 136 2901 1590 125
Fragments arranged by size:
2901 1590 136 125
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 880 4752
Size: 880 3872
AlwNI CAG_nnn'CTG
Cuts at: 0 426 564 978 1106 1194 1271 2004 4275
Size: 426 138 414 128 88 77 733 2271
Cuts at: 4275 4494 4752
Size: 219 258
Fragments arranged by size:
2271 733 426 414 258 219 138 128
88 77
ApaI G_GGCC'C
Cuts at: 0 28 113 119 493 1345 1655 1684 2206
Size: 28 85 6 374 852 310 29 522
Cuts at: 2206 2708 3893 4008 4208 4364 4752
Size: 502 1185 115 200 156 388
Fragments arranged by size:
1185 852 522 502 388 374 310 200
156 115 85 29 28 6
ApaLI G'TGCA_C
Cuts at: 0 3030 3547 4752
Size: 3030 517 1205
Fragments arranged by size:
3030 1205 517
ApoI r'AATT_y
Cuts at: 0 2544 4752
Size: 2544 2208
AvaI C'yCGr_G
Cuts at: 0 21 112 118 322 473 1532 1723 1937
Size: 21 91 6 204 151 1059 191 214
Cuts at: 1937 2701 3775 4150 4210 4324 4752
Size: 764 1074 375 60 114 428
Fragments arranged by size:
1074 1059 764 428 375 214 204 191
151 114 91 60 21 6
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 2657 2690 4752
Size: 2657 33 2062
Fragments arranged by size:
2657 2062 33
BamHI G'GATC_C
Cuts at: 0 35 3329 4320 4752
Size: 35 3294 991 432
Fragments arranged by size:
3294 991 432 35
BanI G'GyrC_C
Cuts at: 0 264 437 775 1228 1708 2397 2482 2632
Size: 264 173 338 453 480 689 85 150
Cuts at: 2632 2733 3939 4215 4302 4472 4699 4752
Size: 101 1206 276 87 170 227 53
Fragments arranged by size:
1206 689 480 453 338 276 264 227
173 170 150 101 87 85 53
BanII G_rGCy'C
Cuts at: 0 28 113 119 467 493 1345 1655 1684
Size: 28 85 6 348 26 852 310 29
Cuts at: 1684 1730 1883 2206 2708 3179 3415 3637 3893
Size: 46 153 323 502 471 236 222 256
Cuts at: 3893 4008 4088 4208 4364 4405 4752
Size: 115 80 120 156 41 347
Fragments arranged by size:
852 502 471 348 347 323 310 256
236 222 156 153 120 115 85 80
46 41 29 28 26 6
BbeI G_GCGC'C
Cuts at: 0 268 441 779 3943 4703 4752
Size: 268 173 338 3164 760 49
Fragments arranged by size:
3164 760 338 268 173 49
BbsI GAAGACnn'nnnn_
Cuts at: 0 2424 3504 4752
Size: 2424 1080 1248
Fragments arranged by size:
2424 1248 1080
BbvCI CC'TCA_GC
Cuts at: 0 4138 4752
Size: 4138 614
BciVI GTATCCnnnnn_n'
Cuts at: 0 2662 3565 4752
Size: 2662 903 1187
Fragments arranged by size:
2662 1187 903
BfrBI ATG'CAT
Cuts at: 0 2284 4752
Size: 2284 2468
BglI GCCn_nnn'nGGC
Cuts at: 0 650 2197 4153 4752
Size: 650 1547 1956 599
Fragments arranged by size:
1956 1547 650 599
BglII A'GATC_T
Cuts at: 0 1736 2012 4752
Size: 1736 276 2740
Fragments arranged by size:
2740 1736 276
BlpI GC'TnA_GC
Cuts at: 0 1958 2803 3997 4752
Size: 1958 845 1194 755
Fragments arranged by size:
1958 1194 845 755
Bme1580I G_kGCm'C
Cuts at: 0 28 113 119 275 493 773 1219 1345
Size: 28 85 6 156 218 280 446 126
Cuts at: 1345 1384 1558 1655 1684 2206 2485 2708 2738
Size: 39 174 97 29 522 279 223 30
Cuts at: 2738 3034 3551 3754 3893 4008 4208 4364 4752
Size: 296 517 203 139 115 200 156 388
Fragments arranged by size:
522 517 446 388 296 280 279 223
218 203 200 174 156 156 139 126
115 97 85 39 30 29 28 6
BmgBI CAC'GTC
Cuts at: 0 3684 4752
Size: 3684 1068
BmrI ACTGGGnnnn_n'
Cuts at: 0 1213 1311 1530 1552 1884 2397 4752
Size: 1213 98 219 22 332 513 2355
Fragments arranged by size:
2355 1213 513 332 219 98 22
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2001 4752
Size: 2001 2751
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 826 928 1083 1248 1272 1446 3280 3544
Size: 826 102 155 165 24 174 1834 264
Cuts at: 3544 4315 4429 4663 4687 4723 4752
Size: 771 114 234 24 36 29
Fragments arranged by size:
1834 826 771 264 234 174 165 155
114 102 36 29 24 24
Bpu10I CC'TnA_GC
Cuts at: 0 2146 4138 4752
Size: 2146 1992 614
Fragments arranged by size:
2146 1992 614
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 726 3910 4752
Size: 726 3184 842
Fragments arranged by size:
3184 842 726
BsaI GGTCTCn'nnnn_
Cuts at: 0 1522 1771 1989 4752
Size: 1522 249 218 2763
Fragments arranged by size:
2763 1522 249 218
BsaAI yAC'GTr
Cuts at: 0 1610 2907 4035 4625 4752
Size: 1610 1297 1128 590 127
Fragments arranged by size:
1610 1297 1128 590 127
BsaHI Gr'CG_yC
Cuts at: 0 75 265 438 776 1585 2249 2744 3940
Size: 75 190 173 338 809 664 495 1196
Cuts at: 3940 4573 4618 4700 4752
Size: 633 45 82 52
Fragments arranged by size:
1196 809 664 633 495 338 190 173
82 75 52 45
BsaWI w'CCGG_w
Cuts at: 0 1054 1805 2090 2750 3131 4499 4601 4752
Size: 1054 751 285 660 381 1368 102 151
Fragments arranged by size:
1368 1054 751 660 381 285 151 102
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 490 520 2596 2626 2761 2791 3466 3496
Size: 490 30 2076 30 135 30 675 30
Cuts at: 3496 4075 4105 4752
Size: 579 30 647
Fragments arranged by size:
2076 675 647 579 490 135 30 30
30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 925 1021 1087 1140 2179 2200 2670 2673
Size: 925 96 66 53 1039 21 470 3
Cuts at: 2673 2967 3625 3793 3796 4096 4752
Size: 294 658 168 3 300 656
Fragments arranged by size:
1039 925 658 656 470 300 294 168
96 66 53 21 3 3
BseYI C'CCAG_C
Cuts at: 0 69 452 541 949 1011 1041 1062 1712
Size: 69 383 89 408 62 30 21 650
Cuts at: 1712 1782 2192 2328 2798 3080 3170 3656 4195
Size: 70 410 136 470 282 90 486 539
Cuts at: 4195 4226 4265 4309 4351 4363 4539 4752
Size: 31 39 44 42 12 176 213
Fragments arranged by size:
650 539 486 470 410 408 383 282
213 176 136 90 89 70 69 62
44 42 39 31 30 21 12
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 160 1188 3037 3167 3841 3847 4752
Size: 160 1028 1849 130 674 6 905
Fragments arranged by size:
1849 1028 905 674 160 130 6
BsiEI CG_ry'CG
Cuts at: 0 92 173 291 2537 3477 3766 4752
Size: 92 81 118 2246 940 289 986
Fragments arranged by size:
2246 986 940 289 118 92 81
BsiHKAI G_wGCw'C
Cuts at: 0 1099 1946 2492 3034 3089 3415 3464 3551
Size: 1099 847 546 542 55 326 49 87
Cuts at: 3551 4405 4752
Size: 854 347
Fragments arranged by size:
1099 854 847 546 542 347 326 87
55 49
BsmI GAATG_Cn'
Cuts at: 0 820 1483 4752
Size: 820 663 3269
Fragments arranged by size:
3269 820 663
BsmBI CGTCTCn'nnnn_
Cuts at: 0 2659 3773 4752
Size: 2659 1114 979
Fragments arranged by size:
2659 1114 979
Bsp1286I G_dGCh'C
Cuts at: 0 28 113 119 275 467 493 773 1099
Size: 28 85 6 156 192 26 280 326
Cuts at: 1099 1219 1345 1384 1558 1655 1684 1730 1883
Size: 120 126 39 174 97 29 46 153
Cuts at: 1883 1946 2206 2485 2492 2708 2738 3034 3089
Size: 63 260 279 7 216 30 296 55
Cuts at: 3089 3179 3415 3464 3551 3637 3754 3893 4008
Size: 90 236 49 87 86 117 139 115
Cuts at: 4008 4088 4208 4364 4405 4752
Size: 80 120 156 41 347
Fragments arranged by size:
347 326 296 280 279 260 236 216
192 174 156 156 153 139 126 120
120 117 115 97 90 87 86 85
80 63 55 49 46 41 39 30
29 28 26 7 6
BspEI T'CCGG_A
Cuts at: 0 2090 2750 3131 4601 4752
Size: 2090 660 381 1470 151
Fragments arranged by size:
2090 1470 660 381 151
BspHI T'CATG_A
Cuts at: 0 3662 4752
Size: 3662 1090
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 868 1114 1312 2054 3300 3869 4752
Size: 868 246 198 742 1246 569 883
Fragments arranged by size:
1246 883 868 742 569 246 198
BsrBI CCG'CTC
Cuts at: 0 3390 3482 4413 4752
Size: 3390 92 931 339
Fragments arranged by size:
3390 931 339 92
BsrDI GCAATG_nn'
Cuts at: 0 1370 4752
Size: 1370 3382
BsrFI r'CCGG_y
Cuts at: 0 592 1054 1697 2771 3602 3837 4299 4752
Size: 592 462 643 1074 831 235 462 453
Fragments arranged by size:
1074 831 643 592 462 462 453 235
BssHII G'CGCG_C
Cuts at: 0 80 4752
Size: 80 4672
BssSI C'ACGA_G
Cuts at: 0 3349 4026 4752
Size: 3349 677 726
Fragments arranged by size:
3349 726 677
BstAPI GCAn_nnn'nTGC
Cuts at: 0 426 1166 4222 4275 4752
Size: 426 740 3056 53 477
Fragments arranged by size:
3056 740 477 426 53
BstEII G'GTnAC_C
Cuts at: 0 1057 4752
Size: 1057 3695
BstXI CCAn_nnnn'nTGG
Cuts at: 0 3204 4752
Size: 3204 1548
BstYI r'GATC_y
Cuts at: 0 35 1736 2012 2141 3329 3497 3687 4320
Size: 35 1701 276 129 1188 168 190 633
Cuts at: 4320 4752
Size: 432
Fragments arranged by size:
1701 1188 633 432 276 190 168 129
35
Bsu36I CC'TnA_GG
Cuts at: 0 2068 2275 4752
Size: 2068 207 2477
Fragments arranged by size:
2477 2068 207
BtgI C'CryG_G
Cuts at: 0 167 229 509 671 690 1084 1278 2378
Size: 167 62 280 162 19 394 194 1100
Cuts at: 2378 2438 4046 4052 4611 4752
Size: 60 1608 6 559 141
Fragments arranged by size:
1608 1100 559 394 280 194 167 162
141 62 60 19 6
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1851 3705 4752
Size: 1851 1854 1047
Fragments arranged by size:
1854 1851 1047
BtsI GCAGTG_nn'
Cuts at: 0 1171 1400 1558 1708 3734 4752
Size: 1171 229 158 150 2026 1018
Fragments arranged by size:
2026 1171 1018 229 158 150
ClaI AT'CG_AT
Cuts at: 0 2060 4752
Size: 2060 2692
DraIII CAC_nnn'GTG
Cuts at: 0 1166 1524 4752
Size: 1166 358 3228
Fragments arranged by size:
3228 1166 358
DrdI GACnn_nn'nnGTC
Cuts at: 0 2838 3450 4752
Size: 2838 612 1302
Fragments arranged by size:
2838 1302 612
EaeI y'GGCC_r
Cuts at: 0 89 170 185 257 288 590 1275 1386
Size: 89 81 15 72 31 302 685 111
Cuts at: 1386 1949 3041 3194 3648 3763 3839 4049 4055
Size: 563 1092 153 454 115 76 210 6
Cuts at: 4055 4752
Size: 697
Fragments arranged by size:
1092 697 685 563 454 302 210 153
115 111 89 81 76 72 31 15
6
EagI C'GGCC_G
Cuts at: 0 89 170 288 3763 4752
Size: 89 81 118 3475 989
Fragments arranged by size:
3475 989 118 89 81
EarI CTCTTCn'nnn_
Cuts at: 0 3496 3508 4752
Size: 3496 12 1244
Fragments arranged by size:
3496 1244 12
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1428 2676 3474 4232 4431 4752
Size: 1428 1248 798 758 199 321
Fragments arranged by size:
1428 1248 798 758 321 199
EcoICRI GAG'CTC
Cuts at: 0 3413 4403 4752
Size: 3413 990 349
Fragments arranged by size:
3413 990 349
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 826 928 1083 1248 1272 1446 3280 3403
Size: 826 102 155 165 24 174 1834 123
Cuts at: 3403 3544 3778 3811 4315 4429 4663 4687 4723
Size: 141 234 33 504 114 234 24 36
Cuts at: 4723 4752
Size: 29
Fragments arranged by size:
1834 826 504 234 234 174 165 155
141 123 114 102 36 33 29 24
24
EcoNI CCTnn'n_nnAGG
Cuts at: 0 754 1951 4752
Size: 754 1197 2801
Fragments arranged by size:
2801 1197 754
EcoO109I rG'GnC_Cy
Cuts at: 0 25 109 490 676 1310 1341 1342 1652
Size: 25 84 381 186 634 31 1 310
Cuts at: 1652 1680 1681 2043 2203 2271 2705 3289 3398
Size: 28 1 362 160 68 434 584 109
Cuts at: 3398 3890 4205 4348 4360 4491 4586 4752
Size: 492 315 143 12 131 95 166
Fragments arranged by size:
634 584 492 434 381 362 315 310
186 166 160 143 131 109 95 84
68 31 28 25 12 1 1
EcoRI G'AATT_C
Cuts at: 0 2544 4752
Size: 2544 2208
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 2448 3969 4752
Size: 2448 1521 783
Fragments arranged by size:
2448 1521 783
FspI TGC'GCA
Cuts at: 0 298 2593 4752
Size: 298 2295 2159
Fragments arranged by size:
2295 2159 298
FspAI rTGC'GCAy
Cuts at: 0 298 4752
Size: 298 4454
HaeII r_GCGC'y
Cuts at: 0 237 268 441 779 1433 2810 3156 3442
Size: 237 31 173 338 654 1377 346 286
Cuts at: 3442 3943 4703 4752
Size: 501 760 49
Fragments arranged by size:
1377 760 654 501 346 338 286 237
173 49 31
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 490 522 1063 1095 1228 1260 2035 2067
Size: 490 32 541 32 133 32 775 32
Cuts at: 2067 2449 2481 2662 2694 2761 2793 3466 3498
Size: 382 32 181 32 67 32 673 32
Cuts at: 3498 4752
Size: 1254
Fragments arranged by size:
1254 775 673 541 490 382 181 133
67 32 32 32 32 32 32 32
32
HincII GTy'rAC
Cuts at: 0 3600 4752
Size: 3600 1152
KasI G'GCGC_C
Cuts at: 0 264 437 775 3939 4699 4752
Size: 264 173 338 3164 760 53
Fragments arranged by size:
3164 760 338 264 173 53
MluI A'CGCG_T
Cuts at: 0 581 1471 4752
Size: 581 890 3281
Fragments arranged by size:
3281 890 581
MscI TGG'CCA
Cuts at: 0 259 1951 3196 4752
Size: 259 1692 1245 1556
Fragments arranged by size:
1692 1556 1245 259
MslI CAynn'nnrTG
Cuts at: 0 136 253 1927 2905 3037 3115 3202 3541
Size: 136 117 1674 978 132 78 87 339
Cuts at: 3541 4627 4752
Size: 1086 125
Fragments arranged by size:
1674 1086 978 339 136 132 125 117
87 78
MspA1I CmG'CkG
Cuts at: 0 48 169 231 281 572 673 692 949
Size: 48 121 62 50 291 101 19 257
Cuts at: 949 1172 1191 1445 1716 1786 1898 2196 2336
Size: 223 19 254 271 70 112 298 140
Cuts at: 2336 3298 3432 4199 4519 4650 4665 4713 4752
Size: 962 134 767 320 131 15 48 39
Fragments arranged by size:
962 767 320 298 291 271 257 254
223 140 134 131 121 112 101 70
62 50 48 48 39 19 19 15
NaeI GCC'GGC
Cuts at: 0 3839 4301 4752
Size: 3839 462 451
Fragments arranged by size:
3839 462 451
NarI GG'CG_CC
Cuts at: 0 265 438 776 3940 4700 4752
Size: 265 173 338 3164 760 52
Fragments arranged by size:
3164 760 338 265 173 52
NgoMIV G'CCGG_C
Cuts at: 0 3837 4299 4752
Size: 3837 462 453
Fragments arranged by size:
3837 462 453
NotI GC'GGCC_GC
Cuts at: 0 89 170 4752
Size: 89 81 4582
Fragments arranged by size:
4582 89 81
NsiI A_TGCA'T
Cuts at: 0 2286 4752
Size: 2286 2466
NspI r_CATG'y
Cuts at: 0 2625 4485 4752
Size: 2625 1860 267
Fragments arranged by size:
2625 1860 267
PasI CC'CwG_GG
Cuts at: 0 1452 2116 4589 4752
Size: 1452 664 2473 163
Fragments arranged by size:
2473 1452 664 163
PciI A'CATG_T
Cuts at: 0 2621 4481 4752
Size: 2621 1860 271
Fragments arranged by size:
2621 1860 271
PflMI CCAn_nnn'nTGG
Cuts at: 0 1225 1524 1564 2004 4752
Size: 1225 299 40 440 2748
Fragments arranged by size:
2748 1225 440 299 40
PfoI T'CCnGG_A
Cuts at: 0 205 964 3572 4344 4487 4752
Size: 205 759 2608 772 143 265
Fragments arranged by size:
2608 772 759 265 205 143
PmlI CAC'GTG
Cuts at: 0 1610 4625 4752
Size: 1610 3015 127
Fragments arranged by size:
3015 1610 127
PpuMI rG'GwC_Cy
Cuts at: 0 676 2043 2271 3289 4348 4491 4752
Size: 676 1367 228 1018 1059 143 261
Fragments arranged by size:
1367 1059 1018 676 261 228 143
PshAI GACnn'nnGTC
Cuts at: 0 584 4747 4752
Size: 584 4163 5
Fragments arranged by size:
4163 584 5
PspOMI G'GGCC_C
Cuts at: 0 24 109 115 489 1341 1651 1680 2202
Size: 24 85 6 374 852 310 29 522
Cuts at: 2202 2704 3889 4004 4204 4360 4752
Size: 502 1185 115 200 156 392
Fragments arranged by size:
1185 852 522 502 392 374 310 200
156 115 85 29 24 6
PspXI vC'TCGA_Gb
Cuts at: 0 322 4752
Size: 322 4430
PstI C_TGCA'G
Cuts at: 0 280 410 431 1028 1309 1550 2525 4511
Size: 280 130 21 597 281 241 975 1986
Cuts at: 4511 4655 4752
Size: 144 97
Fragments arranged by size:
1986 975 597 281 280 241 144 130
97 21
PvuII CAG'CTG
Cuts at: 0 1445 2196 4752
Size: 1445 751 2556
Fragments arranged by size:
2556 1445 751
SacI G_AGCT'C
Cuts at: 0 3415 4405 4752
Size: 3415 990 347
Fragments arranged by size:
3415 990 347
SacII CC_GC'GG
Cuts at: 0 170 232 674 693 4752
Size: 170 62 442 19 4059
Fragments arranged by size:
4059 442 170 62 19
SanDI GG'GwC_CC
Cuts at: 0 676 4348 4752
Size: 676 3672 404
Fragments arranged by size:
3672 676 404
SbfI CC_TGCA'GG
Cuts at: 0 410 1309 4752
Size: 410 899 3443
Fragments arranged by size:
3443 899 410
SexAI A'CCwGG_T
Cuts at: 0 518 2288 2303 4752
Size: 518 1770 15 2449
Fragments arranged by size:
2449 1770 518 15
SfcI C'TryA_G
Cuts at: 0 276 406 427 1024 1155 1305 1546 1914
Size: 276 130 21 597 131 150 241 368
Cuts at: 1914 2034 2103 2493 2521 3696 4507 4651 4752
Size: 120 69 390 28 1175 811 144 101
Fragments arranged by size:
1175 811 597 390 368 276 241 150
144 131 130 120 101 69 28 21
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 4153 4752
Size: 4153 599
SfoI GGC'GCC
Cuts at: 0 266 439 777 3941 4701 4752
Size: 266 173 338 3164 760 51
Fragments arranged by size:
3164 760 338 266 173 51
SmaI CCC'GGG
Cuts at: 0 23 114 475 1939 4326 4752
Size: 23 91 361 1464 2387 426
Fragments arranged by size:
2387 1464 426 361 91 23
SmlI C'TyrA_G
Cuts at: 0 322 705 3925 4752
Size: 322 383 3220 827
Fragments arranged by size:
3220 827 383 322
SrfI GCCC'GGGC
Cuts at: 0 114 4752
Size: 114 4638
StuI AGG'CCT
Cuts at: 0 839 937 1579 3097 4752
Size: 839 98 642 1518 1655
Fragments arranged by size:
1655 1518 839 642 98
StyI C'CwwG_G
Cuts at: 0 260 1952 2213 2393 2600 3215 3806 4076
Size: 260 1692 261 180 207 615 591 270
Cuts at: 4076 4752
Size: 676
Fragments arranged by size:
1692 676 615 591 270 261 260 207
180
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1512 1726 3072 3682 4617 4752
Size: 1512 214 1346 610 935 135
Fragments arranged by size:
1512 1346 935 610 214 135
TatI w'GTAC_w
Cuts at: 0 4091 4752
Size: 4091 661
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1555 1587 4752
Size: 1555 32 3165
Fragments arranged by size:
3165 1555 32
Tth111I GACn'n_nGTC
Cuts at: 0 2410 4752
Size: 2410 2342
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 2626 4710 4752
Size: 2626 2084 42
Fragments arranged by size:
2626 2084 42
XhoI C'TCGA_G
Cuts at: 0 322 4752
Size: 322 4430
XmaI C'CCGG_G
Cuts at: 0 21 112 473 1937 4324 4752
Size: 21 91 361 1464 2387 428
Fragments arranged by size:
2387 1464 428 361 91 21
ZraI GAC'GTC
Cuts at: 0 2250 4752
Size: 2250 2502
Enzymes that do cut:
AarI AatII AcuI AfeI AflIII AgeI AhdI AleI
AlfI AlwNI ApaI ApaLI ApoI AvaI BaeI BamHI
BanI BanII BbeI BbsI BbvCI BciVI BfrBI BglI
BglII BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssHII BssSI BstAPI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI FspAI HaeII Hin4I HincII
KasI MluI MscI MslI MspA1I NaeI NarI NgoMIV
NotI NsiI NspI PasI PciI PflMI PfoI PmlI
PpuMI PshAI PspOMI PspXI PstI PvuII SacI SacII
SanDI SbfI SexAI SfcI SfiI SfoI SmaI SmlI
SrfI StuI StyI TaqII TatI TstI Tth111I XcmI
XhoI XmaI ZraI
Enzymes that do not cut:
AccI Acc65I AclI AflII AloI AscI AseI AsiSI
AvrII BcgI BclI BmtI BsaBI BsiWI BsrGI BstBI
BstZ17I CspCI DraI EcoRV FseI HindIII HpaI KpnI
MfeI MmeI NcoI NdeI NheI NruI PacI PmeI
PpiI PsiI PsrI PvuI RsrII SalI SapI ScaI
SgfI SgrAI SnaBI SpeI SphI SspI SwaI XbaI
XmnI