Restriction map for
pFN21AE9411
(Linear) (Six-base) MAPSORT of: KIEE9411.seq Check: 5183 from: 1 to: 1068
KIEE9411.orf
With 182 enzymes: SgfI *
December 27, 2011 16:39 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 709 1068
Size: 709 359
AfeI AGC'GCT
Cuts at: 0 789 1068
Size: 789 279
AflIII A'CryG_T
Cuts at: 0 58 1068
Size: 58 1010
AleI CACnn'nnGTG
Cuts at: 0 358 1068
Size: 358 710
AlwNI CAG_nnn'CTG
Cuts at: 0 29 386 1068
Size: 29 357 682
Fragments arranged by size:
682 357 29
ApaI G_GGCC'C
Cuts at: 0 447 683 1068
Size: 447 236 385
Fragments arranged by size:
447 385 236
ApoI r'AATT_y
Cuts at: 0 532 1068
Size: 532 536
AvaI C'yCGr_G
Cuts at: 0 1061 1068
Size: 1061 7
BanI G'GyrC_C
Cuts at: 0 697 1068
Size: 697 371
BanII G_rGCy'C
Cuts at: 0 447 683 1068
Size: 447 236 385
Fragments arranged by size:
447 385 236
BbsI GAAGACnn'nnnn_
Cuts at: 0 198 390 942 1068
Size: 198 192 552 126
Fragments arranged by size:
552 198 192 126
BbvCI CC'TCA_GC
Cuts at: 0 367 1068
Size: 367 701
BglI GCCn_nnn'nGGC
Cuts at: 0 765 1068
Size: 765 303
Bme1580I G_kGCm'C
Cuts at: 0 447 683 1068
Size: 447 236 385
Fragments arranged by size:
447 385 236
BmtI G_CTAG'C
Cuts at: 0 902 1068
Size: 902 166
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 294 877 1068
Size: 294 583 191
Fragments arranged by size:
583 294 191
Bpu10I CC'TnA_GC
Cuts at: 0 367 684 1068
Size: 367 317 384
Fragments arranged by size:
384 367 317
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 841 1068
Size: 841 227
BsaI GGTCTCn'nnnn_
Cuts at: 0 15 231 1068
Size: 15 216 837
Fragments arranged by size:
837 216 15
BsaAI yAC'GTr
Cuts at: 0 61 1068
Size: 61 1007
BsaWI w'CCGG_w
Cuts at: 0 94 536 1068
Size: 94 442 532
Fragments arranged by size:
532 442 94
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 103 133 1018 1048 1068
Size: 103 30 885 30 20
Fragments arranged by size:
885 103 30 30 20
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 124 313 1036 1039 1068
Size: 124 189 723 3 29
Fragments arranged by size:
723 189 124 29 3
BseYI C'CCAG_C
Cuts at: 0 174 666 740 1068
Size: 174 492 74 328
Fragments arranged by size:
492 328 174 74
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 484 1068
Size: 484 584
BsiHKAI G_wGCw'C
Cuts at: 0 520 1068
Size: 520 548
Bsp1286I G_dGCh'C
Cuts at: 0 447 520 683 1068
Size: 447 73 163 385
Fragments arranged by size:
447 385 163 73
BspEI T'CCGG_A
Cuts at: 0 94 536 1068
Size: 94 442 532
Fragments arranged by size:
532 442 94
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 709 1068
Size: 709 359
BsrDI GCAATG_nn'
Cuts at: 0 186 287 1068
Size: 186 101 781
Fragments arranged by size:
781 186 101
BsrFI r'CCGG_y
Cuts at: 0 755 1068
Size: 755 313
BtgI C'CryG_G
Cuts at: 0 1047 1068
Size: 1047 21
BtsI GCAGTG_nn'
Cuts at: 0 39 322 567 1068
Size: 39 283 245 501
Fragments arranged by size:
501 283 245 39
EaeI y'GGCC_r
Cuts at: 0 503 654 1068
Size: 503 151 414
Fragments arranged by size:
503 414 151
EarI CTCTTCn'nnn_
Cuts at: 0 96 1068
Size: 96 972
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 115 1068
Size: 115 953
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 294 877 1068
Size: 294 583 191
Fragments arranged by size:
583 294 191
EcoNI CCTnn'n_nnAGG
Cuts at: 0 106 1068
Size: 106 962
EcoO109I rG'GnC_Cy
Cuts at: 0 51 119 444 679 680 1068
Size: 51 68 325 235 1 388
Fragments arranged by size:
388 325 235 68 51 1
FspI TGC'GCA
Cuts at: 0 524 1068
Size: 524 544
HaeII r_GCGC'y
Cuts at: 0 791 1068
Size: 791 277
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 48 80 103 135 1018 1050 1068
Size: 48 32 23 32 883 32 18
Fragments arranged by size:
883 48 32 32 32 23 18
HincII GTy'rAC
Cuts at: 0 921 1068
Size: 921 147
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 147 1068
Size: 147 921
MscI TGG'CCA
Cuts at: 0 656 1068
Size: 656 412
MslI CAynn'nnrTG
Cuts at: 0 292 358 406 1068
Size: 292 66 48 662
Fragments arranged by size:
662 292 66 48
MspA1I CmG'CkG
Cuts at: 0 468 1068
Size: 468 600
NheI G'CTAG_C
Cuts at: 0 898 1068
Size: 898 170
PflMI CCAn_nnn'nTGG
Cuts at: 0 317 551 1068
Size: 317 234 517
Fragments arranged by size:
517 317 234
PfoI T'CCnGG_A
Cuts at: 0 76 1068
Size: 76 992
PmlI CAC'GTG
Cuts at: 0 61 1068
Size: 61 1007
PpuMI rG'GwC_Cy
Cuts at: 0 51 119 1068
Size: 51 68 949
Fragments arranged by size:
949 68 51
PspOMI G'GGCC_C
Cuts at: 0 443 679 1068
Size: 443 236 389
Fragments arranged by size:
443 389 236
PstI C_TGCA'G
Cuts at: 0 34 1068
Size: 34 1034
SanDI GG'GwC_CC
Cuts at: 0 119 1068
Size: 119 949
SfcI C'TryA_G
Cuts at: 0 30 1068
Size: 30 1038
SmaI CCC'GGG
Cuts at: 0 1063 1068
Size: 1063 5
SmlI C'TyrA_G
Cuts at: 0 856 1068
Size: 856 212
StyI C'CwwG_G
Cuts at: 0 908 1068
Size: 908 160
TatI w'GTAC_w
Cuts at: 0 296 1068
Size: 296 772
XmaI C'CCGG_G
Cuts at: 0 1061 1068
Size: 1061 7
Enzymes that do cut:
AarI AfeI AflIII AleI AlwNI ApaI ApoI AvaI
BanI BanII BbsI BbvCI BglI Bme1580I BmtI BpmI
Bpu10I BpuEI BsaI BsaAI BsaWI BsaXI BseRI BseYI
BsgI BsiHKAI Bsp1286I BspEI BspMI BsrDI BsrFI BtgI
BtsI EaeI EarI EciI Eco57MI EcoNI EcoO109I FspI
HaeII Hin4I HincII MmeI MscI MslI MspA1I NheI
PflMI PfoI PmlI PpuMI PspOMI PstI SanDI SfcI
SmaI SmlI StyI TatI XmaI
Enzymes that do not cut:
AatII AccI Acc65I AclI AcuI AflII AgeI AhdI
AlfI AloI ApaLI AscI AseI AsiSI AvrII BaeI
BamHI BbeI BcgI BciVI BclI BfrBI BglII BlpI
BmgBI BmrI BplI BsaBI BsaHI BsiEI BsiWI BsmI
BsmBI BspHI BsrBI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI BstZ17I Bsu36I BtgZI ClaI CspCI
DraI DraIII DrdI EagI EcoICRI EcoRI EcoRV FalI
FseI FspAI HindIII HpaI KasI KpnI MfeI MluI
NaeI NarI NcoI NdeI NgoMIV NotI NruI NsiI
NspI PacI PasI PciI PmeI PpiI PshAI PsiI
PspXI PsrI PvuI PvuII RsrII SacI SacII SalI
SapI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI
SnaBI SpeI SphI SrfI SspI StuI SwaI TaqII
TstI Tth111I XbaI XcmI XhoI XmnI ZraI