Restriction map for
pFN21AE9416
(Linear) (Six-base) MAPSORT of: KIEE9416.seq Check: 8230 from: 1 to: 1620
KIEE9416.orf
With 182 enzymes: SgfI *
December 27, 2011 16:39 ..
AccI GT'mk_AC
Cuts at: 0 626 1620
Size: 626 994
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 4 82 198 303 418 1574 1620
Size: 4 78 116 105 115 1156 46
Fragments arranged by size:
1156 116 115 105 78 46 4
AflIII A'CryG_T
Cuts at: 0 1292 1620
Size: 1292 328
AleI CACnn'nnGTG
Cuts at: 0 124 307 967 1620
Size: 124 183 660 653
Fragments arranged by size:
660 653 183 124
AlwNI CAG_nnn'CTG
Cuts at: 0 684 1620
Size: 684 936
ApoI r'AATT_y
Cuts at: 0 220 1318 1620
Size: 220 1098 302
Fragments arranged by size:
1098 302 220
BanI G'GyrC_C
Cuts at: 0 1166 1620
Size: 1166 454
BbsI GAAGACnn'nnnn_
Cuts at: 0 88 180 406 559 994 1620
Size: 88 92 226 153 435 626
Fragments arranged by size:
626 435 226 153 92 88
BciVI GTATCCnnnnn_n'
Cuts at: 0 346 1620
Size: 346 1274
BclI T'GATC_A
Cuts at: 0 971 1620
Size: 971 649
BfrBI ATG'CAT
Cuts at: 0 1545 1620
Size: 1545 75
BglI GCCn_nnn'nGGC
Cuts at: 0 532 805 1620
Size: 532 273 815
Fragments arranged by size:
815 532 273
BglII A'GATC_T
Cuts at: 0 1376 1620
Size: 1376 244
BlpI GC'TnA_GC
Cuts at: 0 324 1620
Size: 324 1296
Bme1580I G_kGCm'C
Cuts at: 0 715 1620
Size: 715 905
BmrI ACTGGGnnnn_n'
Cuts at: 0 1018 1620
Size: 1018 602
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 70 892 1023 1620
Size: 70 822 131 597
Fragments arranged by size:
822 597 131 70
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 683 1620
Size: 683 937
BsaI GGTCTCn'nnnn_
Cuts at: 0 657 1620
Size: 657 963
BsaWI w'CCGG_w
Cuts at: 0 431 1620
Size: 431 1189
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1249 1279 1620
Size: 1249 30 341
Fragments arranged by size:
1249 341 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 478 1492 1620
Size: 478 1014 128
Fragments arranged by size:
1014 478 128
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 406 462 790 1160 1591 1620
Size: 406 56 328 370 431 29
Fragments arranged by size:
431 406 370 328 56 29
BsiHKAI G_wGCw'C
Cuts at: 0 1163 1620
Size: 1163 457
Bsp1286I G_dGCh'C
Cuts at: 0 715 1163 1620
Size: 715 448 457
Fragments arranged by size:
715 457 448
BspEI T'CCGG_A
Cuts at: 0 431 1620
Size: 431 1189
BspHI T'CATG_A
Cuts at: 0 242 668 1272 1620
Size: 242 426 604 348
Fragments arranged by size:
604 426 348 242
BsrDI GCAATG_nn'
Cuts at: 0 1050 1620
Size: 1050 570
BsrFI r'CCGG_y
Cuts at: 0 425 804 1620
Size: 425 379 816
Fragments arranged by size:
816 425 379
BstEII G'GTnAC_C
Cuts at: 0 126 885 960 1620
Size: 126 759 75 660
Fragments arranged by size:
759 660 126 75
BstXI CCAn_nnnn'nTGG
Cuts at: 0 882 957 1336 1620
Size: 882 75 379 284
Fragments arranged by size:
882 379 284 75
BstYI r'GATC_y
Cuts at: 0 102 783 1376 1620
Size: 102 681 593 244
Fragments arranged by size:
681 593 244 102
BtgI C'CryG_G
Cuts at: 0 855 956 1226 1620
Size: 855 101 270 394
Fragments arranged by size:
855 394 270 101
BtsI GCAGTG_nn'
Cuts at: 0 416 935 1620
Size: 416 519 685
Fragments arranged by size:
685 519 416
EarI CTCTTCn'nnn_
Cuts at: 0 277 1620
Size: 277 1343
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 4 70 82 198 303 418 892 1023
Size: 4 66 12 116 105 115 474 131
Cuts at: 1023 1574 1620
Size: 551 46
Fragments arranged by size:
551 474 131 116 115 105 66 46
12 4
EcoO109I rG'GnC_Cy
Cuts at: 0 762 1620
Size: 762 858
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 773 805 896 928 1620
Size: 773 32 91 32 692
Fragments arranged by size:
773 692 91 32 32
HincII GTy'rAC
Cuts at: 0 627 1251 1620
Size: 627 624 369
Fragments arranged by size:
627 624 369
MslI CAynn'nnrTG
Cuts at: 0 124 307 388 967 1620
Size: 124 183 81 579 653
Fragments arranged by size:
653 579 183 124 81
NaeI GCC'GGC
Cuts at: 0 806 1620
Size: 806 814
NcoI C'CATG_G
Cuts at: 0 1226 1620
Size: 1226 394
NgoMIV G'CCGG_C
Cuts at: 0 804 1620
Size: 804 816
NsiI A_TGCA'T
Cuts at: 0 1547 1620
Size: 1547 73
NspI r_CATG'y
Cuts at: 0 1296 1620
Size: 1296 324
PciI A'CATG_T
Cuts at: 0 1292 1620
Size: 1292 328
PfoI T'CCnGG_A
Cuts at: 0 86 338 1620
Size: 86 252 1282
Fragments arranged by size:
1282 252 86
PpuMI rG'GwC_Cy
Cuts at: 0 762 1620
Size: 762 858
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 811 843 1016 1048 1620
Size: 811 32 173 32 572
Fragments arranged by size:
811 572 173 32 32
PstI C_TGCA'G
Cuts at: 0 842 897 1620
Size: 842 55 723
Fragments arranged by size:
842 723 55
SalI G'TCGA_C
Cuts at: 0 625 1620
Size: 625 995
SanDI GG'GwC_CC
Cuts at: 0 762 1620
Size: 762 858
SfcI C'TryA_G
Cuts at: 0 838 893 1446 1620
Size: 838 55 553 174
Fragments arranged by size:
838 553 174 55
SmlI C'TyrA_G
Cuts at: 0 698 1620
Size: 698 922
StuI AGG'CCT
Cuts at: 0 1184 1620
Size: 1184 436
StyI C'CwwG_G
Cuts at: 0 122 530 941 1019 1226 1620
Size: 122 408 411 78 207 394
Fragments arranged by size:
411 408 394 207 122 78
TatI w'GTAC_w
Cuts at: 0 133 1620
Size: 133 1487
Enzymes that do cut:
AccI AcuI AflIII AleI AlwNI ApoI BanI BbsI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmrI
BpmI BpuEI BsaI BsaWI BsaXI BseRI BsgI BsiHKAI
Bsp1286I BspEI BspHI BsrDI BsrFI BstEII BstXI BstYI
BtgI BtsI EarI Eco57MI EcoO109I Hin4I HincII MslI
NaeI NcoI NgoMIV NsiI NspI PciI PfoI PpuMI
PsrI PstI SalI SanDI SfcI SmlI StuI StyI
TatI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AgeI AhdI
AlfI AloI ApaI ApaLI AscI AseI AsiSI AvaI
AvrII BaeI BamHI BanII BbeI BbvCI BcgI BmgBI
BmtI BplI Bpu10I BsaAI BsaBI BsaHI BseYI BsiEI
BsiWI BsmI BsmBI BspMI BsrBI BsrGI BssHII BssSI
BstAPI BstBI BstZ17I Bsu36I BtgZI ClaI CspCI DraI
DraIII DrdI EaeI EagI EciI EcoICRI EcoNI EcoRI
EcoRV FalI FseI FspI FspAI HaeII HindIII HpaI
KasI KpnI MfeI MluI MmeI MscI MspA1I NarI
NdeI NheI NotI NruI PacI PasI PflMI PmeI
PmlI PpiI PshAI PsiI PspOMI PspXI PvuI PvuII
RsrII SacI SacII SapI SbfI ScaI SexAI SfiI
SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI
SspI SwaI TaqII TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI