Restriction map for
pFN21AE9410
(Linear) (Six-base) MAPSORT of: KIEE9410.seq Check: 4743 from: 1 to: 957
KIEE9410.orf
With 182 enzymes: SgfI *
July 26, 2011 17:41 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 445 957
Size: 445 512
AccI GT'mk_AC
Cuts at: 0 152 842 957
Size: 152 690 115
Fragments arranged by size:
690 152 115
Acc65I G'GTAC_C
Cuts at: 0 335 957
Size: 335 622
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 501 535 634 849 957
Size: 501 34 99 215 108
Fragments arranged by size:
501 215 108 99 34
AflII C'TTAA_G
Cuts at: 0 361 957
Size: 361 596
AgeI A'CCGG_T
Cuts at: 0 332 957
Size: 332 625
AleI CACnn'nnGTG
Cuts at: 0 34 46 676 957
Size: 34 12 630 281
Fragments arranged by size:
630 281 34 12
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 561 957
Size: 561 396
AlwNI CAG_nnn'CTG
Cuts at: 0 666 750 957
Size: 666 84 207
Fragments arranged by size:
666 207 84
ApaI G_GGCC'C
Cuts at: 0 267 660 768 957
Size: 267 393 108 189
Fragments arranged by size:
393 267 189 108
AvaI C'yCGr_G
Cuts at: 0 402 441 957
Size: 402 39 516
Fragments arranged by size:
516 402 39
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 327 360 957
Size: 327 33 597
Fragments arranged by size:
597 327 33
BanI G'GyrC_C
Cuts at: 0 335 482 957
Size: 335 147 475
Fragments arranged by size:
475 335 147
BanII G_rGCy'C
Cuts at: 0 267 609 660 768 874 926 957
Size: 267 342 51 108 106 52 31
Fragments arranged by size:
342 267 108 106 52 51 31
BbsI GAAGACnn'nnnn_
Cuts at: 0 879 957
Size: 879 78
BbvCI CC'TCA_GC
Cuts at: 0 286 957
Size: 286 671
BglI GCCn_nnn'nGGC
Cuts at: 0 254 313 322 957
Size: 254 59 9 635
Fragments arranged by size:
635 254 59 9
BlpI GC'TnA_GC
Cuts at: 0 18 957
Size: 18 939
Bme1580I G_kGCm'C
Cuts at: 0 267 660 768 957
Size: 267 393 108 189
Fragments arranged by size:
393 267 189 108
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 88 214 483 595 837 957
Size: 88 126 269 112 242 120
Fragments arranged by size:
269 242 126 120 112 88
Bpu10I CC'TnA_GC
Cuts at: 0 223 286 957
Size: 223 63 671
Fragments arranged by size:
671 223 63
BsaI GGTCTCn'nnnn_
Cuts at: 0 405 957
Size: 405 552
BsaBI GATnn'nnATC
Cuts at: 0 736 957
Size: 736 221
BsaWI w'CCGG_w
Cuts at: 0 332 957
Size: 332 625
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 835 865 957
Size: 835 30 92
Fragments arranged by size:
835 92 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 371 957
Size: 371 586
BseYI C'CCAG_C
Cuts at: 0 25 60 78 87 260 957
Size: 25 35 18 9 173 697
Fragments arranged by size:
697 173 35 25 18 9
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 794 802 957
Size: 794 8 155
Fragments arranged by size:
794 155 8
BsiHKAI G_wGCw'C
Cuts at: 0 830 874 957
Size: 830 44 83
Fragments arranged by size:
830 83 44
BsiWI C'GTAC_G
Cuts at: 0 143 957
Size: 143 814
Bsp1286I G_dGCh'C
Cuts at: 0 267 609 660 768 830 874 926 957
Size: 267 342 51 108 62 44 52 31
Fragments arranged by size:
342 267 108 62 52 51 44 31
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 164 188 380 445 824 957
Size: 164 24 192 65 379 133
Fragments arranged by size:
379 192 164 133 65 24
BsrDI GCAATG_nn'
Cuts at: 0 594 957
Size: 594 363
BsrFI r'CCGG_y
Cuts at: 0 332 957
Size: 332 625
BstAPI GCAn_nnn'nTGC
Cuts at: 0 825 957
Size: 825 132
BstEII G'GTnAC_C
Cuts at: 0 669 957
Size: 669 288
BstXI CCAn_nnnn'nTGG
Cuts at: 0 33 48 957
Size: 33 15 909
Fragments arranged by size:
909 33 15
BstZ17I GTA'TAC
Cuts at: 0 843 957
Size: 843 114
Bsu36I CC'TnA_GG
Cuts at: 0 383 957
Size: 383 574
BtgI C'CryG_G
Cuts at: 0 320 836 957
Size: 320 516 121
Fragments arranged by size:
516 320 121
BtsI GCAGTG_nn'
Cuts at: 0 782 957
Size: 782 175
DraIII CAC_nnn'GTG
Cuts at: 0 482 825 957
Size: 482 343 132
Fragments arranged by size:
482 343 132
EaeI y'GGCC_r
Cuts at: 0 38 209 314 719 957
Size: 38 171 105 405 238
Fragments arranged by size:
405 238 171 105 38
EcoICRI GAG'CTC
Cuts at: 0 872 957
Size: 872 85
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 88 214 483 501 535 595 634 837
Size: 88 126 269 18 34 60 39 203
Cuts at: 837 849 957
Size: 12 108
Fragments arranged by size:
269 203 126 108 88 60 39 34
18 12
EcoNI CCTnn'n_nnAGG
Cuts at: 0 879 957
Size: 879 78
EcoO109I rG'GnC_Cy
Cuts at: 0 264 764 765 957
Size: 264 500 1 192
Fragments arranged by size:
500 264 192 1
HaeII r_GCGC'y
Cuts at: 0 106 957
Size: 106 851
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 861 893 957
Size: 861 32 64
Fragments arranged by size:
861 64 32
HincII GTy'rAC
Cuts at: 0 369 957
Size: 369 588
HindIII A'AGCT_T
Cuts at: 0 646 957
Size: 646 311
KpnI G_GTAC'C
Cuts at: 0 339 957
Size: 339 618
MscI TGG'CCA
Cuts at: 0 40 721 957
Size: 40 681 236
Fragments arranged by size:
681 236 40
MslI CAynn'nnrTG
Cuts at: 0 34 46 676 957
Size: 34 12 630 281
Fragments arranged by size:
630 281 34 12
MspA1I CmG'CkG
Cuts at: 0 46 654 957
Size: 46 608 303
Fragments arranged by size:
608 303 46
PasI CC'CwG_GG
Cuts at: 0 393 957
Size: 393 564
PflMI CCAn_nnn'nTGG
Cuts at: 0 666 750 957
Size: 666 84 207
Fragments arranged by size:
666 207 84
PfoI T'CCnGG_A
Cuts at: 0 623 957
Size: 623 334
PspOMI G'GGCC_C
Cuts at: 0 263 656 764 957
Size: 263 393 108 193
Fragments arranged by size:
393 263 193 108
PstI C_TGCA'G
Cuts at: 0 763 957
Size: 763 194
PvuII CAG'CTG
Cuts at: 0 46 957
Size: 46 911
SacI G_AGCT'C
Cuts at: 0 874 957
Size: 874 83
SexAI A'CCwGG_T
Cuts at: 0 478 957
Size: 478 479
SfcI C'TryA_G
Cuts at: 0 759 957
Size: 759 198
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 313 957
Size: 313 644
SmaI CCC'GGG
Cuts at: 0 443 957
Size: 443 514
SmlI C'TyrA_G
Cuts at: 0 361 957
Size: 361 596
SrfI GCCC'GGGC
Cuts at: 0 443 957
Size: 443 514
StyI C'CwwG_G
Cuts at: 0 674 957
Size: 674 283
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 40 957
Size: 40 917
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 33 957
Size: 33 924
XmaI C'CCGG_G
Cuts at: 0 441 957
Size: 441 516
Enzymes that do cut:
AarI AccI Acc65I AcuI AflII AgeI AleI AlfI
AlwNI ApaI AvaI BaeI BanI BanII BbsI BbvCI
BglI BlpI Bme1580I BpmI Bpu10I BsaI BsaBI BsaWI
BsaXI BseRI BseYI BsgI BsiHKAI BsiWI Bsp1286I BspMI
BsrDI BsrFI BstAPI BstEII BstXI BstZ17I Bsu36I BtgI
BtsI DraIII EaeI EcoICRI Eco57MI EcoNI EcoO109I HaeII
Hin4I HincII HindIII KpnI MscI MslI MspA1I PasI
PflMI PfoI PspOMI PstI PvuII SacI SexAI SfcI
SfiI SmaI SmlI SrfI StyI TaqII XcmI XmaI
Enzymes that do not cut:
AatII AclI AfeI AflIII AhdI AloI ApaLI ApoI
AscI AseI AsiSI AvrII BamHI BbeI BcgI BciVI
BclI BfrBI BglII BmgBI BmrI BmtI BplI BpuEI
BsaAI BsaHI BsiEI BsmI BsmBI BspEI BspHI BsrBI
BsrGI BssHII BssSI BstBI BstYI BtgZI ClaI CspCI
DraI DrdI EagI EarI EciI EcoRI EcoRV FalI
FseI FspI FspAI HpaI KasI MfeI MluI MmeI
NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI
NsiI NspI PacI PciI PmeI PmlI PpiI PpuMI
PshAI PsiI PspXI PsrI PvuI RsrII SacII SalI
SanDI SapI SbfI ScaI SfoI SgfI SgrAI SnaBI
SpeI SphI SspI StuI SwaI TatI TstI Tth111I
XbaI XhoI XmnI ZraI