Restriction map for
pFN21AE9444
(Linear) (Six-base) MAPSORT of: KIEE9444.seq Check: 1137 from: 1 to: 4407
KIEE9444.orf
With 182 enzymes: SgfI *
July 3, 2012 19:02 ..
AatII G_ACGT'C
Cuts at: 0 149 4407
Size: 149 4258
AccI GT'mk_AC
Cuts at: 0 425 3002 4001 4407
Size: 425 2577 999 406
Fragments arranged by size:
2577 999 425 406
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1855 1921 3799 4407
Size: 1855 66 1878 608
Fragments arranged by size:
1878 1855 608 66
AfeI AGC'GCT
Cuts at: 0 1885 3829 4407
Size: 1885 1944 578
Fragments arranged by size:
1944 1885 578
AflIII A'CryG_T
Cuts at: 0 2586 4407
Size: 2586 1821
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2624 3736 4407
Size: 2624 1112 671
Fragments arranged by size:
2624 1112 671
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 4319 4351 4407
Size: 4319 32 56
Fragments arranged by size:
4319 56 32
AlwNI CAG_nnn'CTG
Cuts at: 0 307 1646 4407
Size: 307 1339 2761
Fragments arranged by size:
2761 1339 307
ApoI r'AATT_y
Cuts at: 0 191 953 1613 2140 2436 2565 2651 4407
Size: 191 762 660 527 296 129 86 1756
Fragments arranged by size:
1756 762 660 527 296 191 129 86
AseI AT'TA_AT
Cuts at: 0 3290 4407
Size: 3290 1117
AvaI C'yCGr_G
Cuts at: 0 1508 4363 4407
Size: 1508 2855 44
Fragments arranged by size:
2855 1508 44
AvrII C'CTAG_G
Cuts at: 0 4260 4407
Size: 4260 147
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 3626 3659 4407
Size: 3626 33 748
Fragments arranged by size:
3626 748 33
BamHI G'GATC_C
Cuts at: 0 1620 4046 4407
Size: 1620 2426 361
Fragments arranged by size:
2426 1620 361
BanII G_rGCy'C
Cuts at: 0 1351 2714 4407
Size: 1351 1363 1693
Fragments arranged by size:
1693 1363 1351
BbsI GAAGACnn'nnnn_
Cuts at: 0 588 2062 2138 3554 4407
Size: 588 1474 76 1416 853
Fragments arranged by size:
1474 1416 853 588 76
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 3404 3438 4407
Size: 3404 34 969
Fragments arranged by size:
3404 969 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 897 3627 3642 4407
Size: 897 2730 15 765
Fragments arranged by size:
2730 897 765 15
BclI T'GATC_A
Cuts at: 0 1980 2636 4407
Size: 1980 656 1771
Fragments arranged by size:
1980 1771 656
BfrBI ATG'CAT
Cuts at: 0 1957 4407
Size: 1957 2450
BglII A'GATC_T
Cuts at: 0 1635 4104 4407
Size: 1635 2469 303
Fragments arranged by size:
2469 1635 303
Bme1580I G_kGCm'C
Cuts at: 0 69 4407
Size: 69 4338
BmgBI CAC'GTC
Cuts at: 0 1149 4278 4407
Size: 1149 3129 129
Fragments arranged by size:
3129 1149 129
BmrI ACTGGGnnnn_n'
Cuts at: 0 279 4407
Size: 279 4128
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 4147 4407
Size: 4147 260
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1397 2698 2899 3596 3816 4093 4251 4407
Size: 1397 1301 201 697 220 277 158 156
Fragments arranged by size:
1397 1301 697 277 220 201 158 156
Bpu10I CC'TnA_GC
Cuts at: 0 3709 4407
Size: 3709 698
BsaI GGTCTCn'nnnn_
Cuts at: 0 1958 3748 4407
Size: 1958 1790 659
Fragments arranged by size:
1958 1790 659
BsaAI yAC'GTr
Cuts at: 0 2642 2935 4407
Size: 2642 293 1472
Fragments arranged by size:
2642 1472 293
BsaBI GATnn'nnATC
Cuts at: 0 2683 2788 4407
Size: 2683 105 1619
Fragments arranged by size:
2683 1619 105
BsaHI Gr'CG_yC
Cuts at: 0 146 4407
Size: 146 4261
BsaWI w'CCGG_w
Cuts at: 0 1391 4407
Size: 1391 3016
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1386 1395 1416 1425 4249 4279 4407
Size: 1386 9 21 9 2824 30 128
Fragments arranged by size:
2824 1386 128 30 21 9 9
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 3952 4407
Size: 3952 455
BseYI C'CCAG_C
Cuts at: 0 1473 1753 4407
Size: 1473 280 2654
Fragments arranged by size:
2654 1473 280
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 311 376 2908 2957 4407
Size: 311 65 2532 49 1450
Fragments arranged by size:
2532 1450 311 65 49
BsiHKAI G_wGCw'C
Cuts at: 0 2116 4097 4407
Size: 2116 1981 310
Fragments arranged by size:
2116 1981 310
BsmI GAATG_Cn'
Cuts at: 0 1755 1959 2280 4407
Size: 1755 204 321 2127
Fragments arranged by size:
2127 1755 321 204
Bsp1286I G_dGCh'C
Cuts at: 0 69 1351 2116 2714 4097 4407
Size: 69 1282 765 598 1383 310
Fragments arranged by size:
1383 1282 765 598 310 69
BspHI T'CATG_A
Cuts at: 0 26 152 3963 4407
Size: 26 126 3811 444
Fragments arranged by size:
3811 444 126 26
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 292 4407
Size: 292 4115
BsrDI GCAATG_nn'
Cuts at: 0 572 1088 1709 1916 3420 3689 4407
Size: 572 516 621 207 1504 269 718
Fragments arranged by size:
1504 718 621 572 516 269 207
BsrFI r'CCGG_y
Cuts at: 0 4209 4383 4407
Size: 4209 174 24
Fragments arranged by size:
4209 174 24
BsrGI T'GTAC_A
Cuts at: 0 1809 4407
Size: 1809 2598
BssSI C'ACGA_G
Cuts at: 0 3934 4407
Size: 3934 473
BstBI TT'CG_AA
Cuts at: 0 3546 4407
Size: 3546 861
BstEII G'GTnAC_C
Cuts at: 0 3667 4407
Size: 3667 740
BstXI CCAn_nnnn'nTGG
Cuts at: 0 463 3909 4407
Size: 463 3446 498
Fragments arranged by size:
3446 498 463
BstYI r'GATC_y
Cuts at: 0 1620 1635 4046 4104 4407
Size: 1620 15 2411 58 303
Fragments arranged by size:
2411 1620 303 58 15
BstZ17I GTA'TAC
Cuts at: 0 426 4002 4407
Size: 426 3576 405
Fragments arranged by size:
3576 426 405
BtgI C'CryG_G
Cuts at: 0 1781 2160 2267 2706 3672 3735 4407
Size: 1781 379 107 439 966 63 672
Fragments arranged by size:
1781 966 672 439 379 107 63
BtsI GCAGTG_nn'
Cuts at: 0 299 3888 4326 4407
Size: 299 3589 438 81
Fragments arranged by size:
3589 438 299 81
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 65 100 1931 1966 4407
Size: 65 35 1831 35 2441
Fragments arranged by size:
2441 1831 65 35 35
DraIII CAC_nnn'GTG
Cuts at: 0 1152 4407
Size: 1152 3255
EaeI y'GGCC_r
Cuts at: 0 4 1994 2205 4163 4211 4407
Size: 4 1990 211 1958 48 196
Fragments arranged by size:
1990 1958 211 196 48 4
EarI CTCTTCn'nnn_
Cuts at: 0 1478 2782 3248 3325 4407
Size: 1478 1304 466 77 1082
Fragments arranged by size:
1478 1304 1082 466 77
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1220 4059 4407
Size: 1220 2839 348
Fragments arranged by size:
2839 1220 348
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1397 1855 1921 2698 2899 3596 3799 3816
Size: 1397 458 66 777 201 697 203 17
Cuts at: 3816 4093 4251 4407
Size: 277 158 156
Fragments arranged by size:
1397 777 697 458 277 203 201 158
156 66 17
EcoNI CCTnn'n_nnAGG
Cuts at: 0 3167 4407
Size: 3167 1240
EcoO109I rG'GnC_Cy
Cuts at: 0 304 1291 2254 4355 4407
Size: 304 987 963 2101 52
Fragments arranged by size:
2101 987 963 304 52
EcoRI G'AATT_C
Cuts at: 0 953 4407
Size: 953 3454
EcoRV GAT'ATC
Cuts at: 0 1278 4407
Size: 1278 3129
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 415 585 944 4407
Size: 415 170 359 3463
Fragments arranged by size:
3463 415 359 170
HaeII r_GCGC'y
Cuts at: 0 1887 3831 4407
Size: 1887 1944 576
Fragments arranged by size:
1944 1887 576
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 246 278 445 477 541 573 710 742
Size: 246 32 167 32 64 32 137 32
Cuts at: 742 907 939 1174 1206 1725 1757 1886 1918
Size: 165 32 235 32 519 32 129 32
Cuts at: 1918 3197 3229 3907 3939 4096 4128 4407
Size: 1279 32 678 32 157 32 279
Fragments arranged by size:
1279 678 519 279 246 235 167 165
157 137 129 64 32 32 32 32
32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 0 1926 2498 4407
Size: 1926 572 1909
Fragments arranged by size:
1926 1909 572
HindIII A'AGCT_T
Cuts at: 0 1867 1946 2300 4407
Size: 1867 79 354 2107
Fragments arranged by size:
2107 1867 354 79
HpaI GTT'AAC
Cuts at: 0 1926 4407
Size: 1926 2481
MfeI C'AATT_G
Cuts at: 0 865 4407
Size: 865 3542
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 476 2628 3066 3818 4407
Size: 476 2152 438 752 589
Fragments arranged by size:
2152 752 589 476 438
MscI TGG'CCA
Cuts at: 0 6 4407
Size: 6 4401
MslI CAynn'nnrTG
Cuts at: 0 1748 2606 2869 2905 4407
Size: 1748 858 263 36 1502
Fragments arranged by size:
1748 1502 858 263 36
MspA1I CmG'CkG
Cuts at: 0 1096 1417 1702 1791 1986 2178 2708 2995
Size: 1096 321 285 89 195 192 530 287
Cuts at: 2995 3698 4287 4407
Size: 703 589 120
Fragments arranged by size:
1096 703 589 530 321 287 285 195
192 120 89
NcoI C'CATG_G
Cuts at: 0 1781 2160 3672 4407
Size: 1781 379 1512 735
Fragments arranged by size:
1781 1512 735 379
NdeI CA'TA_TG
Cuts at: 0 912 4407
Size: 912 3495
NsiI A_TGCA'T
Cuts at: 0 1959 4407
Size: 1959 2448
NspI r_CATG'y
Cuts at: 0 2546 2590 4146 4254 4407
Size: 2546 44 1556 108 153
Fragments arranged by size:
2546 1556 153 108 44
PasI CC'CwG_GG
Cuts at: 0 1112 4407
Size: 1112 3295
PciI A'CATG_T
Cuts at: 0 2586 4407
Size: 2586 1821
PflMI CCAn_nnn'nTGG
Cuts at: 0 1911 3664 4407
Size: 1911 1753 743
Fragments arranged by size:
1911 1753 743
PfoI T'CCnGG_A
Cuts at: 0 135 330 405 2876 3486 3908 4407
Size: 135 195 75 2471 610 422 499
Fragments arranged by size:
2471 610 499 422 195 135 75
PmlI CAC'GTG
Cuts at: 0 2642 4407
Size: 2642 1765
PpuMI rG'GwC_Cy
Cuts at: 0 304 1291 2254 4355 4407
Size: 304 987 963 2101 52
Fragments arranged by size:
2101 987 963 304 52
PshAI GACnn'nnGTC
Cuts at: 0 326 4407
Size: 326 4081
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 888 920 3432 3464 4407
Size: 888 32 2512 32 943
Fragments arranged by size:
2512 943 888 32 32
PstI C_TGCA'G
Cuts at: 0 491 1569 3000 4407
Size: 491 1078 1431 1407
Fragments arranged by size:
1431 1407 1078 491
PvuII CAG'CTG
Cuts at: 0 1096 1417 1702 1791 1986 2178 2995 4287
Size: 1096 321 285 89 195 192 817 1292
Cuts at: 4287 4407
Size: 120
Fragments arranged by size:
1292 1096 817 321 285 195 192 120
89
SacII CC_GC'GG
Cuts at: 0 2709 4407
Size: 2709 1698
SanDI GG'GwC_CC
Cuts at: 0 4355 4407
Size: 4355 52
SapI GCTCTTCn'nnn_
Cuts at: 0 2782 3248 4407
Size: 2782 466 1159
Fragments arranged by size:
2782 1159 466
SbfI CC_TGCA'GG
Cuts at: 0 1569 4407
Size: 1569 2838
ScaI AGT'ACT
Cuts at: 0 1398 2576 4407
Size: 1398 1178 1831
Fragments arranged by size:
1831 1398 1178
SfcI C'TryA_G
Cuts at: 0 119 487 528 1565 1574 2996 3006 3340
Size: 119 368 41 1037 9 1422 10 334
Cuts at: 3340 3376 3426 4407
Size: 36 50 981
Fragments arranged by size:
1422 1037 981 368 334 119 50 41
36 10 9
SpeI A'CTAG_T
Cuts at: 0 1399 4399 4407
Size: 1399 3000 8
Fragments arranged by size:
3000 1399 8
SphI G_CATG'C
Cuts at: 0 4146 4254 4407
Size: 4146 108 153
Fragments arranged by size:
4146 153 108
SspI AAT'ATT
Cuts at: 0 1159 2088 4407
Size: 1159 929 2319
Fragments arranged by size:
2319 1159 929
StuI AGG'CCT
Cuts at: 0 1799 4407
Size: 1799 2608
StyI C'CwwG_G
Cuts at: 0 560 1626 1657 1781 2160 3114 3672 3876
Size: 560 1066 31 124 379 954 558 204
Cuts at: 3876 4260 4407
Size: 384 147
Fragments arranged by size:
1066 954 560 558 384 379 204 147
124 31
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 4207 4213 4407
Size: 4207 6 194
Fragments arranged by size:
4207 194 6
TatI w'GTAC_w
Cuts at: 0 1085 1396 1809 2574 3439 4407
Size: 1085 311 413 765 865 968
Fragments arranged by size:
1085 968 865 765 413 311
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 620 652 699 731 1434 1466 4407
Size: 620 32 47 32 703 32 2941
Fragments arranged by size:
2941 703 620 47 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 2065 4407
Size: 2065 2342
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1788 2286 4407
Size: 1788 498 2121
Fragments arranged by size:
2121 1788 498
XmnI GAAnn'nnTTC
Cuts at: 0 3959 4407
Size: 3959 448
ZraI GAC'GTC
Cuts at: 0 147 4407
Size: 147 4260
Enzymes that do cut:
AatII AccI AcuI AfeI AflIII AlfI AloI AlwNI
ApoI AseI AvaI AvrII BaeI BamHI BanII BbsI
BcgI BciVI BclI BfrBI BglII Bme1580I BmgBI BmrI
BplI BpmI Bpu10I BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiHKAI BsmI Bsp1286I BspHI
BspMI BsrDI BsrFI BsrGI BssSI BstBI BstEII BstXI
BstYI BstZ17I BtgI BtsI CspCI DraIII EaeI EarI
EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI HaeII
Hin4I HincII HindIII HpaI MfeI MmeI MscI MslI
MspA1I NcoI NdeI NsiI NspI PasI PciI PflMI
PfoI PmlI PpuMI PshAI PsrI PstI PvuII SacII
SanDI SapI SbfI ScaI SfcI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XcmI XmnI
ZraI
Enzymes that do not cut:
AarI Acc65I AclI AflII AgeI AhdI AleI ApaI
ApaLI AscI AsiSI BanI BbeI BbvCI BglI BlpI
BmtI BpuEI BsiEI BsiWI BsmBI BspEI BsrBI BssHII
BstAPI Bsu36I BtgZI ClaI DraI DrdI EagI EcoICRI
FseI FspI FspAI KasI KpnI MluI NaeI NarI
NgoMIV NheI NotI NruI PacI PmeI PpiI PsiI
PspOMI PspXI PvuI RsrII SacI SalI SexAI SfiI
SfoI SgfI SgrAI SmaI SmlI SnaBI SrfI SwaI
XbaI XhoI XmaI