Restriction map for
pFN21AE9429
(Linear) (Six-base) MAPSORT of: KIEE9429.seq Check: 5366 from: 1 to: 1059
KIEE9429.orf
With 182 enzymes: SgfI *
December 27, 2011 16:39 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 472 1059
Size: 472 587
AccI GT'mk_AC
Cuts at: 0 92 572 1059
Size: 92 480 487
Fragments arranged by size:
487 480 92
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 533 1059
Size: 533 526
AleI CACnn'nnGTG
Cuts at: 0 532 1059
Size: 532 527
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 550 1024 1059
Size: 550 474 35
Fragments arranged by size:
550 474 35
ApaI G_GGCC'C
Cuts at: 0 726 1059
Size: 726 333
ApoI r'AATT_y
Cuts at: 0 322 1059
Size: 322 737
BamHI G'GATC_C
Cuts at: 0 554 1059
Size: 554 505
BanI G'GyrC_C
Cuts at: 0 579 762 1059
Size: 579 183 297
Fragments arranged by size:
579 297 183
BanII G_rGCy'C
Cuts at: 0 726 1059
Size: 726 333
BbeI G_GCGC'C
Cuts at: 0 766 1059
Size: 766 293
BbsI GAAGACnn'nnnn_
Cuts at: 0 177 521 679 1059
Size: 177 344 158 380
Fragments arranged by size:
380 344 177 158
BclI T'GATC_A
Cuts at: 0 143 1059
Size: 143 916
Bme1580I G_kGCm'C
Cuts at: 0 86 584 726 1059
Size: 86 498 142 333
Fragments arranged by size:
498 333 142 86
BmgBI CAC'GTC
Cuts at: 0 543 1059
Size: 543 516
BmtI G_CTAG'C
Cuts at: 0 820 1059
Size: 820 239
Bpu10I CC'TnA_GC
Cuts at: 0 628 1059
Size: 628 431
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 385 883 1059
Size: 385 498 176
Fragments arranged by size:
498 385 176
BsaI GGTCTCn'nnnn_
Cuts at: 0 420 1059
Size: 420 639
BsaHI Gr'CG_yC
Cuts at: 0 763 1059
Size: 763 296
BseYI C'CCAG_C
Cuts at: 0 155 423 1059
Size: 155 268 636
Fragments arranged by size:
636 268 155
BsiHKAI G_wGCw'C
Cuts at: 0 8 1059
Size: 8 1051
BsmBI CGTCTCn'nnnn_
Cuts at: 0 655 1059
Size: 655 404
Bsp1286I G_dGCh'C
Cuts at: 0 8 86 584 726 1059
Size: 8 78 498 142 333
Fragments arranged by size:
498 333 142 78 8
BspHI T'CATG_A
Cuts at: 0 737 1059
Size: 737 322
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 472 1059
Size: 472 587
BsrBI CCG'CTC
Cuts at: 0 399 1059
Size: 399 660
BsrDI GCAATG_nn'
Cuts at: 0 803 1059
Size: 803 256
BsrFI r'CCGG_y
Cuts at: 0 984 1059
Size: 984 75
BsrGI T'GTAC_A
Cuts at: 0 620 930 1059
Size: 620 310 129
Fragments arranged by size:
620 310 129
BstAPI GCAn_nnn'nTGC
Cuts at: 0 208 1059
Size: 208 851
BstXI CCAn_nnnn'nTGG
Cuts at: 0 757 1059
Size: 757 302
BstYI r'GATC_y
Cuts at: 0 554 977 1059
Size: 554 423 82
Fragments arranged by size:
554 423 82
Bsu36I CC'TnA_GG
Cuts at: 0 290 1059
Size: 290 769
BtgI C'CryG_G
Cuts at: 0 86 654 665 881 1059
Size: 86 568 11 216 178
Fragments arranged by size:
568 216 178 86 11
BtsI GCAGTG_nn'
Cuts at: 0 558 996 1059
Size: 558 438 63
Fragments arranged by size:
558 438 63
DrdI GACnn_nn'nnGTC
Cuts at: 0 223 1059
Size: 223 836
EarI CTCTTCn'nnn_
Cuts at: 0 631 1059
Size: 631 428
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 533 1059
Size: 533 526
EcoO109I rG'GnC_Cy
Cuts at: 0 294 723 1059
Size: 294 429 336
Fragments arranged by size:
429 336 294
EcoRI G'AATT_C
Cuts at: 0 322 1059
Size: 322 737
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 924 1059
Size: 924 135
HaeII r_GCGC'y
Cuts at: 0 766 1059
Size: 766 293
HincII GTy'rAC
Cuts at: 0 365 1059
Size: 365 694
HpaI GTT'AAC
Cuts at: 0 365 1059
Size: 365 694
KasI G'GCGC_C
Cuts at: 0 762 1059
Size: 762 297
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 239 824 1059
Size: 239 585 235
Fragments arranged by size:
585 239 235
MslI CAynn'nnrTG
Cuts at: 0 283 313 532 736 755 1006 1059
Size: 283 30 219 204 19 251 53
Fragments arranged by size:
283 251 219 204 53 30 19
MspA1I CmG'CkG
Cuts at: 0 159 779 1059
Size: 159 620 280
Fragments arranged by size:
620 280 159
NaeI GCC'GGC
Cuts at: 0 986 1059
Size: 986 73
NarI GG'CG_CC
Cuts at: 0 763 1059
Size: 763 296
NcoI C'CATG_G
Cuts at: 0 881 1059
Size: 881 178
NdeI CA'TA_TG
Cuts at: 0 346 1059
Size: 346 713
NgoMIV G'CCGG_C
Cuts at: 0 984 1059
Size: 984 75
NheI G'CTAG_C
Cuts at: 0 816 1059
Size: 816 243
NspI r_CATG'y
Cuts at: 0 282 816 1059
Size: 282 534 243
Fragments arranged by size:
534 282 243
PflMI CCAn_nnn'nTGG
Cuts at: 0 162 506 719 1059
Size: 162 344 213 340
Fragments arranged by size:
344 340 213 162
PpuMI rG'GwC_Cy
Cuts at: 0 294 1059
Size: 294 765
PshAI GACnn'nnGTC
Cuts at: 0 177 1059
Size: 177 882
PspOMI G'GGCC_C
Cuts at: 0 722 1059
Size: 722 337
PvuII CAG'CTG
Cuts at: 0 159 1059
Size: 159 900
SapI GCTCTTCn'nnn_
Cuts at: 0 631 1059
Size: 631 428
SexAI A'CCwGG_T
Cuts at: 0 575 1059
Size: 575 484
SfcI C'TryA_G
Cuts at: 0 96 1059
Size: 96 963
SfoI GGC'GCC
Cuts at: 0 764 1059
Size: 764 295
SmlI C'TyrA_G
Cuts at: 0 400 898 1059
Size: 400 498 161
Fragments arranged by size:
498 400 161
StyI C'CwwG_G
Cuts at: 0 881 1059
Size: 881 178
TatI w'GTAC_w
Cuts at: 0 620 930 1059
Size: 620 310 129
Fragments arranged by size:
620 310 129
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 781 813 1059
Size: 781 32 246
Fragments arranged by size:
781 246 32
Enzymes that do cut:
AarI AccI AcuI AleI AlfI ApaI ApoI BamHI
BanI BanII BbeI BbsI BclI Bme1580I BmgBI BmtI
Bpu10I BpuEI BsaI BsaHI BseYI BsiHKAI BsmBI Bsp1286I
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BstAPI BstXI
BstYI Bsu36I BtgI BtsI DrdI EarI Eco57MI EcoO109I
EcoRI FalI HaeII HincII HpaI KasI MmeI MslI
MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NspI
PflMI PpuMI PshAI PspOMI PvuII SapI SexAI SfcI
SfoI SmlI StyI TatI TstI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII AflIII AgeI AhdI
AloI AlwNI ApaLI AscI AseI AsiSI AvaI AvrII
BaeI BbvCI BcgI BciVI BfrBI BglI BglII BlpI
BmrI BplI BpmI BsaAI BsaBI BsaWI BsaXI BseRI
BsgI BsiEI BsiWI BsmI BspEI BssHII BssSI BstBI
BstEII BstZ17I BtgZI ClaI CspCI DraI DraIII EaeI
EagI EciI EcoICRI EcoNI EcoRV FseI FspI FspAI
Hin4I HindIII KpnI MfeI MluI MscI NotI NruI
NsiI PacI PasI PciI PfoI PmeI PmlI PpiI
PsiI PspXI PsrI PstI PvuI RsrII SacI SacII
SalI SanDI SbfI ScaI SfiI SgfI SgrAI SmaI
SnaBI SpeI SphI SrfI SspI StuI SwaI TaqII
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI