(Linear) (Six-base) MAPSORT of: KIEE9421.seq  Check: 4679  from: 1  to: 1044



KIEE9421.orf

 With 182 enzymes: SgfI * 

                        December 27, 2011 16:39  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     246    1044 
   Size:        246     798
 
AflII C'TTAA_G

Cuts at:      0     990    1044 
   Size:        990      54
 
AflIII A'CryG_T

Cuts at:      0     325    1044 
   Size:        325     719
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0      30    1044 
   Size:         30    1014
 
AlwNI CAG_nnn'CTG

Cuts at:      0     311    1044 
   Size:        311     733
 
ApoI r'AATT_y

Cuts at:      0     316     852    1044 
   Size:        316     536     192

  Fragments arranged by size: 

                 536     316     192
 
BbvCI CC'TCA_GC

Cuts at:      0      23    1044 
   Size:         23    1021
 
BfrBI ATG'CAT

Cuts at:      0     455    1044 
   Size:        455     589
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     600    1044 
   Size:        600     444
 
Bpu10I CC'TnA_GC

Cuts at:      0      23    1044 
   Size:         23    1021
 
BsaBI GATnn'nnATC

Cuts at:      0     166    1044 
   Size:        166     878
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0      28     236     266    1044 
   Size:         28     208      30     778

  Fragments arranged by size: 

                 778     208      30      28
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     711     854    1044 
   Size:        711     143     190

  Fragments arranged by size: 

                 711     190     143
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     172    1044 
   Size:        172     872
 
BspHI T'CATG_A

Cuts at:      0     158    1044 
   Size:        158     886
 
BsrDI GCAATG_nn'

Cuts at:      0     392    1044 
   Size:        392     652
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     777     952    1044 
   Size:        777     175      92

  Fragments arranged by size: 

                 777     175      92
 
BtgI C'CryG_G

Cuts at:      0     635    1044 
   Size:        635     409
 
BtsI GCAGTG_nn'

Cuts at:      0     839    1044 
   Size:        839     205
 
DrdI GACnn_nn'nnGTC

Cuts at:      0    1002    1044 
   Size:       1002      42
 
EaeI y'GGCC_r

Cuts at:      0     668    1044 
   Size:        668     376
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     246    1044 
   Size:        246     798
 
EcoO109I rG'GnC_Cy

Cuts at:      0      88     594    1044 
   Size:         88     506     450

  Fragments arranged by size: 

                 506     450      88
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      86     118    1044 
   Size:         86      32     926

  Fragments arranged by size: 

                 926      86      32
 
HincII GTy'rAC

Cuts at:      0    1007    1044 
   Size:       1007      37
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     445    1044 
   Size:        445     599
 
MscI TGG'CCA

Cuts at:      0     670    1044 
   Size:        670     374
 
MslI CAynn'nnrTG

Cuts at:      0     253    1044 
   Size:        253     791
 
NcoI C'CATG_G

Cuts at:      0     635    1044 
   Size:        635     409
 
NsiI A_TGCA'T

Cuts at:      0     457    1044 
   Size:        457     587
 
NspI r_CATG'y

Cuts at:      0     329    1044 
   Size:        329     715
 
PciI A'CATG_T

Cuts at:      0     325    1044 
   Size:        325     719
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0      30    1044 
   Size:         30    1014
 
PpuMI rG'GwC_Cy

Cuts at:      0      88     594    1044 
   Size:         88     506     450

  Fragments arranged by size: 

                 506     450      88
 
PsiI TTA'TAA

Cuts at:      0     859     905    1044 
   Size:        859      46     139

  Fragments arranged by size: 

                 859     139      46
 
SmlI C'TyrA_G

Cuts at:      0     990    1044 
   Size:        990      54
 
StyI C'CwwG_G

Cuts at:      0     114     635     776    1044 
   Size:        114     521     141     268

  Fragments arranged by size: 

                 521     268     141     114
 
TatI w'GTAC_w

Cuts at:      0     697    1044 
   Size:        697     347
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     236     268    1044 
   Size:        236      32     776

  Fragments arranged by size: 

                 776     236      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     633    1044 
   Size:        633     411
 
XmnI GAAnn'nnTTC

Cuts at:      0      10    1044 
   Size:         10    1034
 

 Enzymes that do cut:

     AcuI    AflII   AflIII     AloI    AlwNI     ApoI    BbvCI    BfrBI
     BmrI   Bpu10I    BsaBI    BsaXI    BseRI     BsgI    BspHI    BsrDI
    BstXI     BtgI     BtsI     DrdI     EaeI  Eco57MI EcoO109I    Hin4I
   HincII     MmeI     MscI     MslI     NcoI     NsiI     NspI     PciI
     PpiI    PpuMI     PsiI     SmlI     StyI     TatI     TstI     XcmI
     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI     AgeI     AhdI
     AleI     AlfI     ApaI    ApaLI     AscI     AseI    AsiSI     AvaI
    AvrII     BaeI    BamHI     BanI    BanII     BbeI     BbsI     BcgI
    BciVI     BclI     BglI    BglII     BlpI Bme1580I    BmgBI     BmtI
     BplI     BpmI    BpuEI     BsaI    BsaAI    BsaHI    BsaWI    BseYI
    BsiEI  BsiHKAI    BsiWI     BsmI    BsmBI Bsp1286I    BspEI    BspMI
    BsrBI    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII
    BstYI  BstZ17I   Bsu36I    BtgZI     ClaI    CspCI     DraI   DraIII
     EagI     EarI     EciI  EcoICRI    EcoNI    EcoRI    EcoRV     FalI
     FseI     FspI    FspAI    HaeII  HindIII     HpaI     KasI     KpnI
     MfeI     MluI   MspA1I     NaeI     NarI     NdeI   NgoMIV     NheI
     NotI     NruI     PacI     PasI    PflMI     PfoI     PmeI     PmlI
    PshAI   PspOMI    PspXI     PsrI     PstI     PvuI    PvuII    RsrII
     SacI    SacII     SalI    SanDI     SapI     SbfI     ScaI    SexAI
     SfcI     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI
     SphI     SrfI     SspI     StuI     SwaI    TaqII  Tth111I     XbaI
     XhoI     XmaI     ZraI