Restriction map for
pFN21AE9406
(Linear) (Six-base) MAPSORT of: KIEE9406.seq Check: 888 from: 1 to: 1410
KIEE9406.orf
With 182 enzymes: SgfI *
December 27, 2011 16:39 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 673 1257 1410
Size: 673 584 153
Fragments arranged by size:
673 584 153
AatII G_ACGT'C
Cuts at: 0 800 1410
Size: 800 610
AccI GT'mk_AC
Cuts at: 0 800 833 1410
Size: 800 33 577
Fragments arranged by size:
800 577 33
Acc65I G'GTAC_C
Cuts at: 0 413 1410
Size: 413 997
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 468 1373 1410
Size: 468 905 37
Fragments arranged by size:
905 468 37
AflIII A'CryG_T
Cuts at: 0 190 1410
Size: 190 1220
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 966 1410
Size: 966 444
AlwNI CAG_nnn'CTG
Cuts at: 0 1204 1274 1410
Size: 1204 70 136
Fragments arranged by size:
1204 136 70
ApaI G_GGCC'C
Cuts at: 0 1323 1410
Size: 1323 87
AvaI C'yCGr_G
Cuts at: 0 508 1392 1410
Size: 508 884 18
Fragments arranged by size:
884 508 18
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 397 430 1410
Size: 397 33 980
Fragments arranged by size:
980 397 33
BamHI G'GATC_C
Cuts at: 0 1056 1104 1152 1410
Size: 1056 48 48 258
Fragments arranged by size:
1056 258 48 48
BanI G'GyrC_C
Cuts at: 0 28 413 422 1410
Size: 28 385 9 988
Fragments arranged by size:
988 385 28 9
BanII G_rGCy'C
Cuts at: 0 1323 1410
Size: 1323 87
BbsI GAAGACnn'nnnn_
Cuts at: 0 93 495 1410
Size: 93 402 915
Fragments arranged by size:
915 402 93
BbvCI CC'TCA_GC
Cuts at: 0 373 577 868 1410
Size: 373 204 291 542
Fragments arranged by size:
542 373 291 204
BglI GCCn_nnn'nGGC
Cuts at: 0 712 1410
Size: 712 698
BlpI GC'TnA_GC
Cuts at: 0 211 1115 1410
Size: 211 904 295
Fragments arranged by size:
904 295 211
Bme1580I G_kGCm'C
Cuts at: 0 406 425 494 1268 1323 1410
Size: 406 19 69 774 55 87
Fragments arranged by size:
774 406 87 69 55 19
BmgBI CAC'GTC
Cuts at: 0 193 1410
Size: 193 1217
BmrI ACTGGGnnnn_n'
Cuts at: 0 424 1038 1262 1410
Size: 424 614 224 148
Fragments arranged by size:
614 424 224 148
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 607 1075 1410
Size: 607 468 335
Fragments arranged by size:
607 468 335
Bpu10I CC'TnA_GC
Cuts at: 0 373 577 868 1410
Size: 373 204 291 542
Fragments arranged by size:
542 373 291 204
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1345 1410
Size: 1345 65
BsaAI yAC'GTr
Cuts at: 0 636 1410
Size: 636 774
BsaHI Gr'CG_yC
Cuts at: 0 797 1410
Size: 797 613
BsaWI w'CCGG_w
Cuts at: 0 905 1410
Size: 905 505
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 336 580 970 1410
Size: 336 244 390 440
Fragments arranged by size:
440 390 336 244
BseYI C'CCAG_C
Cuts at: 0 89 294 767 995 1067 1139 1163 1187
Size: 89 205 473 228 72 72 24 24
Cuts at: 1187 1211 1332 1366 1410
Size: 24 121 34 44
Fragments arranged by size:
473 228 205 121 89 72 72 44
34 24 24 24
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1229 1410
Size: 1229 181
BsiEI CG_ry'CG
Cuts at: 0 384 964 1102 1410
Size: 384 580 138 308
Fragments arranged by size:
580 384 308 138
BsiHKAI G_wGCw'C
Cuts at: 0 641 929 1410
Size: 641 288 481
Fragments arranged by size:
641 481 288
Bsp1286I G_dGCh'C
Cuts at: 0 406 425 494 641 929 1268 1323 1410
Size: 406 19 69 147 288 339 55 87
Fragments arranged by size:
406 339 288 147 87 69 55 19
BspEI T'CCGG_A
Cuts at: 0 905 1410
Size: 905 505
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 673 821 1257 1410
Size: 673 148 436 153
Fragments arranged by size:
673 436 153 148
BsrBI CCG'CTC
Cuts at: 0 960 1410
Size: 960 450
BsrFI r'CCGG_y
Cuts at: 0 160 457 1410
Size: 160 297 953
Fragments arranged by size:
953 297 160
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1252 1410
Size: 1252 158
BstYI r'GATC_y
Cuts at: 0 260 269 1056 1104 1152 1410
Size: 260 9 787 48 48 258
Fragments arranged by size:
787 260 258 48 48 9
Bsu36I CC'TnA_GG
Cuts at: 0 745 1073 1410
Size: 745 328 337
Fragments arranged by size:
745 337 328
BtgI C'CryG_G
Cuts at: 0 11 1410
Size: 11 1399
DrdI GACnn_nn'nnGTC
Cuts at: 0 726 1231 1410
Size: 726 505 179
Fragments arranged by size:
726 505 179
EaeI y'GGCC_r
Cuts at: 0 36 158 289 365 473 713 890 961
Size: 36 122 131 76 108 240 177 71
Cuts at: 961 1410
Size: 449
Fragments arranged by size:
449 240 177 131 122 108 76 71
36
EagI C'GGCC_G
Cuts at: 0 961 1410
Size: 961 449
EarI CTCTTCn'nnn_
Cuts at: 0 361 487 1410
Size: 361 126 923
Fragments arranged by size:
923 361 126
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 424 1410
Size: 424 986
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 468 607 1075 1373 1410
Size: 468 139 468 298 37
Fragments arranged by size:
468 468 298 139 37
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1313 1410
Size: 1313 97
EcoO109I rG'GnC_Cy
Cuts at: 0 44 591 825 1319 1320 1329 1402 1410
Size: 44 547 234 494 1 9 73 8
Fragments arranged by size:
547 494 234 73 44 9 8 1
HaeII r_GCGC'y
Cuts at: 0 202 400 1410
Size: 202 198 1010
Fragments arranged by size:
1010 202 198
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 55 87 556 588 787 819 1030 1062
Size: 55 32 469 32 199 32 211 32
Cuts at: 1062 1410
Size: 348
Fragments arranged by size:
469 348 211 199 55 32 32 32
32
HindIII A'AGCT_T
Cuts at: 0 1283 1410
Size: 1283 127
KpnI G_GTAC'C
Cuts at: 0 417 1410
Size: 417 993
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 863 1039 1130 1410
Size: 863 176 91 280
Fragments arranged by size:
863 280 176 91
MscI TGG'CCA
Cuts at: 0 38 475 715 892 1410
Size: 38 437 240 177 518
Fragments arranged by size:
518 437 240 177 38
MslI CAynn'nnrTG
Cuts at: 0 10 1410
Size: 10 1400
MspA1I CmG'CkG
Cuts at: 0 250 294 304 386 762 771 863 1125
Size: 250 44 10 82 376 9 92 262
Cuts at: 1125 1173 1410
Size: 48 237
Fragments arranged by size:
376 262 250 237 92 82 48 44
10 9
NaeI GCC'GGC
Cuts at: 0 162 1410
Size: 162 1248
NcoI C'CATG_G
Cuts at: 0 11 1410
Size: 11 1399
NgoMIV G'CCGG_C
Cuts at: 0 160 1410
Size: 160 1250
PasI CC'CwG_GG
Cuts at: 0 418 1315 1410
Size: 418 897 95
Fragments arranged by size:
897 418 95
PflMI CCAn_nnn'nTGG
Cuts at: 0 139 1410
Size: 139 1271
PmlI CAC'GTG
Cuts at: 0 636 1410
Size: 636 774
PpuMI rG'GwC_Cy
Cuts at: 0 591 1329 1410
Size: 591 738 81
Fragments arranged by size:
738 591 81
PspOMI G'GGCC_C
Cuts at: 0 1319 1410
Size: 1319 91
PvuI CG_AT'CG
Cuts at: 0 1102 1410
Size: 1102 308
PvuII CAG'CTG
Cuts at: 0 304 762 771 863 1125 1173 1410
Size: 304 458 9 92 262 48 237
Fragments arranged by size:
458 304 262 237 92 48 9
SexAI A'CCwGG_T
Cuts at: 0 533 1410
Size: 533 877
SgrAI Cr'CCGG_yG
Cuts at: 0 457 1410
Size: 457 953
SmaI CCC'GGG
Cuts at: 0 1394 1410
Size: 1394 16
SmlI C'TyrA_G
Cuts at: 0 1324 1410
Size: 1324 86
StyI C'CwwG_G
Cuts at: 0 11 81 123 1410
Size: 11 70 42 1287
Fragments arranged by size:
1287 70 42 11
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 16 456 658 1410
Size: 16 440 202 752
Fragments arranged by size:
752 440 202 16
Tth111I GACn'n_nGTC
Cuts at: 0 97 535 1410
Size: 97 438 875
Fragments arranged by size:
875 438 97
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 144 175 1410
Size: 144 31 1235
Fragments arranged by size:
1235 144 31
XmaI C'CCGG_G
Cuts at: 0 1392 1410
Size: 1392 18
ZraI GAC'GTC
Cuts at: 0 798 1410
Size: 798 612
Enzymes that do cut:
AarI AatII AccI Acc65I AcuI AflIII AlfI AlwNI
ApaI AvaI BaeI BamHI BanI BanII BbsI BbvCI
BglI BlpI Bme1580I BmgBI BmrI BpmI Bpu10I BpuEI
BsaAI BsaHI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI
Bsp1286I BspEI BspMI BsrBI BsrFI BstAPI BstYI Bsu36I
BtgI DrdI EaeI EagI EarI EciI Eco57MI EcoNI
EcoO109I HaeII Hin4I HindIII KpnI MmeI MscI MslI
MspA1I NaeI NcoI NgoMIV PasI PflMI PmlI PpuMI
PspOMI PvuI PvuII SexAI SgrAI SmaI SmlI StyI
TaqII Tth111I XcmI XmaI ZraI
Enzymes that do not cut:
AclI AfeI AflII AgeI AhdI AleI AloI ApaLI
ApoI AscI AseI AsiSI AvrII BbeI BcgI BciVI
BclI BfrBI BglII BmtI BplI BsaI BsaBI BsaXI
BsiWI BsmI BsmBI BspHI BsrDI BsrGI BssHII BssSI
BstBI BstEII BstXI BstZ17I BtgZI BtsI ClaI CspCI
DraI DraIII EcoICRI EcoRI EcoRV FalI FseI FspI
FspAI HincII HpaI KasI MfeI MluI NarI NdeI
NheI NotI NruI NsiI NspI PacI PciI PfoI
PmeI PpiI PshAI PsiI PspXI PsrI PstI RsrII
SacI SacII SalI SanDI SapI SbfI ScaI SfcI
SfiI SfoI SgfI SnaBI SpeI SphI SrfI SspI
StuI SwaI TatI TstI XbaI XhoI XmnI