Restriction map for
pFN21AE9428
(Linear) (Six-base) MAPSORT of: KIEE9428.seq Check: 4643 from: 1 to: 1197
KIEE9428.orf
With 182 enzymes: SgfI *
December 27, 2011 16:39 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 402 1197
Size: 402 795
AccI GT'mk_AC
Cuts at: 0 143 1197
Size: 143 1054
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1066 1197
Size: 1066 131
AgeI A'CCGG_T
Cuts at: 0 461 1197
Size: 461 736
AleI CACnn'nnGTG
Cuts at: 0 412 1197
Size: 412 785
ApoI r'AATT_y
Cuts at: 0 516 1197
Size: 516 681
AseI AT'TA_AT
Cuts at: 0 590 1197
Size: 590 607
AvaI C'yCGr_G
Cuts at: 0 7 746 1197
Size: 7 739 451
Fragments arranged by size:
739 451 7
BamHI G'GATC_C
Cuts at: 0 1173 1197
Size: 1173 24
BanII G_rGCy'C
Cuts at: 0 691 1197
Size: 691 506
BbsI GAAGACnn'nnnn_
Cuts at: 0 231 330 1197
Size: 231 99 867
Fragments arranged by size:
867 231 99
BciVI GTATCCnnnnn_n'
Cuts at: 0 862 1197
Size: 862 335
BglII A'GATC_T
Cuts at: 0 1006 1197
Size: 1006 191
BlpI GC'TnA_GC
Cuts at: 0 223 325 1197
Size: 223 102 872
Fragments arranged by size:
872 223 102
Bme1580I G_kGCm'C
Cuts at: 0 113 1197
Size: 113 1084
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 428 1082 1197
Size: 428 654 115
Fragments arranged by size:
654 428 115
Bpu10I CC'TnA_GC
Cuts at: 0 660 1197
Size: 660 537
BsaI GGTCTCn'nnnn_
Cuts at: 0 43 1197
Size: 43 1154
BsaAI yAC'GTr
Cuts at: 0 120 183 873 1197
Size: 120 63 690 324
Fragments arranged by size:
690 324 120 63
BsaWI w'CCGG_w
Cuts at: 0 461 1197
Size: 461 736
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 466 496 1197
Size: 466 30 701
Fragments arranged by size:
701 466 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 681 1197
Size: 681 516
BseYI C'CCAG_C
Cuts at: 0 66 855 985 1197
Size: 66 789 130 212
Fragments arranged by size:
789 212 130 66
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 205 1197
Size: 205 992
BsiEI CG_ry'CG
Cuts at: 0 22 107 1197
Size: 22 85 1090
Fragments arranged by size:
1090 85 22
BsmBI CGTCTCn'nnnn_
Cuts at: 0 576 624 1197
Size: 576 48 573
Fragments arranged by size:
576 573 48
Bsp1286I G_dGCh'C
Cuts at: 0 113 691 1197
Size: 113 578 506
Fragments arranged by size:
578 506 113
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 402 1197
Size: 402 795
BsrDI GCAATG_nn'
Cuts at: 0 1064 1197
Size: 1064 133
BsrFI r'CCGG_y
Cuts at: 0 461 1197
Size: 461 736
BstYI r'GATC_y
Cuts at: 0 1006 1173 1197
Size: 1006 167 24
Fragments arranged by size:
1006 167 24
Bsu36I CC'TnA_GG
Cuts at: 0 1073 1197
Size: 1073 124
BtgI C'CryG_G
Cuts at: 0 58 990 1197
Size: 58 932 207
Fragments arranged by size:
932 207 58
EaeI y'GGCC_r
Cuts at: 0 19 104 314 584 1197
Size: 19 85 210 270 613
Fragments arranged by size:
613 270 210 85 19
EagI C'GGCC_G
Cuts at: 0 19 104 1197
Size: 19 85 1093
Fragments arranged by size:
1093 85 19
EarI CTCTTCn'nnn_
Cuts at: 0 172 262 289 493 607 1197
Size: 172 90 27 204 114 590
Fragments arranged by size:
590 204 172 114 90 27
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 428 1066 1082 1197
Size: 428 638 16 115
Fragments arranged by size:
638 428 115 16
EcoO109I rG'GnC_Cy
Cuts at: 0 63 960 1197
Size: 63 897 237
Fragments arranged by size:
897 237 63
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 675 1197
Size: 675 522
HaeII r_GCGC'y
Cuts at: 0 35 1197
Size: 35 1162
HindIII A'AGCT_T
Cuts at: 0 124 1197
Size: 124 1073
MscI TGG'CCA
Cuts at: 0 586 1197
Size: 586 611
MslI CAynn'nnrTG
Cuts at: 0 181 391 412 1197
Size: 181 210 21 785
Fragments arranged by size:
785 210 181 21
MspA1I CmG'CkG
Cuts at: 0 855 1197
Size: 855 342
NcoI C'CATG_G
Cuts at: 0 990 1197
Size: 990 207
PflMI CCAn_nnn'nTGG
Cuts at: 0 1192 1197
Size: 1192 5
PpuMI rG'GwC_Cy
Cuts at: 0 63 960 1197
Size: 63 897 237
Fragments arranged by size:
897 237 63
PstI C_TGCA'G
Cuts at: 0 374 1197
Size: 374 823
PvuII CAG'CTG
Cuts at: 0 855 1197
Size: 855 342
RsrII CG'GwC_CG
Cuts at: 0 275 1197
Size: 275 922
SanDI GG'GwC_CC
Cuts at: 0 63 1197
Size: 63 1134
SapI GCTCTTCn'nnn_
Cuts at: 0 172 607 1197
Size: 172 435 590
Fragments arranged by size:
590 435 172
SfcI C'TryA_G
Cuts at: 0 370 1197
Size: 370 827
SnaBI TAC'GTA
Cuts at: 0 873 1197
Size: 873 324
SspI AAT'ATT
Cuts at: 0 439 1197
Size: 439 758
StyI C'CwwG_G
Cuts at: 0 683 990 1191 1197
Size: 683 307 201 6
Fragments arranged by size:
683 307 201 6
TatI w'GTAC_w
Cuts at: 0 401 1197
Size: 401 796
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 672 975 1197
Size: 672 303 222
Fragments arranged by size:
672 303 222
Enzymes that do cut:
AarI AccI AcuI AgeI AleI ApoI AseI AvaI
BamHI BanII BbsI BciVI BglII BlpI Bme1580I BpmI
Bpu10I BsaI BsaAI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsmBI Bsp1286I BspMI BsrDI BsrFI BstYI Bsu36I
BtgI EaeI EagI EarI Eco57MI EcoO109I FalI HaeII
HindIII MscI MslI MspA1I NcoI PflMI PpuMI PstI
PvuII RsrII SanDI SapI SfcI SnaBI SspI StyI
TatI XcmI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII AflIII AhdI AlfI
AloI AlwNI ApaI ApaLI AscI AsiSI AvrII BaeI
BanI BbeI BbvCI BcgI BclI BfrBI BglI BmgBI
BmrI BmtI BplI BpuEI BsaBI BsaHI BsiHKAI BsiWI
BsmI BspEI BspHI BsrBI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstZ17I BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EciI EcoICRI EcoNI EcoRI EcoRV
FseI FspI FspAI Hin4I HincII HpaI KasI KpnI
MfeI MluI MmeI NaeI NarI NdeI NgoMIV NheI
NotI NruI NsiI NspI PacI PasI PciI PfoI
PmeI PmlI PpiI PshAI PsiI PspOMI PspXI PsrI
PvuI SacI SacII SalI SbfI ScaI SexAI SfiI
SfoI SgfI SgrAI SmaI SmlI SpeI SphI SrfI
StuI SwaI TaqII TstI Tth111I XbaI XhoI XmaI
XmnI ZraI