(Linear) (Six-base) MAPSORT of: KIEE9438.seq  Check: 2665  from: 1  to: 1074



KIEE9438.orf

 With 182 enzymes: SgfI * 

                        December 27, 2011 16:40  ..

AccI GT'mk_AC

Cuts at:      0     376    1074 
   Size:        376     698
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     335     442    1074 
   Size:        335     107     632

  Fragments arranged by size: 

                 632     335     107
 
AflIII A'CryG_T

Cuts at:      0     371    1074 
   Size:        371     703
 
AleI CACnn'nnGTG

Cuts at:      0     625    1069    1074 
   Size:        625     444       5

  Fragments arranged by size: 

                 625     444       5
 
ApoI r'AATT_y

Cuts at:      0     115     815    1074 
   Size:        115     700     259

  Fragments arranged by size: 

                 700     259     115
 
BamHI G'GATC_C

Cuts at:      0     927    1074 
   Size:        927     147
 
BclI T'GATC_A

Cuts at:      0     500    1074 
   Size:        500     574
 
BsaBI GATnn'nnATC

Cuts at:      0     416     664    1074 
   Size:        416     248     410

  Fragments arranged by size: 

                 416     410     248
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      28     831     845    1074 
   Size:         28     803      14     229

  Fragments arranged by size: 

                 803     229      28      14
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     796    1074 
   Size:        796     278
 
BsrFI r'CCGG_y

Cuts at:      0     135    1074 
   Size:        135     939
 
BsrGI T'GTAC_A

Cuts at:      0     455    1074 
   Size:        455     619
 
BstBI TT'CG_AA

Cuts at:      0     288    1074 
   Size:        288     786
 
BstYI r'GATC_y

Cuts at:      0     194     927    1074 
   Size:        194     733     147

  Fragments arranged by size: 

                 733     194     147
 
BstZ17I GTA'TAC

Cuts at:      0     377    1074 
   Size:        377     697
 
BtsI GCAGTG_nn'

Cuts at:      0     194    1018    1074 
   Size:        194     824      56

  Fragments arranged by size: 

                 824     194      56
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     127    1074 
   Size:        127     947
 
EaeI y'GGCC_r

Cuts at:      0      48     647    1074 
   Size:         48     599     427

  Fragments arranged by size: 

                 599     427      48
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     335     442    1074 
   Size:        335     107     632

  Fragments arranged by size: 

                 632     335     107
 
EcoRI G'AATT_C

Cuts at:      0     815    1074 
   Size:        815     259
 
MfeI C'AATT_G

Cuts at:      0     125    1074 
   Size:        125     949
 
MslI CAynn'nnrTG

Cuts at:      0     625    1069    1074 
   Size:        625     444       5

  Fragments arranged by size: 

                 625     444       5
 
MspA1I CmG'CkG

Cuts at:      0     635    1074 
   Size:        635     439
 
NspI r_CATG'y

Cuts at:      0     375    1074 
   Size:        375     699
 
PciI A'CATG_T

Cuts at:      0     371    1074 
   Size:        371     703
 
PstI C_TGCA'G

Cuts at:      0     343    1074 
   Size:        343     731
 
PvuII CAG'CTG

Cuts at:      0     635    1074 
   Size:        635     439
 
SfcI C'TryA_G

Cuts at:      0     339    1074 
   Size:        339     735
 
StyI C'CwwG_G

Cuts at:      0     316     393    1074 
   Size:        316      77     681

  Fragments arranged by size: 

                 681     316      77
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     486    1074 
   Size:        486     588
 
TatI w'GTAC_w

Cuts at:      0     455    1074 
   Size:        455     619
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     854     886    1074 
   Size:        854      32     188

  Fragments arranged by size: 

                 854     188      32
 

 Enzymes that do cut:

     AccI     AcuI   AflIII     AleI     ApoI    BamHI     BclI    BsaBI
     BsgI    BspMI    BsrFI    BsrGI    BstBI    BstYI  BstZ17I     BtsI
     DrdI     EaeI  Eco57MI    EcoRI     MfeI     MslI   MspA1I     NspI
     PciI     PstI    PvuII     SfcI     StyI    TaqII     TatI     TstI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AfeI    AflII     AgeI     AhdI
     AlfI     AloI    AlwNI     ApaI    ApaLI     AscI     AseI    AsiSI
     AvaI    AvrII     BaeI     BanI    BanII     BbeI     BbsI    BbvCI
     BcgI    BciVI    BfrBI     BglI    BglII     BlpI Bme1580I    BmgBI
     BmrI     BmtI     BplI     BpmI   Bpu10I    BpuEI     BsaI    BsaAI
    BsaHI    BsaWI    BsaXI    BseRI    BseYI    BsiEI  BsiHKAI    BsiWI
     BsmI    BsmBI Bsp1286I    BspEI    BspHI    BsrBI    BsrDI   BssHII
    BssSI   BstAPI   BstEII    BstXI   Bsu36I     BtgI    BtgZI     ClaI
    CspCI     DraI   DraIII     EagI     EarI     EciI  EcoICRI    EcoNI
 EcoO109I    EcoRV     FalI     FseI     FspI    FspAI    HaeII    Hin4I
   HincII  HindIII     HpaI     KasI     KpnI     MluI     MmeI     MscI
     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI
     NsiI     PacI     PasI    PflMI     PfoI     PmeI     PmlI     PpiI
    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI     PvuI    RsrII
     SacI    SacII     SalI    SanDI     SapI     SbfI     ScaI    SexAI
     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI
     SphI     SrfI     SspI     StuI     SwaI  Tth111I     XbaI     XcmI
     XhoI     XmaI     XmnI     ZraI