Restriction map for
pFN21AE9412
(Linear) (Six-base) MAPSORT of: KIEE9412.seq Check: 718 from: 1 to: 927
KIEE9412.orf
With 182 enzymes: SgfI *
December 27, 2011 16:39 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 471 595 927
Size: 471 124 332
Fragments arranged by size:
471 332 124
AhdI GACnn_n'nnGTC
Cuts at: 0 761 927
Size: 761 166
AleI CACnn'nnGTG
Cuts at: 0 613 927
Size: 613 314
ApaLI G'TGCA_C
Cuts at: 0 210 927
Size: 210 717
BanI G'GyrC_C
Cuts at: 0 7 356 927
Size: 7 349 571
Fragments arranged by size:
571 349 7
BbsI GAAGACnn'nnnn_
Cuts at: 0 555 927
Size: 555 372
Bme1580I G_kGCm'C
Cuts at: 0 214 372 747 927
Size: 214 158 375 180
Fragments arranged by size:
375 214 180 158
BmgBI CAC'GTC
Cuts at: 0 615 927
Size: 615 312
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 12 772 927
Size: 12 760 155
Fragments arranged by size:
760 155 12
Bpu10I CC'TnA_GC
Cuts at: 0 884 927
Size: 884 43
BsaI GGTCTCn'nnnn_
Cuts at: 0 154 927
Size: 154 773
BsaAI yAC'GTr
Cuts at: 0 702 927
Size: 702 225
BsaHI Gr'CG_yC
Cuts at: 0 83 815 856 927
Size: 83 732 41 71
Fragments arranged by size:
732 83 71 41
BseYI C'CCAG_C
Cuts at: 0 48 60 531 927
Size: 48 12 471 396
Fragments arranged by size:
471 396 48 12
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 214 682 845 927
Size: 214 468 163 82
Fragments arranged by size:
468 214 163 82
BsiEI CG_ry'CG
Cuts at: 0 404 927
Size: 404 523
BsiHKAI G_wGCw'C
Cuts at: 0 214 496 927
Size: 214 282 431
Fragments arranged by size:
431 282 214
BsmBI CGTCTCn'nnnn_
Cuts at: 0 748 927
Size: 748 179
Bsp1286I G_dGCh'C
Cuts at: 0 214 372 496 747 927
Size: 214 158 124 251 180
Fragments arranged by size:
251 214 180 158 124
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 551 927
Size: 551 376
BsrGI T'GTAC_A
Cuts at: 0 773 927
Size: 773 154
BstAPI GCAn_nnn'nTGC
Cuts at: 0 237 363 927
Size: 237 126 564
Fragments arranged by size:
564 237 126
BstXI CCAn_nnnn'nTGG
Cuts at: 0 333 615 669 927
Size: 333 282 54 258
Fragments arranged by size:
333 282 258 54
BtgI C'CryG_G
Cuts at: 0 332 372 515 554 869 927
Size: 332 40 143 39 315 58
Fragments arranged by size:
332 315 143 58 40 39
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 312 927
Size: 312 615
BtsI GCAGTG_nn'
Cuts at: 0 157 731 927
Size: 157 574 196
Fragments arranged by size:
574 196 157
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 3 38 927
Size: 3 35 889
Fragments arranged by size:
889 35 3
EaeI y'GGCC_r
Cuts at: 0 191 323 347 401 551 836 866 927
Size: 191 132 24 54 150 285 30 61
Fragments arranged by size:
285 191 150 132 61 54 30 24
EagI C'GGCC_G
Cuts at: 0 401 927
Size: 401 526
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 12 471 595 772 927
Size: 12 459 124 177 155
Fragments arranged by size:
459 177 155 124 12
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 81 927
Size: 81 846
HaeII r_GCGC'y
Cuts at: 0 124 927
Size: 124 803
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 747 779 927
Size: 747 32 148
Fragments arranged by size:
747 148 32
MscI TGG'CCA
Cuts at: 0 838 927
Size: 838 89
MslI CAynn'nnrTG
Cuts at: 0 172 331 613 927
Size: 172 159 282 314
Fragments arranged by size:
314 282 172 159
MspA1I CmG'CkG
Cuts at: 0 261 363 374 528 927
Size: 261 102 11 154 399
Fragments arranged by size:
399 261 154 102 11
NcoI C'CATG_G
Cuts at: 0 515 927
Size: 515 412
NspI r_CATG'y
Cuts at: 0 147 927
Size: 147 780
PasI CC'CwG_GG
Cuts at: 0 276 927
Size: 276 651
PfoI T'CCnGG_A
Cuts at: 0 749 848 927
Size: 749 99 79
Fragments arranged by size:
749 99 79
PmlI CAC'GTG
Cuts at: 0 702 927
Size: 702 225
SacII CC_GC'GG
Cuts at: 0 375 927
Size: 375 552
SphI G_CATG'C
Cuts at: 0 147 927
Size: 147 780
StuI AGG'CCT
Cuts at: 0 101 927
Size: 101 826
StyI C'CwwG_G
Cuts at: 0 515 839 927
Size: 515 324 88
Fragments arranged by size:
515 324 88
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 117 411 621 927
Size: 117 294 210 306
Fragments arranged by size:
306 294 210 117
TatI w'GTAC_w
Cuts at: 0 773 927
Size: 773 154
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 726 758 895 927
Size: 726 32 137 32
Fragments arranged by size:
726 137 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 186 807 927
Size: 186 621 120
Fragments arranged by size:
621 186 120
Enzymes that do cut:
AcuI AhdI AleI ApaLI BanI BbsI Bme1580I BmgBI
BpmI Bpu10I BsaI BsaAI BsaHI BseYI BsgI BsiEI
BsiHKAI BsmBI Bsp1286I BspMI BsrGI BstAPI BstXI BtgI
BtgZI BtsI CspCI EaeI EagI Eco57MI FalI HaeII
Hin4I MscI MslI MspA1I NcoI NspI PasI PfoI
PmlI SacII SphI StuI StyI TaqII TatI TstI
XcmI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AlfI AloI AlwNI ApaI ApoI AscI AseI
AsiSI AvaI AvrII BaeI BamHI BanII BbeI BbvCI
BcgI BciVI BclI BfrBI BglI BglII BlpI BmrI
BmtI BplI BpuEI BsaBI BsaWI BsaXI BseRI BsiWI
BsmI BspEI BspHI BsrBI BsrDI BsrFI BssHII BssSI
BstBI BstEII BstYI BstZ17I Bsu36I ClaI DraI DraIII
DrdI EarI EciI EcoICRI EcoNI EcoO109I EcoRI EcoRV
FseI FspI FspAI HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI NaeI NarI NdeI NgoMIV NheI
NotI NruI NsiI PacI PciI PflMI PmeI PpiI
PpuMI PshAI PsiI PspOMI PspXI PsrI PstI PvuI
PvuII RsrII SacI SalI SanDI SapI SbfI ScaI
SexAI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SrfI SspI SwaI Tth111I XbaI XhoI
XmaI XmnI ZraI