(Linear) (Six-base) MAPSORT of: KIEE9417.seq  Check: 5263  from: 1  to: 1110



KIEE9417.orf

 With 182 enzymes: SgfI * 

                        December 27, 2011 16:39  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     568    1110 
   Size:        568     542
 
AccI GT'mk_AC

Cuts at:      0     134    1110 
   Size:        134     976
 
AflIII A'CryG_T

Cuts at:      0     582    1110 
   Size:        582     528
 
ApoI r'AATT_y

Cuts at:      0      42     459    1110 
   Size:         42     417     651

  Fragments arranged by size: 

                 651     417      42
 
AseI AT'TA_AT

Cuts at:      0    1090    1110 
   Size:       1090      20
 
BamHI G'GATC_C

Cuts at:      0     488    1110 
   Size:        488     622
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     180     598    1110 
   Size:        180     418     512

  Fragments arranged by size: 

                 512     418     180
 
BclI T'GATC_A

Cuts at:      0     749    1062    1110 
   Size:        749     313      48

  Fragments arranged by size: 

                 749     313      48
 
BglII A'GATC_T

Cuts at:      0     335    1110 
   Size:        335     775
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     982    1012    1110 
   Size:        982      30      98

  Fragments arranged by size: 

                 982      98      30
 
BsmI GAATG_Cn'

Cuts at:      0     349    1110 
   Size:        349     761
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     568    1110 
   Size:        568     542
 
BsrDI GCAATG_nn'

Cuts at:      0      75    1110 
   Size:         75    1035
 
BsrGI T'GTAC_A

Cuts at:      0     268    1110 
   Size:        268     842
 
BstYI r'GATC_y

Cuts at:      0     335     488    1110 
   Size:        335     153     622

  Fragments arranged by size: 

                 622     335     153
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     546     581    1110 
   Size:        546      35     529

  Fragments arranged by size: 

                 546     529      35
 
DraI TTT'AAA

Cuts at:      0     282    1110 
   Size:        282     828
 
DraIII CAC_nnn'GTG

Cuts at:      0     890    1110 
   Size:        890     220
 
EaeI y'GGCC_r

Cuts at:      0     413    1110 
   Size:        413     697
 
EarI CTCTTCn'nnn_

Cuts at:      0     675     695     811    1110 
   Size:        675      20     116     299

  Fragments arranged by size: 

                 675     299     116      20
 
EcoRI G'AATT_C

Cuts at:      0     459    1110 
   Size:        459     651
 
HincII GTy'rAC

Cuts at:      0     543    1110 
   Size:        543     567
 
MscI TGG'CCA

Cuts at:      0     415    1110 
   Size:        415     695
 
MslI CAynn'nnrTG

Cuts at:      0     205     985    1110 
   Size:        205     780     125

  Fragments arranged by size: 

                 780     205     125
 
NdeI CA'TA_TG

Cuts at:      0     761    1110 
   Size:        761     349
 
NspI r_CATG'y

Cuts at:      0     378    1110 
   Size:        378     732
 
SapI GCTCTTCn'nnn_

Cuts at:      0     675    1110 
   Size:        675     435
 
ScaI AGT'ACT

Cuts at:      0     740    1110 
   Size:        740     370
 
SfcI C'TryA_G

Cuts at:      0     135    1110 
   Size:        135     975
 
SphI G_CATG'C

Cuts at:      0     378    1110 
   Size:        378     732
 
SspI AAT'ATT

Cuts at:      0     287     617     653    1110 
   Size:        287     330      36     457

  Fragments arranged by size: 

                 457     330     287      36
 
TatI w'GTAC_w

Cuts at:      0     268     738     756    1110 
   Size:        268     470      18     354

  Fragments arranged by size: 

                 470     354     268      18
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     313    1110 
   Size:        313     797
 
XmnI GAAnn'nnTTC

Cuts at:      0     950     961    1110 
   Size:        950      11     149

  Fragments arranged by size: 

                 950     149      11
 

 Enzymes that do cut:

     AarI     AccI   AflIII     ApoI     AseI    BamHI     BbsI     BclI
    BglII    BsaXI     BsmI    BspMI    BsrDI    BsrGI    BstYI    CspCI
     DraI   DraIII     EaeI     EarI    EcoRI   HincII     MscI     MslI
     NdeI     NspI     SapI     ScaI     SfcI     SphI     SspI     TatI
     XcmI     XmnI

 Enzymes that do not cut: 

    AatII   Acc65I     AclI     AcuI     AfeI    AflII     AgeI     AhdI
     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI     AscI    AsiSI
     AvaI    AvrII     BaeI     BanI    BanII     BbeI    BbvCI     BcgI
    BciVI    BfrBI     BglI     BlpI Bme1580I    BmgBI     BmrI     BmtI
     BplI     BpmI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaHI
    BsaWI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI    BsiWI    BsmBI
 Bsp1286I    BspEI    BspHI    BsrBI    BsrFI   BssHII    BssSI   BstAPI
    BstBI   BstEII    BstXI  BstZ17I   Bsu36I     BtgI    BtgZI     BtsI
     ClaI     DrdI     EagI     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I
    EcoRV     FalI     FseI     FspI    FspAI    HaeII    Hin4I  HindIII
     HpaI     KasI     KpnI     MfeI     MluI     MmeI   MspA1I     NaeI
     NarI     NcoI   NgoMIV     NheI     NotI     NruI     NsiI     PacI
     PasI     PciI    PflMI     PfoI     PmeI     PmlI     PpiI    PpuMI
    PshAI     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI    PvuII
    RsrII     SacI    SacII     SalI    SanDI     SbfI    SexAI     SfiI
     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI     SrfI
     StuI     StyI     SwaI    TaqII     TstI  Tth111I     XbaI     XhoI
     XmaI     ZraI