Restriction map for
pFN21AE9409
(Linear) (Six-base) MAPSORT of: KIEE9409.seq Check: 5931 from: 1 to: 1083
KIEE9409.orf
With 182 enzymes: SgfI *
December 27, 2011 16:39 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 886 1083
Size: 886 197
AccI GT'mk_AC
Cuts at: 0 119 527 1083
Size: 119 408 556
Fragments arranged by size:
556 408 119
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 762 1083
Size: 762 321
AleI CACnn'nnGTG
Cuts at: 0 757 1083
Size: 757 326
AlwNI CAG_nnn'CTG
Cuts at: 0 546 1083
Size: 546 537
ApaI G_GGCC'C
Cuts at: 0 208 714 1053 1083
Size: 208 506 339 30
Fragments arranged by size:
506 339 208 30
AvaI C'yCGr_G
Cuts at: 0 30 54 433 947 1083
Size: 30 24 379 514 136
Fragments arranged by size:
514 379 136 30 24
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 215 248 1083
Size: 215 33 835
Fragments arranged by size:
835 215 33
BanII G_rGCy'C
Cuts at: 0 17 25 208 584 714 766 948 1053
Size: 17 8 183 376 130 52 182 105
Cuts at: 1053 1083
Size: 30
Fragments arranged by size:
376 183 182 130 105 52 30 17
8
BbsI GAAGACnn'nnnn_
Cuts at: 0 789 1083
Size: 789 294
BbvCI CC'TCA_GC
Cuts at: 0 604 1083
Size: 604 479
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 429 463 688 720 722 754 1083
Size: 429 34 225 32 2 32 329
Fragments arranged by size:
429 329 225 34 32 32 2
BciVI GTATCCnnnnn_n'
Cuts at: 0 216 1083
Size: 216 867
BglI GCCn_nnn'nGGC
Cuts at: 0 164 292 572 790 1083
Size: 164 128 280 218 293
Fragments arranged by size:
293 280 218 164 128
BlpI GC'TnA_GC
Cuts at: 0 340 1002 1083
Size: 340 662 81
Fragments arranged by size:
662 340 81
Bme1580I G_kGCm'C
Cuts at: 0 208 364 714 838 1053 1083
Size: 208 156 350 124 215 30
Fragments arranged by size:
350 215 208 156 124 30
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 600 1083
Size: 600 483
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 39 91 100 1002 1083
Size: 39 52 9 902 81
Fragments arranged by size:
902 81 52 39 9
Bpu10I CC'TnA_GC
Cuts at: 0 604 1083
Size: 604 479
BsaAI yAC'GTr
Cuts at: 0 702 1083
Size: 702 381
BsaHI Gr'CG_yC
Cuts at: 0 485 1083
Size: 485 598
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 88 592 999 1083
Size: 88 504 407 84
Fragments arranged by size:
504 407 88 84
BseYI C'CCAG_C
Cuts at: 0 25 162 857 1083
Size: 25 137 695 226
Fragments arranged by size:
695 226 137 25
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 220 1083
Size: 220 863
BsiEI CG_ry'CG
Cuts at: 0 95 309 694 981 1083
Size: 95 214 385 287 102
Fragments arranged by size:
385 287 214 102 95
BsiHKAI G_wGCw'C
Cuts at: 0 269 754 1083
Size: 269 485 329
Fragments arranged by size:
485 329 269
Bsp1286I G_dGCh'C
Cuts at: 0 17 25 208 269 364 584 714 754
Size: 17 8 183 61 95 220 130 40
Cuts at: 754 766 838 948 1053 1083
Size: 12 72 110 105 30
Fragments arranged by size:
220 183 130 110 105 95 72 61
40 30 17 12 8
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 110 886 1083
Size: 110 776 197
Fragments arranged by size:
776 197 110
BsrBI CCG'CTC
Cuts at: 0 912 935 1083
Size: 912 23 148
Fragments arranged by size:
912 148 23
BsrDI GCAATG_nn'
Cuts at: 0 869 1083
Size: 869 214
BsrFI r'CCGG_y
Cuts at: 0 508 976 1083
Size: 508 468 107
Fragments arranged by size:
508 468 107
BssHII G'CGCG_C
Cuts at: 0 377 464 1083
Size: 377 87 619
Fragments arranged by size:
619 377 87
BssSI C'ACGA_G
Cuts at: 0 474 1083
Size: 474 609
BstAPI GCAn_nnn'nTGC
Cuts at: 0 368 546 1083
Size: 368 178 537
Fragments arranged by size:
537 368 178
BstXI CCAn_nnnn'nTGG
Cuts at: 0 882 1083
Size: 882 201
BtgI C'CryG_G
Cuts at: 0 152 208 287 419 494 1083
Size: 152 56 79 132 75 589
Fragments arranged by size:
589 152 132 79 75 56
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 338 1083
Size: 338 745
BtsI GCAGTG_nn'
Cuts at: 0 445 1064 1083
Size: 445 619 19
Fragments arranged by size:
619 445 19
EaeI y'GGCC_r
Cuts at: 0 92 191 306 313 416 506 691 978
Size: 92 99 115 7 103 90 185 287
Cuts at: 978 1083
Size: 105
Fragments arranged by size:
287 185 115 105 103 99 92 90
7
EagI C'GGCC_G
Cuts at: 0 92 306 691 978 1083
Size: 92 214 385 287 105
Fragments arranged by size:
385 287 214 105 92
EarI CTCTTCn'nnn_
Cuts at: 0 661 1083
Size: 661 422
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 39 91 100 762 1002 1083
Size: 39 52 9 662 240 81
Fragments arranged by size:
662 240 81 52 39 9
EcoO109I rG'GnC_Cy
Cuts at: 0 99 204 600 861 962 1083
Size: 99 105 396 261 101 121
Fragments arranged by size:
396 261 121 105 101 99
FseI GG_CCGG'CC
Cuts at: 0 512 1083
Size: 512 571
FspI TGC'GCA
Cuts at: 0 451 730 1083
Size: 451 279 353
Fragments arranged by size:
451 353 279
HaeII r_GCGC'y
Cuts at: 0 43 301 502 785 823 853 955 1083
Size: 43 258 201 283 38 30 102 128
Fragments arranged by size:
283 258 201 128 102 43 38 30
HincII GTy'rAC
Cuts at: 0 894 1083
Size: 894 189
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 41 1083
Size: 41 1042
MslI CAynn'nnrTG
Cuts at: 0 400 757 1083
Size: 400 357 326
Fragments arranged by size:
400 357 326
MspA1I CmG'CkG
Cuts at: 0 210 250 279 289 311 828 899 1083
Size: 210 40 29 10 22 517 71 184
Fragments arranged by size:
517 210 184 71 40 29 22 10
NaeI GCC'GGC
Cuts at: 0 510 1083
Size: 510 573
NgoMIV G'CCGG_C
Cuts at: 0 508 1083
Size: 508 575
NotI GC'GGCC_GC
Cuts at: 0 306 1083
Size: 306 777
PasI CC'CwG_GG
Cuts at: 0 44 1083
Size: 44 1039
PmlI CAC'GTG
Cuts at: 0 702 1083
Size: 702 381
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1054 1083
Size: 1054 29
PpuMI rG'GwC_Cy
Cuts at: 0 99 1083
Size: 99 984
PspOMI G'GGCC_C
Cuts at: 0 204 710 1049 1083
Size: 204 506 339 34
Fragments arranged by size:
506 339 204 34
PspXI vC'TCGA_Gb
Cuts at: 0 433 1083
Size: 433 650
PvuII CAG'CTG
Cuts at: 0 250 899 1083
Size: 250 649 184
Fragments arranged by size:
649 250 184
SacII CC_GC'GG
Cuts at: 0 211 290 1083
Size: 211 79 793
Fragments arranged by size:
793 211 79
SmaI CCC'GGG
Cuts at: 0 32 949 1083
Size: 32 917 134
Fragments arranged by size:
917 134 32
SmlI C'TyrA_G
Cuts at: 0 433 1083
Size: 433 650
SrfI GCCC'GGGC
Cuts at: 0 949 1083
Size: 949 134
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 553 585 1083
Size: 553 32 498
Fragments arranged by size:
553 498 32
XhoI C'TCGA_G
Cuts at: 0 433 1083
Size: 433 650
XmaI C'CCGG_G
Cuts at: 0 30 947 1083
Size: 30 917 136
Fragments arranged by size:
917 136 30
Enzymes that do cut:
AarI AccI AcuI AleI AlwNI ApaI AvaI BaeI
BanII BbsI BbvCI BcgI BciVI BglI BlpI Bme1580I
BplI BpmI Bpu10I BsaAI BsaHI BseRI BseYI BsgI
BsiEI BsiHKAI Bsp1286I BspMI BsrBI BsrDI BsrFI BssHII
BssSI BstAPI BstXI BtgI BtgZI BtsI EaeI EagI
EarI Eco57MI EcoO109I FseI FspI HaeII HincII MmeI
MslI MspA1I NaeI NgoMIV NotI PasI PmlI PpiI
PpuMI PspOMI PspXI PvuII SacII SmaI SmlI SrfI
TstI XhoI XmaI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII AflIII AgeI AhdI
AlfI AloI ApaLI ApoI AscI AseI AsiSI AvrII
BamHI BanI BbeI BclI BfrBI BglII BmgBI BmrI
BmtI BpuEI BsaI BsaBI BsaWI BsaXI BsiWI BsmI
BsmBI BspEI BspHI BsrGI BstBI BstEII BstYI BstZ17I
Bsu36I ClaI CspCI DraI DraIII DrdI EciI EcoICRI
EcoNI EcoRI EcoRV FalI FspAI Hin4I HindIII HpaI
KasI KpnI MfeI MluI MscI NarI NcoI NdeI
NheI NruI NsiI NspI PacI PciI PflMI PfoI
PmeI PshAI PsiI PsrI PstI PvuI RsrII SacI
SalI SanDI SapI SbfI ScaI SexAI SfcI SfiI
SfoI SgfI SgrAI SnaBI SpeI SphI SspI StuI
StyI SwaI TaqII TatI Tth111I XbaI XcmI XmnI
ZraI