Restriction map for
pFN21AE9423
(Linear) (Six-base) MAPSORT of: KIEE9423.seq Check: 7403 from: 1 to: 2436
KIEE9423.orf
With 182 enzymes: SgfI *
March 22, 2012 18:27 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 412 1004 2208 2436
Size: 412 592 1204 228
Fragments arranged by size:
1204 592 412 228
AccI GT'mk_AC
Cuts at: 0 1112 2436
Size: 1112 1324
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1047 2436
Size: 1047 1389
AflIII A'CryG_T
Cuts at: 0 344 346 2126 2436
Size: 344 2 1780 310
Fragments arranged by size:
1780 344 310 2
AleI CACnn'nnGTG
Cuts at: 0 487 2436
Size: 487 1949
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1693 2436
Size: 1693 743
AlwNI CAG_nnn'CTG
Cuts at: 0 83 441 861 921 2436
Size: 83 358 420 60 1515
Fragments arranged by size:
1515 420 358 83 60
ApoI r'AATT_y
Cuts at: 0 241 2061 2436
Size: 241 1820 375
Fragments arranged by size:
1820 375 241
AvaI C'yCGr_G
Cuts at: 0 576 1392 2234 2436
Size: 576 816 842 202
Fragments arranged by size:
842 816 576 202
BamHI G'GATC_C
Cuts at: 0 468 1563 2436
Size: 468 1095 873
Fragments arranged by size:
1095 873 468
BanI G'GyrC_C
Cuts at: 0 95 400 1477 2436
Size: 95 305 1077 959
Fragments arranged by size:
1077 959 305 95
BbsI GAAGACnn'nnnn_
Cuts at: 0 1407 2274 2436
Size: 1407 867 162
Fragments arranged by size:
1407 867 162
BbvCI CC'TCA_GC
Cuts at: 0 1211 1348 2308 2436
Size: 1211 137 960 128
Fragments arranged by size:
1211 960 137 128
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 203 237 2436
Size: 203 34 2199
Fragments arranged by size:
2199 203 34
BclI T'GATC_A
Cuts at: 0 1737 2436
Size: 1737 699
BglI GCCn_nnn'nGGC
Cuts at: 0 446 578 725 2436
Size: 446 132 147 1711
Fragments arranged by size:
1711 446 147 132
BlpI GC'TnA_GC
Cuts at: 0 1597 2174 2436
Size: 1597 577 262
Fragments arranged by size:
1597 577 262
Bme1580I G_kGCm'C
Cuts at: 0 90 100 2436
Size: 90 10 2336
Fragments arranged by size:
2336 90 10
BmgBI CAC'GTC
Cuts at: 0 2127 2436
Size: 2127 309
BmrI ACTGGGnnnn_n'
Cuts at: 0 1351 1458 2436
Size: 1351 107 978
Fragments arranged by size:
1351 978 107
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 666 1622 2436
Size: 666 956 814
Fragments arranged by size:
956 814 666
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 60 833 955 973 1114 1843 1944 2376
Size: 60 773 122 18 141 729 101 432
Cuts at: 2376 2436
Size: 60
Fragments arranged by size:
773 729 432 141 122 101 60 60
18
Bpu10I CC'TnA_GC
Cuts at: 0 1030 1211 1348 1516 2308 2402 2436
Size: 1030 181 137 168 792 94 34
Fragments arranged by size:
1030 792 181 168 137 94 34
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1149 1860 2436
Size: 1149 711 576
Fragments arranged by size:
1149 711 576
BsaI GGTCTCn'nnnn_
Cuts at: 0 949 2436
Size: 949 1487
BsaAI yAC'GTr
Cuts at: 0 347 2198 2436
Size: 347 1851 238
Fragments arranged by size:
1851 347 238
BsaWI w'CCGG_w
Cuts at: 0 505 1744 2138 2436
Size: 505 1239 394 298
Fragments arranged by size:
1239 505 394 298
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 26 1336 1366 1816 1846 2092 2122 2374
Size: 26 1310 30 450 30 246 30 252
Cuts at: 2374 2404 2436
Size: 30 32
Fragments arranged by size:
1310 450 252 246 32 30 30 30
30 26
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1573 1609 1708 2100 2296 2436
Size: 1573 36 99 392 196 140
Fragments arranged by size:
1573 392 196 140 99 36
BseYI C'CCAG_C
Cuts at: 0 1929 2422 2436
Size: 1929 493 14
Fragments arranged by size:
1929 493 14
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 306 465 666 1120 1573 1832 2178 2436
Size: 306 159 201 454 453 259 346 258
Fragments arranged by size:
454 453 346 306 259 258 201 159
BsiHKAI G_wGCw'C
Cuts at: 0 1409 1765 2436
Size: 1409 356 671
Fragments arranged by size:
1409 671 356
BsmI GAATG_Cn'
Cuts at: 0 31 502 2436
Size: 31 471 1934
Fragments arranged by size:
1934 471 31
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1608 2436
Size: 1608 828
Bsp1286I G_dGCh'C
Cuts at: 0 90 100 1409 1765 2436
Size: 90 10 1309 356 671
Fragments arranged by size:
1309 671 356 90 10
BspEI T'CCGG_A
Cuts at: 0 505 2138 2436
Size: 505 1633 298
Fragments arranged by size:
1633 505 298
BspHI T'CATG_A
Cuts at: 0 1892 2436
Size: 1892 544
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 412 439 1004 2208 2307 2436
Size: 412 27 565 1204 99 129
Fragments arranged by size:
1204 565 412 129 99 27
BsrFI r'CCGG_y
Cuts at: 0 1774 2436
Size: 1774 662
BssSI C'ACGA_G
Cuts at: 0 111 1726 2436
Size: 111 1615 710
Fragments arranged by size:
1615 710 111
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1499 2436
Size: 1499 937
BstEII G'GTnAC_C
Cuts at: 0 1183 2436
Size: 1183 1253
BstXI CCAn_nnnn'nTGG
Cuts at: 0 183 2436
Size: 183 2253
BstYI r'GATC_y
Cuts at: 0 468 1563 2436
Size: 468 1095 873
Fragments arranged by size:
1095 873 468
Bsu36I CC'TnA_GG
Cuts at: 0 101 464 2436
Size: 101 363 1972
Fragments arranged by size:
1972 363 101
BtgI C'CryG_G
Cuts at: 0 182 695 2436
Size: 182 513 1741
Fragments arranged by size:
1741 513 182
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1755 2436
Size: 1755 681
BtsI GCAGTG_nn'
Cuts at: 0 534 1151 1675 1844 2403 2436
Size: 534 617 524 169 559 33
Fragments arranged by size:
617 559 534 524 169 33
DraI TTT'AAA
Cuts at: 0 2163 2436
Size: 2163 273
DraIII CAC_nnn'GTG
Cuts at: 0 984 1755 2201 2436
Size: 984 771 446 235
Fragments arranged by size:
984 771 446 235
DrdI GACnn_nn'nnGTC
Cuts at: 0 1120 2436
Size: 1120 1316
EaeI y'GGCC_r
Cuts at: 0 887 986 1428 1799 1943 2436
Size: 887 99 442 371 144 493
Fragments arranged by size:
887 493 442 371 144 99
EarI CTCTTCn'nnn_
Cuts at: 0 1708 1726 1843 2257 2436
Size: 1708 18 117 414 179
Fragments arranged by size:
1708 414 179 117 18
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 60 833 955 973 1047 1114 1843 1944
Size: 60 773 122 18 74 67 729 101
Cuts at: 1944 2376 2436
Size: 432 60
Fragments arranged by size:
773 729 432 122 101 74 67 60
60 18
EcoO109I rG'GnC_Cy
Cuts at: 0 599 858 939 948 1215 1827 2436
Size: 599 259 81 9 267 612 609
Fragments arranged by size:
612 609 599 267 259 81 9
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 900 2436
Size: 900 1536
HaeII r_GCGC'y
Cuts at: 0 922 2436
Size: 922 1514
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 28 731 763 1122 1154 1297 1329 2436
Size: 28 703 32 359 32 143 32 1107
Fragments arranged by size:
1107 703 359 143 32 32 32 28
HincII GTy'rAC
Cuts at: 0 411 1869 2433 2436
Size: 411 1458 564 3
Fragments arranged by size:
1458 564 411 3
HpaI GTT'AAC
Cuts at: 0 2433 2436
Size: 2433 3
MfeI C'AATT_G
Cuts at: 0 1733 2436
Size: 1733 703
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 2025 2436
Size: 2025 411
MscI TGG'CCA
Cuts at: 0 889 988 1430 1801 1945 2436
Size: 889 99 442 371 144 491
Fragments arranged by size:
889 491 442 371 144 99
MslI CAynn'nnrTG
Cuts at: 0 181 487 665 1547 1588 1813 1973 2149
Size: 181 306 178 882 41 225 160 176
Cuts at: 2149 2436
Size: 287
Fragments arranged by size:
882 306 287 225 181 178 176 160
41
MspA1I CmG'CkG
Cuts at: 0 569 710 716 1933 1991 2178 2436
Size: 569 141 6 1217 58 187 258
Fragments arranged by size:
1217 569 258 187 141 58 6
NcoI C'CATG_G
Cuts at: 0 182 2436
Size: 182 2254
PasI CC'CwG_GG
Cuts at: 0 943 1189 2436
Size: 943 246 1247
Fragments arranged by size:
1247 943 246
PflMI CCAn_nnn'nTGG
Cuts at: 0 861 2436
Size: 861 1575
PfoI T'CCnGG_A
Cuts at: 0 854 1226 2321 2436
Size: 854 372 1095 115
Fragments arranged by size:
1095 854 372 115
PmlI CAC'GTG
Cuts at: 0 347 2198 2436
Size: 347 1851 238
Fragments arranged by size:
1851 347 238
PpuMI rG'GwC_Cy
Cuts at: 0 599 858 948 2436
Size: 599 259 90 1488
Fragments arranged by size:
1488 599 259 90
PshAI GACnn'nnGTC
Cuts at: 0 365 1864 2436
Size: 365 1499 572
Fragments arranged by size:
1499 572 365
PstI C_TGCA'G
Cuts at: 0 1077 1280 1468 1688 1956 2276 2436
Size: 1077 203 188 220 268 320 160
Fragments arranged by size:
1077 320 268 220 203 188 160
PvuII CAG'CTG
Cuts at: 0 710 1933 2178 2436
Size: 710 1223 245 258
Fragments arranged by size:
1223 710 258 245
SanDI GG'GwC_CC
Cuts at: 0 599 2436
Size: 599 1837
SbfI CC_TGCA'GG
Cuts at: 0 1077 2276 2436
Size: 1077 1199 160
Fragments arranged by size:
1199 1077 160
SfcI C'TryA_G
Cuts at: 0 1073 1276 1464 1684 1952 2272 2436
Size: 1073 203 188 220 268 320 164
Fragments arranged by size:
1073 320 268 220 203 188 164
SmaI CCC'GGG
Cuts at: 0 578 2236 2436
Size: 578 1658 200
Fragments arranged by size:
1658 578 200
SmlI C'TyrA_G
Cuts at: 0 1128 1875 2436
Size: 1128 747 561
Fragments arranged by size:
1128 747 561
SrfI GCCC'GGGC
Cuts at: 0 578 2436
Size: 578 1858
StuI AGG'CCT
Cuts at: 0 1028 1965 2436
Size: 1028 937 471
Fragments arranged by size:
1028 937 471
StyI C'CwwG_G
Cuts at: 0 182 379 417 2031 2436
Size: 182 197 38 1614 405
Fragments arranged by size:
1614 405 197 182 38
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 547 1354 1525 2436
Size: 547 807 171 911
Fragments arranged by size:
911 807 547 171
TatI w'GTAC_w
Cuts at: 0 2297 2436
Size: 2297 139
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 97 129 1739 1771 2374 2406 2436
Size: 97 32 1610 32 603 32 30
Fragments arranged by size:
1610 603 97 32 32 32 30
Tth111I GACn'n_nGTC
Cuts at: 0 769 850 2436
Size: 769 81 1586
Fragments arranged by size:
1586 769 81
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 442 996 1438 2436
Size: 442 554 442 998
Fragments arranged by size:
998 554 442 442
XmaI C'CCGG_G
Cuts at: 0 576 2234 2436
Size: 576 1658 202
Fragments arranged by size:
1658 576 202
XmnI GAAnn'nnTTC
Cuts at: 0 1983 2436
Size: 1983 453
Enzymes that do cut:
AarI AccI AcuI AflIII AleI AlfI AlwNI ApoI
AvaI BamHI BanI BbsI BbvCI BcgI BclI BglI
BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrFI BssSI
BstAPI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI
DraI DraIII DrdI EaeI EarI Eco57MI EcoO109I FalI
HaeII Hin4I HincII HpaI MfeI MmeI MscI MslI
MspA1I NcoI PasI PflMI PfoI PmlI PpuMI PshAI
PstI PvuII SanDI SbfI SfcI SmaI SmlI SrfI
StuI StyI TaqII TatI TstI Tth111I XcmI XmaI
XmnI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII AgeI AhdI AloI
ApaI ApaLI AscI AseI AsiSI AvrII BaeI BanII
BbeI BciVI BfrBI BglII BmtI BsaBI BsaHI BsiEI
BsiWI BsrBI BsrDI BsrGI BssHII BstBI BstZ17I ClaI
CspCI EagI EciI EcoICRI EcoNI EcoRI EcoRV FseI
FspI FspAI HindIII KasI KpnI MluI NaeI NarI
NdeI NgoMIV NheI NotI NruI NsiI NspI PacI
PciI PmeI PpiI PsiI PspOMI PspXI PsrI PvuI
RsrII SacI SacII SalI SapI ScaI SexAI SfiI
SfoI SgfI SgrAI SnaBI SpeI SphI SspI SwaI
XbaI XhoI ZraI