Restriction map for
pFN21AE9431
(Linear) (Six-base) MAPSORT of: KIEE9431.seq Check: 9157 from: 1 to: 1827
KIEE9431.orf
With 182 enzymes: SgfI *
July 3, 2012 19:02 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 594 1827
Size: 594 1233
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 780 1827
Size: 780 1047
AfeI AGC'GCT
Cuts at: 0 463 1024 1827
Size: 463 561 803
Fragments arranged by size:
803 561 463
AflIII A'CryG_T
Cuts at: 0 1202 1827
Size: 1202 625
AhdI GACnn_n'nnGTC
Cuts at: 0 966 1827
Size: 966 861
AleI CACnn'nnGTG
Cuts at: 0 649 1827
Size: 649 1178
AlwNI CAG_nnn'CTG
Cuts at: 0 594 870 1542 1827
Size: 594 276 672 285
Fragments arranged by size:
672 594 285 276
ApaI G_GGCC'C
Cuts at: 0 1298 1466 1562 1827
Size: 1298 168 96 265
Fragments arranged by size:
1298 265 168 96
ApaLI G'TGCA_C
Cuts at: 0 382 1827
Size: 382 1445
AvaI C'yCGr_G
Cuts at: 0 1297 1459 1555 1714 1782 1827
Size: 1297 162 96 159 68 45
Fragments arranged by size:
1297 162 159 96 68 45
BanI G'GyrC_C
Cuts at: 0 97 611 1304 1827
Size: 97 514 693 523
Fragments arranged by size:
693 523 514 97
BanII G_rGCy'C
Cuts at: 0 704 1249 1298 1425 1466 1562 1642 1715
Size: 704 545 49 127 41 96 80 73
Cuts at: 1715 1819 1827
Size: 104 8
Fragments arranged by size:
704 545 127 104 96 80 73 49
41 8
BbeI G_GCGC'C
Cuts at: 0 101 615 1308 1827
Size: 101 514 693 519
Fragments arranged by size:
693 519 514 101
BbsI GAAGACnn'nnnn_
Cuts at: 0 246 741 1827
Size: 246 495 1086
Fragments arranged by size:
1086 495 246
BbvCI CC'TCA_GC
Cuts at: 0 88 819 1827
Size: 88 731 1008
Fragments arranged by size:
1008 731 88
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 237 271 558 592 1827
Size: 237 34 287 34 1235
Fragments arranged by size:
1235 287 237 34 34
BglI GCCn_nnn'nGGC
Cuts at: 0 770 952 1176 1270 1293 1302 1719 1827
Size: 770 182 224 94 23 9 417 108
Fragments arranged by size:
770 417 224 182 108 94 23 9
BglII A'GATC_T
Cuts at: 0 1085 1827
Size: 1085 742
BlpI GC'TnA_GC
Cuts at: 0 358 1827
Size: 358 1469
Bme1580I G_kGCm'C
Cuts at: 0 179 386 644 1298 1466 1562 1827
Size: 179 207 258 654 168 96 265
Fragments arranged by size:
654 265 258 207 179 168 96
BmgBI CAC'GTC
Cuts at: 0 1203 1827
Size: 1203 624
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1117 1827
Size: 1117 710
Bpu10I CC'TnA_GC
Cuts at: 0 88 819 1693 1827
Size: 88 731 874 134
Fragments arranged by size:
874 731 134 88
BsaI GGTCTCn'nnnn_
Cuts at: 0 247 973 1827
Size: 247 726 854
Fragments arranged by size:
854 726 247
BsaHI Gr'CG_yC
Cuts at: 0 98 518 612 815 1305 1502 1615 1827
Size: 98 420 94 203 490 197 113 212
Fragments arranged by size:
490 420 212 203 197 113 98 94
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 672 979 1827
Size: 672 307 848
Fragments arranged by size:
848 672 307
BseYI C'CCAG_C
Cuts at: 0 1112 1241 1631 1812 1827
Size: 1112 129 390 181 15
Fragments arranged by size:
1112 390 181 129 15
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 50 220 552 622 1783 1827
Size: 50 170 332 70 1161 44
Fragments arranged by size:
1161 332 170 70 50 44
BsiEI CG_ry'CG
Cuts at: 0 903 1370 1827
Size: 903 467 457
Fragments arranged by size:
903 467 457
BsiHKAI G_wGCw'C
Cuts at: 0 386 856 1827
Size: 386 470 971
Fragments arranged by size:
971 470 386
Bsp1286I G_dGCh'C
Cuts at: 0 179 386 644 704 856 1249 1298 1425
Size: 179 207 258 60 152 393 49 127
Cuts at: 1425 1466 1562 1642 1715 1819 1827
Size: 41 96 80 73 104 8
Fragments arranged by size:
393 258 207 179 152 127 104 96
80 73 60 49 41 8
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 594 1152 1166 1827
Size: 594 558 14 661
Fragments arranged by size:
661 594 558 14
BsrBI CCG'CTC
Cuts at: 0 12 1302 1364 1475 1827
Size: 12 1290 62 111 352
Fragments arranged by size:
1290 352 111 62 12
BsrDI GCAATG_nn'
Cuts at: 0 369 1827
Size: 369 1458
BsrFI r'CCGG_y
Cuts at: 0 353 1672 1827
Size: 353 1319 155
Fragments arranged by size:
1319 353 155
BssHII G'CGCG_C
Cuts at: 0 1352 1354 1725 1827
Size: 1352 2 371 102
Fragments arranged by size:
1352 371 102 2
BssSI C'ACGA_G
Cuts at: 0 733 1827
Size: 733 1094
BstAPI GCAn_nnn'nTGC
Cuts at: 0 381 1158 1281 1827
Size: 381 777 123 546
Fragments arranged by size:
777 546 381 123
BstXI CCAn_nnnn'nTGG
Cuts at: 0 651 654 1320 1827
Size: 651 3 666 507
Fragments arranged by size:
666 651 507 3
BstYI r'GATC_y
Cuts at: 0 1085 1596 1827
Size: 1085 511 231
Fragments arranged by size:
1085 511 231
BtgI C'CryG_G
Cuts at: 0 194 474 554 725 857 1414 1827
Size: 194 280 80 171 132 557 413
Fragments arranged by size:
557 413 280 194 171 132 80
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 165 561 1827
Size: 165 396 1266
Fragments arranged by size:
1266 396 165
BtsI GCAGTG_nn'
Cuts at: 0 33 38 1123 1827
Size: 33 5 1085 704
Fragments arranged by size:
1085 704 33 5
ClaI AT'CG_AT
Cuts at: 0 689 1827
Size: 689 1138
DraIII CAC_nnn'GTG
Cuts at: 0 1281 1827
Size: 1281 546
EaeI y'GGCC_r
Cuts at: 0 68 149 170 284 551 596 953 1073
Size: 68 81 21 114 267 45 357 120
Cuts at: 1073 1262 1367 1827
Size: 189 105 460
Fragments arranged by size:
460 357 267 189 120 114 105 81
68 45 21
EagI C'GGCC_G
Cuts at: 0 1367 1827
Size: 1367 460
EarI CTCTTCn'nnn_
Cuts at: 0 589 1827
Size: 589 1238
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1080 1467 1827
Size: 1080 387 360
Fragments arranged by size:
1080 387 360
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 780 1117 1827
Size: 780 337 710
Fragments arranged by size:
780 710 337
EcoO109I rG'GnC_Cy
Cuts at: 0 54 867 1104 1164 1257 1539 1559 1608
Size: 54 813 237 60 93 282 20 49
Cuts at: 1608 1827
Size: 219
Fragments arranged by size:
813 282 237 219 93 60 54 49
20
FspI TGC'GCA
Cuts at: 0 1661 1827
Size: 1661 166
HaeII r_GCGC'y
Cuts at: 0 101 219 465 615 681 826 1026 1182
Size: 101 118 246 150 66 145 200 156
Cuts at: 1182 1308 1631 1827
Size: 126 323 196
Fragments arranged by size:
323 246 200 196 156 150 145 126
118 101 66
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 952 984 1827
Size: 952 32 843
Fragments arranged by size:
952 843 32
HincII GTy'rAC
Cuts at: 0 348 1827
Size: 348 1479
KasI G'GCGC_C
Cuts at: 0 97 611 1304 1827
Size: 97 514 693 523
Fragments arranged by size:
693 523 514 97
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 998 1827
Size: 998 829
MscI TGG'CCA
Cuts at: 0 70 286 553 955 1075 1827
Size: 70 216 267 402 120 752
Fragments arranged by size:
752 402 267 216 120 70
MslI CAynn'nnrTG
Cuts at: 0 157 328 391 559 649 652 1193 1827
Size: 157 171 63 168 90 3 541 634
Fragments arranged by size:
634 541 171 168 157 90 63 3
MspA1I CmG'CkG
Cuts at: 0 204 1221 1416 1570 1635 1827
Size: 204 1017 195 154 65 192
Fragments arranged by size:
1017 204 195 192 154 65
NaeI GCC'GGC
Cuts at: 0 1674 1827
Size: 1674 153
NarI GG'CG_CC
Cuts at: 0 98 612 1305 1827
Size: 98 514 693 522
Fragments arranged by size:
693 522 514 98
NcoI C'CATG_G
Cuts at: 0 474 554 1827
Size: 474 80 1273
Fragments arranged by size:
1273 474 80
NgoMIV G'CCGG_C
Cuts at: 0 1672 1827
Size: 1672 155
NspI r_CATG'y
Cuts at: 0 162 327 1827
Size: 162 165 1500
Fragments arranged by size:
1500 165 162
PasI CC'CwG_GG
Cuts at: 0 1642 1827
Size: 1642 185
PflMI CCAn_nnn'nTGG
Cuts at: 0 77 594 1827
Size: 77 517 1233
Fragments arranged by size:
1233 517 77
PfoI T'CCnGG_A
Cuts at: 0 929 1599 1827
Size: 929 670 228
Fragments arranged by size:
929 670 228
PpuMI rG'GwC_Cy
Cuts at: 0 1164 1257 1827
Size: 1164 93 570
Fragments arranged by size:
1164 570 93
PspOMI G'GGCC_C
Cuts at: 0 1294 1462 1558 1827
Size: 1294 168 96 269
Fragments arranged by size:
1294 269 168 96
PstI C_TGCA'G
Cuts at: 0 722 1163 1827
Size: 722 441 664
Fragments arranged by size:
722 664 441
PvuII CAG'CTG
Cuts at: 0 204 1827
Size: 204 1623
SacII CC_GC'GG
Cuts at: 0 1417 1827
Size: 1417 410
SbfI CC_TGCA'GG
Cuts at: 0 1163 1827
Size: 1163 664
SexAI A'CCwGG_T
Cuts at: 0 1010 1277 1827
Size: 1010 267 550
Fragments arranged by size:
1010 550 267
SfcI C'TryA_G
Cuts at: 0 718 1159 1827
Size: 718 441 668
Fragments arranged by size:
718 668 441
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 952 1827
Size: 952 875
SfoI GGC'GCC
Cuts at: 0 99 613 1306 1827
Size: 99 514 693 521
Fragments arranged by size:
693 521 514 99
SmaI CCC'GGG
Cuts at: 0 1461 1557 1716 1827
Size: 1461 96 159 111
Fragments arranged by size:
1461 159 111 96
SrfI GCCC'GGGC
Cuts at: 0 1557 1827
Size: 1557 270
StyI C'CwwG_G
Cuts at: 0 122 474 554 1827
Size: 122 352 80 1273
Fragments arranged by size:
1273 352 122 80
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 169 487 1827
Size: 169 318 1340
Fragments arranged by size:
1340 318 169
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 192 651 1827
Size: 192 459 1176
Fragments arranged by size:
1176 459 192
XmaI C'CCGG_G
Cuts at: 0 1459 1555 1714 1827
Size: 1459 96 159 113
Fragments arranged by size:
1459 159 113 96
Enzymes that do cut:
AarI AcuI AfeI AflIII AhdI AleI AlwNI ApaI
ApaLI AvaI BanI BanII BbeI BbsI BbvCI BcgI
BglI BglII BlpI Bme1580I BmgBI BpmI Bpu10I BsaI
BsaHI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspMI
BsrBI BsrDI BsrFI BssHII BssSI BstAPI BstXI BstYI
BtgI BtgZI BtsI ClaI DraIII EaeI EagI EarI
EciI Eco57MI EcoO109I FspI HaeII Hin4I HincII KasI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NspI PasI PflMI PfoI PpuMI PspOMI PstI PvuII
SacII SbfI SexAI SfcI SfiI SfoI SmaI SrfI
StyI TaqII XcmI XmaI
Enzymes that do not cut:
AatII AccI Acc65I AclI AflII AgeI AlfI AloI
ApoI AscI AseI AsiSI AvrII BaeI BamHI BciVI
BclI BfrBI BmrI BmtI BplI BpuEI BsaAI BsaBI
BsaWI BsaXI BsiWI BsmI BsmBI BspEI BspHI BsrGI
BstBI BstEII BstZ17I Bsu36I CspCI DraI DrdI EcoICRI
EcoNI EcoRI EcoRV FalI FseI FspAI HindIII HpaI
KpnI MfeI MluI NdeI NheI NotI NruI NsiI
PacI PciI PmeI PmlI PpiI PshAI PsiI PspXI
PsrI PvuI RsrII SacI SalI SanDI SapI ScaI
SgfI SgrAI SmlI SnaBI SpeI SphI SspI StuI
SwaI TatI TstI Tth111I XbaI XhoI XmnI ZraI