(Linear) (Six-base) MAPSORT of: KIEE9414.seq  Check: 203  from: 1  to: 1083



KIEE9414.orf

 With 182 enzymes: SgfI * 

                        December 27, 2011 16:39  ..

AleI CACnn'nnGTG

Cuts at:      0     101    1083 
   Size:        101     982
 
ApaLI G'TGCA_C

Cuts at:      0    1005    1083 
   Size:       1005      78
 
ApoI r'AATT_y

Cuts at:      0     476     683    1083 
   Size:        476     207     400

  Fragments arranged by size: 

                 476     400     207
 
AvaI C'yCGr_G

Cuts at:      0     869    1083 
   Size:        869     214
 
AvrII C'CTAG_G

Cuts at:      0    1054    1083 
   Size:       1054      29
 
BamHI G'GATC_C

Cuts at:      0      29    1083 
   Size:         29    1054
 
BanII G_rGCy'C

Cuts at:      0     726    1083 
   Size:        726     357
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     993    1083 
   Size:        993      90
 
BclI T'GATC_A

Cuts at:      0     158    1083 
   Size:        158     925
 
Bme1580I G_kGCm'C

Cuts at:      0    1009    1083 
   Size:       1009      74
 
BmgBI CAC'GTC

Cuts at:      0     584    1083 
   Size:        584     499
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0      90    1083 
   Size:         90     993
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     492    1083 
   Size:        492     591
 
BsaBI GATnn'nnATC

Cuts at:      0     416     496    1083 
   Size:        416      80     587

  Fragments arranged by size: 

                 587     416      80
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     940     970    1083 
   Size:        940      30     113

  Fragments arranged by size: 

                 940     113      30
 
BseYI C'CCAG_C

Cuts at:      0     166     536    1083 
   Size:        166     370     547

  Fragments arranged by size: 

                 547     370     166
 
BsiHKAI G_wGCw'C

Cuts at:      0     204    1009    1083 
   Size:        204     805      74

  Fragments arranged by size: 

                 805     204      74
 
Bsp1286I G_dGCh'C

Cuts at:      0     204     726    1009    1083 
   Size:        204     522     283      74

  Fragments arranged by size: 

                 522     283     204      74
 
BspHI T'CATG_A

Cuts at:      0     738    1083 
   Size:        738     345
 
BsrDI GCAATG_nn'

Cuts at:      0     161    1083 
   Size:        161     922
 
BssSI C'ACGA_G

Cuts at:      0     325    1083 
   Size:        325     758
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     394    1083 
   Size:        394     689
 
BstEII G'GTnAC_C

Cuts at:      0     456     550    1083 
   Size:        456      94     533

  Fragments arranged by size: 

                 533     456      94
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     349    1083 
   Size:        349     734
 
BstYI r'GATC_y

Cuts at:      0      29     497    1083 
   Size:         29     468     586

  Fragments arranged by size: 

                 586     468      29
 
BtgI C'CryG_G

Cuts at:      0     555     791    1083 
   Size:        555     236     292

  Fragments arranged by size: 

                 555     292     236
 
BtsI GCAGTG_nn'

Cuts at:      0      51    1083 
   Size:         51    1032
 
EaeI y'GGCC_r

Cuts at:      0      93    1083 
   Size:         93     990
 
EarI CTCTTCn'nnn_

Cuts at:      0     714    1083 
   Size:        714     369
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     344     376    1083 
   Size:        344      32     707

  Fragments arranged by size: 

                 707     344      32
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     249     910     983    1083 
   Size:        249     661      73     100

  Fragments arranged by size: 

                 661     249     100      73
 
MscI TGG'CCA

Cuts at:      0      95    1083 
   Size:         95     988
 
MslI CAynn'nnrTG

Cuts at:      0     101     406    1083 
   Size:        101     305     677

  Fragments arranged by size: 

                 677     305     101
 
NcoI C'CATG_G

Cuts at:      0     555     791    1083 
   Size:        555     236     292

  Fragments arranged by size: 

                 555     292     236
 
NdeI CA'TA_TG

Cuts at:      0     252    1083 
   Size:        252     831
 
NspI r_CATG'y

Cuts at:      0     392    1083 
   Size:        392     691
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     587    1083 
   Size:        587     496
 
SapI GCTCTTCn'nnn_

Cuts at:      0     714    1083 
   Size:        714     369
 
SexAI A'CCwGG_T

Cuts at:      0     546    1083 
   Size:        546     537
 
SfcI C'TryA_G

Cuts at:      0      99    1083 
   Size:         99     984
 
SmaI CCC'GGG

Cuts at:      0     871    1083 
   Size:        871     212
 
SmlI C'TyrA_G

Cuts at:      0     471    1083 
   Size:        471     612
 
StuI AGG'CCT

Cuts at:      0     980    1083 
   Size:        980     103
 
StyI C'CwwG_G

Cuts at:      0     555     791    1054    1083 
   Size:        555     236     263      29

  Fragments arranged by size: 

                 555     263     236      29
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     731    1083 
   Size:        731     352
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     798     830    1083 
   Size:        798      32     253

  Fragments arranged by size: 

                 798     253      32
 
XbaI T'CTAG_A

Cuts at:      0     858    1083 
   Size:        858     225
 
XmaI C'CCGG_G

Cuts at:      0     869    1083 
   Size:        869     214
 

 Enzymes that do cut:

     AleI    ApaLI     ApoI     AvaI    AvrII    BamHI    BanII     BbsI
     BclI Bme1580I    BmgBI     BplI    BpuEI    BsaBI    BsaXI    BseYI
  BsiHKAI Bsp1286I    BspHI    BsrDI    BssSI   BstAPI   BstEII    BstXI
    BstYI     BtgI     BtsI     EaeI     EarI    Hin4I     MmeI     MscI
     MslI     NcoI     NdeI     NspI    PflMI     SapI    SexAI     SfcI
     SmaI     SmlI     StuI     StyI    TaqII     TstI     XbaI     XmaI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AlfI     AloI    AlwNI     ApaI     AscI
     AseI    AsiSI     BaeI     BanI     BbeI    BbvCI     BcgI    BciVI
    BfrBI     BglI    BglII     BlpI     BmrI     BmtI     BpmI   Bpu10I
     BsaI    BsaAI    BsaHI    BsaWI    BseRI     BsgI    BsiEI    BsiWI
     BsmI    BsmBI    BspEI    BspMI    BsrBI    BsrFI    BsrGI   BssHII
    BstBI  BstZ17I   Bsu36I    BtgZI     ClaI    CspCI     DraI   DraIII
     DrdI     EagI     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I    EcoRI
    EcoRV     FalI     FseI     FspI    FspAI    HaeII   HincII  HindIII
     HpaI     KasI     KpnI     MfeI     MluI   MspA1I     NaeI     NarI
   NgoMIV     NheI     NotI     NruI     NsiI     PacI     PasI     PciI
     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI
    PspXI     PsrI     PstI     PvuI    PvuII    RsrII     SacI    SacII
     SalI    SanDI     SbfI     ScaI     SfiI     SfoI     SgfI    SgrAI
    SnaBI     SpeI     SphI     SrfI     SspI     SwaI     TatI  Tth111I
     XcmI     XhoI     XmnI     ZraI