Restriction map for
pFN21AE9427
(Linear) (Six-base) MAPSORT of: KIEE9427.seq Check: 2905 from: 1 to: 2622
KIEE9427.orf
With 182 enzymes: SgfI *
March 22, 2012 18:28 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1644 2622
Size: 1644 978
AccI GT'mk_AC
Cuts at: 0 77 545 2606 2622
Size: 77 468 2061 16
Fragments arranged by size:
2061 468 77 16
Acc65I G'GTAC_C
Cuts at: 0 2308 2622
Size: 2308 314
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 154 326 462 1527 2622
Size: 154 172 136 1065 1095
Fragments arranged by size:
1095 1065 172 154 136
AfeI AGC'GCT
Cuts at: 0 1801 2622
Size: 1801 821
AflIII A'CryG_T
Cuts at: 0 1474 2366 2622
Size: 1474 892 256
Fragments arranged by size:
1474 892 256
AleI CACnn'nnGTG
Cuts at: 0 1267 1577 2622
Size: 1267 310 1045
Fragments arranged by size:
1267 1045 310
AlwNI CAG_nnn'CTG
Cuts at: 0 275 1428 1851 2397 2622
Size: 275 1153 423 546 225
Fragments arranged by size:
1153 546 423 275 225
ApoI r'AATT_y
Cuts at: 0 1179 2427 2622
Size: 1179 1248 195
Fragments arranged by size:
1248 1179 195
AvaI C'yCGr_G
Cuts at: 0 1873 2622
Size: 1873 749
BamHI G'GATC_C
Cuts at: 0 158 2622
Size: 158 2464
BanI G'GyrC_C
Cuts at: 0 2131 2308 2622
Size: 2131 177 314
Fragments arranged by size:
2131 314 177
BanII G_rGCy'C
Cuts at: 0 702 1129 1438 2622
Size: 702 427 309 1184
Fragments arranged by size:
1184 702 427 309
BbeI G_GCGC'C
Cuts at: 0 2135 2622
Size: 2135 487
BbsI GAAGACnn'nnnn_
Cuts at: 0 340 755 1509 2622
Size: 340 415 754 1113
Fragments arranged by size:
1113 754 415 340
BbvCI CC'TCA_GC
Cuts at: 0 913 2622
Size: 913 1709
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2175 2209 2622
Size: 2175 34 413
Fragments arranged by size:
2175 413 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 168 918 1092 2622
Size: 168 750 174 1530
Fragments arranged by size:
1530 750 174 168
BclI T'GATC_A
Cuts at: 0 1982 2622
Size: 1982 640
BfrBI ATG'CAT
Cuts at: 0 1347 2622
Size: 1347 1275
BglI GCCn_nnn'nGGC
Cuts at: 0 488 2140 2622
Size: 488 1652 482
Fragments arranged by size:
1652 488 482
BlpI GC'TnA_GC
Cuts at: 0 2566 2622
Size: 2566 56
Bme1580I G_kGCm'C
Cuts at: 0 1885 2622
Size: 1885 737
BmrI ACTGGGnnnn_n'
Cuts at: 0 917 2249 2622
Size: 917 1332 373
Fragments arranged by size:
1332 917 373
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1146 1615 2622
Size: 1146 469 1007
Fragments arranged by size:
1146 1007 469
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 66 163 203 396 1278 1414 1687 2622
Size: 66 97 40 193 882 136 273 935
Fragments arranged by size:
935 882 273 193 136 97 66 40
Bpu10I CC'TnA_GC
Cuts at: 0 562 913 2622
Size: 562 351 1709
Fragments arranged by size:
1709 562 351
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1444 2622
Size: 1444 1178
BsaI GGTCTCn'nnnn_
Cuts at: 0 2243 2622
Size: 2243 379
BsaAI yAC'GTr
Cuts at: 0 1477 2622
Size: 1477 1145
BsaBI GATnn'nnATC
Cuts at: 0 2059 2622
Size: 2059 563
BsaHI Gr'CG_yC
Cuts at: 0 74 118 2132 2622
Size: 74 44 2014 490
Fragments arranged by size:
2014 490 74 44
BsaWI w'CCGG_w
Cuts at: 0 708 1549 1790 2622
Size: 708 841 241 832
Fragments arranged by size:
841 832 708 241
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 176 206 256 286 1531 1544 1561 1574
Size: 176 30 50 30 1245 13 17 13
Cuts at: 1574 2622
Size: 1048
Fragments arranged by size:
1245 1048 176 50 30 30 17 13
13
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1119 2622
Size: 1119 1503
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1078 1605 1948 2622
Size: 1078 527 343 674
Fragments arranged by size:
1078 674 527 343
BsiHKAI G_wGCw'C
Cuts at: 0 568 1264 1736 1786 1913 2622
Size: 568 696 472 50 127 709
Fragments arranged by size:
709 696 568 472 127 50
Bsp1286I G_dGCh'C
Cuts at: 0 568 702 1129 1264 1438 1736 1786 1885
Size: 568 134 427 135 174 298 50 99
Cuts at: 1885 1913 2622
Size: 28 709
Fragments arranged by size:
709 568 427 298 174 135 134 99
50 28
BspEI T'CCGG_A
Cuts at: 0 708 1549 1790 2622
Size: 708 841 241 832
Fragments arranged by size:
841 832 708 241
BspHI T'CATG_A
Cuts at: 0 785 2528 2622
Size: 785 1743 94
Fragments arranged by size:
1743 785 94
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 251 1100 1644 2622
Size: 251 849 544 978
Fragments arranged by size:
978 849 544 251
BsrBI CCG'CTC
Cuts at: 0 696 2622
Size: 696 1926
BsrGI T'GTAC_A
Cuts at: 0 440 2622
Size: 440 2182
BssSI C'ACGA_G
Cuts at: 0 593 622 1234 1587 2313 2478 2622
Size: 593 29 612 353 726 165 144
Fragments arranged by size:
726 612 593 353 165 144 29
BstAPI GCAn_nnn'nTGC
Cuts at: 0 2108 2622
Size: 2108 514
BstEII G'GTnAC_C
Cuts at: 0 1113 1755 2622
Size: 1113 642 867
Fragments arranged by size:
1113 867 642
BstXI CCAn_nnnn'nTGG
Cuts at: 0 2283 2622
Size: 2283 339
BstYI r'GATC_y
Cuts at: 0 158 989 1226 2240 2622
Size: 158 831 237 1014 382
Fragments arranged by size:
1014 831 382 237 158
BtgI C'CryG_G
Cuts at: 0 980 1034 1118 1136 1151 1206 1572 2081
Size: 980 54 84 18 15 55 366 509
Cuts at: 2081 2244 2622
Size: 163 378
Fragments arranged by size:
980 509 378 366 163 84 55 54
18 15
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 2178 2622
Size: 2178 444
BtsI GCAGTG_nn'
Cuts at: 0 301 731 2288 2622
Size: 301 430 1557 334
Fragments arranged by size:
1557 430 334 301
DraIII CAC_nnn'GTG
Cuts at: 0 1689 2622
Size: 1689 933
EaeI y'GGCC_r
Cuts at: 0 136 1154 1745 1904 2285 2622
Size: 136 1018 591 159 381 337
Fragments arranged by size:
1018 591 381 337 159 136
EarI CTCTTCn'nnn_
Cuts at: 0 787 848 2054 2622
Size: 787 61 1206 568
Fragments arranged by size:
1206 787 568 61
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1858 2478 2622
Size: 1858 620 144
Fragments arranged by size:
1858 620 144
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 66 154 163 203 326 396 462 1278
Size: 66 88 9 40 123 70 66 816
Cuts at: 1278 1414 1527 1687 2622
Size: 136 113 160 935
Fragments arranged by size:
935 816 160 136 123 113 88 70
66 66 40 9
EcoNI CCTnn'n_nnAGG
Cuts at: 0 226 1245 1651 2622
Size: 226 1019 406 971
Fragments arranged by size:
1019 971 406 226
EcoO109I rG'GnC_Cy
Cuts at: 0 490 534 557 1848 2537 2622
Size: 490 44 23 1291 689 85
Fragments arranged by size:
1291 689 490 85 44 23
EcoRI G'AATT_C
Cuts at: 0 1179 2427 2622
Size: 1179 1248 195
Fragments arranged by size:
1248 1179 195
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 987 2622
Size: 987 1635
HaeII r_GCGC'y
Cuts at: 0 1034 1696 1803 2135 2228 2622
Size: 1034 662 107 332 93 394
Fragments arranged by size:
1034 662 394 332 107 93
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 371 403 1531 1544 1563 1576 2622
Size: 371 32 1128 13 19 13 1046
Fragments arranged by size:
1128 1046 371 32 19 13 13
HincII GTy'rAC
Cuts at: 0 207 552 618 2178 2270 2340 2607 2622
Size: 207 345 66 1560 92 70 267 15
Fragments arranged by size:
1560 345 267 207 92 70 66 15
KasI G'GCGC_C
Cuts at: 0 2131 2622
Size: 2131 491
KpnI G_GTAC'C
Cuts at: 0 2312 2622
Size: 2312 310
MfeI C'AATT_G
Cuts at: 0 2106 2622
Size: 2106 516
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 635 988 1112 1979 2525 2622
Size: 635 353 124 867 546 97
Fragments arranged by size:
867 635 546 353 124 97
MscI TGG'CCA
Cuts at: 0 138 1156 1747 2622
Size: 138 1018 591 875
Fragments arranged by size:
1018 875 591 138
MslI CAynn'nnrTG
Cuts at: 0 1069 1267 1577 1648 1654 2197 2622
Size: 1069 198 310 71 6 543 425
Fragments arranged by size:
1069 543 425 310 198 71 6
MspA1I CmG'CkG
Cuts at: 0 416 1619 1665 2622
Size: 416 1203 46 957
Fragments arranged by size:
1203 957 416 46
NarI GG'CG_CC
Cuts at: 0 2132 2622
Size: 2132 490
NcoI C'CATG_G
Cuts at: 0 1136 2081 2244 2622
Size: 1136 945 163 378
Fragments arranged by size:
1136 945 378 163
NsiI A_TGCA'T
Cuts at: 0 1349 2622
Size: 1349 1273
NspI r_CATG'y
Cuts at: 0 754 1293 2370 2622
Size: 754 539 1077 252
Fragments arranged by size:
1077 754 539 252
PciI A'CATG_T
Cuts at: 0 2366 2622
Size: 2366 256
PflMI CCAn_nnn'nTGG
Cuts at: 0 464 1902 2468 2622
Size: 464 1438 566 154
Fragments arranged by size:
1438 566 464 154
PfoI T'CCnGG_A
Cuts at: 0 99 876 1535 2622
Size: 99 777 659 1087
Fragments arranged by size:
1087 777 659 99
PpuMI rG'GwC_Cy
Cuts at: 0 557 1848 2537 2622
Size: 557 1291 689 85
Fragments arranged by size:
1291 689 557 85
PshAI GACnn'nnGTC
Cuts at: 0 505 2206 2622
Size: 505 1701 416
Fragments arranged by size:
1701 505 416
PspXI vC'TCGA_Gb
Cuts at: 0 1873 2622
Size: 1873 749
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 1786 1818 2622
Size: 1786 32 804
Fragments arranged by size:
1786 804 32
PstI C_TGCA'G
Cuts at: 0 2393 2622
Size: 2393 229
SalI G'TCGA_C
Cuts at: 0 2605 2622
Size: 2605 17
SanDI GG'GwC_CC
Cuts at: 0 557 2537 2622
Size: 557 1980 85
Fragments arranged by size:
1980 557 85
SbfI CC_TGCA'GG
Cuts at: 0 2393 2622
Size: 2393 229
SexAI A'CCwGG_T
Cuts at: 0 1685 2622
Size: 1685 937
SfcI C'TryA_G
Cuts at: 0 1971 2389 2622
Size: 1971 418 233
Fragments arranged by size:
1971 418 233
SfoI GGC'GCC
Cuts at: 0 2133 2622
Size: 2133 489
SmlI C'TyrA_G
Cuts at: 0 1459 1873 2622
Size: 1459 414 749
Fragments arranged by size:
1459 749 414
SphI G_CATG'C
Cuts at: 0 754 1293 2622
Size: 754 539 1329
Fragments arranged by size:
1329 754 539
StuI AGG'CCT
Cuts at: 0 472 1240 2622
Size: 472 768 1382
Fragments arranged by size:
1382 768 472
StyI C'CwwG_G
Cuts at: 0 527 1136 2081 2244 2276 2462 2572 2622
Size: 527 609 945 163 32 186 110 50
Fragments arranged by size:
945 609 527 186 163 110 50 32
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1425 2622
Size: 1425 1197
TatI w'GTAC_w
Cuts at: 0 440 725 1523 2363 2622
Size: 440 285 798 840 259
Fragments arranged by size:
840 798 440 285 259
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 95 127 176 208 558 590 1372 1404
Size: 95 32 49 32 350 32 782 32
Cuts at: 1404 1673 1705 2622
Size: 269 32 917
Fragments arranged by size:
917 782 350 269 95 49 32 32
32 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 1066 2622
Size: 1066 1556
XbaI T'CTAG_A
Cuts at: 0 352 2622
Size: 352 2270
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 494 2622
Size: 494 2128
XhoI C'TCGA_G
Cuts at: 0 1873 2622
Size: 1873 749
XmnI GAAnn'nnTTC
Cuts at: 0 2512 2622
Size: 2512 110
Enzymes that do cut:
AarI AccI Acc65I AcuI AfeI AflIII AleI AlwNI
ApoI AvaI BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BfrBI BglI BlpI Bme1580I BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BsgI BsiHKAI Bsp1286I BspEI BspHI
BspMI BsrBI BsrGI BssSI BstAPI BstEII BstXI BstYI
BtgI BtgZI BtsI DraIII EaeI EarI EciI Eco57MI
EcoNI EcoO109I EcoRI FalI HaeII Hin4I HincII KasI
KpnI MfeI MmeI MscI MslI MspA1I NarI NcoI
NsiI NspI PciI PflMI PfoI PpuMI PshAI PspXI
PsrI PstI SalI SanDI SbfI SexAI SfcI SfoI
SmlI SphI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmnI
Enzymes that do not cut:
AatII AclI AflII AgeI AhdI AlfI AloI ApaI
ApaLI AscI AseI AsiSI AvrII BaeI BglII BmgBI
BmtI BseYI BsiEI BsiWI BsmI BsmBI BsrDI BsrFI
BssHII BstBI BstZ17I Bsu36I ClaI CspCI DraI DrdI
EagI EcoICRI EcoRV FseI FspI FspAI HindIII HpaI
MluI NaeI NdeI NgoMIV NheI NotI NruI PacI
PasI PmeI PmlI PpiI PsiI PspOMI PvuI PvuII
RsrII SacI SacII SapI ScaI SfiI SgfI SgrAI
SmaI SnaBI SpeI SrfI SspI SwaI XmaI ZraI