Restriction map for
pFN21AE9441
(Linear) (Six-base) MAPSORT of: KIEE9441.seq Check: 9733 from: 1 to: 2721
KIEE9441.orf
With 182 enzymes: SgfI *
February 2, 2012 14:09 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1667 2721
Size: 1667 1054
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1111 1925 2414 2721
Size: 1111 814 489 307
Fragments arranged by size:
1111 814 489 307
AflIII A'CryG_T
Cuts at: 0 381 2721
Size: 381 2340
AhdI GACnn_n'nnGTC
Cuts at: 0 1287 2721
Size: 1287 1434
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 154 2721
Size: 154 2567
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1972 2004 2721
Size: 1972 32 717
Fragments arranged by size:
1972 717 32
AlwNI CAG_nnn'CTG
Cuts at: 0 2677 2721
Size: 2677 44
ApoI r'AATT_y
Cuts at: 0 405 742 958 1209 2011 2721
Size: 405 337 216 251 802 710
Fragments arranged by size:
802 710 405 337 251 216
BanI G'GyrC_C
Cuts at: 0 494 940 1882 2119 2721
Size: 494 446 942 237 602
Fragments arranged by size:
942 602 494 446 237
BanII G_rGCy'C
Cuts at: 0 125 170 698 2159 2721
Size: 125 45 528 1461 562
Fragments arranged by size:
1461 562 528 125 45
BbeI G_GCGC'C
Cuts at: 0 2123 2721
Size: 2123 598
BbsI GAAGACnn'nnnn_
Cuts at: 0 189 1598 2721
Size: 189 1409 1123
Fragments arranged by size:
1409 1123 189
BbvCI CC'TCA_GC
Cuts at: 0 91 190 2721
Size: 91 99 2531
Fragments arranged by size:
2531 99 91
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 237 271 2721
Size: 237 34 2450
Fragments arranged by size:
2450 237 34
BclI T'GATC_A
Cuts at: 0 1676 2721
Size: 1676 1045
BfrBI ATG'CAT
Cuts at: 0 1249 2721
Size: 1249 1472
BglI GCCn_nnn'nGGC
Cuts at: 0 1837 2071 2721
Size: 1837 234 650
Fragments arranged by size:
1837 650 234
BglII A'GATC_T
Cuts at: 0 892 1016 1071 2721
Size: 892 124 55 1650
Fragments arranged by size:
1650 892 124 55
BlpI GC'TnA_GC
Cuts at: 0 1192 2721
Size: 1192 1529
Bme1580I G_kGCm'C
Cuts at: 0 461 2067 2721
Size: 461 1606 654
Fragments arranged by size:
1606 654 461
BmrI ACTGGGnnnn_n'
Cuts at: 0 254 1097 1328 2639 2721
Size: 254 843 231 1311 82
Fragments arranged by size:
1311 843 254 231 82
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 295 612 1009 1028 1809 1999 2287 2604
Size: 295 317 397 19 781 190 288 317
Cuts at: 2604 2721
Size: 117
Fragments arranged by size:
781 397 317 317 295 288 190 117
19
Bpu10I CC'TnA_GC
Cuts at: 0 91 142 190 2721
Size: 91 51 48 2531
Fragments arranged by size:
2531 91 51 48
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1387 1532 2579 2721
Size: 1387 145 1047 142
Fragments arranged by size:
1387 1047 145 142
BsaI GGTCTCn'nnnn_
Cuts at: 0 2255 2721
Size: 2255 466
BsaAI yAC'GTr
Cuts at: 0 384 2721
Size: 384 2337
BsaHI Gr'CG_yC
Cuts at: 0 2120 2721
Size: 2120 601
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1043 1073 1972 2002 2010 2040 2721
Size: 1043 30 899 30 8 30 681
Fragments arranged by size:
1043 899 681 30 30 30 8
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 292 2352 2721
Size: 292 2060 369
Fragments arranged by size:
2060 369 292
BseYI C'CCAG_C
Cuts at: 0 2100 2158 2721
Size: 2100 58 563
Fragments arranged by size:
2100 563 58
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1636 2721
Size: 1636 1085
BsiEI CG_ry'CG
Cuts at: 0 59 1204 2721
Size: 59 1145 1517
Fragments arranged by size:
1517 1145 59
BsiHKAI G_wGCw'C
Cuts at: 0 26 1014 2721
Size: 26 988 1707
Fragments arranged by size:
1707 988 26
BsmI GAATG_Cn'
Cuts at: 0 325 483 1464 2721
Size: 325 158 981 1257
Fragments arranged by size:
1257 981 325 158
Bsp1286I G_dGCh'C
Cuts at: 0 26 125 170 461 698 1014 2067 2159
Size: 26 99 45 291 237 316 1053 92
Cuts at: 2159 2721
Size: 562
Fragments arranged by size:
1053 562 316 291 237 99 92 45
26
BspHI T'CATG_A
Cuts at: 0 2213 2721
Size: 2213 508
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1667 2645 2721
Size: 1667 978 76
Fragments arranged by size:
1667 978 76
BsrDI GCAATG_nn'
Cuts at: 0 114 559 2222 2300 2721
Size: 114 445 1663 78 421
Fragments arranged by size:
1663 445 421 114 78
BsrGI T'GTAC_A
Cuts at: 0 1760 2721
Size: 1760 961
BstAPI GCAn_nnn'nTGC
Cuts at: 0 408 2721
Size: 408 2313
BstEII G'GTnAC_C
Cuts at: 0 2604 2721
Size: 2604 117
BstXI CCAn_nnnn'nTGG
Cuts at: 0 576 886 1918 2166 2721
Size: 576 310 1032 248 555
Fragments arranged by size:
1032 576 555 310 248
BstYI r'GATC_y
Cuts at: 0 892 1016 1071 1750 2487 2721
Size: 892 124 55 679 737 234
Fragments arranged by size:
892 737 679 234 124 55
Bsu36I CC'TnA_GG
Cuts at: 0 490 2721
Size: 490 2231
BtgI C'CryG_G
Cuts at: 0 2081 2165 2721
Size: 2081 84 556
Fragments arranged by size:
2081 556 84
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1779 2721
Size: 1779 942
BtsI GCAGTG_nn'
Cuts at: 0 749 1624 1808 2721
Size: 749 875 184 913
Fragments arranged by size:
913 875 749 184
DraI TTT'AAA
Cuts at: 0 722 2721
Size: 722 1999
EaeI y'GGCC_r
Cuts at: 0 2084 2721
Size: 2084 637
EarI CTCTTCn'nnn_
Cuts at: 0 1031 2320 2721
Size: 1031 1289 401
Fragments arranged by size:
1289 1031 401
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 295 612 1009 1028 1111 1809 1925 1999
Size: 295 317 397 19 83 698 116 74
Cuts at: 1999 2287 2414 2604 2721
Size: 288 127 190 117
Fragments arranged by size:
698 397 317 295 288 190 127 117
116 83 74 19
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1631 2721
Size: 1631 1090
EcoO109I rG'GnC_Cy
Cuts at: 0 1636 2721
Size: 1636 1085
EcoRI G'AATT_C
Cuts at: 0 742 2721
Size: 742 1979
EcoRV GAT'ATC
Cuts at: 0 1379 2721
Size: 1379 1342
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 702 2721
Size: 702 2019
HaeII r_GCGC'y
Cuts at: 0 2123 2721
Size: 2123 598
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1001 1008 1033 1040 1273 1305 1461 1493
Size: 1001 7 25 7 233 32 156 32
Cuts at: 1493 2673 2705 2721
Size: 1180 32 16
Fragments arranged by size:
1180 1001 233 156 32 32 32 25
16 7 7
HincII GTy'rAC
Cuts at: 0 521 1173 2721
Size: 521 652 1548
Fragments arranged by size:
1548 652 521
HindIII A'AGCT_T
Cuts at: 0 1123 2541 2721
Size: 1123 1418 180
Fragments arranged by size:
1418 1123 180
HpaI GTT'AAC
Cuts at: 0 521 2721
Size: 521 2200
KasI G'GCGC_C
Cuts at: 0 2119 2721
Size: 2119 602
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 182 209 794 1316 2721
Size: 182 27 585 522 1405
Fragments arranged by size:
1405 585 522 182 27
MslI CAynn'nnrTG
Cuts at: 0 863 1244 2721
Size: 863 381 1477
Fragments arranged by size:
1477 863 381
MspA1I CmG'CkG
Cuts at: 0 51 240 2721
Size: 51 189 2481
Fragments arranged by size:
2481 189 51
NarI GG'CG_CC
Cuts at: 0 2120 2721
Size: 2120 601
NcoI C'CATG_G
Cuts at: 0 2081 2165 2721
Size: 2081 84 556
Fragments arranged by size:
2081 556 84
NsiI A_TGCA'T
Cuts at: 0 1251 2721
Size: 1251 1470
NspI r_CATG'y
Cuts at: 0 1812 2441 2721
Size: 1812 629 280
Fragments arranged by size:
1812 629 280
PasI CC'CwG_GG
Cuts at: 0 672 2721
Size: 672 2049
PflMI CCAn_nnn'nTGG
Cuts at: 0 672 2721
Size: 672 2049
PfoI T'CCnGG_A
Cuts at: 0 272 2721
Size: 272 2449
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 915 947 1972 2004 2721
Size: 915 32 1025 32 717
Fragments arranged by size:
1025 915 717 32 32
PstI C_TGCA'G
Cuts at: 0 50 365 404 2721
Size: 50 315 39 2317
Fragments arranged by size:
2317 315 50 39
PvuI CG_AT'CG
Cuts at: 0 1204 2721
Size: 1204 1517
PvuII CAG'CTG
Cuts at: 0 51 240 2721
Size: 51 189 2481
Fragments arranged by size:
2481 189 51
SapI GCTCTTCn'nnn_
Cuts at: 0 2320 2721
Size: 2320 401
SfcI C'TryA_G
Cuts at: 0 46 361 400 2721
Size: 46 315 39 2321
Fragments arranged by size:
2321 315 46 39
SfoI GGC'GCC
Cuts at: 0 2121 2721
Size: 2121 600
SmlI C'TyrA_G
Cuts at: 0 1402 1547 2594 2721
Size: 1402 145 1047 127
Fragments arranged by size:
1402 1047 145 127
SspI AAT'ATT
Cuts at: 0 2002 2274 2721
Size: 2002 272 447
Fragments arranged by size:
2002 447 272
StyI C'CwwG_G
Cuts at: 0 317 575 2081 2165 2600 2721
Size: 317 258 1506 84 435 121
Fragments arranged by size:
1506 435 317 258 121 84
SwaI ATTT'AAAT
Cuts at: 0 722 2721
Size: 722 1999
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 2092 2552 2721
Size: 2092 460 169
Fragments arranged by size:
2092 460 169
TatI w'GTAC_w
Cuts at: 0 1760 2721
Size: 1760 961
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1476 1508 2721
Size: 1476 32 1213
Fragments arranged by size:
1476 1213 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1704 1833 2172 2721
Size: 1704 129 339 549
Fragments arranged by size:
1704 549 339 129
XmnI GAAnn'nnTTC
Cuts at: 0 1391 2721
Size: 1391 1330
Enzymes that do cut:
AarI AcuI AflIII AhdI AlfI AloI AlwNI ApoI
BanI BanII BbeI BbsI BbvCI BcgI BclI BfrBI
BglI BglII BlpI Bme1580I BmrI BpmI Bpu10I BpuEI
BsaI BsaAI BsaHI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspHI BspMI BsrDI BsrGI BstAPI
BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI DraI
EaeI EarI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI
HaeII Hin4I HincII HindIII HpaI KasI MmeI MslI
MspA1I NarI NcoI NsiI NspI PasI PflMI PfoI
PpiI PstI PvuI PvuII SapI SfcI SfoI SmlI
SspI StyI SwaI TaqII TatI TstI XcmI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AgeI AleI
ApaI ApaLI AscI AseI AsiSI AvaI AvrII BaeI
BamHI BciVI BmgBI BmtI BplI BsaBI BsaWI BsiWI
BsmBI BspEI BsrBI BsrFI BssHII BssSI BstBI BstZ17I
ClaI CspCI DraIII DrdI EagI EciI EcoICRI FseI
FspI FspAI KpnI MfeI MluI MscI NaeI NdeI
NgoMIV NheI NotI NruI PacI PciI PmeI PmlI
PpuMI PshAI PsiI PspOMI PspXI PsrI RsrII SacI
SacII SalI SanDI SbfI ScaI SexAI SfiI SgfI
SgrAI SmaI SnaBI SpeI SphI SrfI StuI Tth111I
XbaI XhoI XmaI ZraI