Restriction map for
pFN21AE9440
(Linear) (Six-base) MAPSORT of: KIEE9440.seq Check: 980 from: 1 to: 1035
KIEE9440.orf
With 182 enzymes: SgfI *
December 27, 2011 16:40 ..
AccI GT'mk_AC
Cuts at: 0 807 1035
Size: 807 228
AleI CACnn'nnGTG
Cuts at: 0 981 1035
Size: 981 54
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 430 1035
Size: 430 605
AvaI C'yCGr_G
Cuts at: 0 896 1035
Size: 896 139
BanII G_rGCy'C
Cuts at: 0 218 251 785 887 897 1035
Size: 218 33 534 102 10 138
Fragments arranged by size:
534 218 138 102 33 10
BbvCI CC'TCA_GC
Cuts at: 0 914 1035
Size: 914 121
Bme1580I G_kGCm'C
Cuts at: 0 820 1035
Size: 820 215
BmrI ACTGGGnnnn_n'
Cuts at: 0 699 1035
Size: 699 336
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 47 97 873 1035
Size: 47 50 776 162
Fragments arranged by size:
776 162 50 47
Bpu10I CC'TnA_GC
Cuts at: 0 779 914 1035
Size: 779 135 121
Fragments arranged by size:
779 135 121
BsaHI Gr'CG_yC
Cuts at: 0 50 1035
Size: 50 985
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 181 931 1035
Size: 181 750 104
Fragments arranged by size:
750 181 104
BseYI C'CCAG_C
Cuts at: 0 185 1013 1035
Size: 185 828 22
Fragments arranged by size:
828 185 22
BsiEI CG_ry'CG
Cuts at: 0 722 1035
Size: 722 313
BsiHKAI G_wGCw'C
Cuts at: 0 251 632 785 887 1035
Size: 251 381 153 102 148
Fragments arranged by size:
381 251 153 148 102
BsiWI C'GTAC_G
Cuts at: 0 270 1035
Size: 270 765
BsmI GAATG_Cn'
Cuts at: 0 788 1035
Size: 788 247
BsmBI CGTCTCn'nnnn_
Cuts at: 0 564 1035
Size: 564 471
Bsp1286I G_dGCh'C
Cuts at: 0 218 251 632 785 820 887 897 1035
Size: 218 33 381 153 35 67 10 138
Fragments arranged by size:
381 218 153 138 67 35 33 10
BspHI T'CATG_A
Cuts at: 0 452 1035
Size: 452 583
BsrDI GCAATG_nn'
Cuts at: 0 392 728 837 1035
Size: 392 336 109 198
Fragments arranged by size:
392 336 198 109
BsrGI T'GTAC_A
Cuts at: 0 2 767 1035
Size: 2 765 268
Fragments arranged by size:
765 268 2
BstAPI GCAn_nnn'nTGC
Cuts at: 0 836 1035
Size: 836 199
BstEII G'GTnAC_C
Cuts at: 0 738 1035
Size: 738 297
BstYI r'GATC_y
Cuts at: 0 149 659 1035
Size: 149 510 376
Fragments arranged by size:
510 376 149
BtgI C'CryG_G
Cuts at: 0 61 1035
Size: 61 974
DrdI GACnn_nn'nnGTC
Cuts at: 0 718 721 1035
Size: 718 3 314
Fragments arranged by size:
718 314 3
EaeI y'GGCC_r
Cuts at: 0 86 1035
Size: 86 949
EarI CTCTTCn'nnn_
Cuts at: 0 139 199 223 538 637 1035
Size: 139 60 24 315 99 398
Fragments arranged by size:
398 315 139 99 60 24
EcoICRI GAG'CTC
Cuts at: 0 249 783 885 1035
Size: 249 534 102 150
Fragments arranged by size:
534 249 150 102
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 47 97 873 1035
Size: 47 50 776 162
Fragments arranged by size:
776 162 50 47
EcoNI CCTnn'n_nnAGG
Cuts at: 0 824 1035
Size: 824 211
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 954 986 1035
Size: 954 32 49
Fragments arranged by size:
954 49 32
MscI TGG'CCA
Cuts at: 0 88 1035
Size: 88 947
MslI CAynn'nnrTG
Cuts at: 0 649 981 1035
Size: 649 332 54
Fragments arranged by size:
649 332 54
NcoI C'CATG_G
Cuts at: 0 61 1035
Size: 61 974
NdeI CA'TA_TG
Cuts at: 0 939 1035
Size: 939 96
PflMI CCAn_nnn'nTGG
Cuts at: 0 749 1035
Size: 749 286
PfoI T'CCnGG_A
Cuts at: 0 373 1035
Size: 373 662
PstI C_TGCA'G
Cuts at: 0 163 1035
Size: 163 872
SacI G_AGCT'C
Cuts at: 0 251 785 887 1035
Size: 251 534 102 148
Fragments arranged by size:
534 251 148 102
SapI GCTCTTCn'nnn_
Cuts at: 0 223 637 1035
Size: 223 414 398
Fragments arranged by size:
414 398 223
SfcI C'TryA_G
Cuts at: 0 159 795 1035
Size: 159 636 240
Fragments arranged by size:
636 240 159
StyI C'CwwG_G
Cuts at: 0 61 1035
Size: 61 974
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 199 639 1035
Size: 199 440 396
Fragments arranged by size:
440 396 199
TatI w'GTAC_w
Cuts at: 0 2 513 767 1035
Size: 2 511 254 268
Fragments arranged by size:
511 268 254 2
Tth111I GACn'n_nGTC
Cuts at: 0 718 1035
Size: 718 317
Enzymes that do cut:
AccI AleI AlfI AvaI BanII BbvCI Bme1580I BmrI
BpmI Bpu10I BsaHI BseRI BseYI BsiEI BsiHKAI BsiWI
BsmI BsmBI Bsp1286I BspHI BsrDI BsrGI BstAPI BstEII
BstYI BtgI DrdI EaeI EarI EcoICRI Eco57MI EcoNI
Hin4I MscI MslI NcoI NdeI PflMI PfoI PstI
SacI SapI SfcI StyI TaqII TatI Tth111I
Enzymes that do not cut:
AarI AatII Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AloI AlwNI ApaI ApaLI ApoI AscI
AseI AsiSI AvrII BaeI BamHI BanI BbeI BbsI
BcgI BciVI BclI BfrBI BglI BglII BlpI BmgBI
BmtI BplI BpuEI BsaI BsaAI BsaBI BsaWI BsaXI
BsgI BspEI BspMI BsrBI BsrFI BssHII BssSI BstBI
BstXI BstZ17I Bsu36I BtgZI BtsI ClaI CspCI DraI
DraIII EagI EciI EcoO109I EcoRI EcoRV FalI FseI
FspI FspAI HaeII HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI MspA1I NaeI NarI NgoMIV NheI
NotI NruI NsiI NspI PacI PasI PciI PmeI
PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI
PvuI PvuII RsrII SacII SalI SanDI SbfI ScaI
SexAI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SrfI SspI StuI SwaI TstI XbaI
XcmI XhoI XmaI XmnI ZraI